Q7MF46 (PHNX_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphonoacetaldehyde hydrolase
Short name=Phosphonatase
EC=3.11.1.1

Alternative name(s):
Phosphonoacetaldehyde phosphonohydrolase

Gene names

Name:	phnX
Ordered Locus Names:	VVA0474

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio vulnificus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity. HAMAP-Rule MF_01375
Catalytic activity	Phosphonoacetaldehyde + H2O = acetaldehyde + phosphate. HAMAP-Rule MF_01375
Cofactor	Binds 1 Mg2+ ion per subunit By similarity. HAMAP-Rule MF_01375
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01375
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PhnX family.
Sequence caution	The sequence BAC96500.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Schiff base
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphonoacetaldehyde hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Phosphonoacetaldehyde hydrolase HAMAP-Rule MF_01375		PRO_0000284606
Sites
	Active site	12	1	Nucleophile By similarity
	Active site	54	1	Schiff-base intermediate with substrate By similarity
	Metal binding	12	1	Magnesium By similarity
	Metal binding	14	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	188	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MF46 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: 820603F976FD7878	FASTA	271	29,617
	10 20 30 40 50 60 MNKSPIQAVI FDWAGTIVDF GSFAPTSIFV EAFKQGFDFE IDLEEAREPM GLGKWDHIQA 70 80 90 100 110 120 VGRIPAVDKR WNEKFGRSMI SEDIDAIYAA FMPLQKAKVA DHAEPILNAI EVVNGLKDKG 130 140 150 160 170 180 IKIGSCSGYP REVMDVLIPV AADYGYQPDY VVATDDLPQG GRPAPFMALK NVIELGVSDV 190 200 210 220 230 240 KACVKVDDSA PGIFEGHNAG MWTVGLLLSG NEAGLTFEEY QAADEATLEK AREKARAKLL 250 260 270 KSSPHYLIDT IADFPEVVAD IERRLAAGER P

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YJ016.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000038 Genomic DNA. Translation: BAC96500.1. Different initiation.
RefSeq	NP_936530.1. NC_005140.1.
3D structure databases
ProteinModelPortal	Q7MF46.
ModBase	Search...
Protein-protein interaction databases
STRING	196600.VVA0474.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC96500; BAC96500; BAC96500.
GeneID	2621241.
KEGG	vvy:VVA0474.
PATRIC	20174340. VBIVibVul40472_3677.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0637.
HOGENOM	HOG000217971.
KO	K05306.
OMA	EFGATWE.
ProtClustDB	PRK13478.
Enzyme and pathway databases
BioCyc	VVUL196600:GJ9W-3868-MONOMER.
Family and domain databases
Gene3D	1.10.150.240. 1 hit. 3.40.50.1000. 2 hits.
HAMAP	MF_01375. PhnX.
InterPro	IPR023214. HAD-like_dom. IPR006402. HAD-SF_hydro_IA_v3. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro. [Graphical view]
Pfam	PF13419. HAD_2. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNX_VIBVY

Accession

Primary (citable) accession number: Q7MF46

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	May 29, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program