Reviewed, UniProtKB/Swiss-Prot Q7M1Z2 (RIP9_SAPOF)

Names and origin

Protein names

Recommended name:
    Ribosome-inactivating protein saporin-9
      Short name=SAP-9
      Short name=SO-9
    EC=3.2.2.22
Alternative name(s):
    rRNA N-glycosidase

Gene names

Name:

SAP9

Organism

Saponaria officinalis (Common soapwort)

Taxonomic identifier

3572 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Saponaria

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells By similarity. UniProtKB P20656
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. UniProtKB P20656
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Mass spectrometry	Molecular mass is 28495±2 Da from positions 1 - 253. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Ribosome-inactivating protein saporin-9		PRO_0000253946
Sites
	Active site	176	1	By similarity UniProtKB P33186

Sequences

	Sequence		Length	Mass (Da)
	Q7M1Z2-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 22915D5494842389	FASTA	253	28,494
	10 20 30 40 50 60 VTSITLDLVN PTAGQYSSFV DKIRNNVKDP NLKYGGTDIA VIGPPSKDKF LRINFQSSRG 70 80 90 100 110 120 TVSLGLKRDN LYVVAYLAMD NTNVNRAYYF RSEITSAELT ALFPEATAAN HKALEYTEDY 130 140 150 160 170 180 HSIEKNAQIT EGDKSRKELG LGINLLSSTM DTVNKKVRVV KNEARFLLIA IQMTAEAVRF 190 200 210 220 230 240 RYIQNLVTKN FPNKFNSENK VIKFEVNWKK ISTAIHGDAK NGVFNKDYDF GFGKVRLVKD 250 LQMGLLMHLG KPK

References

[1]

"Reliable sequence determination of ribosome-inactivating proteins by combining electrospray mass spectrometry and Edman degradation." Di Maro A., Ferranti P., Mastronicola M., Polito L., Bolognesi A., Stirpe F., Malorni A., Parente A. J. Mass Spectrom. 36:38-46(2001) [PubMed: 11180645] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Seed.

Cross-references

Sequence databases
PIR	A58923.
3D structure databases
HSSP	HSSP built from PDB template 1QI7 based on UniProtKB P27559.
SMR	Q7M1Z2. Positions 1-253.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 39707.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP9_SAPOF

Accession

Primary (citable) accession number: Q7M1Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	December 15, 2003
Last modified:	November 24, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)