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Q7M962 (NAPA_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:WS1178
OrganismWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) [Complete proteome] [HAMAP]
Taxonomic identifier273121 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella

Protein attributes

Sequence length928 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence caution

The sequence CAE10263.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333Tat-type signal Potential
Chain34 – 928895Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000046014

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7M962 [UniParc].

Last modified February 7, 2006. Version 2.
Checksum: 9D5A8D055AA6D451

FASTA928104,968
        10         20         30         40         50         60 
MAFSRREFLK SAAAASAASA VGMSVPSQLL AQAQEGEKGW RWDKSVCRFC GTGCGIMVAT 

        70         80         90        100        110        120 
KNDQIVAVKG DPAAPVNRGL NCIKGYFNAK IMYGADRLTD PLLRVNEKGE FDKQGKFKPV 

       130        140        150        160        170        180 
SWKKAFDVME AQFKRAYNEL GPTGIGVFGS GQYTIQEGYM AAKLIKGGFR SNNLDPNARH 

       190        200        210        220        230        240 
CMASAVAAFM ETFGIDEPAG CYDDIELTDT IITWGANMAE MHPILWARVT DKKLSNPDKV 

       250        260        270        280        290        300 
KVINLSTYTN RTSDLADIEI IFTPQTDLAI WNYIAREIVY NHPESIDMEF VKNHCIFTTG 

       310        320        330        340        350        360 
FTDIGYGMRT DIKHAKYDPK ELDIAAKERS KVLSEAEGVT LRYLGMKAGD VMEMKHNATA 

       370        380        390        400        410        420 
GNHWEITFED FKKALEPYTL DFVAKLAKGN SEESLESFKE KLQKLANLYI EKERKVVSFW 

       430        440        450        460        470        480 
TMGMNQHTRG TWVNEQSYMV HFLLGKQAKP GSGAFSLTGQ PSACGTAREV GTFSHRLPAD 

       490        500        510        520        530        540 
MVVANPKHRA VSEKIWKLPE GTLNPKMGAH YMNIMRDLED GKIKFAWIQV NNPWQNTANA 

       550        560        570        580        590        600 
NHWIKAAREM DNFIVCSDAY PGISAKVADL ILPTAMIYEK WGAYGNAERR TQHWRQQVVP 

       610        620        630        640        650        660 
VGNAMPDVWQ MAEFSKRFKL KEVWGAKKID DKLTLPDVLE KAKAMGYSPE DTLFEVLFAN 

       670        680        690        700        710        720 
SEAKSFKAKD PIGEGFENTE VFGDRRNVAG SDGEVFKGYG FFIHKYLWEE YRRFGNGHGH 

       730        740        750        760        770        780 
DLADFDTYHK VRGLKWPVVD GKETQWRFNA KYDPYARKAG SGEFAFYGDA MKELPRGDLL 

       790        800        810        820        830        840 
SPKTEEKFKL TNKAKIFFRP YMDPPEMPSS EYPLWLCTGR VLEHWHSGTM TMRVPELYRA 

       850        860        870        880        890        900 
VPEALCYMHP EDAKKLGVKQ NEAVWVESRR GKVKARVDTR GRNRTPLGLV YVPWFDEKVY 

       910        920 
INKVCLDATC PISKQTDFKK CAVKVYKA 

« Hide

References

« Hide 'large scale' references
[1]"Electron transport to periplasmic nitrate reductase (NapA) of Wolinella succinogenes is independent of a NapC protein."
Simon J., Saenger M., Schuster S.C., Gross R.
Mol. Microbiol. 49:69-79(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence and analysis of Wolinella succinogenes."
Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C.
Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ512686 Genomic DNA. Translation: CAD55547.1.
BX571660 Genomic DNA. Translation: CAE10263.1. Different initiation.
RefSeqNP_907363.1. NC_005090.1.

3D structure databases

ProteinModelPortalQ7M962.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273121.WS1178.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE10263; CAE10263; WS1178.
GeneID2554883.
KEGGwsu:WS1178.
PATRIC24039378. VBIWolSuc63014_1109.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OrthoDBEOG6CVV7G.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_WOLSU
AccessionPrimary (citable) accession number: Q7M962
Secondary accession number(s): Q8GBG1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families