Reviewed, UniProtKB/Swiss-Prot Q7M3E1 (CTRC_BOVIN)

Names and origin

Protein names

Recommended name:
    Chymotrypsin-C
    EC=3.4.21.2
Alternative name(s):
    bPTLP

Gene names

Name:

CTRC

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has chymotrypsin-type protease activity and hypocalcemic activity By similarity.
Catalytic activity	Preferential cleavage: Leu-|-Xaa, Tyr-|-Xaa, Phe-|-Xaa, Met-|-Xaa, Trp-|-Xaa, Gln-|-Xaa, Asn-|-Xaa.
Subunit structure	Monomer. The zymogen is secreted as a ternary complex composed of procarboxypeptidase A, chymotrypsinogen C and proproteinase E.
Subcellular location	Secreted › extracellular space By similarity.
Tissue specificity	Pancreas. Ref.4 Ref.5
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16
	Propeptide	17 – 29	13	Activation peptide Ref.3		PRO_0000288614
	Chain	30 – 268	239	Chymotrypsin-C		PRO_0000288615
Regions
	Domain	30 – 268	239	Peptidase S1
Sites
	Active site	74	1	Charge relay system By similarity
	Active site	121	1	Charge relay system By similarity
	Active site	216	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Disulfide bond	17 ↔ 141		By similarity
	Disulfide bond	59 ↔ 75		By similarity
	Disulfide bond	155 ↔ 222		By similarity
	Disulfide bond	186 ↔ 202		By similarity
	Disulfide bond	212 ↔ 243		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7M3E1-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 7B006994F91A04D8	FASTA	268	29,255
	10 20 30 40 50 60 MLGITVFTTF LAYASSCGAP IFQPNLSARV VGGEDAIPHS WPWQISLQYL RDNTWRHTCG 70 80 90 100 110 120 GTLITPNHVL TAAHCISNTL TYRVALGKNN LEVEDEAGSL YVGVDTIFVH EKWNSFLVRN 130 140 150 160 170 180 DIALIKLAET VELSDTIQVA CLPEEGSLLP QDYPCFVTGW GRLYTNGPIA AELQQGLQPV 190 200 210 220 230 240 VDYATCSQRD WWGTTVKETM VCAGGDGVIS ACNGDSGGPL NCQAENGNWD VRGIVSFGSG 250 260 LSCNTFKKPT VFTRVSAYID WINQKLQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pancreas.
[2]	"Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle." Smith T.P.L., Grosse W.M., Freking B.A., Roberts A.J., Stone R.T., Casas E., Wray J.E., White J., Cho J., Fahrenkrug S.C., Bennett G.L., Heaton M.P., Laegreid W.W., Rohrer G.A., Chitko-McKown C.G., Pertea G., Holt I., Karamycheva S. , Liang F., Quackenbush J., Keele J.W. Genome Res. 11:626-630(2001) [PubMed: 11282978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 38-268.
[3]	"Purification and characterization of a novel serine proteinase from the microsomal fraction of bovine pancreas." Tsuji A., Edazawa K., Sakiyama K., Nagata K., Sasaki Y., Nagamune H., Matsuda Y. J. Biochem. 119:100-105(1996) [PubMed: 8907182] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-52. Tissue: Pancreas.
[4]	"The three-dimensional structure of the native ternary complex of bovine pancreatic procarboxypeptidase A with proproteinase E and chymotrypsinogen C." Gomis-Rueth F.-X., Gomez M., Bode W., Huber R., Aviles F.X. EMBO J. 14:4387-4394(1995) [PubMed: 7556081] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 17-268 OF COMPLEX WITH CPA1 AND PPE, DISULFIDE BONDS, TISSUE SPECIFICITY.
[5]	"Crystal structure of an oligomer of proteolytic zymogens: detailed conformational analysis of the bovine ternary complex and implications for their activation." Gomis-Ruth F.-X., Gomez-Ortiz M., Vendrell J., Ventura S., Bode W., Huber R., Aviles F.X. J. Mol. Biol. 269:861-880(1997) [PubMed: 9223647] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 17-268 OF COMPLEX WITH CPA1 AND PPE, DISULFIDE BONDS, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BC134798 mRNA. Translation: AAI34799.1.
BC142035 mRNA. Translation: AAI42036.1.
CK771392 mRNA. No translation available.

IPI

IPI00703602.

PIR

PU0036.

RefSeq

NP_001092435.1.

UniGene

Bt.45270

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PYT	X-ray	2.35	D	17-264	[»]

ModBase

Search...

Protein family/group databases

MEROPS

S01.157.

Genome annotation databases

Ensembl

ENSBTAG00000024497. Bos taurus. [Contig view]

GeneID

514047.

KEGG

bta:514047.

Phylogenomic databases

HOVERGEN

Q7M3E1.

OMA

Q7M3E1. CISNTRT.

Enzyme and pathway databases

BRENDA

3.4.21.2. 251.

Family and domain databases

InterPro

IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CTRC_BOVIN

Accession

Primary (citable) accession number: Q7M3E1
Secondary accession number(s): A5PJB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)