Reviewed, UniProtKB/Swiss-Prot Q7MHA6 (CYSI_VIBVY)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	VV2966

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity. HAMAP MF_01540
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000199916
Sites
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	447	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	487	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	491	1	Iron (siroheme axial ligand) By similarity
	Metal binding	491	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MHA6-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 89E49E27342E7C5B	FASTA	578	64,959
	10 20 30 40 50 60 MTFSIENNKQ VVLGEELGKL SDNERLKTQS NFLRGTIEQD LQDRITGGFT ADNFQLIRFH 70 80 90 100 110 120 GMYQQDDRDI RNERAKQKLE PLHNVMLRAR MPGGIITPKQ WLAIDKFATE HSLYGSIRLT 130 140 150 160 170 180 TRQTFQFHGV LKPNIKLMHQ TLNSIGIDSI ATAGDVNRNV LCTTNPVESE LHQEAYEWAK 190 200 210 220 230 240 KISEHLLPKT KAYAEIWLDG EKVETTEDDE PILGKTYLPR KFKTTVVIPP QNDVDVHAND 250 260 270 280 290 300 LNFVAIAENG KLIGFNVLVG GGLAMTHGDT STYPRRADDF GYIPLEKTLD VAAAVVTTQR 310 320 330 340 350 360 DWGNRSNRKN AKTKYTLDRV GTDVFKAEVE KRAGIKFEVS RPYEFTERGD RIGWVEGIDG 370 380 390 400 410 420 KHHLTLFIEN GRLLDYPGKP LKTGVAEIAK IHKGDFRMTA NQNLIVAGVS KSNKAKIEKL 430 440 450 460 470 480 AREHGLMDEG VSEQRKNSMA CVAFPTCPLA MAEAERFLPQ FVTDVEGILD KHGLDKEDNI 490 500 510 520 530 540 ILRVTGCPNG CGRAMLAEIG LVGKAPGRYN LHLGGNRGGT RVPKMYKENI TEKQILEEID 550 560 570 QLVGRWANER LPNECFGDFT VRVGIIEEVI ISKRDFYA

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000037 Genomic DNA. Translation: BAC95730.1.
RefSeq	NP_935759.1.
3D structure databases
SMR	Q7MHA6. Positions 86-577.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MHA6.
Genome annotation databases
GeneID	2625815.
GenomeReviews	Gene locus VV2966 in contig BA000037_GR.
KEGG	vvy:VV2966.
NMPDR	fig|196600.1.peg.3032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0155.
HOGENOM	HBG626671.
OMA	PGDNSVD.
Enzyme and pathway databases
BioCyc	VVUL196600:VV2966-MONOMER.
Family and domain databases
HAMAP	MF_01540. CysI. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_VIBVY

Accession

Primary (citable) accession number: Q7MHA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)