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Protein

Kinesin-like protein KIF27

Gene

Kif27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in motile ciliogenesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 918ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATPase activity Source: GO_Central
  2. ATP binding Source: UniProtKB-KW
  3. microtubule motor activity Source: GO_Central

GO - Biological processi

  1. cilium assembly Source: UniProtKB
  2. epithelial cilium movement Source: MGI
  3. metabolic process Source: GOC
  4. smoothened signaling pathway Source: GO_Central
  5. ventricular system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF27
Gene namesi
Name:Kif27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1922300. Kif27.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Cell projectioncilium 1 Publication
Note: Localizes to centrioles and basal bodies.

GO - Cellular componenti

  1. cilium Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: MGI
  4. kinesin complex Source: GO_Central
  5. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13941394Kinesin-like protein KIF27PRO_0000307144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1084 – 1084Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ7M6Z4.
PaxDbiQ7M6Z4.
PRIDEiQ7M6Z4.

PTM databases

PhosphoSiteiQ7M6Z4.

Expressioni

Gene expression databases

BgeeiQ7M6Z4.
CleanExiMM_KIF27.
GenevestigatoriQ7M6Z4.

Interactioni

Subunit structurei

Interacts with STK36.1 Publication

Protein-protein interaction databases

DIPiDIP-59753N.
STRINGi10090.ENSMUSP00000043304.

Structurei

3D structure databases

ProteinModelPortaliQ7M6Z4.
SMRiQ7M6Z4. Positions 2-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 341337Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili352 – 41867Sequence AnalysisAdd
BLAST
Coiled coili498 – 55457Sequence AnalysisAdd
BLAST
Coiled coili709 – 891183Sequence AnalysisAdd
BLAST
Coiled coili921 – 1078158Sequence AnalysisAdd
BLAST
Coiled coili1118 – 115235Sequence AnalysisAdd
BLAST
Coiled coili1186 – 122641Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1282 – 12854Poly-Ser

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIF27 subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00770000120453.
HOGENOMiHOG000068072.
HOVERGENiHBG054858.
InParanoidiQ7M6Z4.
KOiK10395.
OMAiAKLRVQX.
OrthoDBiEOG7K3TK3.
PhylomeDBiQ7M6Z4.
TreeFamiTF325946.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7M6Z4-1) [UniParc]FASTAAdd to basket

