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Q7MAR0 (GLYA_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine hydroxymethyltransferase

Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:WS0085
OrganismWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) [Complete proteome] [HAMAP]
Taxonomic identifier273121 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity. HAMAP-Rule MF_00051

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP-Rule MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1. HAMAP-Rule MF_00051

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00051

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00051.

Sequence similarities

Belongs to the SHMT family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
One-carbon metabolism
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycine biosynthetic process from serine

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglycine hydroxymethyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Serine hydroxymethyltransferase HAMAP-Rule MF_00051
PRO_0000113700

Regions

Region122 – 1243Substrate binding By similarity
Region350 – 3523Substrate binding By similarity

Sites

Binding site321Pyridoxal phosphate By similarity
Binding site521Pyridoxal phosphate By similarity
Binding site541Substrate By similarity
Binding site611Substrate By similarity
Binding site621Pyridoxal phosphate By similarity
Binding site961Pyridoxal phosphate By similarity
Binding site1181Substrate; via carbonyl oxygen By similarity
Binding site1721Pyridoxal phosphate By similarity
Binding site2001Pyridoxal phosphate By similarity
Binding site2251Pyridoxal phosphate By similarity
Binding site2321Pyridoxal phosphate By similarity
Binding site2581Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3581Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2261N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MAR0 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 0418DE3016CA94E9

FASTA41645,667
        10         20         30         40         50         60 
MNYALETNDK EIFDLIHEEL DRQNTHLEMI ASENFTFPAV MEAMGSVLTN KYAEGYPYKR 

        70         80         90        100        110        120 
YYGGCEFVDR VEEIAIERAK KLFGCGFANV QPHAGSQANV AVYNALLKPY DKILGMDLSH 

       130        140        150        160        170        180 
GGHLTHGAKV SVTGQTYQSF FYGVELDGYI NYDKVEEIAK IVKPQMIVCG FSAYARELDF 

       190        200        210        220        230        240 
KRFREIADSV GALLLGDVAH VAGLIVAGEY PNPFPHCHIV TTTTHKTLRG PRGGMILTND 

       250        260        270        280        290        300 
EEIAKKIDKA VFPGMQGGPL MHVIAAKAVG FGENLKPEWK EYAKQVKANA KVLAKVLMAR 

       310        320        330        340        350        360 
GYTLVSGGTD NHLILVSLLD KEFSGKDADR ALGEAGITVN KNTVPGETRS PFVTSGVRIG 

       370        380        390        400        410 
SAALTARGMR EKEFEFIATK IADVLDDVNN AAKHAEIKKE IAEFAKGFPV YTKAIF 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571657 Genomic DNA. Translation: CAE09253.1.
RefSeqNP_906353.1. NC_005090.1.

3D structure databases

ProteinModelPortalQ7MAR0.
SMRQ7MAR0. Positions 5-405.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273121.WS0085.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE09253; CAE09253; WS0085.
GeneID2555508.
KEGGwsu:WS0085.
PATRIC24037254. VBIWolSuc63014_0083.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0112.
HOGENOMHOG000239405.
KOK00600.
OMAFQYCDVV.
OrthoDBEOG6Z0QB2.

Enzyme and pathway databases

UniPathwayUPA00193.
UPA00288; UER01023.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00051. SHMT.
InterProIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERPTHR11680. PTHR11680. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_WOLSU
AccessionPrimary (citable) accession number: Q7MAR0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways