Q7M4I5 (PA2_APIDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phospholipase A2 Short name=PLA2 EC=3.1.1.4 Alternative name(s): Phosphatidylcholine 2-acylhydrolase Allergen=Api d 1
Organism	Apis dorsata (Giant honeybee)
Taxonomic identifier	7462 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apinae › Apini › Apis

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human By similarity.
Sequence similarities	Belongs to the phospholipase A2 family. Group III subfamily.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Disease	Allergen
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Phospholipase A2		PRO_0000161723
Sites
	Active site	34	1	By similarity
	Active site	64	1	By similarity
	Metal binding	8	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	10	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	12	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	35	1	Calcium By similarity
Amino acid modifications
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Disulfide bond	9 ↔ 31		By similarity
	Disulfide bond	30 ↔ 70		By similarity
	Disulfide bond	37 ↔ 63		By similarity
	Disulfide bond	61 ↔ 95		By similarity
	Disulfide bond	105 ↔ 113		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7M4I5 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 2843633DA79ED27B	FASTA	134	15,246
	10 20 30 40 50 60 IIYPGTLWCG HGNVSSSPDE LGRFKHTDSC CRSHDMCPDV MSAGESKHGL TNTASHTRLS 70 80 90 100 110 120 CDCDDKFYDC LKNSSDTISS YFVGEMYFNI LDTKCYKLEH PVTGCGKRTE GRCLNYTVDK 130 SKPKVYQWFD LRKY

References

[1]	Hoffman D.R., Schmidt J.O. Submitted (JUL-1999) to the PIR data bank Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	B59055.
3D structure databases
ProteinModelPortal	Q7M4I5.
SMR	Q7M4I5. Positions 1-134.
ModBase	Search...
Protein family/group databases
Allergome	1282. Api d 1. 3085. Api d 1.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.90.10. 1 hit.
InterPro	IPR001211. PLipase_A2. IPR013090. PLipase_A2_AS. IPR016090. PLipase_A2_dom. [Graphical view]
PANTHER	PTHR12253. PTHR12253. 1 hit.
Pfam	PF05826. Phospholip_A2_2. 1 hit. [Graphical view]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. False negative. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA2_APIDO

Accession

Primary (citable) accession number: Q7M4I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)