Also known as: KIF27A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEIPIKVAV RIRPLLCKEV LHNHQVCVRD IPNTQQIIIG RDRVFTFDFV
60 70 80 90 100
FGKNSTQDEV YNTCIKPLVL SLIEGYNATV FAYGQTGSGK TYTIGGGHVA
110 120 130 140 150
SVVEGQKGII PRAIQEIFQS ISENPSIDFK IKVSYIEVYK EDLRDLLELE
160 170 180 190 200
TSMKDLHIRE DEKGNTVIVG AKECQVESVE DVMSLLQVGN AARHTGTTQM
210 220 230 240 250
NEHSSRSHAI FTISVCQVEK NAEAAENGEW YSHRHIVSKF HFVDLAGSER
260 270 280 290 300
VTKTGNTGER FKESIQINSG LLALGNVISA LGDPRRKSSH IPYRDAKITR
310 320 330 340 350
LLKDSLGGSA KTVMITCVSP SSSDFDESLN SLKYANRARN IRNKPALNIS
360 370 380 390 400
PQADRMDEME FEIKLLREAL QSHQASISQA SQASSENVPD QNRIHSLEEQ
410 420 430 440 450
VAQLQEECLG YQDCIEQAFA FLVDLKDAVK LNQKQQHKLQ EWFSRTQEVR
460 470 480 490 500
KAVLTPLPGN QGIGNLEEGP QHLTVLQLKR ELKKYQCALA ADQVVFTQKD
510 520 530 540 550
LELEELRTQV QLMMQESKGH AVSLKEAQKV NRLQNEKIIE QQLLVDQLSA
560 570 580 590 600
ELAKRSLSVP TSAKESCGDG PDARASEKRP HTAPFESHWG HYVYIPSRQD
610 620 630 640 650
FKKVCSSTPV YSLDQVFAGF RTRSQMLMGH LEDQDEVLHC QFSDNSDDED
660 670 680 690 700
SEGQEKPRVR SRSHSWAKKP GSVCSLVELS DTQAESQRSY LGNGDLKMES
710 720 730 740 750
LQESQEINLQ KLRTSELILN KAKQKMRELT INIRMKEDLI KELIKTGNNA
760 770 780 790 800
KSVSRQYSLK VTKLEHEAEQ AKVELTETRK QLQELESKDL SDVALKVKLQ
810 820 830 840 850
KEFRKKMDAA KMRVQVLQKK QQDSKKLASL SIQNEKRASE LEHNVDHLKY
860 870 880 890 900
QKVQLQRRLR EEGEKKKQLD AEIKRDQQKI KELQLKAGQG EGLNPKAEDQ
910 920 930 940 950
DGFNLNRRKS PFRSGDQFQK LDEQRKWLDE EVEKVLSQRQ ELEMLEEDLK
960 970 980 990 1000
KREAIVSKKE ALLQEKSLLE NKKLRSSQAL STDGLKISAR LNLLDQELSE
1010 1020 1030 1040 1050
KSLQLESSPT EEKMKISEQV QALQRERDQL QRQRNSVDER LKHGRVLSPK
1060 1070 1080 1090 1100
EEHLLFQLEE GIEALEAAIE FKNESIQSRQ NSLKASFQNL SQSEANVLEK
1110 1120 1130 1140 1150
LVCLNITEIR AILFKYFNKV INLRETERKQ QLQNKEMKMK VLERDNVVHE
1160 1170 1180 1190 1200
LESALEHLRL QCDRRLTLQQ KEHEQKMQLL LQHFKDQDGD SIIETLKNYE
1210 1220 1230 1240 1250
DKIQQLEKDL YFYKKTSRDL KKRLKDPAQG AAQWQRTLTE HHDAGDGVLN
1260 1270 1280 1290 1300
PEETTVLSEE LKWASRTENT KLNGSEREVD NSSSSLKTQP LTQQIPEDGP
1310 1320 1330 1340 1350
DSLPARSSIA PSSGQLQSIA DKTEARPFTH SQSPVPHQFQ PVRSIGPLQG
1360 1370 1380 1390
VKPVKLCRRE LRQISAMELS LRRCSLGAGG RSMTADSLED PEEN
Length:1,394
Mass (Da):158,956
Last modified:December 15, 2003 - v1
Checksum:iAC5F7CCD2CA61D6B
GO
Isoform 2 (identifier: Q7M6Z4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-484: Missing.
     485-486: YQ → ML

Show »
Length:910
Mass (Da):104,716
Checksum:i375B4C11BBFAB80D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1377 – 13771G → E in BAC28083 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 484484Missing in isoform 2. 1 PublicationVSP_028607Add
BLAST
Alternative sequencei485 – 4862YQ → ML in isoform 2. 1 PublicationVSP_028608

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC116646 mRNA. Translation: AAI16647.1.
AK032912 mRNA. Translation: BAC28083.1.
BK001056 mRNA. Translation: DAA01314.1.
CCDSiCCDS26570.1. [Q7M6Z4-1]
RefSeqiNP_780423.2. NM_175214.3. [Q7M6Z4-1]
XP_006517489.1. XM_006517426.2. [Q7M6Z4-1]
XP_006517490.1. XM_006517427.2. [Q7M6Z4-1]
XP_006517491.1. XM_006517428.2. [Q7M6Z4-1]
XP_006517492.1. XM_006517429.2. [Q7M6Z4-1]
XP_006517493.1. XM_006517430.2. [Q7M6Z4-1]
XP_006517494.1. XM_006517431.2. [Q7M6Z4-1]
XP_006517495.1. XM_006517432.2. [Q7M6Z4-1]
XP_006517496.1. XM_006517433.2. [Q7M6Z4-1]
XP_006517497.1. XM_006517434.2. [Q7M6Z4-1]
XP_006517498.1. XM_006517435.2. [Q7M6Z4-1]
XP_006517499.1. XM_006517436.2. [Q7M6Z4-1]
UniGeneiMm.446094.

