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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BRENDAi1.7.99.4. 6642.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:WS1178
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signalUniRule annotationAdd
BLAST
Chaini34 – 928895Periplasmic nitrate reductasePRO_0000046014Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS1178.

Structurei

3D structure databases

ProteinModelPortaliQ7M962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7M962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSRREFLK SAAAASAASA VGMSVPSQLL AQAQEGEKGW RWDKSVCRFC
60 70 80 90 100
GTGCGIMVAT KNDQIVAVKG DPAAPVNRGL NCIKGYFNAK IMYGADRLTD
110 120 130 140 150
PLLRVNEKGE FDKQGKFKPV SWKKAFDVME AQFKRAYNEL GPTGIGVFGS
160 170 180 190 200
GQYTIQEGYM AAKLIKGGFR SNNLDPNARH CMASAVAAFM ETFGIDEPAG
210 220 230 240 250
CYDDIELTDT IITWGANMAE MHPILWARVT DKKLSNPDKV KVINLSTYTN
260 270 280 290 300
RTSDLADIEI IFTPQTDLAI WNYIAREIVY NHPESIDMEF VKNHCIFTTG
310 320 330 340 350
FTDIGYGMRT DIKHAKYDPK ELDIAAKERS KVLSEAEGVT LRYLGMKAGD
360 370 380 390 400
VMEMKHNATA GNHWEITFED FKKALEPYTL DFVAKLAKGN SEESLESFKE
410 420 430 440 450
KLQKLANLYI EKERKVVSFW TMGMNQHTRG TWVNEQSYMV HFLLGKQAKP
460 470 480 490 500
GSGAFSLTGQ PSACGTAREV GTFSHRLPAD MVVANPKHRA VSEKIWKLPE
510 520 530 540 550
GTLNPKMGAH YMNIMRDLED GKIKFAWIQV NNPWQNTANA NHWIKAAREM
560 570 580 590 600
DNFIVCSDAY PGISAKVADL ILPTAMIYEK WGAYGNAERR TQHWRQQVVP
610 620 630 640 650
VGNAMPDVWQ MAEFSKRFKL KEVWGAKKID DKLTLPDVLE KAKAMGYSPE
660 670 680 690 700
DTLFEVLFAN SEAKSFKAKD PIGEGFENTE VFGDRRNVAG SDGEVFKGYG
710 720 730 740 750
FFIHKYLWEE YRRFGNGHGH DLADFDTYHK VRGLKWPVVD GKETQWRFNA
760 770 780 790 800
KYDPYARKAG SGEFAFYGDA MKELPRGDLL SPKTEEKFKL TNKAKIFFRP
810 820 830 840 850
YMDPPEMPSS EYPLWLCTGR VLEHWHSGTM TMRVPELYRA VPEALCYMHP
860 870 880 890 900
EDAKKLGVKQ NEAVWVESRR GKVKARVDTR GRNRTPLGLV YVPWFDEKVY
910 920
INKVCLDATC PISKQTDFKK CAVKVYKA
Length:928
Mass (Da):104,968
Last modified:February 7, 2006 - v2
Checksum:i9D5A8D055AA6D451
GO

Sequence cautioni

The sequence CAE10263.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512686 Genomic DNA. Translation: CAD55547.1.
BX571660 Genomic DNA. Translation: CAE10263.1. Different initiation.
RefSeqiWP_041572131.1. NC_005090.1.

Genome annotation databases

EnsemblBacteriaiCAE10263; CAE10263; WS1178.
KEGGiwsu:WS1178.
PATRICi24039378. VBIWolSuc63014_1109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512686 Genomic DNA. Translation: CAD55547.1.
BX571660 Genomic DNA. Translation: CAE10263.1. Different initiation.
RefSeqiWP_041572131.1. NC_005090.1.

3D structure databases

ProteinModelPortaliQ7M962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273121.WS1178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE10263; CAE10263; WS1178.
KEGGiwsu:WS1178.
PATRICi24039378. VBIWolSuc63014_1109.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BRENDAi1.7.99.4. 6642.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Electron transport to periplasmic nitrate reductase (NapA) of Wolinella succinogenes is independent of a NapC protein."
    Simon J., Saenger M., Schuster S.C., Gross R.
    Mol. Microbiol. 49:69-79(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Entry informationi

Entry nameiNAPA_WOLSU
AccessioniPrimary (citable) accession number: Q7M962
Secondary accession number(s): Q8GBG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: July 22, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.