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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135L-tryptophanUniRule annotation1
Binding sitei186ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 13ATPUniRule annotation3
Nucleotide bindingi19 – 20ATPUniRule annotation2
Nucleotide bindingi147 – 149ATPUniRule annotation3
Nucleotide bindingi195 – 199ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciWSUC273121:G1GTD-306-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:WS0311
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
Proteomesi
  • UP000000422 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367111 – 328Tryptophan--tRNA ligaseAdd BLAST328

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS0311

Structurei

3D structure databases

ProteinModelPortaliQ7MAE0
SMRiQ7MAE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 20"HIGH" regionUniRule annotation9
Motifi195 – 199"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q7MAE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKRVFSGI QPTGNIHLGN YLGAVKNWVD SQDEFDNIFC IVNSHAITIK
60 70 80 90 100
QDPALLRAKT YELAAVLLAC GIDMKKSKLF IQSEVDEHAA LAWILDCNIP
110 120 130 140 150
MGDMSRMTQF KDKSQKNPKN INIGLFNYPA LMAADILLYQ TDFVPVGEDQ
160 170 180 190 200
KQHLELTRDV AERFNRDYGE TFKIPEPMIP KMGARIMGLD DPTKKMSKSE
210 220 230 240 250
GKPNHGIALL DTPDEILKKF KKATTDSENL VRFNPEQIGI FNLLTIYQIF
260 270 280 290 300
TGYSQEVIEA EFEGKGYGIF KVAVAEAVIE GLKPIQERYA KLTKEQGYLE
310 320
EVLSHGASEV RKIAQKTYKE VKERVGLI
Length:328
Mass (Da):37,038
Last modified:December 15, 2003 - v1
Checksum:i9E27F479E4983586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571657 Genomic DNA Translation: CAE09462.1
RefSeqiWP_011138263.1, NC_005090.1

Genome annotation databases

EnsemblBacteriaiCAE09462; CAE09462; WS0311
KEGGiwsu:WS0311

Similar proteinsi

Entry informationi

Entry nameiSYW_WOLSU
AccessioniPrimary (citable) accession number: Q7MAE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health