SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7L8A9

- VASH1_HUMAN

UniProt

Q7L8A9 - VASH1_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Vasohibin-1
Gene
VASH1, KIAA1036, VASH
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei29 – 302Cleavage Inferred
Sitei76 – 772Cleavage Inferred

GO - Biological processi

  1. angiogenesis Source: MGI
  2. cell cycle arrest Source: UniProtKB-KW
  3. negative regulation of angiogenesis Source: UniProtKB
  4. negative regulation of blood vessel endothelial cell migration Source: UniProtKB
  5. negative regulation of endothelial cell migration Source: MGI
  6. negative regulation of endothelial cell proliferation Source: UniProtKB
  7. negative regulation of lymphangiogenesis Source: MGI
  8. regulation of cellular senescence Source: MGI
  9. response to wounding Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Growth arrest

Names & Taxonomyi

Protein namesi
Recommended name:
Vasohibin-1
Gene namesi
Name:VASH1
Synonyms:KIAA1036, VASH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:19964. VASH1.

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. apical part of cell Source: MGI
  2. cytoplasm Source: MGI
  3. endoplasmic reticulum Source: UniProtKB
  4. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi29 – 291R → A: Disappearance of 42 kDa processed form. 1 Publication
Mutagenesisi76 – 761R → A: Disappearance of 36, 32 and 27 kDa processed forms. 1 Publication

Organism-specific databases

PharmGKBiPA134941450.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Vasohibin-1
PRO_0000189980Add
BLAST

Post-translational modificationi

2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.

Proteomic databases

PaxDbiQ7L8A9.
PRIDEiQ7L8A9.

PTM databases

PhosphoSiteiQ7L8A9.

Expressioni

Tissue specificityi

Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.2 Publications

Inductioni

By VEGF.2 Publications

Gene expression databases

ArrayExpressiQ7L8A9.
BgeeiQ7L8A9.
CleanExiHS_VASH1.
GenevestigatoriQ7L8A9.

Organism-specific databases

HPAiHPA000653.

Interactioni

Protein-protein interaction databases

BioGridi116518. 2 interactions.
STRINGi9606.ENSP00000167106.

Structurei

3D structure databases

ProteinModelPortaliQ7L8A9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni319 – 36547Involved in heparin-binding and antiangiogenic activity
Add
BLAST

Sequence similaritiesi

Belongs to the vasohibin family.

Phylogenomic databases

eggNOGiNOG41266.
HOGENOMiHOG000007477.
HOVERGENiHBG079337.
InParanoidiQ7L8A9.
OMAiWERMWRH.
OrthoDBiEOG7DC24N.
PhylomeDBiQ7L8A9.
TreeFamiTF329370.

Family and domain databases

InterProiIPR028131. Vasohibin.
IPR028132. Vasohibin-1.
[Graphical view]
PANTHERiPTHR15750:SF3. PTHR15750:SF3. 1 hit.
PfamiPF14822. Vasohibin. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7L8A9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPGGKKVAGG GSSGATPTSA AATAPSGVRR LETSEGTSAQ RDEEPEEEGE    50
EDLRDGGVPF FVNRGGLPVD EATWERMWKH VAKIHPDGEK VAQRIRGATD 100
LPKIPIPSVP TFQPSTPVPE RLEAVQRYIR ELQYNHTGTQ FFEIKKSRPL 150
TGLMDLAKEM TKEALPIKCL EAVILGIYLT NSMPTLERFP ISFKTYFSGN 200
YFRHIVLGVN FAGRYGALGM SRREDLMYKP PAFRTLSELV LDFEAAYGRC 250
WHVLKKVKLG QSVSHDPHSV EQIEWKHSVL DVERLGRDDF RKELERHARD 300
MRLKIGKGTG PPSPTKDRKK DVSSPQRAQS SPHRRNSRSE RRPSGDKKTS 350
EPKAMPDLNG YQIRV 365
Length:365
Mass (Da):40,957
Last modified:July 5, 2004 - v1
Checksum:iCEE39875FA9DA70B
GO
Isoform 2 (identifier: Q7L8A9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     177-204: IYLTNSMPTLERFPISFKTYFSGNYFRH → MYPSSPEGEGSGLLWASASCSESEGGVG
     205-365: Missing.

Show »
Length:204
Mass (Da):21,845
Checksum:iCE4652CF401BBA85
GO

Sequence cautioni

The sequence BAA82988.2 differs from that shown. Reason: Erroneous initiation.
The sequence AAD44361.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei177 – 20428IYLTN…NYFRH → MYPSSPEGEGSGLLWASASC SESEGGVG in isoform 2.
VSP_013324Add
BLAST
Alternative sequencei205 – 365161Missing in isoform 2.
VSP_013325Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB028959 mRNA. Translation: BAA82988.2. Different initiation.
AL832588 mRNA. Translation: CAD89941.1.
AC007376 Genomic DNA. Translation: AAF02829.1.
AF111169 Genomic DNA. Translation: AAD44361.1. Sequence problems.
BC009031 mRNA. Translation: AAH09031.1.
BC051896 mRNA. Translation: AAH51896.1.
CCDSiCCDS9851.1. [Q7L8A9-1]
RefSeqiNP_055724.1. NM_014909.4. [Q7L8A9-1]
UniGeneiHs.525479.

