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Q7M6Y3 (PICAL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol-binding clathrin assembly protein
Alternative name(s):
Clathrin assembly lymphoid myeloid leukemia
Short name=CALM
Gene names
Name:Picalm
Synonyms:Calm, Fit1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length660 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Plays a crucial role in fetal and adult hematopoiesis, and normal prenatal and postnatal growth and viability. Ref.1 Ref.4

Subunit structure

Binds clathrin and phosphatidylinositol 4,5-bisphosphate By similarity.Interacts with FAM64A; this interaction may change the subcellular location into the nucleus By similarity.

Subcellular location

Membraneclathrin-coated pit By similarity. Golgi apparatus By similarity. Cytoplasmic vesicleclathrin-coated vesicle By similarity. Nucleus By similarity. Note: Colocalized with clathrin in the Golgi area By similarity. Interaction with FAM64A may target PICALM to the nucleus in some cells By similarity.

Tissue specificity

Skins and livers of 1-week-old mice. Ref.1

Sequence similarities

Belongs to the PICALM/SNAP91 family.

Contains 1 ENTH (epsin N-terminal homology) domain.

Sequence caution

The sequence BAC39454.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCoated pit
Cytoplasmic vesicle
Golgi apparatus
Membrane
Nucleus
   Coding sequence diversityAlternative splicing
   Molecular functionDevelopmental protein
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxonogenesis

Inferred from mutant phenotype PubMed 18842885. Source: BHF-UCL

clathrin coat assembly

Inferred from electronic annotation. Source: InterPro

dendrite morphogenesis

Inferred from mutant phenotype PubMed 18842885. Source: BHF-UCL

endosomal transport

Inferred from electronic annotation. Source: Ensembl

hemopoiesis

Inferred from mutant phenotype Ref.1. Source: UniProtKB

negative regulation of receptor-mediated endocytosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

receptor internalization

Inferred from electronic annotation. Source: Ensembl

receptor-mediated endocytosis

Inferred from direct assay Ref.1. Source: UniProtKB

regulation of protein localization

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentAP-2 adaptor complex

Inferred from electronic annotation. Source: Ensembl

Golgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

coated pit

Inferred from direct assay Ref.1. Source: UniProtKB

intracellular

Inferred from direct assay PubMed 18842885. Source: BHF-UCL

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function1-phosphatidylinositol binding

Inferred from sequence or structural similarity. Source: UniProtKB

clathrin binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7M6Y3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7M6Y3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.
     506-510: Missing.
     594-601: Missing.
Isoform 3 (identifier: Q7M6Y3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.
     506-510: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q7M6Y3-4)

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q7M6Y3-5)

The sequence of this isoform differs from the canonical sequence as follows:
     506-510: Missing.
Isoform 6 (identifier: Q7M6Y3-6)

The sequence of this isoform differs from the canonical sequence as follows:
     594-601: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 660659Phosphatidylinositol-binding clathrin assembly protein
PRO_0000187063

Regions

Domain14 – 145132ENTH
Region221 – 29474Interaction with FAM64A By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity

Natural variations

Alternative sequence420 – 46950Missing in isoform 2, isoform 3 and isoform 4.
VSP_050684
Alternative sequence506 – 5105Missing in isoform 2, isoform 3 and isoform 5.
VSP_050685
Alternative sequence594 – 6018Missing in isoform 2 and isoform 6.
VSP_050686

Experimental info

Sequence conflict5221N → S in AAH21491. Ref.2
Sequence conflict5221N → S in AAH23843. Ref.2
Sequence conflict5221N → S in AAH25566. Ref.2
Sequence conflict5221N → S in AAH57683. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 7FB206508281BCDF

FASTA66071,543
        10         20         30         40         50         60 
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN EMNVNIPQLA 

        70         80         90        100        110        120 
DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR NTLFNLSNFL DKSGLQGYDM 

       130        140        150        160        170        180 
STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR GADGVMRTMN TEKLLKTVPI IQNQMDALLD 

       190        200        210        220        230        240 
FNVNSNELTN GVINAAFMLL FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF 

       250        260        270        280        290        300 
LTRMTRISEF LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT 

       310        320        330        340        350        360 
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP HTSLTTAASP 

       370        380        390        400        410        420 
VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT FHPSVHAMSA APQGASTWGD 

       430        440        450        460        470        480 
PFSATLDAVE DAIPSLNPFL TKSSGDVHLP IASDVSTFTT RTPTHEMFVG FSPSPVAQPH 

       490        500        510        520        530        540 
SSAGLNVDFE SVFGNKSTNV AVDSGGFDEL GGLLKPTVAS QNQSLPVAKL PPNKLVSDDL 

       550        560        570        580        590        600 
DSSLANLVGN LGIGNGTTKN DVSWSQPGEK KLTGGSNWQP KVAPTTAWSA ATMNGMHFPQ 

       610        620        630        640        650        660 
YAPPVMAYPA TTPTGMIGYG IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG PVSGAQIQFM 

« Hide

Isoform 2 [UniParc].

