Q7M485 (A1X1_LOXRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sphingomyelin phosphodiesterase D LrSicTox-alphaI-1 Alternative name(s): Dermonecrotic protein Mammalian toxin Sphingomyelin phosphodiesterase D Short name=SMD Short name=SMase D Short name=Sphingomyelinase D EC=3.1.4.41
Organism	Loxosceles reclusa (Brown recluse spider)
Taxonomic identifier	6921 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Haplogynae › Sicariidae › Loxosceles

Protein attributes

Sequence length	35 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolysis of sphingomyelin. Induces complement-dependent hemolysis, dermonecrosis, blood vessel permeability and platelet aggregation By similarity. Ref.2
Catalytic activity	Sphingomyelin + H2O = ceramide phosphate + choline.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Contains 2 disulfide bonds By similarity.
Sequence similarities	Belongs to the SicTox family. Alpha subfamily. Contains 1 GDPD domain.

Ontologies

Keywords
Biological process	Cytolysis Dermonecrosis Hemolysis
Cellular component	Secreted
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW lipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›35	›35	Sphingomyelin phosphodiesterase D LrSicTox-alphaI-1		PRO_0000087677
Sites
	Active site	11	1	Nucleophile By similarity
	Metal binding	33	1	Magnesium By similarity
Experimental info
	Non-terminal residue	35	1

Sequences

	Sequence		Length	Mass (Da)
	Q7M485 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: B6EFE750E46C1AFB	FASTA	35	3,905
	10 20 30 ANKRPVWIMG HMVNAVYQID EFVNLGANSI DTDVS

References

[1]	"Screening a brown recluse spider genomic library for the gene coding for the mammalian toxin using an oligonucleotide probe." Cisar C.R., Fox J.W., Geren C.R. Toxicon 27:37-37(1989) Cited for: PROTEIN SEQUENCE.
[2]	"Antigenic cross-reactivity of venoms from medically important north american Loxosceles spider species." Gomez H.F., Miller M.J., Waggener M.W., Lankford H.A., Warren J.S. Toxicon 39:817-824(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
PIR	A38107.
3D structure databases
ProteinModelPortal	Q7M485.
SMR	Q7M485. Positions 2-35.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ArachnoServer	AS000160. Sphingomyelinase D (LrSicTox1) (N-terminal fragment).
Family and domain databases
Gene3D	3.20.20.190. 1 hit.
InterPro	IPR017946. PLC-like_Pdiesterase_TIM-brl. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1X1_LOXRE

Accession

Primary (citable) accession number: Q7M485

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	December 15, 2003
Last modified:	March 6, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program