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Q7M4T0

- NEG1_NEUCR

UniProt

Q7M4T0 - NEG1_NEUCR

Protein

Endo-1,6-beta-D-glucanase

Gene

neg-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Partially degrades N.crassa cell wall beta-D-glucan, liberating small amounts of oligosaccharides.

    Catalytic activityi

    Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei225 – 2251Proton donorSequence Analysis
    Active sitei321 – 3211NucleophileBy similarity

    GO - Molecular functioni

    1. glucan endo-1,6-beta-glucosidase activity Source: UniProtKB-EC
    2. glucosylceramidase activity Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. sphingolipid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.75. 3627.

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.
    mycoCLAPiBGN30A_NEUCR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,6-beta-D-glucanase (EC:3.2.1.75)
    Alternative name(s):
    Beta-1,6-glucanase Neg1
    Glucan endo-1,6-beta-glucosidase
    Gene namesi
    Name:neg-1
    ORF Names:29E8.200, NCU04395
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 4, Linkage Group IV

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17171 PublicationAdd
    BLAST
    Chaini18 – 480463Endo-1,6-beta-D-glucanasePRO_0000012180Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU04395.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7M4T0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5520.
    HOGENOMiHOG000159183.
    KOiK01201.
    OMAiDINILIW.
    OrthoDBiEOG7966S1.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    PRINTSiPR00843. GLHYDRLASE30.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q7M4T0-1 [UniParc]FASTAAdd to Basket

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    MYPPALTLLL TPGLVAAAIQ PQAYASSADG RYKLSSYSAP VRGTGTPGSN    50
    STWKLTIDDT PSGRKQTIKG FGAAVTDSTV SVFNALPSAQ RTALLNTLMT 100
    TAGANFAMMR HTIASSDLSA NPAYSYDDSN GQTDLSLSNF NLGGRGNAMA 150
    SLLAEMRRLQ PGLTILGSPW SPPGWMKLNR AIQGTTVNNN LDHAYASQFA 200
    QYFVKYLQAY QAKGATIDAI TIQNEPLNSR AQMPTMYIYA DEAGDLIQNN 250
    IGPALRNAGL DTKIWAYDHN TDQPSYPSTV LSRAGGYVPA VAWHCYASSL 300
    DWSVLTTFHN AHPGVEQYMT ECWTSAKQPT PWNWAASFTM GPLQNWASGV 350
    TAWVLGTDTN DGPHLTGSDA CDKCTGLVTV DAAAGTYNLR GDYYMMAQFS 400
    KFMKKGAVVM SGTGSWTYGD GSGLESVAAT NADDGSRVVV IENKFGNEIY 450
    VTVEAKSGEV WSGLVYRNSV VTWVLPAAGA 480
    Length:480
    Mass (Da):51,353
    Last modified:February 1, 2005 - v2
    Checksum:i9183B780A3B491F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti101 – 1011T → P in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti131 – 1311G → D in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti144 – 1441G → D in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti181 – 1811A → V in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti216 – 2161T → K in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti278 – 2781S → A in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti303 – 3031S → T in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti327 – 3271K → R in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti379 – 3791T → M in BAB91213. (PubMed:12162562)Curated
    Sequence conflicti382 – 3821A → T in BAB91213. (PubMed:12162562)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073820 mRNA. Translation: BAB91213.1.
    BX908809 Genomic DNA. Translation: CAF06053.1.
    CM002239 Genomic DNA. Translation: EAA28236.1.
    PIRiJC7866.
    RefSeqiXP_957472.1. XM_952379.1.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000005204; EFNCRP00000005200; EFNCRG00000005197.
    GeneIDi3873626.
    KEGGincr:NCU04395.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073820 mRNA. Translation: BAB91213.1 .
    BX908809 Genomic DNA. Translation: CAF06053.1 .
    CM002239 Genomic DNA. Translation: EAA28236.1 .
    PIRi JC7866.
    RefSeqi XP_957472.1. XM_952379.1.

    3D structure databases

    ProteinModelPortali Q7M4T0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU04395.1.

    Protein family/group databases

    CAZyi GH30. Glycoside Hydrolase Family 30.
    mycoCLAPi BGN30A_NEUCR.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000005204 ; EFNCRP00000005200 ; EFNCRG00000005197 .
    GeneIDi 3873626.
    KEGGi ncr:NCU04395.

    Phylogenomic databases

    eggNOGi COG5520.
    HOGENOMi HOG000159183.
    KOi K01201.
    OMAi DINILIW.
    OrthoDBi EOG7966S1.

    Enzyme and pathway databases

    BRENDAi 3.2.1.75. 3627.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR11069. PTHR11069. 1 hit.
    Pfami PF02055. Glyco_hydro_30. 1 hit.
    [Graphical view ]
    PRINTSi PR00843. GLHYDRLASE30.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of an endo-1,6-beta-D-glucanase gene (neg1) from Neurospora crassa."
      Oyama S., Yamagata Y., Abe K., Nakajima T.
      Biosci. Biotechnol. Biochem. 66:1378-1381(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-35; 243-257; 323-330 AND 426-435, CHARACTERIZATION.
      Strain: ATCC 10336 / CBS 304.59 / FGSC 1757 / NBRC 6068 / IMI 53238.
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiNEG1_NEUCR
    AccessioniPrimary (citable) accession number: Q7M4T0
    Secondary accession number(s): Q7RZ78, Q8NKJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3