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Q7M818

- THIC_WOLSU

UniProt

Q7M818 - THIC_WOLSU

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Protein

Phosphomethylpyrimidine synthase

Gene
thiC, WS1941
Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction By similarity.UniRule annotation

Catalytic activityi

5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801Substrate By similarity
Binding sitei109 – 1091Substrate By similarity
Binding sitei138 – 1381Substrate By similarity
Binding sitei174 – 1741Substrate By similarity
Binding sitei274 – 2741Substrate By similarity
Metal bindingi278 – 2781Zinc By similarity
Binding sitei301 – 3011Substrate By similarity
Metal bindingi342 – 3421Zinc By similarity
Metal bindingi422 – 4221Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi425 – 4251Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi430 – 4301Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. lyase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomethylpyrimidine synthase (EC:4.1.99.17)
Alternative name(s):
Hydroxymethylpyrimidine phosphate synthase
Short name:
HMP-P synthase
Short name:
HMP-phosphate synthase
Short name:
HMPP synthase
Thiamine biosynthesis protein ThiC
Gene namesi
Name:thiC
Ordered Locus Names:WS1941
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Phosphomethylpyrimidine synthaseUniRule annotationPRO_0000152850Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS1941.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1963Substrate binding By similarity
Regioni235 – 2384Substrate binding By similarity

Sequence similaritiesi

Belongs to the ThiC family.

Phylogenomic databases

eggNOGiCOG0422.
HOGENOMiHOG000224483.
KOiK03147.
OMAiHGKIENL.
OrthoDBiEOG6NWBM5.

Family and domain databases

HAMAPiMF_00089. ThiC.
InterProiIPR002817. ThiC.
[Graphical view]
PfamiPF01964. ThiC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00190. thiC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7M818-1 [UniParc]FASTAAdd to Basket

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MRQSWIEKRK NDSVRTQLHY ARAGIITEEM QAVANVEGLS EELIRSEVAR    50
GRLVIPANIH HLSLSPMGIG VAARTKINAN IGSSSLASCI EEEIEKTLVS 100
IKYGADTIMD LSTGGDLDAI RCAVIEHSSV PIGTVPIYQI LHDVQGDIKN 150
LTIDSMLEVM ERQARQGVSY FTIHCGFLLE HLPKVAKRKM GIVSRGGSLM 200
ASYMMHYHKQ NPFYEAFDEI LAICREYDVT LSLGDSLRPG CLADASDEAQ 250
LAELKVLGEL TLRAWEKDVQ VMIEGPGHVP MNQIERNVKL QKELCHEAPF 300
YVLGPLVTDI AAGYDHIASA IGAAIAAWKG VAMLCYVTPK EHLGLPNAKD 350
VREGILAYKI AAHAADIARG RIGARDRDDA MSDARYAFDW NRQFELALDP 400
DRAKEYHDES LPQEVFKEAK FCSMCGPKFC SYKISQEIVS KHASGESLT 449
Length:449
Mass (Da):49,657
Last modified:December 15, 2003 - v1
Checksum:iDB137D4EFE4DB6A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571662 Genomic DNA. Translation: CAE10947.1.
RefSeqiNP_908047.1. NC_005090.1.
WP_011139730.1. NC_005090.1.

Genome annotation databases

EnsemblBacteriaiCAE10947; CAE10947; WS1941.
GeneIDi2554016.
KEGGiwsu:WS1941.
PATRICi24040822. VBIWolSuc63014_1823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571662 Genomic DNA. Translation: CAE10947.1 .
RefSeqi NP_908047.1. NC_005090.1.
WP_011139730.1. NC_005090.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273121.WS1941.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE10947 ; CAE10947 ; WS1941 .
GeneIDi 2554016.
KEGGi wsu:WS1941.
PATRICi 24040822. VBIWolSuc63014_1823.

Phylogenomic databases

eggNOGi COG0422.
HOGENOMi HOG000224483.
KOi K03147.
OMAi HGKIENL.
OrthoDBi EOG6NWBM5.

Enzyme and pathway databases

UniPathwayi UPA00060 .

Family and domain databases

HAMAPi MF_00089. ThiC.
InterProi IPR002817. ThiC.
[Graphical view ]
Pfami PF01964. ThiC. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00190. thiC. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Entry informationi

Entry nameiTHIC_WOLSU
AccessioniPrimary (citable) accession number: Q7M818
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 15, 2003
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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