Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei396 – 3961NucleophileUniRule annotation
Active sitei449 – 4491Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-3466-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:VVA0079
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002675: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7157151,4-alpha-glucan branching enzyme GlgBPRO_0000188763Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196600.VVA0079.

Structurei

3D structure databases

ProteinModelPortaliQ7MG90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7MG90-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFEKLSQAAC SEPFAFLGPF IDPTQGALRV WMPGATGVAL VLEGQPRIAL
60 70 80 90 100
EREKESAFIL KADLNLHLTH YQLAIDWNGI EQLVDDPYQY HGIYAEYDDL
110 120 130 140 150
HTPKTMYQHM GSQFMTLERD GKSISGIRFL VYAPHATAVS LVGSFNDWDG
160 170 180 190 200
RRHPMQRLDY GIWGLFIPGL TEGVSYKFEM KGPKGEGLPH KADPWGFYAE
210 220 230 240 250
QYPSFASVTY DHARYQWQDA QWQTRPVTEK RKEALSFYEL HAGSWKRNEQ
260 270 280 290 300
GEFLNYRELA AELVPYLVDM GYTHVELMPV SEHPFYGSWG YQPVGLFAPT
310 320 330 340 350
SRYGSPDDFK FFVDACHQAG IGVVLDWVPA HFPSDDHGLA NFDGTPLFHD
360 370 380 390 400
PDPRRGWHQD WNSFIYDLGR EQVRRFLVSN ALYWFEQFHI DGIRVDAVAS
410 420 430 440 450
MLYLDYSRSH GQWIPNMDGG NENYDAIATL KWMNEEVYKY FPNAMTIAEE
460 470 480 490 500
STAFPGVSAP TFMGGLGFGF KWNMGWMHDS LSYIKEEPVH RKYHHNTLTF
510 520 530 540 550
PLVYAHSENY VLSLSHDEVV YGKGSIHNKM PGDEWQQTAN LRAYFGYMYG
560 570 580 590 600
QPGKKLNFMG AEIGQTAEWN HDDQLQWFLL DFPRHQGVQA LTRDLNHLYR
610 620 630 640 650
NEAALHDQDC IPAGFEWRLQ DAAEQSIIAH ERISEAGERI LVVSNFTPVP
660 670 680 690 700
RDEFRLGVPN KGRYQLLLNT DDSKYAGSGY EVVVDVKSEA VVSEDLAQSI
710
VLRLPPLSTL FYKLA
Length:715
Mass (Da):81,914
Last modified:May 10, 2005 - v2
Checksum:iDA9BDD72D7E70F66
GO

Sequence cautioni

The sequence BAC96105.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000038 Genomic DNA. Translation: BAC96105.1. Different initiation.
RefSeqiNP_936135.1. NC_005140.1.

Genome annotation databases

EnsemblBacteriaiBAC96105; BAC96105; BAC96105.
GeneIDi2621598.
KEGGivvy:VVA0079.
PATRICi20173570. VBIVibVul40472_3300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000038 Genomic DNA. Translation: BAC96105.1. Different initiation.
RefSeqiNP_936135.1. NC_005140.1.

3D structure databases

ProteinModelPortaliQ7MG90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196600.VVA0079.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC96105; BAC96105; BAC96105.
GeneIDi2621598.
KEGGivvy:VVA0079.
PATRICi20173570. VBIVibVul40472_3300.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciVVUL196600:GJ9W-3466-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YJ016.

Entry informationi

Entry nameiGLGB_VIBVY
AccessioniPrimary (citable) accession number: Q7MG90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: January 7, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.