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Q7MAL0

- SYI_WOLSU

UniProt

Q7MAL0 - SYI_WOLSU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:WS0176
    OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
    Taxonomic identifieri273121 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
    ProteomesiUP000000422: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 915915Isoleucine--tRNA ligasePRO_0000098503Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273121.WS0176.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7MAL0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7MAL0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETIELP STTFPMRASL PENEPKRYEK WREEKVYAKM QKNREGAKEC    50
    FNLHDGPPYA NGHLHIGHAL NKILKDTIVK YHYFQGKKVS YVPGWDCHGL 100
    PIEQQVEKKL GKEKKDSLPK EKIRELCRAH AAEFIAIQKS EFLELGVVGD 150
    FENPYKTMDF PFEAAIYRAL CQIAQKGLLA ERSKPVYWSW ACKTALAEAE 200
    VEYQDKVSDS VYVAFPLQKE ALERLGVKRA VLVIWTTTPW TLPANVAVAL 250
    RPKELYVLMS DGKVVAKNLV EKLTALGVVG GSILQEFDSS TLENLLAQNP 300
    LNGRDSRIVL GEHVEVSDGT GCVHTAPGHG EDDYRVGLRY NLPVLMPVDD 350
    EGRFDETIQR ESLLPEADSF VGMHIFEAQK RILGLLGDAL LKHTQITHSY 400
    PHCWRSHEPV IFRATKQWFV LMDESIQGVG KSLREIALEE IQKTCFYPDH 450
    GIKRLGSMIE NRPDWCISRQ RDWGVPIAFF RDRQSGEMIF DAEVLEHIAG 500
    IFEQEGCDAW WSRDNAYLLP ASWKNRADSL EKIHHILDVW FDSGSTWKAV 550
    LLSSAYEAGN YPASMYLEGS DQHRGWFQSS LLVSCAINHH APYQSILTHG 600
    FTVDEKGEKM SKSKGNVIAP KDVLKEFGSE ILRLWVASSD YQSDLKISQN 650
    ILKQVAENYR KIRNTIRFLL ANTNDLERLS PLEDLSEIDR WFMQEARVAL 700
    EETNALFGRY DFSKGLQEIN YFITNALSGI YLDLCKDSLY CDGKNSKTRR 750
    ASQSAMAYTL RALLATLAPV LTYTVDEALE HAGEVLKGGA QSVFDLVYEP 800
    LPLVEAPKVD FVKLEELRAK FFEVVDGLKK EKRLKNTLEV NLLLPPKEED 850
    FGGLAKWMMV SEVLKEAKGE ELASFELAGL TYRLLRATLH RCPRCWQFVS 900
    PKEETLCERC AGAIG 915
    Length:915
    Mass (Da):103,964
    Last modified:December 15, 2003 - v1
    Checksum:i0BE8F67A2A9F137F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571657 Genomic DNA. Translation: CAE09339.1.
    RefSeqiNP_906439.1. NC_005090.1.
    WP_011138139.1. NC_005090.1.

    Genome annotation databases

    EnsemblBacteriaiCAE09339; CAE09339; WS0176.
    GeneIDi2555472.
    KEGGiwsu:WS0176.
    PATRICi24037460. VBIWolSuc63014_0172.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571657 Genomic DNA. Translation: CAE09339.1 .
    RefSeqi NP_906439.1. NC_005090.1.
    WP_011138139.1. NC_005090.1.

    3D structure databases

    ProteinModelPortali Q7MAL0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273121.WS0176.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE09339 ; CAE09339 ; WS0176 .
    GeneIDi 2555472.
    KEGGi wsu:WS0176.
    PATRICi 24037460. VBIWolSuc63014_0172.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

    Entry informationi

    Entry nameiSYI_WOLSU
    AccessioniPrimary (citable) accession number: Q7MAL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3