Q7LGA3 (HS2ST_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heparan sulfate 2-O-sulfotransferase 1
Short name=2-O-sulfotransferase
Short name=2OST
EC=2.8.2.-

Gene names

Name:	HS2ST1
Synonyms:	HS2ST, KIAA0448

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinity ligand-binding interactions and signaling processes. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues By similarity.
Subunit structure	Homotrimer. Interacts with the C5-epimerase GLCE By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the sulfotransferase 3 family.
Sequence caution	The sequence BAA32293.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome glycosaminoglycan biosynthetic process Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sulfotransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7LGA3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7LGA3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRSKL → MLFIKFIHLEVFSMP
     282-356: GKKSHLRKTT...FYEKIYPKSN → APDTATPSHR...KCSRNGLSAV

Note: No experimental confirmation available.

Isoform 3 (identifier: Q7LGA3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     230-356: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Heparan sulfate 2-O-sulfotransferase 1		PRO_0000207674
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 28	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 356	328	Lumenal Potential
Sites
	Active site	140	1	By similarity
Amino acid modifications
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Disulfide bond	201 ↔ 209		By similarity
	Disulfide bond	222 ↔ 228		By similarity
Natural variations
	Alternative sequence	1 – 41	41	MGLLR…SRSKL → MLFIKFIHLEVFSMP in isoform 2.		VSP_014062
	Alternative sequence	230 – 356	127	Missing in isoform 3.		VSP_014063
	Alternative sequence	282 – 356	75	GKKSH…YPKSN → APDTATPSHRHGPICGSKSI SSLLVKVSPVCKDSHCLGKC SRNGLSAV in isoform 2.		VSP_014064

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2F5BBDFC7DF50F78	FASTA	356	41,881
	10 20 30 40 50 60 MGLLRIMMPP KLQLLAVVAF AVAMLFLENQ IQKLEESRSK LERAIARHEV REIEQRHTMD 70 80 90 100 110 120 GPRQDATLDE EEDMVIIYNR VPKTASTSFT NIAYDLCAKN KYHVLHINTT KNNPVMSLQD 130 140 150 160 170 180 QVRFVKNITS WKEMKPGFYH GHVSYLDFAK FGVKKKPIYI NVIRDPIERL VSYYYFLRFG 190 200 210 220 230 240 DDYRPGLRRR KQGDKKTFDE CVAEGGSDCA PEKLWLQIPF FCGHSSECWN VGSRWAMDQA 250 260 270 280 290 300 KYNLINEYFL VGVTEELEDF IMLLEAALPR FFRGATELYR TGKKSHLRKT TEKKLPTKQT 310 320 330 340 350 IAKLQQSDIW KMENEFYEFA LEQFQFIRAH AVREKDGDLY ILAQNFFYEK IYPKSN
	Isoform 2 [UniParc]. Checksum: 33F937D451024A45	FASTA	303	34,907
	10 20 30 40 50 60 MLFIKFIHLE VFSMPERAIA RHEVREIEQR HTMDGPRQDA TLDEEEDMVI IYNRVPKTAS 70 80 90 100 110 120 TSFTNIAYDL CAKNKYHVLH INTTKNNPVM SLQDQVRFVK NITSWKEMKP GFYHGHVSYL 130 140 150 160 170 180 DFAKFGVKKK PIYINVIRDP IERLVSYYYF LRFGDDYRPG LRRRKQGDKK TFDECVAEGG 190 200 210 220 230 240 SDCAPEKLWL QIPFFCGHSS ECWNVGSRWA MDQAKYNLIN EYFLVGVTEE LEDFIMLLEA 250 260 270 280 290 300 ALPRFFRGAT ELYRTAPDTA TPSHRHGPIC GSKSISSLLV KVSPVCKDSH CLGKCSRNGL SAV
	Isoform 3 [UniParc]. Checksum: F953C9470254BE9D	FASTA	229	26,757
	10 20 30 40 50 60 MGLLRIMMPP KLQLLAVVAF AVAMLFLENQ IQKLEESRSK LERAIARHEV REIEQRHTMD 70 80 90 100 110 120 GPRQDATLDE EEDMVIIYNR VPKTASTSFT NIAYDLCAKN KYHVLHINTT KNNPVMSLQD 130 140 150 160 170 180 QVRFVKNITS WKEMKPGFYH GHVSYLDFAK FGVKKKPIYI NVIRDPIERL VSYYYFLRFG 190 200 210 220 DDYRPGLRRR KQGDKKTFDE CVAEGGSDCA PEKLWLQIPF FCGHSSECW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human heparan sulfate 2-O-sulfotransferase." Habuchi H. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain." Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O. DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain, Ovary and Testis.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB024568 mRNA. Translation: BAA89250.1. AB007917 mRNA. Translation: BAA32293.2. Different initiation. AK002179 mRNA. Translation: BAA92125.1. AL139139, AC093155 Genomic DNA. Translation: CAI19004.1. AL121989 Genomic DNA. Translation: CAC04187.1. CH471097 Genomic DNA. Translation: EAW73171.1. CH471097 Genomic DNA. Translation: EAW73172.1. BC025384 mRNA. Translation: AAH25384.1. BC025990 mRNA. Translation: AAH25990.1. BC108735 mRNA. Translation: AAI08736.1.
IPI	IPI00040900. IPI00549891. IPI00552241.
RefSeq	NP_001127964.1. NM_001134492.1. NP_036394.1. NM_012262.3.
UniGene	Hs.48823.
3D structure databases
ProteinModelPortal	Q7LGA3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000359581.
PTM databases
PhosphoSite	Q7LGA3.
Polymorphism databases
DMDM	68052326.
Proteomic databases
PaxDb	Q7LGA3.
PRIDE	Q7LGA3.
Protocols and materials databases
DNASU	9653.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370550; ENSP00000359581; ENSG00000153936. ENST00000370551; ENSP00000359582; ENSG00000153936.
GeneID	9653.
KEGG	hsa:9653.
UCSC	uc001dmc.4. human. uc001dme.2. human.
Organism-specific databases
CTD	9653.
GeneCards	GC01P087380.
H-InvDB	HIX0000755.
HGNC	HGNC:5193. HS2ST1.
HPA	HPA043069. HPA043603.
MIM	604844. gene.
neXtProt	NX_Q7LGA3.
PharmGKB	PA29466.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG293591.
HOVERGEN	HBG053202.
InParanoid	Q7LGA3.
KO	K02513.
OMA	HAAECWE.
OrthoDB	EOG4VT5XH.
PhylomeDB	Q7LGA3.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	Q7LGA3.
Bgee	Q7LGA3.
CleanEx	HS_HS2ST1.
Genevestigator	Q7LGA3.
GermOnline	ENSG00000153936. Homo sapiens.
Family and domain databases
InterPro	IPR007734. Heparan_SO4_2-O-STrfase. IPR005331. Sulfotransferase. [Graphical view]
PANTHER	PTHR12129. PTHR12129. 1 hit.
Pfam	PF03567. Sulfotransfer_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9653.
NextBio	36237.
SOURCE	Search...

Entry information

Entry name

HS2ST_HUMAN

Accession

Primary (citable) accession number: Q7LGA3
Secondary accession number(s): D3DT22 , O75036, Q32NB5, Q8TAC5, Q9H441, Q9NUJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.