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Q7M818 (THIC_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphomethylpyrimidine synthase

EC=4.1.99.17
Alternative name(s):
Hydroxymethylpyrimidine phosphate synthase
Short name=HMP-P synthase
Short name=HMP-phosphate synthase
Short name=HMPP synthase
Thiamine biosynthesis protein ThiC
Gene names
Name:thiC
Ordered Locus Names:WS1941
OrganismWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) [Complete proteome] [HAMAP]
Taxonomic identifier273121 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction By similarity. HAMAP-Rule MF_00089

Catalytic activity

5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO. HAMAP-Rule MF_00089

Cofactor

Binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00089

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00089

Sequence similarities

Belongs to the ThiC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Phosphomethylpyrimidine synthase HAMAP-Rule MF_00089
PRO_0000152850

Regions

Region194 – 1963Substrate binding By similarity
Region235 – 2384Substrate binding By similarity

Sites

Metal binding2781Zinc By similarity
Metal binding3421Zinc By similarity
Metal binding4221Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding4251Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding4301Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Binding site801Substrate By similarity
Binding site1091Substrate By similarity
Binding site1381Substrate By similarity
Binding site1741Substrate By similarity
Binding site2741Substrate By similarity
Binding site3011Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7M818 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: DB137D4EFE4DB6A1

FASTA44949,657
        10         20         30         40         50         60 
MRQSWIEKRK NDSVRTQLHY ARAGIITEEM QAVANVEGLS EELIRSEVAR GRLVIPANIH 

        70         80         90        100        110        120 
HLSLSPMGIG VAARTKINAN IGSSSLASCI EEEIEKTLVS IKYGADTIMD LSTGGDLDAI 

       130        140        150        160        170        180 
RCAVIEHSSV PIGTVPIYQI LHDVQGDIKN LTIDSMLEVM ERQARQGVSY FTIHCGFLLE 

       190        200        210        220        230        240 
HLPKVAKRKM GIVSRGGSLM ASYMMHYHKQ NPFYEAFDEI LAICREYDVT LSLGDSLRPG 

       250        260        270        280        290        300 
CLADASDEAQ LAELKVLGEL TLRAWEKDVQ VMIEGPGHVP MNQIERNVKL QKELCHEAPF 

       310        320        330        340        350        360 
YVLGPLVTDI AAGYDHIASA IGAAIAAWKG VAMLCYVTPK EHLGLPNAKD VREGILAYKI 

       370        380        390        400        410        420 
AAHAADIARG RIGARDRDDA MSDARYAFDW NRQFELALDP DRAKEYHDES LPQEVFKEAK 

       430        440 
FCSMCGPKFC SYKISQEIVS KHASGESLT 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571662 Genomic DNA. Translation: CAE10947.1.
RefSeqNP_908047.1. NC_005090.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273121.WS1941.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE10947; CAE10947; WS1941.
GeneID2554016.
KEGGwsu:WS1941.
PATRIC24040822. VBIWolSuc63014_1823.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0422.
HOGENOMHOG000224483.
KOK03147.
OMAHGKIENL.
OrthoDBEOG6NWBM5.

Enzyme and pathway databases

UniPathwayUPA00060.

Family and domain databases

HAMAPMF_00089. ThiC.
InterProIPR002817. ThiC.
[Graphical view]
PfamPF01964. ThiC. 1 hit.
[Graphical view]
TIGRFAMsTIGR00190. thiC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIC_WOLSU
AccessionPrimary (citable) accession number: Q7M818
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways