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Q7LYT7

- Q7LYT7_PYRWO

UniProt

Q7LYT7 - Q7LYT7_PYRWO

Protein
Submitted name:

Alpha amylase

Gene

amyA

Organism
Pyrococcus woesei
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi49 – 491Magnesium 1; via carbonyl oxygen
    Metal bindingi58 – 581Zinc 1Imported
    Metal bindingi61 – 611Zinc 1Imported
    Metal bindingi105 – 1051Magnesium 1
    Metal bindingi105 – 1051Zinc 2Imported
    Metal bindingi113 – 1131Magnesium 1
    Metal bindingi113 – 1131Zinc 2Imported
    Metal bindingi135 – 1351CalciumImported
    Metal bindingi172 – 1721Zinc 3; via tele nitrogenImported
    Metal bindingi177 – 1771Zinc 3; via tele nitrogenImported
    Metal bindingi180 – 1801CalciumImported
    Metal bindingi182 – 1821Calcium; via carbonyl oxygenImported
    Metal bindingi189 – 1891CalciumImported
    Metal bindingi191 – 1911Zinc 3Imported
    Metal bindingi227 – 2271Calcium; via carbonyl oxygenImported
    Metal bindingi277 – 2771Magnesium 2
    Metal bindingi277 – 2771Zinc 4Imported
    Metal bindingi281 – 2811Magnesium 2
    Metal bindingi281 – 2811Zinc 4Imported
    Metal bindingi317 – 3171Magnesium 2; via carbonyl oxygen
    Metal bindingi317 – 3171Zinc 4Imported
    Metal bindingi343 – 3431Zinc 1Imported
    Binding sitei345 – 3451GlucoseImported
    Metal bindingi348 – 3481Zinc 1Imported
    Metal bindingi372 – 3721Magnesium 3
    Metal bindingi372 – 3721Zinc 5Imported
    Metal bindingi374 – 3741Magnesium 3
    Metal bindingi374 – 3741Zinc 5Imported
    Metal bindingi375 – 3751Magnesium 3
    Metal bindingi375 – 3751Zinc 5Imported

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseImported, Hydrolase

    Keywords - Ligandi

    CalciumImported, MagnesiumImported, Metal-bindingImported, ZincImported

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Alpha amylaseImported (EC:3.2.1.1Imported)
    Gene namesi
    Name:amyAImported
    OrganismiPyrococcus woeseiImported
    Taxonomic identifieri2262 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MWOX-ray2.20A26-460[»]
    1MXDX-ray2.00A26-460[»]
    1MXGX-ray1.60A26-460[»]
    ProteinModelPortaliQ7LYT7.
    SMRiQ7LYT7. Positions 26-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni316 – 3216Glucose bindingImported

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013776. A-amylase_thermo.
    IPR015237. Alpha-amylase_C_pro.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF09154. DUF1939. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
    PRINTSiPR00110. ALPHAAMYLASE.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7LYT7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIKKLTPLL TLLLFFIVLA SPVSAAKYLE LEEGGVIMQA FYWDVPGGGI    50
    WWDHIRSKIP EWYEAGISAI WLPPPSKGMS GGYSMGYDPY DYFDLGEYYQ 100
    KGTVETRFGS KEELVRLIQT AHAYGIKVIA DVVINHRAGG DLEWNPFVGD 150
    YTWTDFSKVA SGKYTANYLD FHPNELHCCD EGTFGGFPDI CHHKEWDQYW 200
    LWKSNESYAA YLRSIGFDGW RFDYVKGYGA WVVRDWLNWW GGWAVGEYWD 250
    TNVDALLSWA YESGAKVFDF PLYYKMDEAF DNNNIPALVY ALQNGQTVVS 300
    RDPFKAVTFV ANHDTDIIWN KYPAYAFILT YEGQPVIFYR DFEEWLNKDK 350
    LINLIWIHDH LAGGSTTIVY YDNDELIFVR NGDSRRPGLI TYINLSPNWV 400
    GRWVYVPKFA GACIHEYTGN LGGWVDKRVD SSGWVYLEAP PHDPANGYYG 450
    YSVWSYCGVG 460
    Length:460
    Mass (Da):52,910
    Last modified:July 5, 2004 - v1
    Checksum:i0E0A2AEB5FCAF541
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177906 Genomic DNA. Translation: AAD54338.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177906 Genomic DNA. Translation: AAD54338.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MWO X-ray 2.20 A 26-460 [» ]
    1MXD X-ray 2.00 A 26-460 [» ]
    1MXG X-ray 1.60 A 26-460 [» ]
    ProteinModelPortali Q7LYT7.
    SMRi Q7LYT7. Positions 26-460.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013776. A-amylase_thermo.
    IPR015237. Alpha-amylase_C_pro.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF09154. DUF1939. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001021. Alph-amls_thrmst. 1 hit.
    PRINTSi PR00110. ALPHAAMYLASE.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and Expression of Alpha Amylase from Pyrococcus woesei."
      Chunlin Lu, Weizheng J., Yunyan Y.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM3773Imported.
    2. "Differential regulation of a hyperthermophilic alpha-amylase with a novel (Ca,Zn) two-metal center by zinc."
      Linden A., Mayans O., Meyer-Klaucke W., Antranikian G., Wilmanns M.
      J. Biol. Chem. 278:9875-9884(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 26-460 IN COMPLEX WITH CALCIUM; GLUCOSE; MAGNESIUM AND ZINC.

    Entry informationi

    Entry nameiQ7LYT7_PYRWO
    AccessioniPrimary (citable) accession number: Q7LYT7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3