Reviewed, UniProtKB/Swiss-Prot Q7M135 (LYSC_LYSEN)

Names and origin

Protein names	Recommended name:     Lysyl endopeptidase     EC=3.4.21.50 Alternative name(s):     Lys-C
Organism	Lysobacter enzymogenes
Taxonomic identifier	69 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Lysobacter

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Highly specific endopeptidase that hydrolyzes lysyl bonds including the Lys-Pro bond.
Catalytic activity	Preferential cleavage: Lys-|-Xaa, including Lys-|-Pro.
Subcellular location	Secreted.
Biotechnological use	Sold under the name 'Endoproteinase Lys-C' by Roche.
Sequence similarities	Belongs to the peptidase S1 family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Lysyl endopeptidase		PRO_0000093854
Sites
	Active site	57	1	Charge relay system By similarity
	Active site	113	1	Charge relay system By similarity
	Active site	194	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	6 ↔ 216		By similarity
	Disulfide bond	12 ↔ 80		By similarity
	Disulfide bond	36 ↔ 58		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7M135-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 2072A25C142A30A6	FASTA	269	27,961
	10 20 30 40 50 60 GVSGSCNIDV VCPEGNGHRD VIRSVAAYSK QGTMWCTGSL VNNSANDKKM YFLTANHCGM 70 80 90 100 110 120 TTAAIASSMV VYWNYQNSTC RAPGSSSSGA NGDGSLAQSQ TGAVVRATNA ASDFTLLELN 130 140 150 160 170 180 TAANPAYNLF WAGWDRRDQN FAGATAIHHP NVAEKRISHS TVATEISGYN GATGTSHLHV 190 200 210 220 230 240 FWQASGGVTE PGSSGSPIYS PEKRVLGQLH GGPSSCSATG ADRSDYYGRV FTSWTGGGTS 250 260 ATRLSDWLDA AGTGAQFIDG LDSTGTPPV

References

[1]	Mentele R., Eckerskorn C., Kellermann J., Strupat K., Hagmann J., Lottspeich F. Submitted (JUN-1997) to the PIR data bank Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	S77957.
3D structure databases
SMR	Q7M135. Positions 1-263.
ModBase	Search...
Protein family/group databases
MEROPS	S01.280.
Enzyme and pathway databases
BRENDA	3.4.21.50. 19052.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

LYSC_LYSEN

Accession

Primary (citable) accession number: Q7M135

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)