Genome annotation databases

EnsembliENSMUST00000043605; ENSMUSP00000043304; ENSMUSG00000060176. [Q7M6Z4-1]
GeneIDi75050.
KEGGimmu:75050.
UCSCiuc007qtq.1. mouse. [Q7M6Z4-1]
uc011zao.1. mouse. [Q7M6Z4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC116646 mRNA. Translation: AAI16647.1.
AK032912 mRNA. Translation: BAC28083.1.
BK001056 mRNA. Translation: DAA01314.1.
CCDSiCCDS26570.1. [Q7M6Z4-1]
RefSeqiNP_780423.2. NM_175214.3. [Q7M6Z4-1]
XP_006517489.1. XM_006517426.2. [Q7M6Z4-1]
XP_006517490.1. XM_006517427.2. [Q7M6Z4-1]
XP_006517491.1. XM_006517428.2. [Q7M6Z4-1]
XP_006517492.1. XM_006517429.2. [Q7M6Z4-1]
XP_006517493.1. XM_006517430.2. [Q7M6Z4-1]
XP_006517494.1. XM_006517431.2. [Q7M6Z4-1]
XP_006517495.1. XM_006517432.2. [Q7M6Z4-1]
XP_006517496.1. XM_006517433.2. [Q7M6Z4-1]
XP_006517497.1. XM_006517434.2. [Q7M6Z4-1]
XP_006517498.1. XM_006517435.2. [Q7M6Z4-1]
XP_006517499.1. XM_006517436.2. [Q7M6Z4-1]
UniGeneiMm.446094.

3D structure databases

ProteinModelPortaliQ7M6Z4.
SMRiQ7M6Z4. Positions 2-374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59753N.
STRINGi10090.ENSMUSP00000043304.

PTM databases

PhosphoSiteiQ7M6Z4.

Proteomic databases

MaxQBiQ7M6Z4.
PaxDbiQ7M6Z4.
PRIDEiQ7M6Z4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043605; ENSMUSP00000043304; ENSMUSG00000060176. [Q7M6Z4-1]
GeneIDi75050.
KEGGimmu:75050.
UCSCiuc007qtq.1. mouse. [Q7M6Z4-1]
uc011zao.1. mouse. [Q7M6Z4-2]

Organism-specific databases

CTDi55582.
MGIiMGI:1922300. Kif27.

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00770000120453.
HOGENOMiHOG000068072.
HOVERGENiHBG054858.
InParanoidiQ7M6Z4.
KOiK10395.
OMAiAKLRVQX.
OrthoDBiEOG7K3TK3.
PhylomeDBiQ7M6Z4.
TreeFamiTF325946.

Miscellaneous databases

NextBioi342092.
PROiQ7M6Z4.
SOURCEiSearch...

Gene expression databases

BgeeiQ7M6Z4.
CleanExiMM_KIF27.
GenevestigatoriQ7M6Z4.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 726-1394 (ISOFORM 1).
    Strain: C57BL/6J.
  3. "Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs."
    Oduru S., Campbell J.L., Karri S., Hendry W.J., Khan S.A., Williams S.C.
    BMC Genomics 4:22-22(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION (ISOFORM 1).
  4. "A proteomics approach to identify the ubiquitinated proteins in mouse heart."
    Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J.
    Biochem. Biophys. Res. Commun. 357:731-736(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1084.
    Tissue: Heart.
  5. "Fused has evolved divergent roles in vertebrate Hedgehog signalling and motile ciliogenesis."
    Wilson C.W., Nguyen C.T., Chen M.H., Yang J.H., Gacayan R., Huang J., Chen J.N., Chuang P.T.
    Nature 459:98-102(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STK36.

Entry informationi

Entry nameiKIF27_MOUSE
AccessioniPrimary (citable) accession number: Q7M6Z4
Secondary accession number(s): Q14AX5, Q8BMB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: December 15, 2003
Last modified: April 1, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.