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Protein

N-acetylated-alpha-linked acidic dipeptidase-like protein

Gene

Naaladl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has no NAAG hydrolyzing activity (By similarity). Exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC (By similarity).By similarity

Catalytic activityi

Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi373 – 3731Zinc 1Sequence analysis
Metal bindingi383 – 3831Zinc 1By similarity
Metal bindingi383 – 3831Zinc 2By similarity
Active sitei421 – 4211Nucleophile; for NAALADase activityBy similarity
Metal bindingi422 – 4221Zinc 2By similarity
Metal bindingi450 – 4501Zinc 1By similarity
Metal bindingi550 – 5501Zinc 2By similarity
Active sitei622 – 6221Charge relay systemSequence analysis
Active sitei662 – 6621Charge relay systemSequence analysis
Active sitei685 – 6851Charge relay systemSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM28.011.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylated-alpha-linked acidic dipeptidase-like protein (EC:3.4.17.21)
Short name:
NAALADase L
Gene namesi
Name:Naaladl1
Synonyms:Gm964, Naaladasel
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2685810. Naaladl1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2822Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 745717ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745N-acetylated-alpha-linked acidic dipeptidase-like proteinPRO_0000174124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence analysis
Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence analysis
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence analysis
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence analysis
Glycosylationi593 – 5931N-linked (GlcNAc...)Sequence analysis
Glycosylationi620 – 6201N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ7M758.
PaxDbiQ7M758.
PeptideAtlasiQ7M758.
PRIDEiQ7M758.

PTM databases

iPTMnetiQ7M758.
PhosphoSiteiQ7M758.

Expressioni

Gene expression databases

BgeeiQ7M758.
GenevisibleiQ7M758. MM.

Interactioni

Protein-protein interaction databases

IntActiQ7M758. 1 interaction.
STRINGi10090.ENSMUSP00000044231.

Structurei

3D structure databases

ProteinModelPortaliQ7M758.
SMRiQ7M758. Positions 43-745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 584317NAALADaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi635 – 6395Poly-Ala

Sequence similaritiesi

Belongs to the peptidase M28 family. M28B subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
GeneTreeiENSGT00550000074421.
HOGENOMiHOG000211921.
HOVERGENiHBG051639.
InParanoidiQ7M758.
KOiK01301.
OMAiTSMDLAY.
OrthoDBiEOG7QK0BC.
PhylomeDBiQ7M758.
TreeFamiTF312981.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR003137. PA_domain.
IPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7M758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWVKILGVA LGAAALLGLG IILGHFAIPK ATSPLTSSTS DSQDLDLAIL
60 70 80 90 100
DSVMGQLDAS RIRENLRELS KEPHVATSPR DEALVQLLLG RWKDTATGLD
110 120 130 140 150
SAKTYEYRVL LSFPNAEQPN SVEVVGPNGT TFHSFQPFEK NLTGEQAGPN
160 170 180 190 200
VLQPYAAYAP PGTPKGLLVY ANQGSEEDFK ELETQGINLE GTIALTRYGG
210 220 230 240 250
VGRGAKAINA AKHGVVGVLV YTDPGDINDG KSLPNETFPN SWRLPPSGVE
260 270 280 290 300
RGSYYEYFGD PLTPYLPAHP SSFRLDPHNT SGFPPIPTQP IGFEDARDLL
310 320 330 340 350
CNLTGTSAPA FWQGALGCEY KLGPGFEPNG SFPAGSEVKV SVHNRLELRT
360 370 380 390 400
SSNVLGIIQG AVEPDRYVIY GNHRDSWVHG AVDPSSGTAV LLEISRVLGT
410 420 430 440 450
LLKKGTWRPR RSIIFASWGA EEFGLIGSTE FTEEFLSKLQ ERTVAYINVD
460 470 480 490 500
ISVFSNATLR AQGTPPVQSV IFSATKEISA PGSSGLSIYD NWIRYTNRTS
510 520 530 540 550
PVYGLVPSLG TLGAGSDYAA FVHFLGITSM DLAYTYDRSK TSARIYPTYH
560 570 580 590 600
TAFDTFDYVE KFLDPGFSSH QAVARTAGSV LLRLSDSLFL PLNVSDYSET
610 620 630 640 650
LQSFLQAAQE ALGTQLEKQN ISLGPLVTAV ANFKAAAASL GEHILTLQKS
660 670 680 690 700
SPDPLQVRMV NDQLMLLERA FLNPRAFPEE RHYSHVLWAP NTASVDTFPG
710 720 730 740
LANAYAKAQE INSGSEAWAE VQRQLSIVVT ALEGAAATLV PVADL
Length:745
Mass (Da):80,513
Last modified:December 15, 2003 - v1
Checksum:i5DC21DFEAD6D34CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131114 Genomic DNA. No translation available.
BN000129 mRNA. Translation: CAD67582.1.
CCDSiCCDS29494.1.
RefSeqiNP_001009546.1. NM_001009546.1.
UniGeneiMm.359041.

Genome annotation databases

EnsembliENSMUST00000044451; ENSMUSP00000044231; ENSMUSG00000054999.
GeneIDi381204.
KEGGimmu:381204.
UCSCiuc008ghk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131114 Genomic DNA. No translation available.
BN000129 mRNA. Translation: CAD67582.1.
CCDSiCCDS29494.1.
RefSeqiNP_001009546.1. NM_001009546.1.
UniGeneiMm.359041.

3D structure databases

ProteinModelPortaliQ7M758.
SMRiQ7M758. Positions 43-745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7M758. 1 interaction.
STRINGi10090.ENSMUSP00000044231.

Protein family/group databases

MEROPSiM28.011.

PTM databases

iPTMnetiQ7M758.
PhosphoSiteiQ7M758.

Proteomic databases

MaxQBiQ7M758.
PaxDbiQ7M758.
PeptideAtlasiQ7M758.
PRIDEiQ7M758.

Protocols and materials databases

DNASUi381204.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044451; ENSMUSP00000044231; ENSMUSG00000054999.
GeneIDi381204.
KEGGimmu:381204.
UCSCiuc008ghk.1. mouse.

Organism-specific databases

CTDi10004.
MGIiMGI:2685810. Naaladl1.

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
GeneTreeiENSGT00550000074421.
HOGENOMiHOG000211921.
HOVERGENiHBG051639.
InParanoidiQ7M758.
KOiK01301.
OMAiTSMDLAY.
OrthoDBiEOG7QK0BC.
PhylomeDBiQ7M758.
TreeFamiTF312981.

Miscellaneous databases

PROiQ7M758.
SOURCEiSearch...

Gene expression databases

BgeeiQ7M758.
GenevisibleiQ7M758. MM.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR003137. PA_domain.
IPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Pearman C., Haakenson W.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6J.
  2. "Human and mouse proteases: a comparative genomic approach."
    Puente X.S., Sanchez L.M., Overall C.M., Lopez-Otin C.
    Nat. Rev. Genet. 4:544-558(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiNALDL_MOUSE
AccessioniPrimary (citable) accession number: Q7M758
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 15, 2003
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.