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Q7M4T0 (NEG1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endo-1,6-beta-D-glucanase

EC=3.2.1.75
Alternative name(s):
Beta-1,6-glucanase Neg1
Glucan endo-1,6-beta-glucosidase
Gene names
Name:neg-1
ORF Names:29E8.200, NCU04395
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Partially degrades N.crassa cell wall beta-D-glucan, liberating small amounts of oligosaccharides.

Catalytic activity

Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.

Subcellular location

Secreted.

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.1
Chain18 – 480463Endo-1,6-beta-D-glucanase
PRO_0000012180

Sites

Active site2251Proton donor Potential
Active site3211Nucleophile By similarity

Amino acid modifications

Glycosylation501N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1011T → P in BAB91213. Ref.1
Sequence conflict1311G → D in BAB91213. Ref.1
Sequence conflict1441G → D in BAB91213. Ref.1
Sequence conflict1811A → V in BAB91213. Ref.1
Sequence conflict2161T → K in BAB91213. Ref.1
Sequence conflict2781S → A in BAB91213. Ref.1
Sequence conflict3031S → T in BAB91213. Ref.1
Sequence conflict3271K → R in BAB91213. Ref.1
Sequence conflict3791T → M in BAB91213. Ref.1
Sequence conflict3821A → T in BAB91213. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q7M4T0 [UniParc].

Last modified February 1, 2005. Version 2.
Checksum: 9183B780A3B491F8

FASTA48051,353
        10         20         30         40         50         60 
MYPPALTLLL TPGLVAAAIQ PQAYASSADG RYKLSSYSAP VRGTGTPGSN STWKLTIDDT 

        70         80         90        100        110        120 
PSGRKQTIKG FGAAVTDSTV SVFNALPSAQ RTALLNTLMT TAGANFAMMR HTIASSDLSA 

       130        140        150        160        170        180 
NPAYSYDDSN GQTDLSLSNF NLGGRGNAMA SLLAEMRRLQ PGLTILGSPW SPPGWMKLNR 

       190        200        210        220        230        240 
AIQGTTVNNN LDHAYASQFA QYFVKYLQAY QAKGATIDAI TIQNEPLNSR AQMPTMYIYA 

       250        260        270        280        290        300 
DEAGDLIQNN IGPALRNAGL DTKIWAYDHN TDQPSYPSTV LSRAGGYVPA VAWHCYASSL 

       310        320        330        340        350        360 
DWSVLTTFHN AHPGVEQYMT ECWTSAKQPT PWNWAASFTM GPLQNWASGV TAWVLGTDTN 

       370        380        390        400        410        420 
DGPHLTGSDA CDKCTGLVTV DAAAGTYNLR GDYYMMAQFS KFMKKGAVVM SGTGSWTYGD 

       430        440        450        460        470        480 
GSGLESVAAT NADDGSRVVV IENKFGNEIY VTVEAKSGEV WSGLVYRNSV VTWVLPAAGA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of an endo-1,6-beta-D-glucanase gene (neg1) from Neurospora crassa."
Oyama S., Yamagata Y., Abe K., Nakajima T.
Biosci. Biotechnol. Biochem. 66:1378-1381(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-35; 243-257; 323-330 AND 426-435, CHARACTERIZATION.
Strain: ATCC 10336 / CBS 304.59 / FGSC 1757 / NBRC 6068 / IMI 53238.
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB073820 mRNA. Translation: BAB91213.1.
BX908809 Genomic DNA. Translation: CAF06053.1.
CM002239 Genomic DNA. Translation: EAA28236.1.
PIRJC7866.
RefSeqXP_957472.1. XM_952379.1.

3D structure databases

ProteinModelPortalQ7M4T0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU04395.1.

Protein family/group databases

CAZyGH30. Glycoside Hydrolase Family 30.
mycoCLAPBGN30A_NEUCR.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000005204; EFNCRP00000005200; EFNCRG00000005197.
GeneID3873626.
KEGGncr:NCU04395.

Phylogenomic databases

eggNOGCOG5520.
HOGENOMHOG000159183.
KOK01201.
OMALMDANGF.
OrthoDBEOG7966S1.

Enzyme and pathway databases

BRENDA3.2.1.75. 3627.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSPR00843. GLHYDRLASE30.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNEG1_NEUCR
AccessionPrimary (citable) accession number: Q7M4T0
Secondary accession number(s): Q7RZ78, Q8NKJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: February 19, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries