Reviewed,
UniProtKB/Swiss-Prot Q7MH09 (HMP_VIBVY)
Last modified
November 25, 2008.
Version 34.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Flavohemoprotein Alternative name(s): Hemoglobin-like protein Flavohemoglobin Nitric oxide dioxygenase Short name=NO oxygenase Short name=NOD EC=1.14.12.17 | ||||
| Gene names |
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| Organism | Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 196600 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio |
Protein attributes
| Sequence length | 394 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress By similarity. |
| Catalytic activity | 2 NO + 2 O(2) + NAD(P)H = 2 NO(3)(-) + NAD(P)(+). |
| Cofactor | Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 1 FAD per subunit By similarity. |
| Domain | Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H. |
| Sequence similarities | Belongs to the globin family. Two-domain flavohemoproteins subfamily. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Detoxification Oxygen transport Transport |
| Ligand | FAD Flavoprotein Heme Iron Metal-binding NAD NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxygen transportInferred from electronic annotation. Source: HAMAP response to toxinInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | electron carrier activity Inferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro nitric oxide dioxygenase activityInferred from electronic annotation. Source: HAMAP oxygen bindingInferred from electronic annotation. Source: InterPro oxygen transporter activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 394 | 394 | Flavohemoprotein | PRO_0000052452 | |||||
Regions | |||||||||
| Domain | 150 – 255 | 106 | FAD-binding FR-type | ||||||
| Nucleotide binding | 204 – 207 | 4 | FAD By similarity | ||||||
| Nucleotide binding | 268 – 273 | 6 | NADP By similarity | ||||||
| Nucleotide binding | 387 – 390 | 4 | FAD By similarity | ||||||
| Region | 1 – 136 | 136 | Globin | ||||||
| Region | 147 – 394 | 248 | Reductase | ||||||
| Region | 259 – 394 | 136 | NAD or NADP-binding | ||||||
Sites | |||||||||
| Active site | 95 | 1 | Charge relay system By similarity | ||||||
| Active site | 135 | 1 | Charge relay system By similarity | ||||||
| Metal binding | 85 | 1 | Iron (heme proximal ligand) By similarity | ||||||
| Binding site | 188 | 1 | FAD By similarity | ||||||
| Site | 29 | 1 | Involved in heme-bound ligand stabilization and O-O bond activation By similarity | ||||||
| Site | 84 | 1 | Influences the redox potential of the prosthetic heme and FAD groups By similarity | ||||||
| Site | 386 | 1 | Influences the redox potential of the prosthetic heme and FAD groups By similarity | ||||||
Sequences
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References
| [1] | "Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| BA000037 Genomic DNA. Translation: BAC95828.1. | |
| RefSeq | NP_935857.1. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2625913. |
| GenomeReviews | Gene locus VV3064 in contig BA000037_GR. |
| KEGG | vvy:VV3064. |
| NMPDR | fig|196600.1.peg.3130. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q7MH09. |
Enzyme and pathway databases | |
| BioCyc | VVUL196600:VV3064-MON. |
Family and domain databases | |
| HAMAP | MF_01252. [Tree] |
| InterPro | IPR001709. FPN_cyt_redctse. IPR012292. Globin. IPR000971. Globin_subset. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR001221. Phe_hydroxylase. [Graphical view] |
| Gene3D | G3DSA:1.10.490.10. Globin_related. 1 hit. |
| Pfam | PF00970. FAD_binding_6. 1 hit. PF00042. Globin. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view] |
| PRINTS | PR00371. FPNCR. PR00410. PHEHYDRXLASE. |
| PROSITE | PS51384. FAD_FR. 1 hit. PS01033. GLOBIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HMP_VIBVY | ||||||||
| Accession | Primary (citable) accession number: Q7MH09 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
