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Q7LYT7 (Q7LYT7_PYRWO) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
EC=3.2.1.1 EMBL AAD54338.1
Gene names
Name:amyA EMBL AAD54338.1
OrganismPyrococcus woesei EMBL AAD54338.1
Taxonomic identifier2262 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region316 – 3216Glucose binding PDB 1MXD

Sites

Metal binding491Magnesium 1; via carbonyl oxygen PDB 1MXG
Metal binding581Zinc 1
Metal binding611Zinc 1
Metal binding1051Magnesium 1 PDB 1MXG
Metal binding1051Zinc 2
Metal binding1131Magnesium 1 PDB 1MXG
Metal binding1131Zinc 2
Metal binding1351Calcium PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding1721Zinc 3; via tele nitrogen PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding1771Zinc 3; via tele nitrogen PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding1801Calcium PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding1821Calcium; via carbonyl oxygen PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding1891Calcium PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding1911Zinc 3 PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding2271Calcium; via carbonyl oxygen PDB 1MWO PDB 1MXD PDB 1MXG
Metal binding2771Magnesium 2 PDB 1MXG
Metal binding2771Zinc 4
Metal binding2811Magnesium 2 PDB 1MXG
Metal binding2811Zinc 4
Metal binding3171Magnesium 2; via carbonyl oxygen PDB 1MXG
Metal binding3171Zinc 4
Metal binding3431Zinc 1
Metal binding3481Zinc 1
Metal binding3721Magnesium 3 PDB 1MXG
Metal binding3721Zinc 5
Metal binding3741Magnesium 3 PDB 1MXG
Metal binding3741Zinc 5
Metal binding3751Magnesium 3 PDB 1MXG
Metal binding3751Zinc 5
Binding site581Acetate PDB 1MXD
Binding site3431Acetate PDB 1MXD
Binding site3451Glucose PDB 1MXD

Sequences

Sequence LengthMass (Da)Tools
Q7LYT7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 0E0A2AEB5FCAF541

FASTA46052,910
        10         20         30         40         50         60 
MNIKKLTPLL TLLLFFIVLA SPVSAAKYLE LEEGGVIMQA FYWDVPGGGI WWDHIRSKIP 

        70         80         90        100        110        120 
EWYEAGISAI WLPPPSKGMS GGYSMGYDPY DYFDLGEYYQ KGTVETRFGS KEELVRLIQT 

       130        140        150        160        170        180 
AHAYGIKVIA DVVINHRAGG DLEWNPFVGD YTWTDFSKVA SGKYTANYLD FHPNELHCCD 

       190        200        210        220        230        240 
EGTFGGFPDI CHHKEWDQYW LWKSNESYAA YLRSIGFDGW RFDYVKGYGA WVVRDWLNWW 

       250        260        270        280        290        300 
GGWAVGEYWD TNVDALLSWA YESGAKVFDF PLYYKMDEAF DNNNIPALVY ALQNGQTVVS 

       310        320        330        340        350        360 
RDPFKAVTFV ANHDTDIIWN KYPAYAFILT YEGQPVIFYR DFEEWLNKDK LINLIWIHDH 

       370        380        390        400        410        420 
LAGGSTTIVY YDNDELIFVR NGDSRRPGLI TYINLSPNWV GRWVYVPKFA GACIHEYTGN 

       430        440        450        460 
LGGWVDKRVD SSGWVYLEAP PHDPANGYYG YSVWSYCGVG 

« Hide

References

[1]"Cloning and Expression of Alpha Amylase from Pyrococcus woesei."
Chunlin Lu, Weizheng J., Yunyan Y.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DSM3773 EMBL AAD54338.1.
[2]"Differential regulation of a hyperthermophilic alpha-amylase with a novel (Ca,Zn) two-metal center by zinc."
Linden A., Mayans O., Meyer-Klaucke W., Antranikian G., Wilmanns M.
J. Biol. Chem. 278:9875-9884(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 26-460 IN COMPLEX WITH ACETATE; CALCIUM; GLUCOSE; MAGNESIUM AND ZINC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF177906 Genomic DNA. Translation: AAD54338.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MWOX-ray2.20A26-460[»]
1MXDX-ray2.00A26-460[»]
1MXGX-ray1.60A26-460[»]
ProteinModelPortalQ7LYT7.
SMRQ7LYT7. Positions 26-460.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013776. A-amylase_thermo.
IPR015237. Alpha-amylase_C_pro.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF09154. DUF1939. 1 hit.
[Graphical view]
PIRSFPIRSF001021. Alph-amls_thrmst. 1 hit.
PRINTSPR00110. ALPHAAMYLASE.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ7LYT7_PYRWO
AccessionPrimary (citable) accession number: Q7LYT7
Entry history
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)