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Protein

Polygalacturonase

Gene
N/A
Organism
Chamaecyparis obtusa (Hinoki false-cypress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei255 – 2551Proton donorPROSITE-ProRule annotation
Active sitei278 – 2781PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Fruit ripening

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Major pollen allergen Cha o 2
Pectinase
Allergen: Cha o 2
OrganismiChamaecyparis obtusa (Hinoki false-cypress)
Taxonomic identifieri13415 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeChamaecyparis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei198. Cha o 2.
3187. Cha o 2.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Propeptidei23 – 50281 PublicationPRO_0000024814Add
BLAST
Chaini51 – 514464PolygalacturonasePRO_0000024815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi266 – 2661N-linked (GlcNAc...)Sequence analysis
Glycosylationi397 – 3971N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ7M1E7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati214 – 24027PbH1 1Add
BLAST
Repeati241 – 26222PbH1 2Add
BLAST
Repeati264 – 28421PbH1 3Add
BLAST
Repeati294 – 31522PbH1 4Add
BLAST
Repeati323 – 34422PbH1 5Add
BLAST
Repeati357 – 38428PbH1 6Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7M1E7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMKFMAAVA FLALQLIVMA AAEDQSAQIM LDSDIEQYLR SNRSLKKLVH
60 70 80 90 100
SRHDAATVFN VEQYGAVGDG KHDSTEAFAT TWNAACKKAS AVLLVPANKK
110 120 130 140 150
FFVNNLVFRG PCQPHLSFKV DGTIVAQPDP ARWKNSKIWL QFAQLTDFNL
160 170 180 190 200
MGTGVIDGQG QQWWAGQCKV VNGRTVCNDR NRPTAIKIDY SKSVTVKELT
210 220 230 240 250
LMNSPEFHLV FGECEGVKIQ GLKIKAPRDS PNTDGIDIFA SKRFHIEKCV
260 270 280 290 300
IGTGDDCIAI GTGSSNITIK DLICGPGHGI SIGSLGRDNS RAEVSHVHVN
310 320 330 340 350
RAKFIDTQNG LRIKTWQGGS GLASYITYEN VEMINSENPI LINQFYCTSA
360 370 380 390 400
SACQNQRSAV QIQGVTYKNI HGTSATAAAI QLMCSDSVPC TGIQLSNVSL
410 420 430 440 450
KLTSGKPASC VDKNARGFYS GRLIPTCKNL RPGPSPKEFE LQQQPTTVMD
460 470 480 490 500
ENKGACAKGD STCISLSSSP PNCKNKCKGC QPCKPKLIIV HPNKPQDYYP
510
QKWVCSCHNK IYNP
Length:514
Mass (Da):56,073
Last modified:December 15, 2003 - v1
Checksum:i68B62DEBDD59C1EC
GO

Sequence databases

PIRiJC7100.

Cross-referencesi

Sequence databases

PIRiJC7100.

3D structure databases

ProteinModelPortaliQ7M1E7.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei198. Cha o 2.
3187. Cha o 2.0101.
CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen."
    Mori T., Yokoyama M., Komiyama N., Okano M., Kino K.
    Biochem. Biophys. Res. Commun. 263:166-171(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 51-62.
    Tissue: Pollen.

Entry informationi

Entry nameiPGLR2_CHAOB
AccessioniPrimary (citable) accession number: Q7M1E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: December 15, 2003
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.