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Protein

Probable tRNA pseudouridine synthase B

Gene

truB

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:PF1785
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Probable tRNA pseudouridine synthase BPRO_0000121968Add
BLAST

Proteomic databases

PRIDEiQ7LWY0.

Interactioni

Protein-protein interaction databases

DIPiDIP-48526N.
STRINGi186497.PF1785.

Structurei

Secondary structure

1
340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 133Combined sources
Beta strandi18 – 214Combined sources
Helixi35 – 373Combined sources
Helixi40 – 456Combined sources
Beta strandi47 – 537Combined sources
Beta strandi55 – 573Combined sources
Helixi59 – 6911Combined sources
Beta strandi75 – 795Combined sources
Beta strandi86 – 938Combined sources
Helixi94 – 996Combined sources
Helixi100 – 1034Combined sources
Beta strandi104 – 1063Combined sources
Beta strandi108 – 11811Combined sources
Helixi122 – 1309Combined sources
Beta strandi133 – 1386Combined sources
Beta strandi142 – 1443Combined sources
Beta strandi151 – 16414Combined sources
Beta strandi167 – 1748Combined sources
Helixi180 – 19011Combined sources
Beta strandi191 – 1933Combined sources
Beta strandi195 – 20511Combined sources
Beta strandi208 – 2114Combined sources
Beta strandi214 – 2163Combined sources
Helixi217 – 23014Combined sources
Helixi234 – 2385Combined sources
Beta strandi240 – 2423Combined sources
Helixi243 – 2475Combined sources
Beta strandi252 – 2554Combined sources
Helixi257 – 2637Combined sources
Turni264 – 2663Combined sources
Helixi271 – 2733Combined sources
Beta strandi274 – 2785Combined sources
Beta strandi286 – 2916Combined sources
Beta strandi296 – 3049Combined sources
Helixi306 – 3116Combined sources
Beta strandi314 – 32310Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EY4X-ray2.11A/B1-333[»]
2HVYX-ray2.30A1-340[»]
2RFKX-ray2.87A5-338[»]
3HAXX-ray2.11A1-340[»]
3HAYX-ray4.99A1-340[»]
3HJWX-ray2.35A8-334[»]
3HJYX-ray3.65A8-334[»]
3LWOX-ray2.86A1-340[»]
3LWPX-ray2.50A1-340[»]
3LWQX-ray2.68A1-340[»]
3LWRX-ray2.20A1-340[»]
3LWVX-ray2.50A1-340[»]
3MQKX-ray2.80A8-335[»]
ProteinModelPortaliQ7LWY0.
SMRiQ7LWY0. Positions 5-333.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7LWY0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini250 – 32576PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
HOGENOMiHOG000231224.
KOiK11131.
OMAiGPFKEDE.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7LWY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDEVRRIL PADIKREVLI KDENAETNPD WGFPPEKRPI EMHIQFGVIN
60 70 80 90 100
LDKPPGPTSH EVVAWIKKIL NLEKAGHGGT LDPKVSGVLP VALEKATRVV
110 120 130 140 150
QALLPAGKEY VALMHLHGDV PEDKIIQVMK EFEGEIIQRP PLRSAVKRRL
160 170 180 190 200
RTRKVYYIEV LEIEGRDVLF RVGVEAGTYI RSLIHHIGLA LGVGAHMSEL
210 220 230 240 250
RRTRSGPFKE DETLITLHDL VDYYYFWKED GIEEYFRKAI QPMEKAVEHL
260 270 280 290 300
PKVWIKDSAV AAVTHGADLA VPGIAKLHAG IKRGDLVAIM TLKDELVALG
310 320 330 340
KAMMTSQEML EKTKGIAVDV EKVFMPRDWY PKLWEKRDRS
Length:340
Mass (Da):38,561
Last modified:July 5, 2004 - v1
Checksum:i76CFE5095C5562FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81909.1.
RefSeqiWP_011012926.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81909; AAL81909; PF1785.
GeneIDi1469664.
KEGGipfu:PF1785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81909.1.
RefSeqiWP_011012926.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EY4X-ray2.11A/B1-333[»]
2HVYX-ray2.30A1-340[»]
2RFKX-ray2.87A5-338[»]
3HAXX-ray2.11A1-340[»]
3HAYX-ray4.99A1-340[»]
3HJWX-ray2.35A8-334[»]
3HJYX-ray3.65A8-334[»]
3LWOX-ray2.86A1-340[»]
3LWPX-ray2.50A1-340[»]
3LWQX-ray2.68A1-340[»]
3LWRX-ray2.20A1-340[»]
3LWVX-ray2.50A1-340[»]
3MQKX-ray2.80A8-335[»]
ProteinModelPortaliQ7LWY0.
SMRiQ7LWY0. Positions 5-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48526N.
STRINGi186497.PF1785.

Proteomic databases

PRIDEiQ7LWY0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81909; AAL81909; PF1785.
GeneIDi1469664.
KEGGipfu:PF1785.

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
HOGENOMiHOG000231224.
KOiK11131.
OMAiGPFKEDE.

Miscellaneous databases

EvolutionaryTraceiQ7LWY0.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRUB_PYRFU
AccessioniPrimary (citable) accession number: Q7LWY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.