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Q7M6Y3

- PICAL_MOUSE

UniProt

Q7M6Y3 - PICAL_MOUSE

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Protein

Phosphatidylinositol-binding clathrin assembly protein

Gene

Picalm

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Plays a crucial role in fetal and adult hematopoiesis, and normal prenatal and postnatal growth and viability.2 Publications

GO - Molecular functioni

  1. 1-phosphatidylinositol binding Source: UniProtKB
  2. clathrin adaptor activity Source: Alzheimers_University_of_Toronto
  3. clathrin binding Source: UniProtKB

GO - Biological processi

  1. axonogenesis Source: BHF-UCL
  2. cargo loading into vesicle Source: Alzheimers_University_of_Toronto
  3. cell proliferation Source: Alzheimers_University_of_Toronto
  4. clathrin coat assembly Source: InterPro
  5. clathrin-mediated endocytosis Source: Alzheimers_University_of_Toronto
  6. dendrite morphogenesis Source: BHF-UCL
  7. endosomal transport Source: Ensembl
  8. hemopoiesis Source: UniProtKB
  9. iron ion homeostasis Source: Alzheimers_University_of_Toronto
  10. iron ion import into cell Source: Alzheimers_University_of_Toronto
  11. negative regulation of gene expression Source: Alzheimers_University_of_Toronto
  12. negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process Source: Alzheimers_University_of_Toronto
  13. negative regulation of receptor-mediated endocytosis Source: Ensembl
  14. positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process Source: Alzheimers_University_of_Toronto
  15. positive regulation of beta-amyloid formation Source: Alzheimers_University_of_Toronto
  16. positive regulation of neuron death Source: Alzheimers_University_of_Toronto
  17. positive regulation of transcription, DNA-templated Source: Ensembl
  18. receptor internalization Source: Ensembl
  19. receptor-mediated endocytosis Source: UniProtKB
  20. regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process Source: Alzheimers_University_of_Toronto
  21. regulation of protein localization Source: Ensembl
  22. synaptic vesicle maturation Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-binding clathrin assembly protein
Alternative name(s):
Clathrin assembly lymphoid myeloid leukemia
Short name:
CALM
Gene namesi
Name:Picalm
Synonyms:Calm, Fit1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2385902. Picalm.

Subcellular locationi

Membraneclathrin-coated pit By similarity. Golgi apparatus By similarity. Cytoplasmic vesicleclathrin-coated vesicle By similarity. Nucleus By similarity
Note: Colocalized with clathrin in the Golgi area. Interaction with FAM64A may target PICALM to the nucleus in some cells.By similarity

GO - Cellular componenti

  1. AP-2 adaptor complex Source: Ensembl
  2. coated pit Source: UniProtKB
  3. Golgi apparatus Source: UniProtKB-KW
  4. intracellular Source: BHF-UCL
  5. neurofibrillary tangle Source: Alzheimers_University_of_Toronto
  6. neuronal cell body Source: Alzheimers_University_of_Toronto
  7. nucleus Source: UniProtKB-KW
  8. vesicle Source: Alzheimers_University_of_Toronto
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 660659Phosphatidylinositol-binding clathrin assembly proteinPRO_0000187063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ7M6Y3.
PaxDbiQ7M6Y3.
PRIDEiQ7M6Y3.

PTM databases

PhosphoSiteiQ7M6Y3.

Expressioni

Tissue specificityi

Skins and livers of 1-week-old mice.1 Publication

Gene expression databases

BgeeiQ7M6Y3.
GenevestigatoriQ7M6Y3.

Interactioni

Subunit structurei

Binds clathrin and phosphatidylinositol 4,5-bisphosphate. Interacts with FAM64A; this interaction may change the subcellular location into the nucleus.By similarity

Protein-protein interaction databases

BioGridi231417. 2 interactions.
IntActiQ7M6Y3. 3 interactions.
MINTiMINT-1850199.
STRINGi10090.ENSMUSP00000051092.

Structurei

3D structure databases

ProteinModelPortaliQ7M6Y3.
SMRiQ7M6Y3. Positions 20-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 145132ENTHCuratedPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 29474Interaction with FAM64ABy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG267212.
GeneTreeiENSGT00390000008805.
HOVERGENiHBG049391.
InParanoidiQ7M6Y3.
OMAiWNAATMA.
OrthoDBiEOG76QFM8.
PhylomeDBiQ7M6Y3.
TreeFamiTF314861.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_Pinositid-bd_GAT-like.
IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
[Graphical view]
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 1Curated (identifier: Q7M6Y3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN
60 70 80 90 100
EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR
160 170 180 190 200
GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL
210 220 230 240 250
FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF
260 270 280 290 300
LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT
310 320 330 340 350
TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP
360 370 380 390 400
HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT
410 420 430 440 450
FHPSVHAMSA APQGASTWGD PFSATLDAVE DAIPSLNPFL TKSSGDVHLP
460 470 480 490 500
IASDVSTFTT RTPTHEMFVG FSPSPVAQPH SSAGLNVDFE SVFGNKSTNV
510 520 530 540 550
AVDSGGFDEL GGLLKPTVAS QNQSLPVAKL PPNKLVSDDL DSSLANLVGN
560 570 580 590 600
LGIGNGTTKN DVSWSQPGEK KLTGGSNWQP KVAPTTAWSA ATMNGMHFPQ
610 620 630 640 650
YAPPVMAYPA TTPTGMIGYG IPPQMGSVPV MTQPTLIYSQ PVMRPPNPFG
660
PVSGAQIQFM
Length:660
Mass (Da):71,543
Last modified:December 15, 2003 - v1
Checksum:i7FB206508281BCDF
GO
Isoform 2Curated (identifier: Q7M6Y3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.
     506-510: Missing.
     594-601: Missing.

Show »
Length:597
Mass (Da):64,648
Checksum:i825D7F7B7154DD8A
GO
Isoform 3Curated (identifier: Q7M6Y3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.
     506-510: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:605
Mass (Da):65,623
Checksum:iC365144C975C2B50
GO
Isoform 4Curated (identifier: Q7M6Y3-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     420-469: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:610
Mass (Da):66,184
Checksum:i86E8189B6C05F005
GO
Isoform 5Curated (identifier: Q7M6Y3-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     506-510: Missing.

Show »
Length:655
Mass (Da):70,982
Checksum:iF729CEF5D3D82781
GO
Isoform 6Curated (identifier: Q7M6Y3-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     594-601: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:652
Mass (Da):70,568
Checksum:i0CEEEEA9225CC446
GO

Sequence cautioni

The sequence BAC39454.2 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti522 – 5221N → S in AAH21491. (PubMed:15489334)Curated
Sequence conflicti522 – 5221N → S in AAH23843. (PubMed:15489334)Curated
Sequence conflicti522 – 5221N → S in AAH25566. (PubMed:15489334)Curated
Sequence conflicti522 – 5221N → S in AAH57683. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei420 – 46950Missing in isoform 2, isoform 3 and isoform 4. 2 PublicationsVSP_050684Add
BLAST
Alternative sequencei506 – 5105Missing in isoform 2, isoform 3 and isoform 5. 2 PublicationsVSP_050685
Alternative sequencei594 – 6018Missing in isoform 2 and isoform 6. 2 PublicationsVSP_050686

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY206701 mRNA. Translation: AAO17153.1.
BK001028 mRNA. Translation: DAA01470.1.
BC011470 mRNA. Translation: AAH11470.1.
BC021491 mRNA. Translation: AAH21491.1.
BC023843 mRNA. Translation: AAH23843.1.
BC025566 mRNA. Translation: AAH25566.1.
BC027116 mRNA. Translation: AAH27116.1.
BC057683 mRNA. Translation: AAH57683.1.
AK085472 mRNA. Translation: BAC39454.2. Different initiation.
AK162360 mRNA. Translation: BAE36872.1.
CCDSiCCDS52307.1. [Q7M6Y3-1]
RefSeqiNP_001239449.1. NM_001252520.1. [Q7M6Y3-5]
NP_001239450.1. NM_001252521.1. [Q7M6Y3-6]
NP_001239451.1. NM_001252522.1. [Q7M6Y3-4]
NP_001239452.1. NM_001252523.1. [Q7M6Y3-3]
NP_001239453.1. NM_001252524.1. [Q7M6Y3-2]
NP_666306.2. NM_146194.4. [Q7M6Y3-1]
UniGeneiMm.235175.
Mm.393563.

Genome annotation databases

EnsembliENSMUST00000049537; ENSMUSP00000051092; ENSMUSG00000039361. [Q7M6Y3-1]
GeneIDi233489.
KEGGimmu:233489.
UCSCiuc009igq.2. mouse. [Q7M6Y3-1]
uc009igr.2. mouse. [Q7M6Y3-5]
uc009igs.2. mouse. [Q7M6Y3-6]
uc009igt.2. mouse. [Q7M6Y3-4]
uc009igu.2. mouse. [Q7M6Y3-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY206701 mRNA. Translation: AAO17153.1 .
BK001028 mRNA. Translation: DAA01470.1 .
BC011470 mRNA. Translation: AAH11470.1 .
BC021491 mRNA. Translation: AAH21491.1 .
BC023843 mRNA. Translation: AAH23843.1 .
BC025566 mRNA. Translation: AAH25566.1 .
BC027116 mRNA. Translation: AAH27116.1 .
BC057683 mRNA. Translation: AAH57683.1 .
AK085472 mRNA. Translation: BAC39454.2 . Different initiation.
AK162360 mRNA. Translation: BAE36872.1 .
CCDSi CCDS52307.1. [Q7M6Y3-1 ]
RefSeqi NP_001239449.1. NM_001252520.1. [Q7M6Y3-5 ]
NP_001239450.1. NM_001252521.1. [Q7M6Y3-6 ]
NP_001239451.1. NM_001252522.1. [Q7M6Y3-4 ]
NP_001239452.1. NM_001252523.1. [Q7M6Y3-3 ]
NP_001239453.1. NM_001252524.1. [Q7M6Y3-2 ]
NP_666306.2. NM_146194.4. [Q7M6Y3-1 ]
UniGenei Mm.235175.
Mm.393563.

3D structure databases

ProteinModelPortali Q7M6Y3.
SMRi Q7M6Y3. Positions 20-288.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231417. 2 interactions.
IntActi Q7M6Y3. 3 interactions.
MINTi MINT-1850199.
STRINGi 10090.ENSMUSP00000051092.

PTM databases

PhosphoSitei Q7M6Y3.

Proteomic databases

MaxQBi Q7M6Y3.
PaxDbi Q7M6Y3.
PRIDEi Q7M6Y3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000049537 ; ENSMUSP00000051092 ; ENSMUSG00000039361 . [Q7M6Y3-1 ]
GeneIDi 233489.
KEGGi mmu:233489.
UCSCi uc009igq.2. mouse. [Q7M6Y3-1 ]
uc009igr.2. mouse. [Q7M6Y3-5 ]
uc009igs.2. mouse. [Q7M6Y3-6 ]
uc009igt.2. mouse. [Q7M6Y3-4 ]
uc009igu.2. mouse. [Q7M6Y3-3 ]

Organism-specific databases

CTDi 8301.
MGIi MGI:2385902. Picalm.

Phylogenomic databases

eggNOGi NOG267212.
GeneTreei ENSGT00390000008805.
HOVERGENi HBG049391.
InParanoidi Q7M6Y3.
OMAi WNAATMA.
OrthoDBi EOG76QFM8.
PhylomeDBi Q7M6Y3.
TreeFami TF314861.

Miscellaneous databases

ChiTaRSi PICALM. mouse.
NextBioi 381725.
PROi Q7M6Y3.
SOURCEi Search...

Gene expression databases

Bgeei Q7M6Y3.
Genevestigatori Q7M6Y3.

Family and domain databases

Gene3Di 1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProi IPR011417. ANTH_dom.
IPR014712. Clathrin_Pinositid-bd_GAT-like.
IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
[Graphical view ]
Pfami PF07651. ANTH. 1 hit.
[Graphical view ]
SMARTi SM00273. ENTH. 1 hit.
[Graphical view ]
SUPFAMi SSF48464. SSF48464. 1 hit.
PROSITEi PS50942. ENTH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities in fit1 mice."
    Klebig M.L., Wall M.D., Potter M.D., Rowe E.L., Carpenter D.A., Rinchik E.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:8360-8365(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Strain: BALB/cRlImported.
    Tissue: LiverImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3; 4; 5 AND 6).
    Strain: Czech II and FVB/NImported.
    Tissue: Mammary tumor and Retina.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 143-660 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Epididymis and Kidney.
  4. "Mutations in the murine fitness 1 gene result in defective hematopoiesis."
    Potter M.D., Shinpock S.G., Popp R.A., Godfrey V., Carpenter D.A., Bernstein A., Johnson D.K., Rinchik E.M.
    Blood 90:1850-1857(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPICAL_MOUSE
AccessioniPrimary (citable) accession number: Q7M6Y3
Secondary accession number(s): Q3TS04
, Q811P1, Q8BUF6, Q8CIH8, Q8R0A9, Q8R3E1, Q8VDN5, Q921L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3