Q7M729 (SCN4B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium channel subunit beta-4

Gene names

Name:	Scn4b
Synonyms:	Gm1471

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation By similarity.
Subunit structure	The voltage-sensitive sodium channel consists of an ion conducting pore forming alpha-subunit regulated by one or more beta-1, beta-2, beta-3 and/or beta-4 subunits. Beta-1 and beta-3 are non-covalently associated with alpha, while beta-2 and beta-4 are covalently linked by disulfide bonds. Associates with SCN2A By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Probable.
Post-translational modification	Contains a number of interchain disulfide bonds with SCN2A By similarity.
Sequence similarities	Belongs to the sodium channel auxiliary subunit SCN4B (TC 8.A.17) family. [View classification] Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Ligand	Sodium
Molecular function	Ion channel Sodium channel Voltage-gated channel
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	AV node cell to bundle of His cell communication Inferred from electronic annotation. Source: Compara cardiac muscle contraction Inferred from electronic annotation. Source: Compara positive regulation of sodium ion transport Inferred from electronic annotation. Source: Compara regulation of cardiac muscle cell action potential involved in contraction Inferred from electronic annotation. Source: Compara regulation of heart rate by cardiac conduction Inferred from electronic annotation. Source: Compara regulation of sodium ion transmembrane transporter activity Inferred from electronic annotation. Source: Compara regulation of ventricular cardiac muscle cell membrane repolarization Inferred from electronic annotation. Source: Compara
Cellular_component	intercalated disc Inferred from direct assay PubMed 17884088. Source: BHF-UCL voltage-gated sodium channel complex Inferred by curator PubMed 20566860. Source: MGI
Molecular_function	sodium channel regulator activity Inferred from electronic annotation. Source: Compara voltage-gated sodium channel activity Inferred from mutant phenotype PubMed 20566860. Source: MGI voltage-gated sodium channel activity involved in regulation of cardiac muscle cell action potential Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 228	198	Sodium channel subunit beta-4		PRO_0000014938
Regions
	Topological domain	31 – 161	131	Extracellular Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Topological domain	183 – 228	46	Cytoplasmic Potential
	Domain	31 – 148	118	Ig-like C2-type
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Disulfide bond	53 ↔ 131		By similarity
	Disulfide bond	58		Interchain; with alpha subunit By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7M729 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: BD4839047672C911	FASTA	228	25,192
	10 20 30 40 50 60 MSRAGNRGNT QARWLGTGLL GLFLLPMYLS LEVSVGKATT IYAINGSSIL LPCTFSSCYG 70 80 90 100 110 120 FENLYFKWSY NNSETSRILI DGIVKNDKSD PKVRVKDDDR ITLEGSTKEK TNNISILLSD 130 140 150 160 170 180 LEFSDTGRYT CFVRNPKEKD LNNSATIFLQ VVDKLEKVDN TVTLIILAVV GGVIGLLVCI 190 200 210 220 LLLKKLITFI LKKTREKKKE CLVSSSGNDN TENGLPGSKA EEKPPTKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of the mouse sodium channel beta 4 subunit (scn4b)." Puhl H.L. III, Ikeda S.R. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster. Tissue: Spinal ganglion.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Sodium channel beta4, a new disulfide-linked auxiliary subunit with similarity to beta2." Yu F.H., Westenbroek R.E., Silos-Santiago I., McCormick K.A., Lawson D., Ge P., Ferriera H., Lilly J., DiStefano P.S., Catterall W.A., Scheuer T., Curtis R. J. Neurosci. 23:7577-7585(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY669079 mRNA. Translation: AAU05394.1. AC122305 Genomic DNA. No translation available. AC122504 Genomic DNA. No translation available. BK001031 mRNA. Translation: DAA01205.1.
IPI	IPI00395089.
RefSeq	NP_001013408.1. NM_001013390.2.
UniGene	Mm.335112.
3D structure databases
ProteinModelPortal	Q7M729.
SMR	Q7M729. Positions 37-154.
ModBase	Search...
PTM databases
PhosphoSite	Q7M729.
Proteomic databases
PRIDE	Q7M729.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000060125; ENSMUSP00000062507; ENSMUSG00000046480.
GeneID	399548.
KEGG	mmu:399548.
Organism-specific databases
CTD	6330.
MGI	MGI:2687406. Scn4b.
Phylogenomic databases
eggNOG	NOG46712.
GeneTree	ENSGT00640000091161.
HOGENOM	HOG000048715.
HOVERGEN	HBG056581.
InParanoid	Q7M729.
KO	K04848.
OMA	REKKKEC.
OrthoDB	EOG43JC5V.
Gene expression databases
ArrayExpress	Q7M729.
Bgee	Q7M729.
CleanEx	MM_SCN4B.
Genevestigator	Q7M729.
GermOnline	ENSMUSG00000046480. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR013106. Ig_V-set. [Graphical view]
Pfam	PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	406014.
SOURCE	Search...

Entry information

Entry name

SCN4B_MOUSE

Accession

Primary (citable) accession number: Q7M729

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program