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Q7MGT4 (PUR2_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:VV3143
OrganismVibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]
Taxonomic identifier196600 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence BAC95907.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151500

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MGT4 [UniParc].

Last modified December 21, 2004. Version 2.
Checksum: F2E886F3891C916B

FASTA42945,744
        10         20         30         40         50         60 
MNVLIIGSGG REHALGWKAA QNPNVETIFV APGNAGTALE PKLENVNIAV EDIAGLVAFA 

        70         80         90        100        110        120 
KEKAIELTIV GPEVPLVLGV VDAFYEAGLP IFGPTQAAAQ LEGSKAFTKD FLARHQIPTA 

       130        140        150        160        170        180 
AYANFTDIEP ALAYVREQGA PIVVKADGLA AGKGVIVAMT LEEAEEAIKD MLAGNAFGEA 

       190        200        210        220        230        240 
GSRVVIEEFL DGEEASFIVM VDGENVLPMA TSQDHKRVGD KDTGPNTGGM GAYSPAPVVT 

       250        260        270        280        290        300 
PEIHNRVMQE VIFPTVRGMA AEGNPYTGFL YAGLMIDKDG TPKVIEYNCR FGDPETQPIM 

       310        320        330        340        350        360 
MRMESDLVEL CLAAIDKKLD QVESKWDPRA SIGIVLAAGG YPAAYNKGDV ISGLPQVEIE 

       370        380        390        400        410        420 
GEKVFHAGTE NKGGDIVTNG GRVLCATALG NSVSEAQQRA YELAKQIRWD GMFHRNDIGY 


RAIAREQQK 

« Hide

References

[1]"Comparative genome analysis of Vibrio vulnificus, a marine pathogen."
Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F.
Genome Res. 13:2577-2587(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YJ016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000037 Genomic DNA. Translation: BAC95907.1. Different initiation.
RefSeqNP_935936.1. NC_005139.1.

3D structure databases

ProteinModelPortalQ7MGT4.
SMRQ7MGT4. Positions 1-425.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196600.VV3143.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC95907; BAC95907; BAC95907.
GeneID2626003.
KEGGvvy:VV3143.
PATRIC20173166. VBIVibVul40472_3115.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycVVUL196600:GJ9W-3252-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_VIBVY
AccessionPrimary (citable) accession number: Q7MGT4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: February 19, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways