Reviewed, UniProtKB/Swiss-Prot Q7MGL1 (PURK_VIBVY)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	VV3219

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075019
Regions
	Domain	97 – 287	191	ATP-grasp
	Nucleotide binding	175 – 178	4	ATP By similarity
	Nucleotide binding	257 – 258	2	ATP By similarity
Sites
	Binding site	93	1	ATP By similarity
	Binding site	133	1	ATP By similarity
	Binding site	144	1	ATP By similarity
	Binding site	183	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MGL1-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 4444CB6A1A2F9F9D	FASTA	377	41,277
	10 20 30 40 50 60 MHVLVLGAGQ LARMMSLAGA PLNIQISAYD VTTGDVVHPL TLHLLGHGLE QAIEHVDVIT 70 80 90 100 110 120 AEFEHIPHDI LAICQASGKF LPSSEAIKAG GDRRLEKTLL DHAGVRNANY YVIETREDFN 130 140 150 160 170 180 KAIEHVGIPM VLKSALGGYD GRGQWRLKDA AQIETLWQEM AACIAATPTQ AIVAEEFVPF 190 200 210 220 230 240 QREVSLIGAR GKEGQIEVYP LAENIHVNGV LSLSTAIDSP DLQEQAKHMF TAVAETLNYV 250 260 270 280 290 300 GVLALEFFDV DGQLLVNEIA PRVHNSGHWT QQGAETCQFE NHLRAVCGLP LGSTKLVRET 310 320 330 340 350 360 SMINILGEDT LPASVMAMDG CHIHWYGKEK RAGRKMGHIN VCGDYSGELQ RRLCALANVL 370 DEKAFPAVHE FAKKWQA

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000037 Genomic DNA. Translation: BAC95983.1.
RefSeq	NP_936012.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6R based on UniProtKB P09029.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MGL1.
Genome annotation databases
GeneID	2626095.
GenomeReviews	Gene locus VV3219 in contig BA000037_GR.
KEGG	vvy:VV3219.
NMPDR	fig|196600.1.peg.3285.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MGL1.
OMA	RKIGHVN.
Enzyme and pathway databases
BioCyc	VVUL196600:VV3219-MON.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_VIBVY

Accession

Primary (citable) accession number: Q7MGL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)