Q7MAD7 (PYRE_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	WS0315

Organism

Wolinella succinogenes [Complete proteome] [HAMAP]

Taxonomic identifier

844 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110769
Regions
	Region	116 – 124	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	120	1	Orotate By similarity
	Binding site	148	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MAD7 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: E7152B02AE7C9025	FASTA	205	22,195
	10 20 30 40 50 60 MSRLNIEQVY KEAQALLEGH FLLSSGNHSN FYLQSAKVLE NPKVAESLAK ALAEEIRAFG 70 80 90 100 110 120 LTIDTVCSPA LGGVLAGYEL ARALGVRFIF TERVEGKMTL RRGFEVASGE KILICEDIIT 130 140 150 160 170 180 TGGSAVESAM EVERLGAQVV GYAALANRGF CQRVGSSLER KPNAKLPPHL PFFALDDFVF 190 200 DLYSPQDCPL CKTGSKPIKP GSRGN

References

[1]

"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed: 14500908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSMZ 1740.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571657 Genomic DNA. Translation: CAE09466.1.
RefSeq	NP_906566.1. NC_005090.1.
3D structure databases
ProteinModelPortal	Q7MAD7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2554716.
GenomeReviews	Gene locus WS0315 in contig BX571656_GR.
KEGG	wsu:WS0315.
NMPDR	fig|273121.1.peg.293.
PATRIC	24037722. VBIWolSuc63014_0302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	WSUC273121:WS0315-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_WOLSU

Accession

Primary (citable) accession number: Q7MAD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program