Reviewed, UniProtKB/Swiss-Prot Q7MHA7 (CYSH_VIBVY)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	VV2965

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite.
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Phosphoadenosine phosphosulfate reductase		PRO_0000100655

Sequences

	Sequence		Length	Mass (Da)
	Q7MHA7-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 4E006F7192921972	FASTA	258	29,462
	10 20 30 40 50 60 MLDSVASTLQ LSELLSLTKA EQSIRLAEIN VELEMLSAQE RVAWALQNLE GAHAVSSSFG 70 80 90 100 110 120 IQAAVMLHLL SKQQADIPVI LTDTGYLFPE TYQFIDELTK SLNLNLKVYR ANESANWQEA 130 140 150 160 170 180 RYGKLWEQGI EGIEKYNKLN KVEPMRRALN ELNVKTWFSG LRREQSQSRA GLPILSIQNG 190 200 210 220 230 240 VFKFLPVVDW SNKDVHYYLK EHGLSYHPLW EQGYLSVGDT HTTQKWEPGM SEEETRFFGL 250 KRECGLHEED NEQDGSGI

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000037 Genomic DNA. Translation: BAC95729.1. Different initiation.
RefSeq	NP_935758.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2625814.
GenomeReviews	Gene locus VV2965 in contig BA000037_GR.
KEGG	vvy:VV2965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MHA7.
Enzyme and pathway databases
BioCyc	VVUL196600:VV2965-MON.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_VIBVY

Accession

Primary (citable) accession number: Q7MHA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)