Q7M1E7 (PGLR2_CHAOB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Polygalacturonase Short name=PG EC=3.2.1.15 Alternative name(s): Major pollen allergen Cha o 2 Pectinase Allergen=Cha o 2
Organism	Chamaecyparis obtusa (Hinoki false-cypress)
Taxonomic identifier	13415 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Cupressaceae › Chamaecyparis

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted. Secreted › cell wall.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Fruit ripening
Cellular component	Cell wall Secreted
Disease	Allergen
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 50	28			PRO_0000024814
	Chain	51 – 514	464	Polygalacturonase		PRO_0000024815
Regions
	Repeat	214 – 240	27	PbH1 1
	Repeat	241 – 262	22	PbH1 2
	Repeat	264 – 284	21	PbH1 3
	Repeat	294 – 315	22	PbH1 4
	Repeat	323 – 344	22	PbH1 5
	Repeat	357 – 384	28	PbH1 6
Sites
	Active site	255	1	Proton donor By similarity
	Active site	278	1	By similarity
Amino acid modifications
	Glycosylation	266	1	N-linked (GlcNAc...) Potential
	Glycosylation	397	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7M1E7 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 68B62DEBDD59C1EC	FASTA	514	56,073
	10 20 30 40 50 60 MGMKFMAAVA FLALQLIVMA AAEDQSAQIM LDSDIEQYLR SNRSLKKLVH SRHDAATVFN 70 80 90 100 110 120 VEQYGAVGDG KHDSTEAFAT TWNAACKKAS AVLLVPANKK FFVNNLVFRG PCQPHLSFKV 130 140 150 160 170 180 DGTIVAQPDP ARWKNSKIWL QFAQLTDFNL MGTGVIDGQG QQWWAGQCKV VNGRTVCNDR 190 200 210 220 230 240 NRPTAIKIDY SKSVTVKELT LMNSPEFHLV FGECEGVKIQ GLKIKAPRDS PNTDGIDIFA 250 260 270 280 290 300 SKRFHIEKCV IGTGDDCIAI GTGSSNITIK DLICGPGHGI SIGSLGRDNS RAEVSHVHVN 310 320 330 340 350 360 RAKFIDTQNG LRIKTWQGGS GLASYITYEN VEMINSENPI LINQFYCTSA SACQNQRSAV 370 380 390 400 410 420 QIQGVTYKNI HGTSATAAAI QLMCSDSVPC TGIQLSNVSL KLTSGKPASC VDKNARGFYS 430 440 450 460 470 480 GRLIPTCKNL RPGPSPKEFE LQQQPTTVMD ENKGACAKGD STCISLSSSP PNCKNKCKGC 490 500 510 QPCKPKLIIV HPNKPQDYYP QKWVCSCHNK IYNP

References

[1]	"Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen." Mori T., Yokoyama M., Komiyama N., Okano M., Kino K. Biochem. Biophys. Res. Commun. 263:166-171(1999) [PubMed: 10486272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 51-62. Tissue: Pollen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	JC7100.
3D structure databases
ProteinModelPortal	Q7M1E7.
ModBase	Search...
Protein family/group databases
Allergome	198. Cha o 2. 3187. Cha o 2.0101.
CAZy	GH28. Glycoside Hydrolase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR2_CHAOB

Accession

Primary (citable) accession number: Q7M1E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	December 15, 2003
Last modified:	May 31, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program