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Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateUniRule annotation
Metal bindingi198 – 1981SodiumUniRule annotation
Binding sitei198 – 1981SubstrateUniRule annotation
Sitei353 – 3531Transition state stabilizerUniRule annotation
Sitei387 – 3871Transition state stabilizerUniRule annotation
Metal bindingi412 – 4121Magnesium 1UniRule annotation
Metal bindingi414 – 4141Magnesium 1UniRule annotation
Active sitei457 – 4571Proton donorUniRule annotation
Metal bindingi457 – 4571Magnesium 1UniRule annotation
Binding sitei457 – 4571SubstrateUniRule annotation
Active sitei533 – 5331NucleophileUniRule annotation
Metal bindingi593 – 5931Magnesium 2UniRule annotation
Metal bindingi597 – 5971Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi600 – 6001SodiumUniRule annotation
Binding sitei600 – 6001SubstrateUniRule annotation
Binding sitei1005 – 10051SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-3552-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:VVA0165
OrganismiVibrio vulnificus (strain YJ016)
Taxonomic identifieri196600 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002675 Componenti: Chromosome II

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10311031Beta-galactosidasePRO_0000367013Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ7MG04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni533 – 5364Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNIDAYWM.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MG04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL
60 70 80 90 100
NGQWKFKLFD APEQVDGQFT QADFNDAEWD EIPVPSNWQL HGYDKPIYAN
110 120 130 140 150
IKYPFDVNPP FVPRENPTGC YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW
160 170 180 190 200
CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT LAVMVMRWCD GSYLEDQDMW
210 220 230 240 250
WLSGIFRDVT LLSKPQHCIE DVFITPDLDA CYRDGSLSIV TTIAAPETYQ
260 270 280 290 300
VQVQLFEGTQ AVTEPNIDRP HNRRIDERGT WNDVVFQTLH LREPKKWTAE
310 320 330 340 350
TPNLYRLVVS LLDENGTHLE SEAYPVGFRK VEISEGQLKL NGKPLLIRGV
360 370 380 390 400
NRHEHHPELG HVMTEEDMIR DICLMKQYNF NAVRTAHYPN HPRWYELCDQ
410 420 430 440 450
YGLYVCDEAN IETHGMQPMS RLSSDPQWAH AYMSRYTQMV LRDKNHPSII
460 470 480 490 500
IWSLGNESGH GSNHNAMYAW SKNFDPSRPV QYEGGGSNTT ATDIIAPMYA
510 520 530 540 550
RVNTLIADEA VPKWPIKKWI SLPNETRPLI LCEYAHAMGN SLGSFDEYWA
560 570 580 590 600
AFREFPRLQG GFIWDWVDQG LSQWDENGQH FWAYGGDFGD EINDRQFCIN
610 620 630 640 650
GLIFPDRTVH PTLQEAKYCQ RMITVSLQEQ TQKACTLLVT NENLFRATDN
660 670 680 690 700
EQLNWSLLEN GQVIQTGSLA LSVEADSQTR LEIALNFTPK AQAQYHLNTD
710 720 730 740 750
ICLIEATSWA PAGHVVATEQ MALRNHAGLA IPTLRTQPAP KLTQDGHAIV
760 770 780 790 800
VSSLDEKHQW RWDSQSGLLM EWNVDGKAQM LAAPQDNFFR APLDNDIGIS
810 820 830 840 850
EVDNVDPNAW VCRWEMAGIG QWERHCVHCD SETLAHTVVV TTTFAYHFGG
860 870 880 890 900
DVQAITQWTH TLSNDGEMLL DVDVTLADTL PPMPRIGLEL QLPLYQADTP
910 920 930 940 950
ITWQGLGPFE NYPDRLAAAR FGLHTQTLAQ MHTPYIFPTD SGLRCGTQWL
960 970 980 990 1000
QVNELAISGD FQFSVSQYAQ QQLAEAKHTH DLLAQERIYL RLDHQHMGVG
1010 1020 1030
GDDSWSPSVH KEFQLTEKHY RYQLRFKPAS R
Length:1,031
Mass (Da):117,763
Last modified:December 15, 2003 - v1
Checksum:i3A3E6A9AD4BC5560
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000038 Genomic DNA. Translation: BAC96191.1.
RefSeqiWP_011151589.1. NC_005140.1.

Genome annotation databases

EnsemblBacteriaiBAC96191; BAC96191; BAC96191.
GeneIDi2621483.
KEGGivvy:VVA0165.
PATRICi20173734. VBIVibVul40472_3382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000038 Genomic DNA. Translation: BAC96191.1.
RefSeqiWP_011151589.1. NC_005140.1.

3D structure databases

ProteinModelPortaliQ7MG04.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC96191; BAC96191; BAC96191.
GeneIDi2621483.
KEGGivvy:VVA0165.
PATRICi20173734. VBIVibVul40472_3382.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNIDAYWM.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciVVUL196600:GJ9W-3552-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YJ016.

Entry informationi

Entry nameiBGAL_VIBVY
AccessioniPrimary (citable) accession number: Q7MG04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 15, 2003
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.