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Q7MAR0

- GLYA_WOLSU

UniProt

Q7MAR0 - GLYA_WOLSU

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Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

Pyridoxal phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321Pyridoxal phosphateUniRule annotation
Binding sitei52 – 521Pyridoxal phosphateUniRule annotation
Binding sitei54 – 541SubstrateUniRule annotation
Binding sitei61 – 611SubstrateUniRule annotation
Binding sitei62 – 621Pyridoxal phosphateUniRule annotation
Binding sitei96 – 961Pyridoxal phosphateUniRule annotation
Binding sitei118 – 1181Substrate; via carbonyl oxygenUniRule annotation
Binding sitei172 – 1721Pyridoxal phosphateUniRule annotation
Binding sitei200 – 2001Pyridoxal phosphateUniRule annotation
Binding sitei225 – 2251Pyridoxal phosphateUniRule annotation
Binding sitei232 – 2321Pyridoxal phosphateUniRule annotation
Binding sitei258 – 2581Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei358 – 3581Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, One-carbon metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
Short name:
SHMTUniRule annotation
Short name:
Serine methylaseUniRule annotation
Gene namesi
Name:glyAUniRule annotation
Ordered Locus Names:WS0085
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Serine hydroxymethyltransferasePRO_0000113700Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei226 – 2261N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS0085.

Structurei

3D structure databases

ProteinModelPortaliQ7MAR0.
SMRiQ7MAR0. Positions 5-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1243Substrate bindingUniRule annotation
Regioni350 – 3523Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the SHMT family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239405.
KOiK00600.
OMAiFQYCDVV.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MAR0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYALETNDK EIFDLIHEEL DRQNTHLEMI ASENFTFPAV MEAMGSVLTN
60 70 80 90 100
KYAEGYPYKR YYGGCEFVDR VEEIAIERAK KLFGCGFANV QPHAGSQANV
110 120 130 140 150
AVYNALLKPY DKILGMDLSH GGHLTHGAKV SVTGQTYQSF FYGVELDGYI
160 170 180 190 200
NYDKVEEIAK IVKPQMIVCG FSAYARELDF KRFREIADSV GALLLGDVAH
210 220 230 240 250
VAGLIVAGEY PNPFPHCHIV TTTTHKTLRG PRGGMILTND EEIAKKIDKA
260 270 280 290 300
VFPGMQGGPL MHVIAAKAVG FGENLKPEWK EYAKQVKANA KVLAKVLMAR
310 320 330 340 350
GYTLVSGGTD NHLILVSLLD KEFSGKDADR ALGEAGITVN KNTVPGETRS
360 370 380 390 400
PFVTSGVRIG SAALTARGMR EKEFEFIATK IADVLDDVNN AAKHAEIKKE
410
IAEFAKGFPV YTKAIF
Length:416
Mass (Da):45,667
Last modified:December 15, 2003 - v1
Checksum:i0418DE3016CA94E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571657 Genomic DNA. Translation: CAE09253.1.
RefSeqiNP_906353.1. NC_005090.1.
WP_011138053.1. NC_005090.1.

Genome annotation databases

EnsemblBacteriaiCAE09253; CAE09253; WS0085.
GeneIDi2555508.
KEGGiwsu:WS0085.
PATRICi24037254. VBIWolSuc63014_0083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571657 Genomic DNA. Translation: CAE09253.1 .
RefSeqi NP_906353.1. NC_005090.1.
WP_011138053.1. NC_005090.1.

3D structure databases

ProteinModelPortali Q7MAR0.
SMRi Q7MAR0. Positions 5-405.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273121.WS0085.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE09253 ; CAE09253 ; WS0085 .
GeneIDi 2555508.
KEGGi wsu:WS0085.
PATRICi 24037254. VBIWolSuc63014_0083.

Phylogenomic databases

eggNOGi COG0112.
HOGENOMi HOG000239405.
KOi K00600.
OMAi FQYCDVV.
OrthoDBi EOG6Z0QB2.

Enzyme and pathway databases

UniPathwayi UPA00193 .
UPA00288 ; UER01023 .

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00051. SHMT.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view ]
PANTHERi PTHR11680. PTHR11680. 1 hit.
Pfami PF00464. SHMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000412. SHMT. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00096. SHMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Entry informationi

Entry nameiGLYA_WOLSU
AccessioniPrimary (citable) accession number: Q7MAR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3