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Protein

OTU domain-containing protein 6A

Gene

OTUD6A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that hydrolyzes 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491By similarity
Active sitei152 – 1521NucleophileBy similarity
Active sitei268 – 2681By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. protein K11-linked deubiquitination Source: UniProtKB
  2. protein K27-linked deubiquitination Source: UniProtKB
  3. protein K29-linked deubiquitination Source: UniProtKB
  4. protein K33-linked deubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 6A (EC:3.4.19.12)
Alternative name(s):
DUBA-2
Gene namesi
Name:OTUD6A
Synonyms:DUBA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:32312. OTUD6A.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671218.

Polymorphism and mutation databases

BioMutaiOTUD6A.
DMDMi74713135.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288OTU domain-containing protein 6APRO_0000076277Add
BLAST

Proteomic databases

PaxDbiQ7L8S5.
PRIDEiQ7L8S5.

PTM databases

PhosphoSiteiQ7L8S5.

Expressioni

Gene expression databases

BgeeiQ7L8S5.
CleanExiHS_OTUD6A.
GenevestigatoriQ7L8S5.

Organism-specific databases

HPAiHPA053304.

Interactioni

Protein-protein interaction databases

BioGridi126574. 7 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ7L8S5.
SMRiQ7L8S5. Positions 122-272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini141 – 275135OTUPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni146 – 1527Cys-loopBy similarity
Regioni210 – 22011Variable-loopBy similarityAdd
BLAST
Regioni258 – 26811His-loopBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000012840.
HOGENOMiHOG000192906.
HOVERGENiHBG080227.
InParanoidiQ7L8S5.
KOiK18342.
OMAiMRKHVDD.
OrthoDBiEOG7WT42W.
PhylomeDBiQ7L8S5.
TreeFamiTF315010.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7L8S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDPKSEQQR ILRRHQRERQ ELQAQIRSLK NSVPKTDKTK RKQLLQDVAR
60 70 80 90 100
MEAEMAQKHR QELEKFQDDS SIESVVEDLA KMNLENRPPR SSKAHRKRER
110 120 130 140 150
MESEERERQE SIFQAEMSEH LAGFKREEEE KLAAILGARG LEMKAIPADG
160 170 180 190 200
HCMYRAIQDQ LVFSVSVEML RCRTASYMKK HVDEFLPFFS NPETSDSFGY
210 220 230 240 250
DDFMIYCDNI VRTTAWGGQL ELRALSHVLK TPIEVIQADS PTLIIGEEYV
260 270 280
KKPIILVYLR YAYSLGEHYN SVTPLEAGAA GGVLPRLL
Length:288
Mass (Da):33,300
Last modified:February 15, 2005 - v1
Checksum:i78DCB2729A729C31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098697 mRNA. Translation: BAC05384.1.
CH471132 Genomic DNA. Translation: EAX05357.1.
BC137355 mRNA. Translation: AAI37356.1.
BC137356 mRNA. Translation: AAI37357.1.
CCDSiCCDS14395.1.
RefSeqiNP_997203.1. NM_207320.2.
UniGeneiHs.447381.

Genome annotation databases

EnsembliENST00000338352; ENSP00000339389; ENSG00000189401.
GeneIDi139562.
KEGGihsa:139562.
UCSCiuc004dxu.1. human.

Polymorphism and mutation databases

BioMutaiOTUD6A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098697 mRNA. Translation: BAC05384.1.
CH471132 Genomic DNA. Translation: EAX05357.1.
BC137355 mRNA. Translation: AAI37356.1.
BC137356 mRNA. Translation: AAI37357.1.
CCDSiCCDS14395.1.
RefSeqiNP_997203.1. NM_207320.2.
UniGeneiHs.447381.

3D structure databases

ProteinModelPortaliQ7L8S5.
SMRiQ7L8S5. Positions 122-272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126574. 7 interactions.

PTM databases

PhosphoSiteiQ7L8S5.

Polymorphism and mutation databases

BioMutaiOTUD6A.
DMDMi74713135.

Proteomic databases

PaxDbiQ7L8S5.
PRIDEiQ7L8S5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338352; ENSP00000339389; ENSG00000189401.
GeneIDi139562.
KEGGihsa:139562.
UCSCiuc004dxu.1. human.

Organism-specific databases

CTDi139562.
GeneCardsiGC0XP069282.
HGNCiHGNC:32312. OTUD6A.
HPAiHPA053304.
MIMi300714. gene.
neXtProtiNX_Q7L8S5.
PharmGKBiPA142671218.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000012840.
HOGENOMiHOG000192906.
HOVERGENiHBG080227.
InParanoidiQ7L8S5.
KOiK18342.
OMAiMRKHVDD.
OrthoDBiEOG7WT42W.
PhylomeDBiQ7L8S5.
TreeFamiTF315010.

Miscellaneous databases

GenomeRNAii139562.
NextBioi83989.
PROiQ7L8S5.
SOURCEiSearch...

Gene expression databases

BgeeiQ7L8S5.
CleanExiHS_OTUD6A.
GenevestigatoriQ7L8S5.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis."
    Mevissen T.E., Hospenthal M.K., Geurink P.P., Elliott P.R., Akutsu M., Arnaudo N., Ekkebus R., Kulathu Y., Wauer T., El Oualid F., Freund S.M., Ovaa H., Komander D.
    Cell 154:169-184(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiOTU6A_HUMAN
AccessioniPrimary (citable) accession number: Q7L8S5
Secondary accession number(s): B2RPB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: February 15, 2005
Last modified: April 29, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.