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Protein

MOB kinase activator 1B

Gene

MOB1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791ZincBy similarity
Metal bindingi84 – 841ZincBy similarity
Metal bindingi161 – 1611ZincBy similarity
Metal bindingi166 – 1661ZincBy similarity

GO - Molecular functioni

  • kinase activator activity Source: UniProtKB
  • kinase binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • hippo signaling Source: UniProtKB
  • positive regulation of phosphorylation Source: GOC
  • protein autophosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_118607. Signaling by Hippo.

Names & Taxonomyi

Protein namesi
Recommended name:
MOB kinase activator 1B
Alternative name(s):
Mob1 homolog 1A
Short name:
Mob1A
Mob1B
Mps one binder kinase activator-like 1A
Gene namesi
Name:MOB1B
Synonyms:MOB4A, MOBKL1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:29801. MOB1B.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134871841.

Polymorphism and mutation databases

BioMutaiMOB1B.
DMDMi56749324.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 216215MOB kinase activator 1BPRO_0000193564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei12 – 121Phosphothreonine; by STK4/MST11 Publication
Modified residuei35 – 351Phosphothreonine; by STK4/MST11 Publication

Post-translational modificationi

Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ7L9L4.
PaxDbiQ7L9L4.
PRIDEiQ7L9L4.

PTM databases

PhosphoSiteiQ7L9L4.

Expressioni

Tissue specificityi

Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver.1 Publication

Gene expression databases

BgeeiQ7L9L4.
CleanExiHS_MOBKL1A.
ExpressionAtlasiQ7L9L4. baseline and differential.
GenevisibleiQ7L9L4. HS.

Organism-specific databases

HPAiHPA047088.

Interactioni

Subunit structurei

Binds STK38L. Interacts with LATS1 and LATS2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC53Q9Y3C03EBI-2558745,EBI-712969
LATS1O958356EBI-2558745,EBI-444209
LATS2Q9NRM73EBI-2558745,EBI-3506895
NT5C2P499023EBI-2558745,EBI-742084
STK4Q130434EBI-2558745,EBI-367376
TNIP1Q150253EBI-2558745,EBI-357849

Protein-protein interaction databases

BioGridi124960. 33 interactions.
IntActiQ7L9L4. 34 interactions.
MINTiMINT-5003307.
STRINGi9606.ENSP00000310189.

Structurei

3D structure databases

ProteinModelPortaliQ7L9L4.
SMRiQ7L9L4. Positions 33-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MOB1/phocein family.Curated

Phylogenomic databases

eggNOGiNOG287143.
GeneTreeiENSGT00550000074456.
HOGENOMiHOG000164685.
HOVERGENiHBG052489.
InParanoidiQ7L9L4.
KOiK06685.
OMAiIRTIFRR.
PhylomeDBiQ7L9L4.
TreeFamiTF300789.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7L9L4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFLFGSRSS KTFKPKKNIP EGSHQYELLK HAEATLGSGN LRMAVMLPEG
60 70 80 90 100
EDLNEWVAVN TVDFFNQINM LYGTITDFCT EESCPVMSAG PKYEYHWADG
110 120 130 140 150
TNIKKPIKCS APKYIDYLMT WVQDQLDDET LFPSKIGVPF PKNFMSVAKT
160 170 180 190 200
ILKRLFRVYA HIYHQHFDPV IQLQEEAHLN TSFKHFIFFV QEFNLIDRRE
210
LAPLQELIEK LTSKDR
Length:216
Mass (Da):25,091
Last modified:January 23, 2007 - v3
Checksum:i29EBA287B0CDAF90
GO
Isoform 2 (identifier: Q7L9L4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MSFLF → MEGATDVNES

Show »
Length:221
Mass (Da):25,499
Checksum:i4DF1286311C0BC5F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 55MSFLF → MEGATDVNES in isoform 2. 1 PublicationVSP_045097

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ577473 mRNA. Translation: CAE12091.1.
AK299481 mRNA. Translation: BAG61445.1.
AK313513 mRNA. Translation: BAG36293.1.
AC021989 Genomic DNA. No translation available.
AC093851 Genomic DNA. No translation available.
AC108014 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05636.1.
BC038112 mRNA. Translation: AAH38112.1.
CCDSiCCDS34002.1. [Q7L9L4-1]
CCDS58903.1. [Q7L9L4-2]
RefSeqiNP_001231695.1. NM_001244766.1. [Q7L9L4-2]
NP_775739.1. NM_173468.3. [Q7L9L4-1]
XP_005265766.1. XM_005265709.2. [Q7L9L4-2]
UniGeneiHs.691454.
Hs.744715.

