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Q7LWY0 (TRUB_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable tRNA pseudouridine synthase B
EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:PF1785
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP MF_01081

Catalytic activity

tRNA uridine(55) = tRNA pseudouridine(55). HAMAP MF_01081

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processpseudouridine synthesis

Inferred from electronic annotation. Source: InterPro

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Probable tRNA pseudouridine synthase B HAMAP MF_01081
PRO_0000121968

Regions

Domain250 – 32576PUA

Sites

Active site821Nucleophile By similarity

Secondary structure

1 ........................................................... 340
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q7LWY0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 76CFE5095C5562FC

FASTA34038,561
        10         20         30         40         50         60 
MARDEVRRIL PADIKREVLI KDENAETNPD WGFPPEKRPI EMHIQFGVIN LDKPPGPTSH 

        70         80         90        100        110        120 
EVVAWIKKIL NLEKAGHGGT LDPKVSGVLP VALEKATRVV QALLPAGKEY VALMHLHGDV 

       130        140        150        160        170        180 
PEDKIIQVMK EFEGEIIQRP PLRSAVKRRL RTRKVYYIEV LEIEGRDVLF RVGVEAGTYI 

       190        200        210        220        230        240 
RSLIHHIGLA LGVGAHMSEL RRTRSGPFKE DETLITLHDL VDYYYFWKED GIEEYFRKAI 

       250        260        270        280        290        300 
QPMEKAVEHL PKVWIKDSAV AAVTHGADLA VPGIAKLHAG IKRGDLVAIM TLKDELVALG 

       310        320        330        340 
KAMMTSQEML EKTKGIAVDV EKVFMPRDWY PKLWEKRDRS

« Hide

References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE009950 Genomic DNA. Translation: AAL81909.1.
RefSeqNP_579514.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EY4X-ray2.11A/B1-333[»]
2HVYX-ray2.30A1-340[»]
2RFKX-ray2.87A5-338[»]
3HAXX-ray2.11A1-340[»]
3HAYX-ray4.99A1-340[»]
3HJWX-ray2.35A8-334[»]
3HJYX-ray3.65A8-334[»]
3LWOX-ray2.86A1-340[»]
3LWPX-ray2.50A1-340[»]
3LWQX-ray2.68A1-340[»]
3LWRX-ray2.20A1-340[»]
3LWVX-ray2.50A1-340[»]
3MQKX-ray2.80A8-335[»]
ProteinModelPortalQ7LWY0.
SMRQ7LWY0. Positions 5-333.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48526N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBPYRT00000004105; EBPYRP00000003966; EBPYRG00000004105.
GeneID1469664.
GenomeReviewsGene locus PF1785 in contig AE009950_GR.
KEGGpfu:PF1785.

Phylogenomic databases

GeneTreeEBGT00050000022953.
HOGENOMHBG397258.
OMAIYQRPPL.
PhylomeDBQ7LWY0.
ProtClustDBPRK04270.

Family and domain databases

HAMAPMF_01081. TruB_arch.
[Tree]
InterProIPR012960. Dyskerin-like.
IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR004802. Pseudouridine_synthase-related.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
KOK03177.
PANTHERPTHR23127. PTHR23127. 1 hit.
PfamPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR00425. CBF5. 1 hit.
TIGR00451. Unchar_dom_2. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRUB_PYRFU
AccessionPrimary (citable) accession number: Q7LWY0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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