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Q7MA27 (PROB_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:WS0506
OrganismWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) [Complete proteome] [HAMAP]
Taxonomic identifier273121 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_0000109756

Regions

Nucleotide binding167 – 1682ATP By similarity
Nucleotide binding209 – 2157ATP By similarity

Sites

Binding site71ATP By similarity
Binding site461Substrate By similarity
Binding site1311Substrate By similarity
Binding site1471Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7MA27 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: B4D522CCEE8CA26B

FASTA26128,707
        10         20         30         40         50         60 
MRRIVIKVGS ALLSRGHEMA MDRMESLCRF IAELKQKGDE VILVTSAAVA AGYTALALDK 

        70         80         90        100        110        120 
RQLPNRQALA AIGQPLLMNL YHQTLAPLGI LPAQMLLSAY DFDSRKRTEN ARNTIEVLLS 

       130        140        150        160        170        180 
HGVLPIINEN DAVATKELDM LAVFGDNDRL SAHVAHYFSA EILLILSDID GYYDKNPCEF 

       190        200        210        220        230        240 
PDAKIRRVVN FLTPEELEAE VSPNNAFATG GIVTKLQAAD FLMKRGQKMF LANGFNLGDA 

       250        260 
RSFLLEGIHC QGTLFVQGGE S 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571658 Genomic DNA. Translation: CAE09643.1.
RefSeqNP_906743.1. NC_005090.1.

3D structure databases

ProteinModelPortalQ7MA27.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273121.WS0506.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE09643; CAE09643; WS0506.
GeneID2554050.
KEGGwsu:WS0506.
PATRIC24038104. VBIWolSuc63014_0483.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246368.
KOK00931.
OMAALIICSD.
OrthoDBEOG6PGK7G.
ProtClustDBPRK12314.

Enzyme and pathway databases

UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. SSF53633. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_WOLSU
AccessionPrimary (citable) accession number: Q7MA27
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways