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Protein

Charged multivesicular body protein 1b

Gene

CHMP1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release.2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB
  • cell separation after cytokinesis Source: UniProtKB
  • ESCRT III complex disassembly Source: ParkinsonsUK-UCL
  • establishment of protein localization Source: UniProtKB
  • mitotic metaphase plate congression Source: UniProtKB
  • multivesicular body assembly Source: ParkinsonsUK-UCL
  • nucleus organization Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • regulation of centrosome duplication Source: UniProtKB
  • regulation of mitotic spindle assembly Source: UniProtKB
  • vacuolar transport Source: InterPro
  • viral budding via host ESCRT complex Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 1b
Alternative name(s):
CHMP1.5
Chromatin-modifying protein 1b
Short name:
CHMP1b
Vacuolar protein sorting-associated protein 46-2
Short name:
Vps46-2
Short name:
hVps46-2
Gene namesi
Name:CHMP1B
Synonyms:C18orf2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:24287. CHMP1B.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • endosome membrane Source: AgBase
  • ESCRT III complex Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • late endosome membrane Source: UniProtKB-SubCell
  • membrane coat Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi158 – 159DE → AA: Diminishes interaction with VPS4B. 1 Publication2
Mutagenesisi178T → R: Abolishes interaction with SPAST and no effect on interaction with VPS4A; when associated with R-181 and R-184. 1 Publication1
Mutagenesisi181A → R: Abolishes interaction with SPAScT and no effect on interaction with VPS4A; when associated with R-178 and R-184. 1 Publication1
Mutagenesisi184E → A: Decreases interaction with SPAST. 1 Publication1
Mutagenesisi184E → R: Abolishes interaction with SPAST and no effect on interaction with VPS4A; when associated with R-178 and R-181. 1 Publication1
Mutagenesisi188L → A: Abolishes interaction with SPAST and VPS4A; when associated with A-192. 1 Publication1
Mutagenesisi192L → A: Abolishes interaction with SPAST and VPS4A; when associated with A-188. 2 Publications1
Mutagenesisi192L → A: Abolishes interaction with VPS4B. 2 Publications1
Mutagenesisi195L → A: Abolishes interaction with VPS4B. 1 Publication1

Organism-specific databases

DisGeNETi57132.
OpenTargetsiENSG00000255112.
PharmGKBiPA142672110.

Polymorphism and mutation databases

BioMutaiCHMP1B.
DMDMi73917735.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114541 – 199Charged multivesicular body protein 1bAdd BLAST199

Proteomic databases

EPDiQ7LBR1.
MaxQBiQ7LBR1.
PaxDbiQ7LBR1.
PeptideAtlasiQ7LBR1.
PRIDEiQ7LBR1.

PTM databases

iPTMnetiQ7LBR1.
PhosphoSitePlusiQ7LBR1.

Expressioni

Tissue specificityi

Widely expressed. Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta and brain.1 Publication

Gene expression databases

BgeeiENSG00000255112.
CleanExiHS_C18orf2.
HS_CHMP1B.
GenevisibleiQ7LBR1. HS.

Organism-specific databases

HPAiHPA061997.
HPA062896.

Interactioni

Subunit structurei

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with CHMP1A. Interacts with VTA1; the interaction probably involves the open conformation of CHMP1B. Interacts with CHMP2A. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with SPAST (via MIT domain); the interaction is direct. Interacts with IST1. Interacts with MITD1. Interacts with STAMBP.11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAB11AP624913EBI-2118090,EBI-745098
SNRNP200O756433EBI-2118090,EBI-1045395
STAMBPO9563015EBI-2118090,EBI-396676
USP8P408185EBI-2118090,EBI-1050865
VPS4AQ9UN373EBI-2118090,EBI-1171942

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi121394. 32 interactors.
DIPiDIP-48534N.
IntActiQ7LBR1. 32 interactors.
MINTiMINT-6946813.
STRINGi9606.ENSP00000432279.

Structurei

Secondary structure

1199
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi151 – 159Combined sources9
Helixi187 – 196Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EABX-ray2.50G/H/I/J/K/L148-197[»]
3JC1electron microscopy4.00Ab/Ad/Af/Ah/Aj/Al/An/Ap/Ar/At/Av/Ax/Az/Bb/Bd/Bf/Bh/Bj/Bl/Bn/Bp/Br/Bt/Bv/Bx/Bz/Cb/Cd/Cf/Ch4-163[»]
4TXQX-ray2.21C/D176-199[»]
4TXRX-ray1.00B176-199[»]
ProteinModelPortaliQ7LBR1.
SMRiQ7LBR1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7LBR1.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 156Interaction with IST1Add BLAST25
Regioni174 – 199Interaction with SPAST1 PublicationAdd BLAST26
Regioni180 – 199Interaction with VTA1Add BLAST20
Regioni180 – 196Interaction with VPS4A, MITD1 and STAMBP1 PublicationAdd BLAST17
Regioni183 – 199Interaction with VPS4BAdd BLAST17

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili26 – 48Sequence analysisAdd BLAST23
Coiled coili178 – 199Sequence analysisAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi186 – 196MIT-interacting motifAdd BLAST11

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3232. Eukaryota.
COG5491. LUCA.
GeneTreeiENSGT00550000074957.
HOVERGENiHBG080200.
InParanoidiQ7LBR1.
KOiK12197.
OMAiTRKVTQS.
OrthoDBiEOG091G0S04.
PhylomeDBiQ7LBR1.
TreeFamiTF300076.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7LBR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNMEKHLFN LKFAAKELSR SAKKCDKEEK AEKAKIKKAI QKGNMEVARI
60 70 80 90 100
HAENAIRQKN QAVNFLRMSA RVDAVAARVQ TAVTMGKVTK SMAGVVKSMD
110 120 130 140 150
ATLKTMNLEK ISALMDKFEH QFETLDVQTQ QMEDTMSSTT TLTTPQNQVD
160 170 180 190
MLLQEMADEA GLDLNMELPQ GQTGSVGTSV ASAEQDELSQ RLARLRDQV
Length:199
Mass (Da):22,109
Last modified:July 5, 2004 - v1
Checksum:i8071E94D20D85AB5
GO

Sequence cautioni

The sequence AAG01449 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF306520 Genomic DNA. Translation: AAL48200.1.
AF281064 mRNA. Translation: AAG01449.1. Different initiation.
BC065933 mRNA. Translation: AAH65933.1.
BC012733 mRNA. Translation: AAH12733.3.
CCDSiCCDS54180.1.
RefSeqiNP_065145.2. NM_020412.4.
UniGeneiHs.656244.

Genome annotation databases

EnsembliENST00000526991; ENSP00000432279; ENSG00000255112.
GeneIDi57132.
KEGGihsa:57132.
UCSCiuc002kqe.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF306520 Genomic DNA. Translation: AAL48200.1.
AF281064 mRNA. Translation: AAG01449.1. Different initiation.
BC065933 mRNA. Translation: AAH65933.1.
BC012733 mRNA. Translation: AAH12733.3.
CCDSiCCDS54180.1.
RefSeqiNP_065145.2. NM_020412.4.
UniGeneiHs.656244.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EABX-ray2.50G/H/I/J/K/L148-197[»]
3JC1electron microscopy4.00Ab/Ad/Af/Ah/Aj/Al/An/Ap/Ar/At/Av/Ax/Az/Bb/Bd/Bf/Bh/Bj/Bl/Bn/Bp/Br/Bt/Bv/Bx/Bz/Cb/Cd/Cf/Ch4-163[»]
4TXQX-ray2.21C/D176-199[»]
4TXRX-ray1.00B176-199[»]
ProteinModelPortaliQ7LBR1.
SMRiQ7LBR1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121394. 32 interactors.
DIPiDIP-48534N.
IntActiQ7LBR1. 32 interactors.
MINTiMINT-6946813.
STRINGi9606.ENSP00000432279.

PTM databases

iPTMnetiQ7LBR1.
PhosphoSitePlusiQ7LBR1.

Polymorphism and mutation databases

BioMutaiCHMP1B.
DMDMi73917735.

Proteomic databases

EPDiQ7LBR1.
MaxQBiQ7LBR1.
PaxDbiQ7LBR1.
PeptideAtlasiQ7LBR1.
PRIDEiQ7LBR1.

Protocols and materials databases

DNASUi57132.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000526991; ENSP00000432279; ENSG00000255112.
GeneIDi57132.
KEGGihsa:57132.
UCSCiuc002kqe.4. human.

Organism-specific databases

CTDi57132.
DisGeNETi57132.
GeneCardsiCHMP1B.
HGNCiHGNC:24287. CHMP1B.
HPAiHPA061997.
HPA062896.
MIMi606486. gene.
neXtProtiNX_Q7LBR1.
OpenTargetsiENSG00000255112.
PharmGKBiPA142672110.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3232. Eukaryota.
COG5491. LUCA.
GeneTreeiENSGT00550000074957.
HOVERGENiHBG080200.
InParanoidiQ7LBR1.
KOiK12197.
OMAiTRKVTQS.
OrthoDBiEOG091G0S04.
PhylomeDBiQ7LBR1.
TreeFamiTF300076.

Miscellaneous databases

EvolutionaryTraceiQ7LBR1.
GeneWikiiCHMP1B.
GenomeRNAii57132.
PROiQ7LBR1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000255112.
CleanExiHS_C18orf2.
HS_CHMP1B.
GenevisibleiQ7LBR1. HS.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHM1B_HUMAN
AccessioniPrimary (citable) accession number: Q7LBR1
Secondary accession number(s): Q96E89, Q9HD41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.