Q7MAC6 (BIOD_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	WS0335

Organism

Wolinella succinogenes [Complete proteome] [HAMAP]

Taxonomic identifier

844 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302553
Regions
	Nucleotide binding	115 – 118	4	ATP By similarity
Sites
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	53	1	Magnesium 2 By similarity
	Metal binding	115	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7MAC6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 70DCF1CD5CEB69A7	FASTA	216	24,414
	10 20 30 40 50 60 MKPLFISATG TGVGKSYTTQ KLLQSLHQSG RKPLALKPIE TGANPTPEDA LLHLQLMQKL 70 80 90 100 110 120 QLDQGLSLEE ICLERYKLPA SPWVSAKKEG RCVDVDHLLR RLLEFQKRSD PLLIEGAGGL 130 140 150 160 170 180 LVPLLKEYFM IDLAEALEAH MVLVISGKLG GINEALLSLE ALQHRQMSYT LVLNLWEEEK 190 200 210 RSFEEISAPY WKERPERLFR LDSELEELTQ TLLSAI

References

[1]

"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed: 14500908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSMZ 1740.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571657 Genomic DNA. Translation: CAE09485.1.
RefSeq	NP_906585.1. NC_005090.1.
3D structure databases
ProteinModelPortal	Q7MAC6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2554628.
GenomeReviews	Gene locus WS0335 in contig BX571656_GR.
KEGG	wsu:WS0335.
NMPDR	fig|273121.1.peg.312.
PATRIC	24037768. VBIWolSuc63014_0321.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	IDTGIGK.
Enzyme and pathway databases
BioCyc	WSUC273121:WS0335-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_WOLSU

Accession

Primary (citable) accession number: Q7MAC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program