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Q7MAL0

- SYI_WOLSU

UniProt

Q7MAL0 - SYI_WOLSU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:WS0176
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 915915Isoleucine--tRNA ligasePRO_0000098503Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS0176.

Structurei

3D structure databases

ProteinModelPortaliQ7MAL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7MAL0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKETIELP STTFPMRASL PENEPKRYEK WREEKVYAKM QKNREGAKEC
60 70 80 90 100
FNLHDGPPYA NGHLHIGHAL NKILKDTIVK YHYFQGKKVS YVPGWDCHGL
110 120 130 140 150
PIEQQVEKKL GKEKKDSLPK EKIRELCRAH AAEFIAIQKS EFLELGVVGD
160 170 180 190 200
FENPYKTMDF PFEAAIYRAL CQIAQKGLLA ERSKPVYWSW ACKTALAEAE
210 220 230 240 250
VEYQDKVSDS VYVAFPLQKE ALERLGVKRA VLVIWTTTPW TLPANVAVAL
260 270 280 290 300
RPKELYVLMS DGKVVAKNLV EKLTALGVVG GSILQEFDSS TLENLLAQNP
310 320 330 340 350
LNGRDSRIVL GEHVEVSDGT GCVHTAPGHG EDDYRVGLRY NLPVLMPVDD
360 370 380 390 400
EGRFDETIQR ESLLPEADSF VGMHIFEAQK RILGLLGDAL LKHTQITHSY
410 420 430 440 450
PHCWRSHEPV IFRATKQWFV LMDESIQGVG KSLREIALEE IQKTCFYPDH
460 470 480 490 500
GIKRLGSMIE NRPDWCISRQ RDWGVPIAFF RDRQSGEMIF DAEVLEHIAG
510 520 530 540 550
IFEQEGCDAW WSRDNAYLLP ASWKNRADSL EKIHHILDVW FDSGSTWKAV
560 570 580 590 600
LLSSAYEAGN YPASMYLEGS DQHRGWFQSS LLVSCAINHH APYQSILTHG
610 620 630 640 650
FTVDEKGEKM SKSKGNVIAP KDVLKEFGSE ILRLWVASSD YQSDLKISQN
660 670 680 690 700
ILKQVAENYR KIRNTIRFLL ANTNDLERLS PLEDLSEIDR WFMQEARVAL
710 720 730 740 750
EETNALFGRY DFSKGLQEIN YFITNALSGI YLDLCKDSLY CDGKNSKTRR
760 770 780 790 800
ASQSAMAYTL RALLATLAPV LTYTVDEALE HAGEVLKGGA QSVFDLVYEP
810 820 830 840 850
LPLVEAPKVD FVKLEELRAK FFEVVDGLKK EKRLKNTLEV NLLLPPKEED
860 870 880 890 900
FGGLAKWMMV SEVLKEAKGE ELASFELAGL TYRLLRATLH RCPRCWQFVS
910
PKEETLCERC AGAIG
Length:915
Mass (Da):103,964
Last modified:December 15, 2003 - v1
Checksum:i0BE8F67A2A9F137F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571657 Genomic DNA. Translation: CAE09339.1.
RefSeqiNP_906439.1. NC_005090.1.
WP_011138139.1. NC_005090.1.

Genome annotation databases

EnsemblBacteriaiCAE09339; CAE09339; WS0176.
GeneIDi2555472.
KEGGiwsu:WS0176.
PATRICi24037460. VBIWolSuc63014_0172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571657 Genomic DNA. Translation: CAE09339.1 .
RefSeqi NP_906439.1. NC_005090.1.
WP_011138139.1. NC_005090.1.

3D structure databases

ProteinModelPortali Q7MAL0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273121.WS0176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE09339 ; CAE09339 ; WS0176 .
GeneIDi 2555472.
KEGGi wsu:WS0176.
PATRICi 24037460. VBIWolSuc63014_0172.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Entry informationi

Entry nameiSYI_WOLSU
AccessioniPrimary (citable) accession number: Q7MAL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3