Reviewed, UniProtKB/Swiss-Prot Q7MAX9 (PURA2_PHOLL)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase 2
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase 2
    AdSS 2
    AMPSase 2

Gene names

Name:	purA2
Ordered Locus Names:	plu4577

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Adenylosuccinate synthetase 2 HAMAP MF_00011		PRO_0000095208
Regions
	Nucleotide binding	13 – 19	7	GTP Potential
Sites
	Active site	141	1	By similarity
	Active site	148	1	By similarity
	Metal binding	14	1	Magnesium By similarity
	Metal binding	41	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MAX9-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: B798323C9425F2CA	FASTA	432	47,240
	10 20 30 40 50 60 MGKNVVVLGT QWGDEGKGKI VDLLTERAKY VVRYQGGHNA GHTLVINGEK TVLHLIPSGI 70 80 90 100 110 120 LRENVISIIA NGVVLAPDAL MKEMNALESR GIPVRERLLI SEACPLILPY HVALDNAREK 130 140 150 160 170 180 ARGAKAIGTT GRGIGPAYED KVARRGLRVG DLFDKEAFAV KLKEIIEYHN FQLVNYYKEP 190 200 210 220 230 240 AVDYQKTLDE IMAVADILTD MVVDVSDLLY KATQKGELVM FEGAQGTLLD IDHGTYPYVT 250 260 270 280 290 300 SSNTTAGGVA TGSGLGPCYV DYVLGIIKAY STRVGAGPFP TELFDETGGY LREKGQEFGA 310 320 330 340 350 360 TTGRSRRTGW LDIIAIRRAV QINSLSGFCM TKLDVLDGLK EVKICTGYRM PDGSVIETTP 370 380 390 400 410 420 LAADDWEGIE PVYETMPGWN ESTFGVKEHR KLPQAALNYI KRVEELTGVP VDIISTGPDR 430 SETIILRDPF DA

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571874 Genomic DNA. Translation: CAE16949.1.
RefSeq	NP_931741.1.
3D structure databases
SMR	Q7MAX9. Positions 2-432.
ModBase	Search...
Genome annotation databases
GeneID	2804601.
GenomeReviews	Gene locus plu4577 in contig BX470251_GR.
KEGG	plu:plu4577.
NMPDR	fig|243265.1.peg.4364.
Organism-specific databases
PhotoList	plu4577.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MAX9.
OMA	YVLGIIK.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU4577-MON.
BRENDA	6.3.4.4. 308689.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA2_PHOLL

Accession

Primary (citable) accession number: Q7MAX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)