Checksum: 825D7F7B7154DD8A
Show »

FASTA59764,648
Isoform 3 [UniParc].

Checksum: C365144C975C2B50
Show »

FASTA60565,623
Isoform 4 [UniParc].

Checksum: 86E8189B6C05F005
Show »

FASTA61066,184
Isoform 5 [UniParc].

Checksum: F729CEF5D3D82781
Show »

FASTA65570,982
Isoform 6 [UniParc].

Checksum: 0CEEEEA9225CC446
Show »

FASTA65270,568

References

« Hide 'large scale' references
[1]"Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities in fit1 mice."
Klebig M.L., Wall M.D., Potter M.D., Rowe E.L., Carpenter D.A., Rinchik E.M.
Proc. Natl. Acad. Sci. U.S.A. 100:8360-8365(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Strain: BALB/cRl.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3; 4; 5 AND 6).
Strain: Czech II and FVB/N.
Tissue: Mammary tumor and Retina.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 143-660 (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Epididymis and Kidney.
[4]"Mutations in the murine fitness 1 gene result in defective hematopoiesis."
Potter M.D., Shinpock S.G., Popp R.A., Godfrey V., Carpenter D.A., Bernstein A., Johnson D.K., Rinchik E.M.
Blood 90:1850-1857(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY206701 mRNA. Translation: AAO17153.1.
BK001028 mRNA. Translation: DAA01470.1.
BC011470 mRNA. Translation: AAH11470.1.
BC021491 mRNA. Translation: AAH21491.1.
BC023843 mRNA. Translation: AAH23843.1.
BC025566 mRNA. Translation: AAH25566.1.
BC027116 mRNA. Translation: AAH27116.1.
BC057683 mRNA. Translation: AAH57683.1.
AK085472 mRNA. Translation: BAC39454.2. Different initiation.
AK162360 mRNA. Translation: BAE36872.1.
RefSeqNP_001239449.1. NM_001252520.1.
NP_001239450.1. NM_001252521.1.
NP_001239451.1. NM_001252522.1.
NP_001239452.1. NM_001252523.1.
NP_001239453.1. NM_001252524.1.
NP_666306.2. NM_146194.4.
UniGeneMm.235175.
Mm.393563.

3D structure databases

ProteinModelPortalQ7M6Y3.
SMRQ7M6Y3. Positions 20-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231417. 1 interaction.
IntActQ7M6Y3. 3 interactions.
MINTMINT-1850199.
STRING10090.ENSMUSP00000051092.

PTM databases

PhosphoSiteQ7M6Y3.

Proteomic databases

PaxDbQ7M6Y3.
PRIDEQ7M6Y3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049537; ENSMUSP00000051092; ENSMUSG00000039361. [Q7M6Y3-1]
GeneID233489.
KEGGmmu:233489.
UCSCuc009igq.2. mouse. [Q7M6Y3-1]
uc009igr.2. mouse. [Q7M6Y3-5]
uc009igs.2. mouse. [Q7M6Y3-6]
uc009igt.2. mouse. [Q7M6Y3-4]
uc009igu.2. mouse. [Q7M6Y3-3]

Organism-specific databases

CTD8301.
MGIMGI:2385902. Picalm.

Phylogenomic databases

eggNOGNOG267212.
GeneTreeENSGT00390000008805.
HOVERGENHBG049391.
InParanoidQ7M6Y3.
OMAWNAATMA.
OrthoDBEOG76QFM8.
PhylomeDBQ7M6Y3.
TreeFamTF314861.

Gene expression databases

BgeeQ7M6Y3.
GenevestigatorQ7M6Y3.

Family and domain databases

Gene3D1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProIPR011417. ANTH_dom.
IPR014712. Clathrin_Pinositid-bd_GAT-like.
IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
[Graphical view]
PfamPF07651. ANTH. 1 hit.
[Graphical view]
SMARTSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMSSF48464. SSF48464. 1 hit.
PROSITEPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPICALM. mouse.
NextBio381725.
PROQ7M6Y3.
SOURCESearch...

Entry information

Entry namePICAL_MOUSE
AccessionPrimary (citable) accession number: Q7M6Y3
Secondary accession number(s): Q3TS04 expand/collapse secondary AC list , Q811P1, Q8BUF6, Q8CIH8, Q8R0A9, Q8R3E1, Q8VDN5, Q921L0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: December 15, 2003
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot