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Q7M0E3

- DEST_RAT

UniProt

Q7M0E3 - DEST_RAT

Protein

Destrin

Gene

Dstn

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.

    GO - Biological processi

    1. actin filament depolymerization Source: InterPro

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Destrin
    Alternative name(s):
    Actin-depolymerizing factor
    Short name:
    ADF
    Gene namesi
    Name:Dstn
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi1588366. Dstn.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cytoskeleton Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 165164DestrinPRO_0000214921Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine2 Publications
    Modified residuei3 – 31Phosphoserine1 Publication
    Modified residuei19 – 191N6-acetyllysineBy similarity

    Post-translational modificationi

    ISGylated.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ7M0E3.
    PRIDEiQ7M0E3.

    PTM databases

    PhosphoSiteiQ7M0E3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ7M0E3.

    Interactioni

    Protein-protein interaction databases

    IntActiQ7M0E3. 1 interaction.
    MINTiMINT-4568380.
    STRINGi10116.ENSRNOP00000007794.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7M0E3.
    SMRiQ7M0E3. Positions 2-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 153150ADF-HPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi30 – 345Nuclear localization signalSequence Analysis

    Sequence similaritiesi

    Belongs to the actin-binding proteins ADF family.Curated
    Contains 1 ADF-H domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG294392.
    GeneTreeiENSGT00440000033289.
    HOGENOMiHOG000039697.
    HOVERGENiHBG000381.
    InParanoidiQ7M0E3.
    KOiK10363.
    OMAiQMLPEKD.
    OrthoDBiEOG7353Z9.
    PhylomeDBiQ7M0E3.
    TreeFamiTF328601.

    Family and domain databases

    Gene3Di3.40.20.10. 1 hit.
    InterProiIPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR017904. ADF/Cofilin/Destrin.
    [Graphical view]
    PANTHERiPTHR11913. PTHR11913. 1 hit.
    PfamiPF00241. Cofilin_ADF. 1 hit.
    [Graphical view]
    PRINTSiPR00006. COFILIN.
    SMARTiSM00102. ADF. 1 hit.
    [Graphical view]
    PROSITEiPS51263. ADF_H. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q7M0E3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIVVE    50
    EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM 100
    FFLWAPEQAP LKSKMIYASS KDAIKKKFPG IKHEYQANGP EDLNRTSIAE 150
    KLGGSLIVAF EGSPV 165
    Length:165
    Mass (Da):18,534
    Last modified:February 20, 2007 - v3
    Checksum:iD395B8A5642A0D79
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141K → L AA sequence (PubMed:9877327)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CF111187 mRNA. No translation available.
    PIRiJE0223.
    RefSeqiNP_001028838.1. NM_001033666.1.
    UniGeneiRn.102412.

    Genome annotation databases

    EnsembliENSRNOT00000007794; ENSRNOP00000007794; ENSRNOG00000005924.
    GeneIDi502674.
    KEGGirno:502674.
    UCSCiRGD:1588366. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CF111187 mRNA. No translation available.
    PIRi JE0223.
    RefSeqi NP_001028838.1. NM_001033666.1.
    UniGenei Rn.102412.

    3D structure databases

    ProteinModelPortali Q7M0E3.
    SMRi Q7M0E3. Positions 2-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q7M0E3. 1 interaction.
    MINTi MINT-4568380.
    STRINGi 10116.ENSRNOP00000007794.

    PTM databases

    PhosphoSitei Q7M0E3.

    Proteomic databases

    PaxDbi Q7M0E3.
    PRIDEi Q7M0E3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007794 ; ENSRNOP00000007794 ; ENSRNOG00000005924 .
    GeneIDi 502674.
    KEGGi rno:502674.
    UCSCi RGD:1588366. rat.

    Organism-specific databases

    CTDi 11034.
    RGDi 1588366. Dstn.

    Phylogenomic databases

    eggNOGi NOG294392.
    GeneTreei ENSGT00440000033289.
    HOGENOMi HOG000039697.
    HOVERGENi HBG000381.
    InParanoidi Q7M0E3.
    KOi K10363.
    OMAi QMLPEKD.
    OrthoDBi EOG7353Z9.
    PhylomeDBi Q7M0E3.
    TreeFami TF328601.

    Miscellaneous databases

    NextBioi 712150.

    Gene expression databases

    Genevestigatori Q7M0E3.

    Family and domain databases

    Gene3Di 3.40.20.10. 1 hit.
    InterProi IPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR017904. ADF/Cofilin/Destrin.
    [Graphical view ]
    PANTHERi PTHR11913. PTHR11913. 1 hit.
    Pfami PF00241. Cofilin_ADF. 1 hit.
    [Graphical view ]
    PRINTSi PR00006. COFILIN.
    SMARTi SM00102. ADF. 1 hit.
    [Graphical view ]
    PROSITEi PS51263. ADF_H. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Lung.
    2. "Complete amino acid sequences and phosphorylation sites, determined by Edman degradation and mass spectrometry, of rat parotid destrin- and cofilin-like proteins."
      Kanamori T., Suzuki M., Titani K.
      Arch. Oral Biol. 43:955-967(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-165, ACETYLATION AT ALA-2, PHOSPHORYLATION AT SER-3, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Parotid gland.
    3. Lubec G., Afjehi-Sadat L., Diao W., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 46-69; 82-92 AND 133-145, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.
    4. Lubec G., Chen W.-Q.
      Submitted (FEB-2007) to UniProtKB
      Cited for: ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiDEST_RAT
    AccessioniPrimary (citable) accession number: Q7M0E3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 80 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3