Q7MAX3 (DUT_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	plu4867

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus › Photorhabdus luminescens › Photorhabdus luminescens subsp. laumondii

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_0000182891
Regions
	Region	71 – 73	3	Substrate binding By similarity
	Region	88 – 90	3	Substrate binding By similarity
Sites
	Binding site	84	1	Substrate By similarity
	Binding site	98	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MAX3 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: EECBF0C5609AD89A	FASTA	152	16,302
	10 20 30 40 50 60 MMKKIDVKIL DPRIGEEFPL PTYATPGSAG LDLRACLDNA VELAPGQTEL LPTGLAIHIG 70 80 90 100 110 120 DEQLAAVILP RSGLGHKHGV VLGNLVGLID SDYQGQLMVS VWNRGDKAFT IQPGERIAQI 130 140 150 VFVPVVQAEF NLVEDFETSE RGSGGFGHSG RQ

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571875 Genomic DNA. Translation: CAE17239.1.
RefSeq	NP_932021.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7MAX3.
SMR	Q7MAX3. Positions 1-139.
ModBase	Search...
Protein-protein interaction databases
STRING	243265.plu4867.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE17239; CAE17239; plu4867.
GeneID	2804914.
KEGG	plu:plu4867.
PATRIC	20514572. VBIPhoLum48522_5500.
Organism-specific databases
GenoList	plu4867.
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028968.
KO	K01520.
OMA	ILPRSGM.
ProtClustDB	PRK00601.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_PHOLL

Accession

Primary (citable) accession number: Q7MAX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program