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Q7LBR1

- CHM1B_HUMAN

UniProt

Q7LBR1 - CHM1B_HUMAN

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Protein

Charged multivesicular body protein 1b

Gene

CHMP1B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release.2 Publications

GO - Molecular functioni

  1. protein domain specific binding Source: UniProtKB

GO - Biological processi

  1. cytokinesis Source: UniProtKB
  2. establishment of protein localization Source: UniProt
  3. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 1b
Alternative name(s):
CHMP1.5
Chromatin-modifying protein 1b
Short name:
CHMP1b
Vacuolar protein sorting-associated protein 46-2
Short name:
Vps46-2
Short name:
hVps46-2
Gene namesi
Name:CHMP1B
Synonyms:C18orf2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:24287. CHMP1B.

Subcellular locationi

Cytoplasmcytosol. Endosome. Late endosome membrane Curated; Peripheral membrane protein Curated
Note: Localizes to the midbody of dividing cells, colocalizing with CEP55 and CHMP5. Localized at the periphery of the Fleming body.

GO - Cellular componenti

  1. endosome Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: UniProtKB
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi158 – 1592DE → AA: Diminishes interaction with VPS4B. 1 Publication
Mutagenesisi178 – 1781T → R: Abolishes interaction with SPAST and no effect on interaction with VPS4A; when associated with R-181 and R-184. 1 Publication
Mutagenesisi181 – 1811A → R: Abolishes interaction with SPAScT and no effect on interaction with VPS4A; when associated with R-178 and R-184. 1 Publication
Mutagenesisi184 – 1841E → A: Decreases interaction with SPAST. 1 Publication
Mutagenesisi184 – 1841E → R: Abolishes interaction with SPAST and no effect on interaction with VPS4A; when associated with R-178 and R-181. 1 Publication
Mutagenesisi188 – 1881L → A: Abolishes interaction with SPAST and VPS4A; when associated with A-192. 1 Publication
Mutagenesisi192 – 1921L → A: Abolishes interaction with SPAST and VPS4A; when associated with A-188. 2 Publications
Mutagenesisi192 – 1921L → A: Abolishes interaction with VPS4B. 2 Publications
Mutagenesisi195 – 1951L → A: Abolishes interaction with VPS4B. 1 Publication

Organism-specific databases

PharmGKBiPA142672110.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Charged multivesicular body protein 1bPRO_0000211454Add
BLAST

Proteomic databases

MaxQBiQ7LBR1.
PeptideAtlasiQ7LBR1.
PRIDEiQ7LBR1.

PTM databases

PhosphoSiteiQ7LBR1.

Expressioni

Tissue specificityi

Widely expressed. Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta and brain.1 Publication

Gene expression databases

BgeeiQ7LBR1.
CleanExiHS_C18orf2.
HS_CHMP1B.
ExpressionAtlasiQ7LBR1. baseline and differential.
GenevestigatoriQ7LBR1.

Interactioni

Subunit structurei

Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with CHMP1A. Interacts with VTA1; the interaction probably involves the open conformation of CHMP1B. Interacts with CHMP2A. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with SPAST (via MIT domain); the interaction is direct. Interacts with IST1. Interacts with MITD1. Interacts with STAMBP.11 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
STAMBPO956305EBI-2118090,EBI-396676
USP8P408185EBI-2118090,EBI-1050865

Protein-protein interaction databases

BioGridi121394. 20 interactions.
DIPiDIP-48534N.
IntActiQ7LBR1. 9 interactions.
MINTiMINT-6946813.

Structurei

Secondary structure

1
199
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi151 – 1599
Helixi174 – 19320

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3EABX-ray2.50G/H/I/J/K/L148-197[»]
ProteinModelPortaliQ7LBR1.
SMRiQ7LBR1. Positions 148-197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7LBR1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 15625Interaction with IST1Add
BLAST
Regioni174 – 19926Interaction with SPASTAdd
BLAST
Regioni180 – 19920Interaction with VTA1Add
BLAST
Regioni180 – 19617Interaction with VPS4A, MITD1 and STAMBPAdd
BLAST
Regioni183 – 19917Interaction with VPS4BAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 4823Sequence AnalysisAdd
BLAST
Coiled coili178 – 19922Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi186 – 19611MIT-interacting motifAdd
BLAST

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074957.
HOVERGENiHBG080200.
InParanoidiQ7LBR1.
KOiK12197.
OMAiLWRNIEN.
OrthoDBiEOG7BP843.
PhylomeDBiQ7LBR1.
TreeFamiTF300076.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7LBR1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNMEKHLFN LKFAAKELSR SAKKCDKEEK AEKAKIKKAI QKGNMEVARI
60 70 80 90 100
HAENAIRQKN QAVNFLRMSA RVDAVAARVQ TAVTMGKVTK SMAGVVKSMD
110 120 130 140 150
ATLKTMNLEK ISALMDKFEH QFETLDVQTQ QMEDTMSSTT TLTTPQNQVD
160 170 180 190
MLLQEMADEA GLDLNMELPQ GQTGSVGTSV ASAEQDELSQ RLARLRDQV
Length:199
Mass (Da):22,109
Last modified:July 5, 2004 - v1
Checksum:i8071E94D20D85AB5
GO

Sequence cautioni

The sequence AAG01449.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF306520 Genomic DNA. Translation: AAL48200.1.
AF281064 mRNA. Translation: AAG01449.1. Different initiation.
BC065933 mRNA. Translation: AAH65933.1.
BC012733 mRNA. Translation: AAH12733.3.
CCDSiCCDS54180.1.
RefSeqiNP_065145.2. NM_020412.4.
UniGeneiHs.656244.

