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Q7MAR0

- GLYA_WOLSU

UniProt

Q7MAR0 - GLYA_WOLSU

Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321Pyridoxal phosphateUniRule annotation
    Binding sitei52 – 521Pyridoxal phosphateUniRule annotation
    Binding sitei54 – 541SubstrateUniRule annotation
    Binding sitei61 – 611SubstrateUniRule annotation
    Binding sitei62 – 621Pyridoxal phosphateUniRule annotation
    Binding sitei96 – 961Pyridoxal phosphateUniRule annotation
    Binding sitei118 – 1181Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei172 – 1721Pyridoxal phosphateUniRule annotation
    Binding sitei200 – 2001Pyridoxal phosphateUniRule annotation
    Binding sitei225 – 2251Pyridoxal phosphateUniRule annotation
    Binding sitei232 – 2321Pyridoxal phosphateUniRule annotation
    Binding sitei258 – 2581Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei358 – 3581Pyridoxal phosphateUniRule annotation

    GO - Molecular functioni

    1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, One-carbon metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00193.
    UPA00288; UER01023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
    Short name:
    SHMTUniRule annotation
    Short name:
    Serine methylaseUniRule annotation
    Gene namesi
    Name:glyAUniRule annotation
    Ordered Locus Names:WS0085
    OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
    Taxonomic identifieri273121 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
    ProteomesiUP000000422: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Serine hydroxymethyltransferasePRO_0000113700Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei226 – 2261N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273121.WS0085.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7MAR0.
    SMRiQ7MAR0. Positions 5-405.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni122 – 1243Substrate bindingUniRule annotation
    Regioni350 – 3523Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the SHMT family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0112.
    HOGENOMiHOG000239405.
    KOiK00600.
    OMAiFQYCDVV.
    OrthoDBiEOG6Z0QB2.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00051. SHMT.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view]
    PANTHERiPTHR11680. PTHR11680. 1 hit.
    PfamiPF00464. SHMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000412. SHMT. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00096. SHMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7MAR0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYALETNDK EIFDLIHEEL DRQNTHLEMI ASENFTFPAV MEAMGSVLTN    50
    KYAEGYPYKR YYGGCEFVDR VEEIAIERAK KLFGCGFANV QPHAGSQANV 100
    AVYNALLKPY DKILGMDLSH GGHLTHGAKV SVTGQTYQSF FYGVELDGYI 150
    NYDKVEEIAK IVKPQMIVCG FSAYARELDF KRFREIADSV GALLLGDVAH 200
    VAGLIVAGEY PNPFPHCHIV TTTTHKTLRG PRGGMILTND EEIAKKIDKA 250
    VFPGMQGGPL MHVIAAKAVG FGENLKPEWK EYAKQVKANA KVLAKVLMAR 300
    GYTLVSGGTD NHLILVSLLD KEFSGKDADR ALGEAGITVN KNTVPGETRS 350
    PFVTSGVRIG SAALTARGMR EKEFEFIATK IADVLDDVNN AAKHAEIKKE 400
    IAEFAKGFPV YTKAIF 416
    Length:416
    Mass (Da):45,667
    Last modified:December 15, 2003 - v1
    Checksum:i0418DE3016CA94E9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571657 Genomic DNA. Translation: CAE09253.1.
    RefSeqiNP_906353.1. NC_005090.1.
    WP_011138053.1. NC_005090.1.

    Genome annotation databases

    EnsemblBacteriaiCAE09253; CAE09253; WS0085.
    GeneIDi2555508.
    KEGGiwsu:WS0085.
    PATRICi24037254. VBIWolSuc63014_0083.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571657 Genomic DNA. Translation: CAE09253.1 .
    RefSeqi NP_906353.1. NC_005090.1.
    WP_011138053.1. NC_005090.1.

    3D structure databases

    ProteinModelPortali Q7MAR0.
    SMRi Q7MAR0. Positions 5-405.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273121.WS0085.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE09253 ; CAE09253 ; WS0085 .
    GeneIDi 2555508.
    KEGGi wsu:WS0085.
    PATRICi 24037254. VBIWolSuc63014_0083.

    Phylogenomic databases

    eggNOGi COG0112.
    HOGENOMi HOG000239405.
    KOi K00600.
    OMAi FQYCDVV.
    OrthoDBi EOG6Z0QB2.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    UPA00288 ; UER01023 .

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00051. SHMT.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR001085. Ser_HO-MeTrfase.
    IPR019798. Ser_HO-MeTrfase_PLP_BS.
    [Graphical view ]
    PANTHERi PTHR11680. PTHR11680. 1 hit.
    Pfami PF00464. SHMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000412. SHMT. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00096. SHMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

    Entry informationi

    Entry nameiGLYA_WOLSU
    AccessioniPrimary (citable) accession number: Q7MAR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3