Reviewed, UniProtKB/Swiss-Prot Q7MAQ8 (HIS2_WOLSU)

Names and origin

Protein names

Recommended name:
    Histidine biosynthesis bifunctional protein hisIE
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosyl-AMP cyclohydrolase
                Short name=PRA-CH
              EC=3.5.4.19
    2- Recommended name:
            Phosphoribosyl-ATP pyrophosphatase
                Short name=PRA-PH
              EC=3.6.1.31

Gene names

Name:	hisI
Synonyms:	hisIE
Ordered Locus Names:	WS0090

Organism

Wolinella succinogenes [Complete proteome] [HAMAP]

Taxonomic identifier

844 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-ATP + H2O = 1-(5-phosphoribosyl)-AMP + diphosphate. HAMAP MF_01019 1-(5-phosphoribosyl)-AMP + H2O = 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide. HAMAP MF_01019
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9. HAMAP MF_01019 Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the PRA-CH family. In the C-terminal section; belongs to the PRA-PH family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosyl-AMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosyl-ATP diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Histidine biosynthesis bifunctional protein hisIE HAMAP MF_01019		PRO_0000136449
Regions
	Region	1 – 127	127	Phosphoribosyl-AMP cyclohydrolase HAMAP MF_01019
	Region	128 – 229	102	Phosphoribosyl-ATP pyrophosphohydrolase HAMAP MF_01019

Sequences

	Sequence		Length	Mass (Da)
	Q7MAQ8-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 862E1C69B74ABD83	FASTA	229	25,439
	10 20 30 40 50 60 MNTLLDGIDW EKHPLLPAIV QERGSGEVLM LAYMNQEALN LTLSTQVAHY FSRSKGRIWK 70 80 90 100 110 120 KGESSGHIQK IHEIFLDCDS DTILLQVEQV GVACHTGRKS CFFQKVELDS SLSLTSEAPD 130 140 150 160 170 180 TSALYGVVDR LYHELLARQG ADPQSSYTAK LFSKGENTIG KKIVEEAAEL SFAIKDSSES 190 200 210 220 EIVYEAADLL YHALVGLAFR GIHPDKIKQE LQRRQGVSGI AEKNSRKDS

References

[1]

"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed: 14500908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSMZ 1740.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571657 Genomic DNA. Translation: CAE09258.1.
RefSeq	NP_906358.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2555524.
GenomeReviews	Gene locus WS0090 in contig BX571656_GR.
KEGG	wsu:WS0090.
NMPDR	fig|273121.1.peg.85.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7MAQ8.
OMA	VHYWSRS.
Enzyme and pathway databases
BioCyc	WSUC273121:WS0090-MON.
BRENDA	3.5.4.19. 191624. 3.6.1.31. 191624.
Family and domain databases
HAMAP	MF_01019. [Tree]
InterPro	IPR002496. PRA_CycHdrlase. IPR008179. PRib-ATP_pyrophosphohydrolase. [Graphical view]
Pfam	PF01502. PRA-CH. 1 hit. PF01503. PRA-PH. 1 hit. [Graphical view]
ProDom	PD002610. PRA_cyclohydro. 1 hit. PD002611. Pra_PH/CH. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03188. histidine_hisI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS2_WOLSU

Accession

Primary (citable) accession number: Q7MAQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)