Genome annotation databases

EnsembliENST00000167106; ENSP00000167106; ENSG00000071246. [Q7L8A9-1]
ENST00000554237; ENSP00000451613; ENSG00000071246. [Q7L8A9-2]
GeneIDi22846.
KEGGihsa:22846.
UCSCiuc001xss.3. human. [Q7L8A9-2]
uc001xst.2. human. [Q7L8A9-1]

Polymorphism databases

DMDMi62511163.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB028959 mRNA. Translation: BAA82988.2 . Different initiation.
AL832588 mRNA. Translation: CAD89941.1 .
AC007376 Genomic DNA. Translation: AAF02829.1 .
AF111169 Genomic DNA. Translation: AAD44361.1 . Sequence problems.
BC009031 mRNA. Translation: AAH09031.1 .
BC051896 mRNA. Translation: AAH51896.1 .
CCDSi CCDS9851.1. [Q7L8A9-1 ]
RefSeqi NP_055724.1. NM_014909.4. [Q7L8A9-1 ]
UniGenei Hs.525479.

3D structure databases

ProteinModelPortali Q7L8A9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116518. 2 interactions.
STRINGi 9606.ENSP00000167106.

PTM databases

PhosphoSitei Q7L8A9.

Polymorphism databases

DMDMi 62511163.

Proteomic databases

PaxDbi Q7L8A9.
PRIDEi Q7L8A9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000167106 ; ENSP00000167106 ; ENSG00000071246 . [Q7L8A9-1 ]
ENST00000554237 ; ENSP00000451613 ; ENSG00000071246 . [Q7L8A9-2 ]
GeneIDi 22846.
KEGGi hsa:22846.
UCSCi uc001xss.3. human. [Q7L8A9-2 ]
uc001xst.2. human. [Q7L8A9-1 ]

Organism-specific databases

CTDi 22846.
GeneCardsi GC14P077228.
HGNCi HGNC:19964. VASH1.
HPAi HPA000653.
MIMi 609011. gene.
neXtProti NX_Q7L8A9.
PharmGKBi PA134941450.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG41266.
HOGENOMi HOG000007477.
HOVERGENi HBG079337.
InParanoidi Q7L8A9.
OMAi WERMWRH.
OrthoDBi EOG7DC24N.
PhylomeDBi Q7L8A9.
TreeFami TF329370.

Miscellaneous databases

ChiTaRSi VASH1. human.
GeneWikii VASH1.
GenomeRNAii 22846.
NextBioi 43305.
PROi Q7L8A9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q7L8A9.
Bgeei Q7L8A9.
CleanExi HS_VASH1.
Genevestigatori Q7L8A9.

Family and domain databases

InterProi IPR028131. Vasohibin.
IPR028132. Vasohibin-1.
[Graphical view ]
PANTHERi PTHR15750:SF3. PTHR15750:SF3. 1 hit.
Pfami PF14822. Vasohibin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Spinal cord.
  3. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  5. "Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis."
    Watanabe K., Hasegawa Y., Yamashita H., Shimizu K., Ding Y., Abe M., Ohta H., Imagawa K., Hojo K., Maki H., Sonoda H., Sato Y.
    J. Clin. Invest. 114:898-907(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  6. "Gene regulation of a novel angiogenesis inhibitor, vasohibin, in endothelial cells."
    Shimizu K., Watanabe K., Yamashita H., Abe M., Yoshimatsu H., Ohta H., Sonoda H., Sato Y.
    Biochem. Biophys. Res. Commun. 327:700-706(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, INDUCTION.
  7. "Multiple processing forms and their biological activities of a novel angiogenesis inhibitor vasohibin."
    Sonoda H., Ohta H., Watanabe K., Yamashita H., Kimura H., Sato Y.
    Biochem. Biophys. Res. Commun. 342:640-646(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CLEAVAGE SITE, REGION, MUTAGENESIS OF ARG-29 AND ARG-76.
  8. "Vasohibin prevents arterial neointimal formation through angiogenesis inhibition."
    Yamashita H., Abe M., Watanabe K., Shimizu K., Moriya T., Sato A., Satomi S., Ohta H., Sonoda H., Sato Y.
    Biochem. Biophys. Res. Commun. 345:919-925(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiVASH1_HUMAN
AccessioniPrimary (citable) accession number: Q7L8A9
Secondary accession number(s): Q96H02, Q9UBF4, Q9Y629
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although probably secreted, it lacks a canonical signal sequence.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi