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Protein

N-lysine methyltransferase SMYD2

Gene

Smyd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860' (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei240Peptide substrate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 90MYND-typePROSITE-ProRule annotationAdd BLAST39

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-3214841 PKMTs methylate histone lysines
R-RNO-6804760 Regulation of TP53 Activity through Methylation

Names & Taxonomyi

Protein namesi
Recommended name:
N-lysine methyltransferase SMYD2 (EC:2.1.1.-)
Alternative name(s):
Histone methyltransferase SMYD2 (EC:2.1.1.43)
SET and MYND domain-containing protein 2
Gene namesi
Name:Smyd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi727785 Smyd2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002183111 – 433N-lysine methyltransferase SMYD2Add BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei283PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7M6Z3
PRIDEiQ7M6Z3

PTM databases

iPTMnetiQ7M6Z3
PhosphoSitePlusiQ7M6Z3

Expressioni

Gene expression databases

BgeeiENSRNOG00000003583
GenevisibleiQ7M6Z3 RN

Interactioni

Subunit structurei

Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1. Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004783

Structurei

3D structure databases

ProteinModelPortaliQ7M6Z3
SMRiQ7M6Z3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 241SETPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 19S-adenosyl-L-methionine bindingBy similarity3
Regioni183 – 185Peptide substrate bindingBy similarity3
Regioni206 – 207S-adenosyl-L-methionine bindingBy similarity2
Regioni258 – 260S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 90MYND-typePROSITE-ProRule annotationAdd BLAST39

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2084 Eukaryota
COG2940 LUCA
GeneTreeiENSGT00530000063077
HOGENOMiHOG000007850
HOVERGENiHBG098536
InParanoidiQ7M6Z3
KOiK11426
OMAiLAKQKTH
OrthoDBiEOG091G066T
PhylomeDBiQ7M6Z3
TreeFamiTF106487

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR001214 SET_dom
IPR011990 TPR-like_helical_dom_sf
IPR002893 Znf_MYND
PfamiView protein in Pfam
PF00856 SET, 1 hit
PF01753 zf-MYND, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50280 SET, 1 hit
PS01360 ZF_MYND_1, 1 hit
PS50865 ZF_MYND_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q7M6Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAEARGGLE RFCSAGKGRG LRALRPFHVG DLLFSCPAYA CVLTVGERGH
60 70 80 90 100
HCECCFARKE GLSKCGRCKQ AFYCDVECQK EDWPLHKLEC SSMVVFGENW
110 120 130 140 150
NPSETVRLTA RILAKQKMHP ERTPSEKLLA VREFESHLDK LDNEKKDLIQ
160 170 180 190 200
SDIAALHQFY SKHLEFPDHS SLVVLFAQVN CNGFTIEDEE LSHLGSAIFP
210 220 230 240 250
DVALMNHSCC PNVIVTYKGT LAEVRAVQEI HPGDEVFTSY IDLLYPTEDR
260 270 280 290 300
NDRLRDSYFF TCECRECTTK DKDKAKVEIR KLSNPPQAEA IRDMVRYARN
310 320 330 340 350
VIEEFRRAKH YKSPSELLEI CELSQEKMSS VFEDSNVYML HMMYQAMGVC
360 370 380 390 400
LYMQDWEGAL KYGQKIIKPY SKHYPVYSLN VASMWLKLGR LYMGLENKAA
410 420 430
GEKALKKAIA IMEIAHGKDH PYISEIKQEI ESH
Length:433
Mass (Da):49,648
Last modified:December 15, 2003 - v1
Checksum:i7CE05492B8CA1578
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK001057 mRNA Translation: DAA01315.1
RefSeqiNP_996733.1, NM_206851.1
UniGeneiRn.7052

Genome annotation databases

EnsembliENSRNOT00000004783; ENSRNOP00000004783; ENSRNOG00000003583
GeneIDi289372
KEGGirno:289372

Similar proteinsi

Entry informationi

Entry nameiSMYD2_RAT
AccessioniPrimary (citable) accession number: Q7M6Z3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 15, 2003
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health