Q7M8U8 (NIFH_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogenase iron protein
EC=1.18.6.1

Alternative name(s):
Nitrogenase Fe protein
Nitrogenase component II
Nitrogenase reductase

Gene names

Name:	nifH
Ordered Locus Names:	WS1394

Organism

Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) [Complete proteome] [HAMAP]

Taxonomic identifier

273121 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella › Wolinella succinogenes

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein By similarity. HAMAP-Rule MF_00533
Catalytic activity	8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate. HAMAP-Rule MF_00533
Cofactor	Binds 1 4Fe-4S cluster per dimer By similarity.
Subunit structure	Homodimer By similarity.
Post-translational modification	The reversible ADP-ribosylation of Arg-115 inactivates the nitrogenase reductase and regulates nitrogenase activity By similarity. HAMAP-Rule MF_00533
Sequence similarities	Belongs to the NifH/BchL/ChlL family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	ADP-ribosylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Cellular_component	molybdenum-iron nitrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: HAMAP carbonyl sulfide nitrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Nitrogenase iron protein HAMAP-Rule MF_00533		PRO_1000211895
Regions
	Nucleotide binding	11 – 18	8	ATP Potential
Sites
	Metal binding	112	1	Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	147	1	Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Amino acid modifications
	Modified residue	115	1	ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7M8U8 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 6F372D2F5E201BA4	FASTA	303	32,832
	10 20 30 40 50 60 MAGLRQIAFY GKGGIGKSTT SQNTLAAMAY YFGKKILIVG CDPKADSTRL ILHEKAQSTI 70 80 90 100 110 120 MQLAAEVGTV EDLELEDVCK PGAGPFNPEQ PSPSGGWIKC AESGGPEPGV GCAGRGVITA 130 140 150 160 170 180 INFLEEEGAY SDELDFVSYD VLGDVVCGGF AMPIREGKAQ EIYIVMSGEM MAMYAANNIS 190 200 210 220 230 240 RGILKYASSG GVRLAGLICN ARMTDREYDL ANALATKLGT QMIHFVPRHN IVQHAELRRM 250 260 270 280 290 300 TVVEYAETST QAEEYKELAR KIVHNDLKVI PTPMNMDDLE SLLMEFGIAE QFDEENVGKK AEA

References

[1]

"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571660 Genomic DNA. Translation: CAE10460.1.
RefSeq	NP_907560.1. NC_005090.1.
3D structure databases
HSSP	HSSP built from PDB template 1G20 based on UniProtKB P00459.
ProteinModelPortal	Q7M8U8.
SMR	Q7M8U8. Positions 3-302.
ModBase	Search...
Protein-protein interaction databases
STRING	273121.WS1394.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE10460; CAE10460; WS1394.
GeneID	2555138.
KEGG	wsu:WS1394.
PATRIC	24039788. VBIWolSuc63014_1312.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1348.
HOGENOM	HOG000228826.
KO	K02588.
OMA	CTESGGP.
ProtClustDB	CLSK2760420.
Family and domain databases
HAMAP	MF_00533. NifH.
InterPro	IPR000392. Nitogenase_NifH/Reductase_ChlL. IPR005977. Nitrogenase_Fe_NifH. [Graphical view]
Pfam	PF00142. Fer4_NifH. 1 hit. [Graphical view]
PIRSF	PIRSF000363. Nitrogenase_iron. 1 hit.
PRINTS	PR00091. NITROGNASEII.
TIGRFAMs	TIGR01287. nifH. 1 hit.
PROSITE	PS00746. NIFH_FRXC_1. 1 hit. PS00692. NIFH_FRXC_2. 1 hit. PS51026. NIFH_FRXC_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIFH_WOLSU

Accession

Primary (citable) accession number: Q7M8U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program