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Q7M962

- NAPA_WOLSU

UniProt

Q7M962 - NAPA_WOLSU

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Protein
Periplasmic nitrate reductase
Gene
napA, WS1178
Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:WS1178
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signal Reviewed prediction
Add
BLAST
Chaini34 – 928895Periplasmic nitrate reductaseUniRule annotation
PRO_0000046014Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS1178.

Structurei

3D structure databases

ProteinModelPortaliQ7M962.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7M962-1 [UniParc]FASTAAdd to Basket

« Hide

MAFSRREFLK SAAAASAASA VGMSVPSQLL AQAQEGEKGW RWDKSVCRFC    50
GTGCGIMVAT KNDQIVAVKG DPAAPVNRGL NCIKGYFNAK IMYGADRLTD 100
PLLRVNEKGE FDKQGKFKPV SWKKAFDVME AQFKRAYNEL GPTGIGVFGS 150
GQYTIQEGYM AAKLIKGGFR SNNLDPNARH CMASAVAAFM ETFGIDEPAG 200
CYDDIELTDT IITWGANMAE MHPILWARVT DKKLSNPDKV KVINLSTYTN 250
RTSDLADIEI IFTPQTDLAI WNYIAREIVY NHPESIDMEF VKNHCIFTTG 300
FTDIGYGMRT DIKHAKYDPK ELDIAAKERS KVLSEAEGVT LRYLGMKAGD 350
VMEMKHNATA GNHWEITFED FKKALEPYTL DFVAKLAKGN SEESLESFKE 400
KLQKLANLYI EKERKVVSFW TMGMNQHTRG TWVNEQSYMV HFLLGKQAKP 450
GSGAFSLTGQ PSACGTAREV GTFSHRLPAD MVVANPKHRA VSEKIWKLPE 500
GTLNPKMGAH YMNIMRDLED GKIKFAWIQV NNPWQNTANA NHWIKAAREM 550
DNFIVCSDAY PGISAKVADL ILPTAMIYEK WGAYGNAERR TQHWRQQVVP 600
VGNAMPDVWQ MAEFSKRFKL KEVWGAKKID DKLTLPDVLE KAKAMGYSPE 650
DTLFEVLFAN SEAKSFKAKD PIGEGFENTE VFGDRRNVAG SDGEVFKGYG 700
FFIHKYLWEE YRRFGNGHGH DLADFDTYHK VRGLKWPVVD GKETQWRFNA 750
KYDPYARKAG SGEFAFYGDA MKELPRGDLL SPKTEEKFKL TNKAKIFFRP 800
YMDPPEMPSS EYPLWLCTGR VLEHWHSGTM TMRVPELYRA VPEALCYMHP 850
EDAKKLGVKQ NEAVWVESRR GKVKARVDTR GRNRTPLGLV YVPWFDEKVY 900
INKVCLDATC PISKQTDFKK CAVKVYKA 928
Length:928
Mass (Da):104,968
Last modified:February 7, 2006 - v2
Checksum:i9D5A8D055AA6D451
GO

Sequence cautioni

The sequence CAE10263.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ512686 Genomic DNA. Translation: CAD55547.1.
BX571660 Genomic DNA. Translation: CAE10263.1. Different initiation.
RefSeqiNP_907363.1. NC_005090.1.

Genome annotation databases

EnsemblBacteriaiCAE10263; CAE10263; WS1178.
GeneIDi2554883.
KEGGiwsu:WS1178.
PATRICi24039378. VBIWolSuc63014_1109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ512686 Genomic DNA. Translation: CAD55547.1 .
BX571660 Genomic DNA. Translation: CAE10263.1 . Different initiation.
RefSeqi NP_907363.1. NC_005090.1.

3D structure databases

ProteinModelPortali Q7M962.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273121.WS1178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE10263 ; CAE10263 ; WS1178 .
GeneIDi 2554883.
KEGGi wsu:WS1178.
PATRICi 24039378. VBIWolSuc63014_1109.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OrthoDBi EOG6CVV7G.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Electron transport to periplasmic nitrate reductase (NapA) of Wolinella succinogenes is independent of a NapC protein."
    Simon J., Saenger M., Schuster S.C., Gross R.
    Mol. Microbiol. 49:69-79(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Entry informationi

Entry nameiNAPA_WOLSU
AccessioniPrimary (citable) accession number: Q7M962
Secondary accession number(s): Q8GBG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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