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Q7ME85 (DDL_VIBVY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:VVA0785
OrganismVibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]
Taxonomic identifier196600 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177904

Regions

Domain122 – 328207ATP-grasp
Nucleotide binding152 – 20756ATP By similarity

Sites

Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2951Magnesium or manganese 1 By similarity
Metal binding2951Magnesium or manganese 2 By similarity
Metal binding2971Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7ME85 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 15AC38DC46D47880

FASTA33137,353
        10         20         30         40         50         60 
MMRNVLLLCG GGSSEHEISL LSSEYLQQQL GLIENVNVLK VEIKNEGWFD QKERLVYLDI 

        70         80         90        100        110        120 
HTKSVKSDEF NESIDIDFIV PCIHGFPGET GDIQSLFELA GIPYLGCGPE ASSNSFNKIT 

       130        140        150        160        170        180 
SKLWYDAIGI PNTPYLFLTE NNEESHQLSR EAFDKWGKLF VKAARQGSSV GCYSVTNIEQ 

       190        200        210        220        230        240 
LSDAIDKAFG FSHQVLVEKA VKPRELEVSA YEMNGQLHIS KPGEIIAPDG AFYSYDEKYS 

       250        260        270        280        290        300 
AGSHSITEVE AKNLTEQQLA TIQLCSEKVF RQMNLRHLSR IDFFLTSEGE IYLNEVNTFP 

       310        320        330 
GMTKISMFPK MLQHNGHKFH EFLADCIERS L 

« Hide

References

[1]"Comparative genome analysis of Vibrio vulnificus, a marine pathogen."
Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F.
Genome Res. 13:2577-2587(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YJ016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000038 Genomic DNA. Translation: BAC96811.1.
RefSeqNP_936841.1. NC_005140.1.

3D structure databases

ProteinModelPortalQ7ME85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196600.VVA0785.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC96811; BAC96811; BAC96811.
GeneID2621642.
KEGGvvy:VVA0785.
PATRIC20174942. VBIVibVul40472_3976.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycVVUL196600:GJ9W-4181-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_VIBVY
AccessionPrimary (citable) accession number: Q7ME85
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways