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Protein

Probable tRNA pseudouridine synthase B

Gene

truB

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei82NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:PF1785
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219681 – 340Probable tRNA pseudouridine synthase BAdd BLAST340

Proteomic databases

PRIDEiQ7LWY0.

Interactioni

Protein-protein interaction databases

DIPiDIP-48526N.
STRINGi186497.PF1785.

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 13Combined sources3
Beta strandi18 – 21Combined sources4
Helixi35 – 37Combined sources3
Helixi40 – 45Combined sources6
Beta strandi47 – 53Combined sources7
Beta strandi55 – 57Combined sources3
Helixi59 – 69Combined sources11
Beta strandi75 – 79Combined sources5
Beta strandi86 – 93Combined sources8
Helixi94 – 99Combined sources6
Helixi100 – 103Combined sources4
Beta strandi104 – 106Combined sources3
Beta strandi108 – 118Combined sources11
Helixi122 – 130Combined sources9
Beta strandi133 – 138Combined sources6
Beta strandi142 – 144Combined sources3
Beta strandi151 – 164Combined sources14
Beta strandi167 – 174Combined sources8
Helixi180 – 190Combined sources11
Beta strandi191 – 193Combined sources3
Beta strandi195 – 205Combined sources11
Beta strandi208 – 211Combined sources4
Beta strandi214 – 216Combined sources3
Helixi217 – 230Combined sources14
Helixi234 – 238Combined sources5
Beta strandi240 – 242Combined sources3
Helixi243 – 247Combined sources5
Beta strandi252 – 255Combined sources4
Helixi257 – 263Combined sources7
Turni264 – 266Combined sources3
Helixi271 – 273Combined sources3
Beta strandi274 – 278Combined sources5
Beta strandi286 – 291Combined sources6
Beta strandi296 – 304Combined sources9
Helixi306 – 311Combined sources6
Beta strandi314 – 323Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EY4X-ray2.11A/B1-333[»]
2HVYX-ray2.30A1-340[»]
2RFKX-ray2.87A5-338[»]
3HAXX-ray2.11A1-340[»]
3HAYX-ray4.99A1-340[»]
3HJWX-ray2.35A8-334[»]
3HJYX-ray3.65A8-334[»]
3LWOX-ray2.86A1-340[»]
3LWPX-ray2.50A1-340[»]
3LWQX-ray2.68A1-340[»]
3LWRX-ray2.20A1-340[»]
3LWVX-ray2.50A1-340[»]
3MQKX-ray2.80A8-335[»]
ProteinModelPortaliQ7LWY0.
SMRiQ7LWY0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7LWY0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini250 – 325PUAUniRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
HOGENOMiHOG000231224.
KOiK11131.
OMAiGPFKEDE.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7LWY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDEVRRIL PADIKREVLI KDENAETNPD WGFPPEKRPI EMHIQFGVIN
60 70 80 90 100
LDKPPGPTSH EVVAWIKKIL NLEKAGHGGT LDPKVSGVLP VALEKATRVV
110 120 130 140 150
QALLPAGKEY VALMHLHGDV PEDKIIQVMK EFEGEIIQRP PLRSAVKRRL
160 170 180 190 200
RTRKVYYIEV LEIEGRDVLF RVGVEAGTYI RSLIHHIGLA LGVGAHMSEL
210 220 230 240 250
RRTRSGPFKE DETLITLHDL VDYYYFWKED GIEEYFRKAI QPMEKAVEHL
260 270 280 290 300
PKVWIKDSAV AAVTHGADLA VPGIAKLHAG IKRGDLVAIM TLKDELVALG
310 320 330 340
KAMMTSQEML EKTKGIAVDV EKVFMPRDWY PKLWEKRDRS
Length:340
Mass (Da):38,561
Last modified:July 5, 2004 - v1
Checksum:i76CFE5095C5562FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81909.1.
RefSeqiWP_011012926.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81909; AAL81909; PF1785.
GeneIDi1469664.
KEGGipfu:PF1785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81909.1.
RefSeqiWP_011012926.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EY4X-ray2.11A/B1-333[»]
2HVYX-ray2.30A1-340[»]
2RFKX-ray2.87A5-338[»]
3HAXX-ray2.11A1-340[»]
3HAYX-ray4.99A1-340[»]
3HJWX-ray2.35A8-334[»]
3HJYX-ray3.65A8-334[»]
3LWOX-ray2.86A1-340[»]
3LWPX-ray2.50A1-340[»]
3LWQX-ray2.68A1-340[»]
3LWRX-ray2.20A1-340[»]
3LWVX-ray2.50A1-340[»]
3MQKX-ray2.80A8-335[»]
ProteinModelPortaliQ7LWY0.
SMRiQ7LWY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48526N.
STRINGi186497.PF1785.

Proteomic databases

PRIDEiQ7LWY0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81909; AAL81909; PF1785.
GeneIDi1469664.
KEGGipfu:PF1785.

Phylogenomic databases

eggNOGiarCOG00987. Archaea.
COG0130. LUCA.
HOGENOMiHOG000231224.
KOiK11131.
OMAiGPFKEDE.

Miscellaneous databases

EvolutionaryTraceiQ7LWY0.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01081. TruB_arch. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR032819. TruB_C.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRUB_PYRFU
AccessioniPrimary (citable) accession number: Q7LWY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.