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Q7M818

- THIC_WOLSU

UniProt

Q7M818 - THIC_WOLSU

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Protein

Phosphomethylpyrimidine synthase

Gene

thiC

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.UniRule annotation

Catalytic activityi

5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801SubstrateUniRule annotation
Binding sitei109 – 1091SubstrateUniRule annotation
Binding sitei138 – 1381SubstrateUniRule annotation
Binding sitei174 – 1741SubstrateUniRule annotation
Binding sitei274 – 2741SubstrateUniRule annotation
Metal bindingi278 – 2781ZincUniRule annotation
Binding sitei301 – 3011SubstrateUniRule annotation
Metal bindingi342 – 3421ZincUniRule annotation
Metal bindingi422 – 4221Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi425 – 4251Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi430 – 4301Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. lyase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomethylpyrimidine synthaseUniRule annotation (EC:4.1.99.17UniRule annotation)
Alternative name(s):
Hydroxymethylpyrimidine phosphate synthaseUniRule annotation
Short name:
HMP-P synthaseUniRule annotation
Short name:
HMP-phosphate synthaseUniRule annotation
Short name:
HMPP synthaseUniRule annotation
Thiamine biosynthesis protein ThiCUniRule annotation
Gene namesi
Name:thiCUniRule annotation
Ordered Locus Names:WS1941
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Phosphomethylpyrimidine synthasePRO_0000152850Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273121.WS1941.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1963Substrate bindingUniRule annotation
Regioni235 – 2384Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0422.
HOGENOMiHOG000224483.
KOiK03147.
OMAiHGKIENL.
OrthoDBiEOG6NWBM5.

Family and domain databases

HAMAPiMF_00089. ThiC.
InterProiIPR002817. ThiC.
[Graphical view]
PfamiPF01964. ThiC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00190. thiC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7M818-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRQSWIEKRK NDSVRTQLHY ARAGIITEEM QAVANVEGLS EELIRSEVAR
60 70 80 90 100
GRLVIPANIH HLSLSPMGIG VAARTKINAN IGSSSLASCI EEEIEKTLVS
110 120 130 140 150
IKYGADTIMD LSTGGDLDAI RCAVIEHSSV PIGTVPIYQI LHDVQGDIKN
160 170 180 190 200
LTIDSMLEVM ERQARQGVSY FTIHCGFLLE HLPKVAKRKM GIVSRGGSLM
210 220 230 240 250
ASYMMHYHKQ NPFYEAFDEI LAICREYDVT LSLGDSLRPG CLADASDEAQ
260 270 280 290 300
LAELKVLGEL TLRAWEKDVQ VMIEGPGHVP MNQIERNVKL QKELCHEAPF
310 320 330 340 350
YVLGPLVTDI AAGYDHIASA IGAAIAAWKG VAMLCYVTPK EHLGLPNAKD
360 370 380 390 400
VREGILAYKI AAHAADIARG RIGARDRDDA MSDARYAFDW NRQFELALDP
410 420 430 440
DRAKEYHDES LPQEVFKEAK FCSMCGPKFC SYKISQEIVS KHASGESLT
Length:449
Mass (Da):49,657
Last modified:December 15, 2003 - v1
Checksum:iDB137D4EFE4DB6A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571662 Genomic DNA. Translation: CAE10947.1.
RefSeqiNP_908047.1. NC_005090.1.
WP_011139730.1. NC_005090.1.

Genome annotation databases

EnsemblBacteriaiCAE10947; CAE10947; WS1941.
GeneIDi2554016.
KEGGiwsu:WS1941.
PATRICi24040822. VBIWolSuc63014_1823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX571662 Genomic DNA. Translation: CAE10947.1 .
RefSeqi NP_908047.1. NC_005090.1.
WP_011139730.1. NC_005090.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273121.WS1941.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE10947 ; CAE10947 ; WS1941 .
GeneIDi 2554016.
KEGGi wsu:WS1941.
PATRICi 24040822. VBIWolSuc63014_1823.

Phylogenomic databases

eggNOGi COG0422.
HOGENOMi HOG000224483.
KOi K03147.
OMAi HGKIENL.
OrthoDBi EOG6NWBM5.

Enzyme and pathway databases

UniPathwayi UPA00060 .

Family and domain databases

HAMAPi MF_00089. ThiC.
InterProi IPR002817. ThiC.
[Graphical view ]
Pfami PF01964. ThiC. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00190. thiC. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.

Entry informationi

Entry nameiTHIC_WOLSU
AccessioniPrimary (citable) accession number: Q7M818
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 15, 2003
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3