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Q7MG04 (BGAL_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:VVA0165
OrganismVibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]
Taxonomic identifier196600 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length1031 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10311031Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367013

Regions

Region533 – 5364Substrate binding By similarity

Sites

Active site4571Proton donor By similarity
Active site5331Nucleophile By similarity
Metal binding1981Sodium By similarity
Metal binding4121Magnesium 1 By similarity
Metal binding4141Magnesium 1 By similarity
Metal binding4571Magnesium 1 By similarity
Metal binding5931Magnesium 2 By similarity
Metal binding5971Sodium; via carbonyl oxygen By similarity
Metal binding6001Sodium By similarity
Binding site1001Substrate By similarity
Binding site1981Substrate By similarity
Binding site4571Substrate By similarity
Binding site6001Substrate By similarity
Binding site10051Substrate By similarity
Site3531Transition state stabilizer By similarity
Site3871Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MG04 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 3A3E6A9AD4BC5560

FASTA1,031117,763
        10         20         30         40         50         60 
MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL NGQWKFKLFD 

        70         80         90        100        110        120 
APEQVDGQFT QADFNDAEWD EIPVPSNWQL HGYDKPIYAN IKYPFDVNPP FVPRENPTGC 

       130        140        150        160        170        180 
YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT 

       190        200        210        220        230        240 
LAVMVMRWCD GSYLEDQDMW WLSGIFRDVT LLSKPQHCIE DVFITPDLDA CYRDGSLSIV 

       250        260        270        280        290        300 
TTIAAPETYQ VQVQLFEGTQ AVTEPNIDRP HNRRIDERGT WNDVVFQTLH LREPKKWTAE 

       310        320        330        340        350        360 
TPNLYRLVVS LLDENGTHLE SEAYPVGFRK VEISEGQLKL NGKPLLIRGV NRHEHHPELG 

       370        380        390        400        410        420 
HVMTEEDMIR DICLMKQYNF NAVRTAHYPN HPRWYELCDQ YGLYVCDEAN IETHGMQPMS 

       430        440        450        460        470        480 
RLSSDPQWAH AYMSRYTQMV LRDKNHPSII IWSLGNESGH GSNHNAMYAW SKNFDPSRPV 

       490        500        510        520        530        540 
QYEGGGSNTT ATDIIAPMYA RVNTLIADEA VPKWPIKKWI SLPNETRPLI LCEYAHAMGN 

       550        560        570        580        590        600 
SLGSFDEYWA AFREFPRLQG GFIWDWVDQG LSQWDENGQH FWAYGGDFGD EINDRQFCIN 

       610        620        630        640        650        660 
GLIFPDRTVH PTLQEAKYCQ RMITVSLQEQ TQKACTLLVT NENLFRATDN EQLNWSLLEN 

       670        680        690        700        710        720 
GQVIQTGSLA LSVEADSQTR LEIALNFTPK AQAQYHLNTD ICLIEATSWA PAGHVVATEQ 

       730        740        750        760        770        780 
MALRNHAGLA IPTLRTQPAP KLTQDGHAIV VSSLDEKHQW RWDSQSGLLM EWNVDGKAQM 

       790        800        810        820        830        840 
LAAPQDNFFR APLDNDIGIS EVDNVDPNAW VCRWEMAGIG QWERHCVHCD SETLAHTVVV 

       850        860        870        880        890        900 
TTTFAYHFGG DVQAITQWTH TLSNDGEMLL DVDVTLADTL PPMPRIGLEL QLPLYQADTP 

       910        920        930        940        950        960 
ITWQGLGPFE NYPDRLAAAR FGLHTQTLAQ MHTPYIFPTD SGLRCGTQWL QVNELAISGD 

       970        980        990       1000       1010       1020 
FQFSVSQYAQ QQLAEAKHTH DLLAQERIYL RLDHQHMGVG GDDSWSPSVH KEFQLTEKHY 

      1030 
RYQLRFKPAS R 

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References

[1]"Comparative genome analysis of Vibrio vulnificus, a marine pathogen."
Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F.
Genome Res. 13:2577-2587(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YJ016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000038 Genomic DNA. Translation: BAC96191.1.
RefSeqNP_936221.1. NC_005140.1.

3D structure databases

ProteinModelPortalQ7MG04.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196600.VVA0165.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC96191; BAC96191; BAC96191.
GeneID2621483.
KEGGvvy:VVA0165.
PATRIC20173734. VBIVibVul40472_3382.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMANPPFVPK.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycVVUL196600:GJ9W-3552-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_VIBVY
AccessionPrimary (citable) accession number: Q7MG04
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries