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Q7LYT7

- Q7LYT7_PYRWO

UniProt

Q7LYT7 - Q7LYT7_PYRWO

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Protein
Submitted name:

Alpha amylase

Gene

amyA

Organism
Pyrococcus woesei
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491Magnesium 1; via carbonyl oxygen
Metal bindingi58 – 581Zinc 1Imported
Metal bindingi61 – 611Zinc 1Imported
Metal bindingi105 – 1051Magnesium 1
Metal bindingi105 – 1051Zinc 2Imported
Metal bindingi113 – 1131Magnesium 1
Metal bindingi113 – 1131Zinc 2Imported
Metal bindingi135 – 1351CalciumImported
Metal bindingi172 – 1721Zinc 3; via tele nitrogenImported
Metal bindingi177 – 1771Zinc 3; via tele nitrogenImported
Metal bindingi180 – 1801CalciumImported
Metal bindingi182 – 1821Calcium; via carbonyl oxygenImported
Metal bindingi189 – 1891CalciumImported
Metal bindingi191 – 1911Zinc 3Imported
Metal bindingi227 – 2271Calcium; via carbonyl oxygenImported
Metal bindingi277 – 2771Magnesium 2
Metal bindingi277 – 2771Zinc 4Imported
Metal bindingi281 – 2811Magnesium 2
Metal bindingi281 – 2811Zinc 4Imported
Metal bindingi317 – 3171Magnesium 2; via carbonyl oxygen
Metal bindingi317 – 3171Zinc 4Imported
Metal bindingi343 – 3431Zinc 1Imported
Binding sitei345 – 3451GlucoseImported
Metal bindingi348 – 3481Zinc 1Imported
Metal bindingi372 – 3721Magnesium 3
Metal bindingi372 – 3721Zinc 5Imported
Metal bindingi374 – 3741Magnesium 3
Metal bindingi374 – 3741Zinc 5Imported
Metal bindingi375 – 3751Magnesium 3
Metal bindingi375 – 3751Zinc 5Imported

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseImported, Hydrolase

Keywords - Ligandi

CalciumImported, MagnesiumImported, Metal-bindingImported, ZincImported

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha amylaseImported (EC:3.2.1.1Imported)
Gene namesi
Name:amyAImported
OrganismiPyrococcus woeseiImported
Taxonomic identifieri2262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MWOX-ray2.20A26-460[»]
1MXDX-ray2.00A26-460[»]
1MXGX-ray1.60A26-460[»]
ProteinModelPortaliQ7LYT7.
SMRiQ7LYT7. Positions 26-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni316 – 3216Glucose bindingImported

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015237. Alpha-amylase_C_pro.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF09154. DUF1939. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7LYT7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNIKKLTPLL TLLLFFIVLA SPVSAAKYLE LEEGGVIMQA FYWDVPGGGI
60 70 80 90 100
WWDHIRSKIP EWYEAGISAI WLPPPSKGMS GGYSMGYDPY DYFDLGEYYQ
110 120 130 140 150
KGTVETRFGS KEELVRLIQT AHAYGIKVIA DVVINHRAGG DLEWNPFVGD
160 170 180 190 200
YTWTDFSKVA SGKYTANYLD FHPNELHCCD EGTFGGFPDI CHHKEWDQYW
210 220 230 240 250
LWKSNESYAA YLRSIGFDGW RFDYVKGYGA WVVRDWLNWW GGWAVGEYWD
260 270 280 290 300
TNVDALLSWA YESGAKVFDF PLYYKMDEAF DNNNIPALVY ALQNGQTVVS
310 320 330 340 350
RDPFKAVTFV ANHDTDIIWN KYPAYAFILT YEGQPVIFYR DFEEWLNKDK
360 370 380 390 400
LINLIWIHDH LAGGSTTIVY YDNDELIFVR NGDSRRPGLI TYINLSPNWV
410 420 430 440 450
GRWVYVPKFA GACIHEYTGN LGGWVDKRVD SSGWVYLEAP PHDPANGYYG
460
YSVWSYCGVG
Length:460
Mass (Da):52,910
Last modified:July 5, 2004 - v1
Checksum:i0E0A2AEB5FCAF541
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF177906 Genomic DNA. Translation: AAD54338.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF177906 Genomic DNA. Translation: AAD54338.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MWO X-ray 2.20 A 26-460 [» ]
1MXD X-ray 2.00 A 26-460 [» ]
1MXG X-ray 1.60 A 26-460 [» ]
ProteinModelPortali Q7LYT7.
SMRi Q7LYT7. Positions 26-460.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR013776. A-amylase_thermo.
IPR015237. Alpha-amylase_C_pro.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF09154. DUF1939. 1 hit.
[Graphical view ]
PIRSFi PIRSF001021. Alph-amls_thrmst. 1 hit.
PRINTSi PR00110. ALPHAAMYLASE.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and Expression of Alpha Amylase from Pyrococcus woesei."
    Chunlin Lu, Weizheng J., Yunyan Y.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM3773Imported.
  2. "Differential regulation of a hyperthermophilic alpha-amylase with a novel (Ca,Zn) two-metal center by zinc."
    Linden A., Mayans O., Meyer-Klaucke W., Antranikian G., Wilmanns M.
    J. Biol. Chem. 278:9875-9884(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 26-460 IN COMPLEX WITH CALCIUM; GLUCOSE; MAGNESIUM AND ZINC.

Entry informationi

Entry nameiQ7LYT7_PYRWO
AccessioniPrimary (citable) accession number: Q7LYT7
Entry historyi
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3