Genome annotation databases

EnsembliENST00000526991; ENSP00000432279; ENSG00000255112.
GeneIDi57132.
KEGGihsa:57132.
UCSCiuc002kqe.3. human.

Polymorphism databases

DMDMi73917735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF306520 Genomic DNA. Translation: AAL48200.1 .
AF281064 mRNA. Translation: AAG01449.1 . Different initiation.
BC065933 mRNA. Translation: AAH65933.1 .
BC012733 mRNA. Translation: AAH12733.3 .
CCDSi CCDS54180.1.
RefSeqi NP_065145.2. NM_020412.4.
UniGenei Hs.656244.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3EAB X-ray 2.50 G/H/I/J/K/L 148-197 [» ]
ProteinModelPortali Q7LBR1.
SMRi Q7LBR1. Positions 148-197.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121394. 20 interactions.
DIPi DIP-48534N.
IntActi Q7LBR1. 9 interactions.
MINTi MINT-6946813.

PTM databases

PhosphoSitei Q7LBR1.

Polymorphism databases

DMDMi 73917735.

Proteomic databases

MaxQBi Q7LBR1.
PeptideAtlasi Q7LBR1.
PRIDEi Q7LBR1.

Protocols and materials databases

DNASUi 57132.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000526991 ; ENSP00000432279 ; ENSG00000255112 .
GeneIDi 57132.
KEGGi hsa:57132.
UCSCi uc002kqe.3. human.

Organism-specific databases

CTDi 57132.
GeneCardsi GC18P011851.
HGNCi HGNC:24287. CHMP1B.
MIMi 606486. gene.
neXtProti NX_Q7LBR1.
PharmGKBi PA142672110.
GenAtlasi Search...

Phylogenomic databases

GeneTreei ENSGT00550000074957.
HOVERGENi HBG080200.
InParanoidi Q7LBR1.
KOi K12197.
OMAi LWRNIEN.
OrthoDBi EOG7BP843.
PhylomeDBi Q7LBR1.
TreeFami TF300076.

Miscellaneous databases

EvolutionaryTracei Q7LBR1.
GeneWikii CHMP1B.
GenomeRNAii 57132.
NextBioi 63047.
PROi Q7LBR1.
SOURCEi Search...

Gene expression databases

Bgeei Q7LBR1.
CleanExi HS_C18orf2.
HS_CHMP1B.
ExpressionAtlasi Q7LBR1. baseline and differential.
Genevestigatori Q7LBR1.

Family and domain databases

InterProi IPR005024. Snf7_fam.
[Graphical view ]
Pfami PF03357. Snf7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "C18orf2, a novel, highly conserved intronless gene within intron 5 of the GNAL gene on chromosome 18p11."
    Vuoristo J.T., Berrettini W.H., Ala-Kokko L.
    Cytogenet. Cell Genet. 93:19-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  2. "CHMP1 is a novel nuclear matrix protein affecting chromatin structure and cell-cycle progression."
    Stauffer D.R., Howard T.L., Nyun T., Hollenberg S.M.
    J. Cell Sci. 114:2383-2393(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Melanoma and Ovary.
  4. Cited for: INTERACTION WITH CHMP1A; VPS4A AND VPS4B.
  5. "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins."
    Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
    Proc. Natl. Acad. Sci. U.S.A. 100:12414-12419(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HIV-1 BUDDING, INTERACTION WITH CHMP1A; CHMP2A AND VPS4A.
  6. Erratum
    Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
    Proc. Natl. Acad. Sci. U.S.A. 100:152845-152845(2003)
  7. "The hereditary spastic paraplegia protein spastin interacts with the ESCRT-III complex-associated endosomal protein CHMP1B."
    Reid E., Connell J.W., Edwards T.L., Duley S., Brown S.E., Sanderson C.M.
    Hum. Mol. Genet. 14:19-38(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SPAST.
  8. Cited for: INTERACTION WITH VPS4A.
  9. "A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex."
    Tsang H.T.H., Connell J.W., Brown S.E., Thompson A., Reid E., Sanderson C.M.
    Genomics 88:333-346(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH STAMBP.
  10. Cited for: INTERACTION WITH VPS4A AND VPS4B, MUTAGENESIS OF LEU-192 AND LEU-195.
  11. "Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly."
    Shim S., Merrill S.A., Hanson P.I.
    Mol. Biol. Cell 19:2661-2672(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VTA1 AND VPS4B, MUTAGENESIS OF 158-ASP-GLU-159.
  12. Cited for: INTERACTION WITH IST1; VTA1; VPS4A; MITD1 AND STAMBP.
  13. "Biochemical analyses of human IST1 and its function in cytokinesis."
    Bajorek M., Morita E., Skalicky J.J., Morham S.G., Babst M., Sundquist W.I.
    Mol. Biol. Cell 20:1360-1373(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH IST1.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15."
    Kuang Z., Seo E.J., Leis J.
    J. Virol. 85:7153-7161(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VPS4A.
  16. "Structural basis for midbody targeting of spastin by the ESCRT-III protein CHMP1B."
    Yang D., Rismanchi N., Renvoise B., Lippincott-Schwartz J., Blackstone C., Hurley J.H.
    Nat. Struct. Mol. Biol. 15:1278-1286(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 145-194 IN COMPLEX WITH SPAST, SUBCELLULAR LOCATION, INTERACTION WITH VPS4A, MUTAGENESIS OF THR-178; ALA-181; GLU-184; LEU-188 AND LEU-192.

Entry informationi

Entry nameiCHM1B_HUMAN
AccessioniPrimary (citable) accession number: Q7LBR1
Secondary accession number(s): Q96E89, Q9HD41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3