Q7MAE0 (SYW_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	WS0311

Organism

Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) [Complete proteome] [HAMAP]

Taxonomic identifier

273121 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella › Wolinella succinogenes

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00140
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00140.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Tryptophan--tRNA ligase HAMAP-Rule MF_00140		PRO_0000136711
Regions
	Motif	12 – 20	9	"HIGH" region HAMAP-Rule MF_00140
	Motif	195 – 199	5	"KMSKS" region HAMAP-Rule MF_00140
Sites
	Binding site	198	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MAE0 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 9E27F479E4983586	FASTA	328	37,038
	10 20 30 40 50 60 MIKKRVFSGI QPTGNIHLGN YLGAVKNWVD SQDEFDNIFC IVNSHAITIK QDPALLRAKT 70 80 90 100 110 120 YELAAVLLAC GIDMKKSKLF IQSEVDEHAA LAWILDCNIP MGDMSRMTQF KDKSQKNPKN 130 140 150 160 170 180 INIGLFNYPA LMAADILLYQ TDFVPVGEDQ KQHLELTRDV AERFNRDYGE TFKIPEPMIP 190 200 210 220 230 240 KMGARIMGLD DPTKKMSKSE GKPNHGIALL DTPDEILKKF KKATTDSENL VRFNPEQIGI 250 260 270 280 290 300 FNLLTIYQIF TGYSQEVIEA EFEGKGYGIF KVAVAEAVIE GLKPIQERYA KLTKEQGYLE 310 320 EVLSHGASEV RKIAQKTYKE VKERVGLI

References

[1]

"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571657 Genomic DNA. Translation: CAE09462.1.
RefSeq	NP_906562.1. NC_005090.1.
3D structure databases
ProteinModelPortal	Q7MAE0.
SMR	Q7MAE0. Positions 4-328.
ModBase	Search...
Protein-protein interaction databases
STRING	273121.WS0311.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE09462; CAE09462; WS0311.
GeneID	2554724.
KEGG	wsu:WS0311.
PATRIC	24037714. VBIWolSuc63014_0298.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059940.
KO	K01867.
OMA	HDCLYCV.
ProtClustDB	PRK00927.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_WOLSU

Accession

Primary (citable) accession number: Q7MAE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program