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Protein

Chymotrypsin-C

Gene

CTRC

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has chymotrypsin-type protease activity and hypocalcemic activity.By similarity

Catalytic activityi

Preferential cleavage: Leu-|-Xaa, Tyr-|-Xaa, Phe-|-Xaa, Met-|-Xaa, Trp-|-Xaa, Gln-|-Xaa, Asn-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741Charge relay systemBy similarity
Active sitei121 – 1211Charge relay systemBy similarity
Active sitei216 – 2161Charge relay systemBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-BTA-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Protein family/group databases

MEROPSiS01.157.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-C (EC:3.4.21.2)
Alternative name(s):
bPTLP
Gene namesi
Name:CTRC
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Add
BLAST
Propeptidei17 – 2913Activation peptide1 PublicationPRO_0000288614Add
BLAST
Chaini30 – 268239Chymotrypsin-CPRO_0000288615Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi17 ↔ 141PROSITE-ProRule annotation
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence analysis
Disulfide bondi59 ↔ 75PROSITE-ProRule annotation
Disulfide bondi155 ↔ 222PROSITE-ProRule annotation
Disulfide bondi186 ↔ 202PROSITE-ProRule annotation
Disulfide bondi212 ↔ 243PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ7M3E1.

Expressioni

Tissue specificityi

Pancreas.2 Publications

Interactioni

Subunit structurei

Monomer. The zymogen is secreted as a ternary complex composed of procarboxypeptidase A, chymotrypsinogen C and proproteinase E.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000033942.

Structurei

Secondary structure

1
268
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 294Combined sources
Beta strandi44 – 518Combined sources
Beta strandi54 – 6512Combined sources
Beta strandi68 – 714Combined sources
Helixi73 – 753Combined sources
Beta strandi82 – 876Combined sources
Beta strandi100 – 10910Combined sources
Turni115 – 1184Combined sources
Beta strandi123 – 1297Combined sources
Beta strandi154 – 1574Combined sources
Beta strandi177 – 1793Combined sources
Helixi183 – 1864Combined sources
Turni189 – 1957Combined sources
Beta strandi200 – 2034Combined sources
Beta strandi219 – 2257Combined sources
Beta strandi227 – 23711Combined sources
Beta strandi250 – 2545Combined sources
Helixi255 – 2584Combined sources
Helixi259 – 2657Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PYTX-ray2.35D17-268[»]
ProteinModelPortaliQ7M3E1.
SMRiQ7M3E1. Positions 17-268.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7M3E1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 268239Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ7M3E1.
KOiK01311.
OMAiESDTIQA.
OrthoDBiEOG75B84T.
TreeFamiTF330455.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7M3E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGITVFTTF LAYASSCGAP IFQPNLSARV VGGEDAIPHS WPWQISLQYL
60 70 80 90 100
RDNTWRHTCG GTLITPNHVL TAAHCISNTL TYRVALGKNN LEVEDEAGSL
110 120 130 140 150
YVGVDTIFVH EKWNSFLVRN DIALIKLAET VELSDTIQVA CLPEEGSLLP
160 170 180 190 200
QDYPCFVTGW GRLYTNGPIA AELQQGLQPV VDYATCSQRD WWGTTVKETM
210 220 230 240 250
VCAGGDGVIS ACNGDSGGPL NCQAENGNWD VRGIVSFGSG LSCNTFKKPT
260
VFTRVSAYID WINQKLQL
Length:268
Mass (Da):29,255
Last modified:May 29, 2007 - v2
Checksum:i7B006994F91A04D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134798 mRNA. Translation: AAI34799.1.
BC142035 mRNA. Translation: AAI42036.1.
CK771392 mRNA. No translation available.
PIRiPU0036.
RefSeqiNP_001092435.1. NM_001098965.1.
UniGeneiBt.45270.

Genome annotation databases

EnsembliENSBTAT00000034040; ENSBTAP00000033942; ENSBTAG00000024497.
GeneIDi514047.
KEGGibta:514047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC134798 mRNA. Translation: AAI34799.1.
BC142035 mRNA. Translation: AAI42036.1.
CK771392 mRNA. No translation available.
PIRiPU0036.
RefSeqiNP_001092435.1. NM_001098965.1.
UniGeneiBt.45270.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PYTX-ray2.35D17-268[»]
ProteinModelPortaliQ7M3E1.
SMRiQ7M3E1. Positions 17-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000033942.

Protein family/group databases

MEROPSiS01.157.

Proteomic databases

PaxDbiQ7M3E1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000034040; ENSBTAP00000033942; ENSBTAG00000024497.
GeneIDi514047.
KEGGibta:514047.

Organism-specific databases

CTDi11330.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ7M3E1.
KOiK01311.
OMAiESDTIQA.
OrthoDBiEOG75B84T.
TreeFamiTF330455.

Enzyme and pathway databases

ReactomeiR-BTA-196741. Cobalamin (Cbl, vitamin B12) transport and metabolism.

Miscellaneous databases

EvolutionaryTraceiQ7M3E1.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal pancreas.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 38-268.
  3. "Purification and characterization of a novel serine proteinase from the microsomal fraction of bovine pancreas."
    Tsuji A., Edazawa K., Sakiyama K., Nagata K., Sasaki Y., Nagamune H., Matsuda Y.
    J. Biochem. 119:100-105(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-52.
    Tissue: Pancreas.
  4. "The three-dimensional structure of the native ternary complex of bovine pancreatic procarboxypeptidase A with proproteinase E and chymotrypsinogen C."
    Gomis-Rueth F.-X., Gomez M., Bode W., Huber R., Aviles F.X.
    EMBO J. 14:4387-4394(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 17-268 OF COMPLEX WITH CPA1 AND PPE, DISULFIDE BONDS, TISSUE SPECIFICITY.
  5. "Crystal structure of an oligomer of proteolytic zymogens: detailed conformational analysis of the bovine ternary complex and implications for their activation."
    Gomis-Ruth F.-X., Gomez-Ortiz M., Vendrell J., Ventura S., Bode W., Huber R., Aviles F.X.
    J. Mol. Biol. 269:861-880(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 17-268 OF COMPLEX WITH CPA1 AND PPE, DISULFIDE BONDS, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCTRC_BOVIN
AccessioniPrimary (citable) accession number: Q7M3E1
Secondary accession number(s): A5PJB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: June 8, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.