Q7L9L4 (MOB1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MOB kinase activator 1B

Alternative name(s):
Mob1 homolog 1A
Short name=Mob1A
Mob1B
Mps one binder kinase activator-like 1A

Gene names

Name:	MOB1B
Synonyms:	MOB4A, MOBKL1A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L. Ref.5 Ref.7
Subunit structure	Binds STK38L. Interacts with LATS1 and LATS2. Ref.5 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.5.
Tissue specificity	Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver. Ref.7
Post-translational modification	Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1. Ref.6
Sequence similarities	Belongs to the MOB1/phocein family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hippo signaling cascade Inferred from direct assay Ref.7. Source: UniProtKB protein autophosphorylation Inferred from direct assay PubMed 15197186. Source: UniProtKB
Cellular_component	cytosol Traceable author statement. Source: Reactome nucleus Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	kinase activator activity Inferred from direct assay PubMed 15197186. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LATS1	O95835	2	EBI-2558745,EBI-444209
LATS2	Q9NRM7	2	EBI-2558745,EBI-3506895

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7L9L4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7L9L4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MSFLF → MEGATDVNES

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	MOB kinase activator 1B		PRO_0000193564
Sites
	Metal binding	79	1	Zinc By similarity
	Metal binding	84	1	Zinc By similarity
	Metal binding	161	1	Zinc By similarity
	Metal binding	166	1	Zinc By similarity
Amino acid modifications
	Modified residue	12	1	Phosphothreonine; by STK4/MST1 Ref.6
	Modified residue	35	1	Phosphothreonine; by STK4/MST1 Ref.6
Natural variations
	Alternative sequence	1 – 5	5	MSFLF → MEGATDVNES in isoform 2.		VSP_045097

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 29EBA287B0CDAF90	FASTA	216	25,091
	10 20 30 40 50 60 MSFLFGSRSS KTFKPKKNIP EGSHQYELLK HAEATLGSGN LRMAVMLPEG EDLNEWVAVN 70 80 90 100 110 120 TVDFFNQINM LYGTITDFCT EESCPVMSAG PKYEYHWADG TNIKKPIKCS APKYIDYLMT 130 140 150 160 170 180 WVQDQLDDET LFPSKIGVPF PKNFMSVAKT ILKRLFRVYA HIYHQHFDPV IQLQEEAHLN 190 200 210 TSFKHFIFFV QEFNLIDRRE LAPLQELIEK LTSKDR
	Isoform 2 [UniParc]. Checksum: 4DF1286311C0BC5F	FASTA	221	25,499
	10 20 30 40 50 60 MEGATDVNES GSRSSKTFKP KKNIPEGSHQ YELLKHAEAT LGSGNLRMAV MLPEGEDLNE 70 80 90 100 110 120 WVAVNTVDFF NQINMLYGTI TDFCTEESCP VMSAGPKYEY HWADGTNIKK PIKCSAPKYI 130 140 150 160 170 180 DYLMTWVQDQ LDDETLFPSK IGVPFPKNFM SVAKTILKRL FRVYAHIYHQ HFDPVIQLQE 190 200 210 220 EAHLNTSFKH FIFFVQEFNL IDRRELAPLQ ELIEKLTSKD R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human Mob-1 like proteins." Florindo C.S., Tavares A.A. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases." Devroe E., Erdjument-Bromage H., Tempst P., Silver P.A. J. Biol. Chem. 279:24444-24451(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH STK38L, MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[6]	"MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation." Praskova M., Xia F., Avruch J. Curr. Biol. 18:311-321(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-12 AND THR-35.
[7]	"Molecular characterization of human homologs of yeast MOB1." Chow A., Hao Y., Yang X. Int. J. Cancer 126:2079-2089(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH LATS1 AND LATS2, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ577473 mRNA. Translation: CAE12091.1. AK299481 mRNA. Translation: BAG61445.1. AK313513 mRNA. Translation: BAG36293.1. AC021989 Genomic DNA. No translation available. AC093851 Genomic DNA. No translation available. AC108014 Genomic DNA. No translation available. CH471057 Genomic DNA. Translation: EAX05636.1. BC038112 mRNA. Translation: AAH38112.1.
IPI	IPI00514212.
RefSeq	NP_001231695.1. NM_001244766.1. NP_775739.1. NM_173468.3.
UniGene	Hs.691454. Hs.744715.
3D structure databases
ProteinModelPortal	Q7L9L4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7L9L4. 6 interactions.
MINT	MINT-5003307.
STRING	9606.ENSP00000310189.
PTM databases
PhosphoSite	Q7L9L4.
Polymorphism databases
DMDM	56749324.
Proteomic databases
PaxDb	Q7L9L4.
PRIDE	Q7L9L4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000309395; ENSP00000310189; ENSG00000173542. ENST00000396051; ENSP00000379366; ENSG00000173542.
GeneID	92597.
KEGG	hsa:92597.
UCSC	uc003hfw.3. human. uc011cba.2. human.
Organism-specific databases
CTD	92597.
GeneCards	GC04P071769.
HGNC	HGNC:29801. MOB1B.
MIM	609282. gene.
neXtProt	NX_Q7L9L4.
PharmGKB	PA134871841.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG287143.
HOGENOM	HOG000164685.
HOVERGEN	HBG052489.
InParanoid	Q7L9L4.
KO	K06685.
OMA	NLIERRE.
OrthoDB	EOG42822G.
PhylomeDB	Q7L9L4.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q7L9L4.
Bgee	Q7L9L4.
CleanEx	HS_MOBKL1A.
Genevestigator	Q7L9L4.
GermOnline	ENSG00000173542. Homo sapiens.
Family and domain databases
Gene3D	1.20.140.30. 1 hit.
InterPro	IPR005301. Mob1_phocein. [Graphical view]
PANTHER	PTHR22599. PTHR22599. 1 hit.
Pfam	PF03637. Mob1_phocein. 1 hit. [Graphical view]
SUPFAM	SSF101152. Mob1_phocein. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	92597.
NextBio	77815.
SOURCE	Search...

Entry information

Entry name

MOB1B_HUMAN

Accession

Primary (citable) accession number: Q7L9L4
Secondary accession number(s): B2R8U6, B4DRY3, Q8IY23

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.