Reviewed, UniProtKB/Swiss-Prot Q7MCN7 (SYY1_VIBVY)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase 1
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 1
      Short name=TyrRS 1

Gene names

Name:	tyrS1
Ordered Locus Names:	VVA1349

Organism

Vibrio vulnificus (strain YJ016) [Complete proteome] [HAMAP]

Taxonomic identifier

196600 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Tyrosyl-tRNA synthetase 1 HAMAP MF_02006		PRO_0000234812
Regions
	Domain	355 – 419	65	S4 RNA-binding
	Motif	41 – 50	10	"HIGH" region HAMAP MF_02006
	Motif	233 – 237	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	36	1	Tyrosine By similarity
	Binding site	173	1	Tyrosine By similarity
	Binding site	177	1	Tyrosine By similarity
	Binding site	236	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MCN7-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 0AC47AC4DBA654F5	FASTA	422	47,162
	10 20 30 40 50 60 MNSQLLFDDL AQRGLIAQTT DLEQLIALFR QPQTLYCGFD PTAGSLHIGH LVPLIMLKRF 70 80 90 100 110 120 QDAGHQGIAL IGGATGMIGD PSFKASERSL NSAETVAAWV NALATQIQQL MTPHLTQPLV 130 140 150 160 170 180 MVNNADWMQS IGVIAFFRDI GKHFSVNAMI QRESVKQRLA RPDQGISFTE FSYSLLQSYD 190 200 210 220 230 240 FAQLNQTHQC ALQIGGNDQW GNIVSGIDLT RRLNGTTVHG LTLPLITKSD GTKFGKTEGG 250 260 270 280 290 300 AIWLDAAKTS PYMFYQFWLN CDDADVYRFL RYYTFLSVEQ IEQIEATDKA QVGKPSAQRI 310 320 330 340 350 360 LAEEMTRFVH GHAGLESAQR ISQALFSGQL NQLNLAELKQ LEQDGLPCRQ LAHVSDVVTL 370 380 390 400 410 420 LLETGLASSK RQAREWLENG AIRINGERWN EQTLAQTFAL YDQYYIVQRG KKQFAMVKLA QV

References

[1]

"Comparative genome analysis of Vibrio vulnificus, a marine pathogen." Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C., Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L., Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F. Genome Res. 13:2577-2587(2003) [PubMed: 14656965] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000038 Genomic DNA. Translation: BAC97375.1.
RefSeq	NP_937405.1.
3D structure databases
SMR	Q7MCN7. Positions 1-320, 6-419.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7MCN7.
Genome annotation databases
GeneID	2622211.
GenomeReviews	Gene locus VVA1349 in contig BA000038_GR.
KEGG	vvy:VVA1349.
NMPDR	fig|196600.1.peg.4676.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	TFYIGFD.
Enzyme and pathway databases
BioCyc	VVUL196600:VVA1349-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY1_VIBVY

Accession

Primary (citable) accession number: Q7MCN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)