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Q7M818

- THIC_WOLSU

UniProt

Q7M818 - THIC_WOLSU

Protein

Phosphomethylpyrimidine synthase

Gene

thiC

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.UniRule annotation

    Catalytic activityi

    5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801SubstrateUniRule annotation
    Binding sitei109 – 1091SubstrateUniRule annotation
    Binding sitei138 – 1381SubstrateUniRule annotation
    Binding sitei174 – 1741SubstrateUniRule annotation
    Binding sitei274 – 2741SubstrateUniRule annotation
    Metal bindingi278 – 2781ZincUniRule annotation
    Binding sitei301 – 3011SubstrateUniRule annotation
    Metal bindingi342 – 3421ZincUniRule annotation
    Metal bindingi422 – 4221Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi425 – 4251Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi430 – 4301Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. lyase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphomethylpyrimidine synthaseUniRule annotation (EC:4.1.99.17UniRule annotation)
    Alternative name(s):
    Hydroxymethylpyrimidine phosphate synthaseUniRule annotation
    Short name:
    HMP-P synthaseUniRule annotation
    Short name:
    HMP-phosphate synthaseUniRule annotation
    Short name:
    HMPP synthaseUniRule annotation
    Thiamine biosynthesis protein ThiCUniRule annotation
    Gene namesi
    Name:thiCUniRule annotation
    Ordered Locus Names:WS1941
    OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
    Taxonomic identifieri273121 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
    ProteomesiUP000000422: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 449449Phosphomethylpyrimidine synthasePRO_0000152850Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273121.WS1941.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni194 – 1963Substrate bindingUniRule annotation
    Regioni235 – 2384Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ThiC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0422.
    HOGENOMiHOG000224483.
    KOiK03147.
    OMAiHGKIENL.
    OrthoDBiEOG6NWBM5.

    Family and domain databases

    HAMAPiMF_00089. ThiC.
    InterProiIPR002817. ThiC.
    [Graphical view]
    PfamiPF01964. ThiC. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00190. thiC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7M818-1 [UniParc]FASTAAdd to Basket

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    MRQSWIEKRK NDSVRTQLHY ARAGIITEEM QAVANVEGLS EELIRSEVAR    50
    GRLVIPANIH HLSLSPMGIG VAARTKINAN IGSSSLASCI EEEIEKTLVS 100
    IKYGADTIMD LSTGGDLDAI RCAVIEHSSV PIGTVPIYQI LHDVQGDIKN 150
    LTIDSMLEVM ERQARQGVSY FTIHCGFLLE HLPKVAKRKM GIVSRGGSLM 200
    ASYMMHYHKQ NPFYEAFDEI LAICREYDVT LSLGDSLRPG CLADASDEAQ 250
    LAELKVLGEL TLRAWEKDVQ VMIEGPGHVP MNQIERNVKL QKELCHEAPF 300
    YVLGPLVTDI AAGYDHIASA IGAAIAAWKG VAMLCYVTPK EHLGLPNAKD 350
    VREGILAYKI AAHAADIARG RIGARDRDDA MSDARYAFDW NRQFELALDP 400
    DRAKEYHDES LPQEVFKEAK FCSMCGPKFC SYKISQEIVS KHASGESLT 449
    Length:449
    Mass (Da):49,657
    Last modified:December 15, 2003 - v1
    Checksum:iDB137D4EFE4DB6A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571662 Genomic DNA. Translation: CAE10947.1.
    RefSeqiNP_908047.1. NC_005090.1.
    WP_011139730.1. NC_005090.1.

    Genome annotation databases

    EnsemblBacteriaiCAE10947; CAE10947; WS1941.
    GeneIDi2554016.
    KEGGiwsu:WS1941.
    PATRICi24040822. VBIWolSuc63014_1823.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571662 Genomic DNA. Translation: CAE10947.1 .
    RefSeqi NP_908047.1. NC_005090.1.
    WP_011139730.1. NC_005090.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273121.WS1941.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE10947 ; CAE10947 ; WS1941 .
    GeneIDi 2554016.
    KEGGi wsu:WS1941.
    PATRICi 24040822. VBIWolSuc63014_1823.

    Phylogenomic databases

    eggNOGi COG0422.
    HOGENOMi HOG000224483.
    KOi K03147.
    OMAi HGKIENL.
    OrthoDBi EOG6NWBM5.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .

    Family and domain databases

    HAMAPi MF_00089. ThiC.
    InterProi IPR002817. ThiC.
    [Graphical view ]
    Pfami PF01964. ThiC. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00190. thiC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

    Entry informationi

    Entry nameiTHIC_WOLSU
    AccessioniPrimary (citable) accession number: Q7M818
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3