Q7M760 (ZRAN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase Zranb1
EC=3.4.19.12

Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1

Gene names

Name:	Zranb1
Synonyms:	Trabid

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with APC and TRAF6 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Enriched in punctate localization in the cytoplasm By similarity.
Domain	The OTU domain mediates the deubiquitinating activity By similarity. The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin By similarity. The second ankyrin repeat ANK 2 is termed AnkUBD, it interacts with ubiquitin hydrophobic patch and contributes to linkage specificity By similarity. The RanBP2-type zinc fingers, also called NZFs, may provide additional ubiquitin-binding sites when hydrolyzing long 'Lys-63'-linked chains By similarity.
Sequence similarities	Belongs to the peptidase C64 family. Contains 2 ANK repeats. Contains 1 OTU domain. Contains 3 RanBP2-type zinc fingers.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Wnt signaling pathway
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell migration Inferred from sequence or structural similarity. Source: UniProtKB cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein deubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara regulation of cell morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	Ubiquitin thioesterase Zranb1		PRO_0000361554
Regions
	Repeat	260 – 290	31	ANK 1
	Repeat	313 – 340	28	ANK 2
	Domain	432 – 592	161	OTU
	Zinc finger	3 – 33	31	RanBP2-type 1
	Zinc finger	84 – 113	30	RanBP2-type 2
	Zinc finger	149 – 178	30	RanBP2-type 3
Sites
	Active site	443	1	Nucleophile By similarity
	Active site	585	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7M760 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: B7C15B5E01DDF931	FASTA	708	80,934
	10 20 30 40 50 60 MSEHGIKWAC EYCTYENWPS AIKCTMCRAQ RPSGTIITED PFKSGSSDVG RDWDPSSTEG 70 80 90 100 110 120 GSSPLICPDS SARPRVKSSY SMENANKWSC HMCTYLNWPR AIRCTQCLSQ RRTRSPTESP 130 140 150 160 170 180 QSSGSGSRPV AFSVDPCEEY NDRNKLNTRT QHWTCSVCTY ENWAKAKKCV VCDHPRPNNI 190 200 210 220 230 240 EAIELAETEE ASSIINEQDR ARWRGGCSSG NSQRRSPPTT KRDSEVKMDF QRIELAGAVG 250 260 270 280 290 300 TKEELEVDFK KLKQIKNRMK KTDWLFLNAC VGVVEGDLAA IEAYKSSGGD IARQLTADEV 310 320 330 340 350 360 RLLNRPSAFD VGYTLVHLAI RFQRQDMLAI LLTEVSQQAA KCIPAMVCPE LTEQIRREIA 370 380 390 400 410 420 ASLHQRKGDF ACYFLTDLVT FTLPADIEDL PPTVQEKLFD EVLDRDVQKE LEEESPIINW 430 440 450 460 470 480 SLELATRLDS RLYALWNRTA GDCLLDSVLQ ATWGIYDKDS VLRKALHDSL HDCSHWFYTR 490 500 510 520 530 540 WKDWESWYSQ SFGLHFSLRE EQWQEDWAFI LSLASQPGAS LEQTHIFVLA HILRRPIIVY 550 560 570 580 590 600 GVKYYKSFRG ETLGYTRFQG VYLPLLWEQS FCWKSPIALG YTRGHFSALV AMENDGYGNR 610 620 630 640 650 660 GAGANLNTDD DVTITFLPLV DSERKLLHVH FLSAQELGNE EQQEKLLREW LDCCVTEGGV 670 680 690 700 LVAMQKSSRR RNHPLVTQMV EKWLDRYRQI RPCTSLSDGE EDEDDEDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"Human and mouse proteases: a comparative genomic approach." Puente X.S., Sanchez L.M., Overall C.M., Lopez-Otin C. Nat. Rev. Genet. 4:544-558(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC119806 Genomic DNA. No translation available. BN000126 mRNA. Translation: CAD67576.1.
IPI	IPI00136760.
RefSeq	NP_997185.1. NM_207302.1.
UniGene	Mm.389984. Mm.397069. Mm.490863.
3D structure databases
ProteinModelPortal	Q7M760.
SMR	Q7M760. Positions 245-692.
ModBase	Search...
Protein family/group databases
MEROPS	C64.004.
PTM databases
PhosphoSite	Q7M760.
Proteomic databases
PRIDE	Q7M760.
Protocols and materials databases
DNASU	360216.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033265; ENSMUSP00000033265; ENSMUSG00000030967. ENSMUST00000106157; ENSMUSP00000101763; ENSMUSG00000030967.
GeneID	360216.
KEGG	mmu:360216.
UCSC	uc009kcv.2. mouse.
Organism-specific databases
CTD	54764.
MGI	MGI:106441. Zranb1.
Phylogenomic databases
eggNOG	NOG253754.
GeneTree	ENSGT00530000062989.
HOGENOM	HOG000006743.
HOVERGEN	HBG058978.
InParanoid	Q7M760.
KO	K11862.
OMA	EIGNEEQ.
OrthoDB	EOG441Q9X.
Gene expression databases
ArrayExpress	Q7M760.
Bgee	Q7M760.
Genevestigator	Q7M760.
Family and domain databases
InterPro	IPR003323. OTU. IPR001876. Znf_RanBP2. [Graphical view]
Pfam	PF02338. OTU. 1 hit. PF00641. zf-RanBP. 2 hits. [Graphical view]
SMART	SM00547. ZnF_RBZ. 3 hits. [Graphical view]
PROSITE	PS50088. ANK_REPEAT. False negative. PS50802. OTU. 1 hit. PS01358. ZF_RANBP2_1. 3 hits. PS50199. ZF_RANBP2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	400922.
SOURCE	Search...

Entry information

Entry name

ZRAN1_MOUSE

Accession

Primary (citable) accession number: Q7M760

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program