Genome annotation databases

EnsembliENST00000309395; ENSP00000310189; ENSG00000173542.
ENST00000396051; ENSP00000379366; ENSG00000173542. [Q7L9L4-2]
GeneIDi92597.
KEGGihsa:92597.
UCSCiuc003hfw.3. human. [Q7L9L4-1]
uc011cba.2. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ577473 mRNA. Translation: CAE12091.1.
AK299481 mRNA. Translation: BAG61445.1.
AK313513 mRNA. Translation: BAG36293.1.
AC021989 Genomic DNA. No translation available.
AC093851 Genomic DNA. No translation available.
AC108014 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05636.1.
BC038112 mRNA. Translation: AAH38112.1.
CCDSiCCDS34002.1. [Q7L9L4-1]
CCDS58903.1. [Q7L9L4-2]
RefSeqiNP_001231695.1. NM_001244766.1. [Q7L9L4-2]
NP_775739.1. NM_173468.3. [Q7L9L4-1]
XP_005265766.1. XM_005265709.2. [Q7L9L4-2]
UniGeneiHs.691454.
Hs.744715.

3D structure databases

ProteinModelPortaliQ7L9L4.
SMRiQ7L9L4. Positions 33-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124960. 33 interactions.
IntActiQ7L9L4. 34 interactions.
MINTiMINT-5003307.
STRINGi9606.ENSP00000310189.

PTM databases

PhosphoSiteiQ7L9L4.

Polymorphism and mutation databases

BioMutaiMOB1B.
DMDMi56749324.

Proteomic databases

MaxQBiQ7L9L4.
PaxDbiQ7L9L4.
PRIDEiQ7L9L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309395; ENSP00000310189; ENSG00000173542.
ENST00000396051; ENSP00000379366; ENSG00000173542. [Q7L9L4-2]
GeneIDi92597.
KEGGihsa:92597.
UCSCiuc003hfw.3. human. [Q7L9L4-1]
uc011cba.2. human.

Organism-specific databases

CTDi92597.
GeneCardsiGC04P071769.
HGNCiHGNC:29801. MOB1B.
HPAiHPA047088.
MIMi609282. gene.
neXtProtiNX_Q7L9L4.
PharmGKBiPA134871841.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG287143.
GeneTreeiENSGT00550000074456.
HOGENOMiHOG000164685.
HOVERGENiHBG052489.
InParanoidiQ7L9L4.
KOiK06685.
OMAiIRTIFRR.
PhylomeDBiQ7L9L4.
TreeFamiTF300789.

Enzyme and pathway databases

ReactomeiREACT_118607. Signaling by Hippo.

Miscellaneous databases

ChiTaRSiMOB1B. human.
GeneWikiiMOBKL1A.
GenomeRNAii92597.
NextBioi77815.
PROiQ7L9L4.
SOURCEiSearch...

Gene expression databases

BgeeiQ7L9L4.
CleanExiHS_MOBKL1A.
ExpressionAtlasiQ7L9L4. baseline and differential.
GenevisibleiQ7L9L4. HS.

Family and domain databases

Gene3Di1.20.140.30. 1 hit.
InterProiIPR005301. MOB_kinase_act_fam.
[Graphical view]
PANTHERiPTHR22599. PTHR22599. 1 hit.
PfamiPF03637. Mob1_phocein. 1 hit.
[Graphical view]
SUPFAMiSSF101152. SSF101152. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the human Mob-1 like proteins."
    Florindo C.S., Tavares A.A.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases."
    Devroe E., Erdjument-Bromage H., Tempst P., Silver P.A.
    J. Biol. Chem. 279:24444-24451(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STK38L, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  6. "MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation."
    Praskova M., Xia F., Avruch J.
    Curr. Biol. 18:311-321(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-12 AND THR-35.
  7. "Molecular characterization of human homologs of yeast MOB1."
    Chow A., Hao Y., Yang X.
    Int. J. Cancer 126:2079-2089(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LATS1 AND LATS2, TISSUE SPECIFICITY.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMOB1B_HUMAN
AccessioniPrimary (citable) accession number: Q7L9L4
Secondary accession number(s): B2R8U6, B4DRY3, Q8IY23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.