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Q7LZG2

- PA2T_OXYSC

UniProt

Q7LZG2 - PA2T_OXYSC

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Protein

Phospholipase A2 taicatoxin

Gene
N/A
Organism
Oxyuranus scutellatus scutellatus (Australian taipan) (Coastal taipan)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Heterotrimer: blocks the voltage-dependent L-type calcium channels from the heart, and the small conductance calcium-activated potassium channels in the chromaffin cells and in the brain. Is very toxic to mice.
Monomer: Snake venom phospholipase A2 (PLA2) that has neurotoxic activities. Voltage-dependently affects ionic currents in chick (Gallus domesticus) dorsal root ganglion cells. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Binds 1 calcium ion.By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Calcium-activated potassium channel impairing toxin, Hydrolase, Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2 taicatoxin (EC:3.1.1.4)
Short name:
TCX
Short name:
svPLA2
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
OrganismiOxyuranus scutellatus scutellatus (Australian taipan) (Coastal taipan)
Taxonomic identifieri8667 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeAcanthophiinaeOxyuranus

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 of the monomer is 500 µg/kg to mice.
LD50 of the heterotrimer is 50-100 µg/kg to mice.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›27›27Phospholipase A2 taicatoxinPRO_0000408515Add
BLAST

Post-translational modificationi

Contains 7 disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.2 Publications

Interactioni

Subunit structurei

Heterotrimer composed of an alpha-neurotoxin-like peptide of 8 kDa (AC P0CJ35), this neurotoxic phospholipase of 16 kDa and a serine protease inhibitor of 7 kDa (AC B7S4N9) at an approximate stoichiometry of 1:1:4; non-covalently linked.1 Publication

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR016090. PLipase_A2_dom.
[Graphical view]
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.

Sequencei

Sequence statusi: Fragment.

Q7LZG2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20
NLAQFGFMIR CANGGSRSAL DYADYGC
Length:27
Mass (Da):2,901
Last modified:December 15, 2003 - v1
Checksum:iA74F18ACB843133C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei27 – 271

Sequence databases

PIRiA34280.
S21101.

Cross-referencesi

Web resourcesi

Wikipedia

Taicatoxin entry

Sequence databases

PIRi A34280.
S21101.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR016090. PLipase_A2_dom.
[Graphical view ]
Pfami PF00068. Phospholip_A2_1. 1 hit.
[Graphical view ]
SUPFAMi SSF48619. SSF48619. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Anionic currents of chick sensory neurons are affected by a phospholipase A2 purified from the venom of the taipan snake."
    Possani L.D., Mochca-Morales J., Amezcua J., Martin B.M., Prestipino G., Nobile M.
    Biochim. Biophys. Acta 1134:210-216(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LETHAL DOSE.
    Tissue: Venom.
  2. "Isolation and physiological characterization of taicatoxin, a complex toxin with specific effects on calcium channels."
    Possani L.D., Martin B.M., Yatani A., Mochca-Morales J., Zamudio F.Z., Gurrola G.B., Brown A.M.
    Toxicon 30:1343-1364(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LETHAL DOSE.
    Tissue: Venom.
  3. "Effect of TaiCatoxin (TCX) on the electrophysiological, mechanical and biochemical characteristics of spontaneously beating ventricular cardiomyocytes."
    Fantini E., Athias P., Tirosh R., Pinson A.
    Mol. Cell. Biochem. 160:61-66(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "A novel small conductance Ca2+-activated K+ channel blocker from Oxyuranus scutellatus taipan venom. Re-evaluation of taicatoxin as a selective Ca2+ channel probe."
    Doorty K.B., Bevan S., Wadsworth J.D.F., Strong P.N.
    J. Biol. Chem. 272:19925-19930(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Taicatoxin inhibits the calcium-dependent slow motility of mammalian outer hair cells."
    Su M.-C., Lee S.-Y., Tan C.-T., Su C.-C., Li S.-Y., Lin R.-H., Hung C.-C., Lin M.-J.
    Hear. Res. 203:172-179(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPA2T_OXYSC
AccessioniPrimary (citable) accession number: Q7LZG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: December 15, 2003
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3