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Q7MG04

- BGAL_VIBVY

UniProt

Q7MG04 - BGAL_VIBVY

Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio vulnificus (strain YJ016)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001SubstrateUniRule annotation
    Metal bindingi198 – 1981SodiumUniRule annotation
    Binding sitei198 – 1981SubstrateUniRule annotation
    Sitei353 – 3531Transition state stabilizerUniRule annotation
    Sitei387 – 3871Transition state stabilizerUniRule annotation
    Metal bindingi412 – 4121Magnesium 1UniRule annotation
    Metal bindingi414 – 4141Magnesium 1UniRule annotation
    Active sitei457 – 4571Proton donorUniRule annotation
    Metal bindingi457 – 4571Magnesium 1UniRule annotation
    Binding sitei457 – 4571SubstrateUniRule annotation
    Active sitei533 – 5331NucleophileUniRule annotation
    Metal bindingi593 – 5931Magnesium 2UniRule annotation
    Metal bindingi597 – 5971Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi600 – 6001SodiumUniRule annotation
    Binding sitei600 – 6001SubstrateUniRule annotation
    Binding sitei1005 – 10051SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciVVUL196600:GJ9W-3552-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:VVA0165
    OrganismiVibrio vulnificus (strain YJ016)
    Taxonomic identifieri196600 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000002675: Chromosome II

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10311031Beta-galactosidasePRO_0000367013Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196600.VVA0165.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7MG04.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni533 – 5364Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7MG04-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL     50
    NGQWKFKLFD APEQVDGQFT QADFNDAEWD EIPVPSNWQL HGYDKPIYAN 100
    IKYPFDVNPP FVPRENPTGC YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW 150
    CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT LAVMVMRWCD GSYLEDQDMW 200
    WLSGIFRDVT LLSKPQHCIE DVFITPDLDA CYRDGSLSIV TTIAAPETYQ 250
    VQVQLFEGTQ AVTEPNIDRP HNRRIDERGT WNDVVFQTLH LREPKKWTAE 300
    TPNLYRLVVS LLDENGTHLE SEAYPVGFRK VEISEGQLKL NGKPLLIRGV 350
    NRHEHHPELG HVMTEEDMIR DICLMKQYNF NAVRTAHYPN HPRWYELCDQ 400
    YGLYVCDEAN IETHGMQPMS RLSSDPQWAH AYMSRYTQMV LRDKNHPSII 450
    IWSLGNESGH GSNHNAMYAW SKNFDPSRPV QYEGGGSNTT ATDIIAPMYA 500
    RVNTLIADEA VPKWPIKKWI SLPNETRPLI LCEYAHAMGN SLGSFDEYWA 550
    AFREFPRLQG GFIWDWVDQG LSQWDENGQH FWAYGGDFGD EINDRQFCIN 600
    GLIFPDRTVH PTLQEAKYCQ RMITVSLQEQ TQKACTLLVT NENLFRATDN 650
    EQLNWSLLEN GQVIQTGSLA LSVEADSQTR LEIALNFTPK AQAQYHLNTD 700
    ICLIEATSWA PAGHVVATEQ MALRNHAGLA IPTLRTQPAP KLTQDGHAIV 750
    VSSLDEKHQW RWDSQSGLLM EWNVDGKAQM LAAPQDNFFR APLDNDIGIS 800
    EVDNVDPNAW VCRWEMAGIG QWERHCVHCD SETLAHTVVV TTTFAYHFGG 850
    DVQAITQWTH TLSNDGEMLL DVDVTLADTL PPMPRIGLEL QLPLYQADTP 900
    ITWQGLGPFE NYPDRLAAAR FGLHTQTLAQ MHTPYIFPTD SGLRCGTQWL 950
    QVNELAISGD FQFSVSQYAQ QQLAEAKHTH DLLAQERIYL RLDHQHMGVG 1000
    GDDSWSPSVH KEFQLTEKHY RYQLRFKPAS R 1031
    Length:1,031
    Mass (Da):117,763
    Last modified:December 15, 2003 - v1
    Checksum:i3A3E6A9AD4BC5560
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000038 Genomic DNA. Translation: BAC96191.1.
    RefSeqiNP_936221.1. NC_005140.1.

    Genome annotation databases

    EnsemblBacteriaiBAC96191; BAC96191; BAC96191.
    GeneIDi2621483.
    KEGGivvy:VVA0165.
    PATRICi20173734. VBIVibVul40472_3382.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000038 Genomic DNA. Translation: BAC96191.1 .
    RefSeqi NP_936221.1. NC_005140.1.

    3D structure databases

    ProteinModelPortali Q7MG04.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196600.VVA0165.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC96191 ; BAC96191 ; BAC96191 .
    GeneIDi 2621483.
    KEGGi vvy:VVA0165.
    PATRICi 20173734. VBIVibVul40472_3382.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci VVUL196600:GJ9W-3552-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: YJ016.

    Entry informationi

    Entry nameiBGAL_VIBVY
    AccessioniPrimary (citable) accession number: Q7MG04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3