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Q7L8A9

- VASH1_HUMAN

UniProt

Q7L8A9 - VASH1_HUMAN

Protein

Vasohibin-1

Gene

VASH1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei29 – 302CleavageCurated
    Sitei76 – 772CleavageCurated

    GO - Biological processi

    1. angiogenesis Source: MGI
    2. cell cycle arrest Source: UniProtKB-KW
    3. negative regulation of angiogenesis Source: UniProtKB
    4. negative regulation of blood vessel endothelial cell migration Source: UniProtKB
    5. negative regulation of endothelial cell migration Source: MGI
    6. negative regulation of endothelial cell proliferation Source: UniProtKB
    7. negative regulation of lymphangiogenesis Source: MGI
    8. regulation of cellular senescence Source: MGI
    9. response to wounding Source: MGI

    Keywords - Biological processi

    Cell cycle, Growth arrest

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vasohibin-1
    Gene namesi
    Name:VASH1
    Synonyms:KIAA1036, VASH
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:19964. VASH1.

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. apical part of cell Source: MGI
    2. cytoplasm Source: MGI
    3. endoplasmic reticulum Source: UniProtKB
    4. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi29 – 291R → A: Disappearance of 42 kDa processed form. 1 Publication
    Mutagenesisi76 – 761R → A: Disappearance of 36, 32 and 27 kDa processed forms. 1 Publication

    Organism-specific databases

    PharmGKBiPA134941450.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365Vasohibin-1PRO_0000189980Add
    BLAST

    Post-translational modificationi

    2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.1 Publication

    Proteomic databases

    PaxDbiQ7L8A9.
    PRIDEiQ7L8A9.

    PTM databases

    PhosphoSiteiQ7L8A9.

    Expressioni

    Tissue specificityi

    Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.2 Publications

    Inductioni

    By VEGF.2 Publications

    Gene expression databases

    ArrayExpressiQ7L8A9.
    BgeeiQ7L8A9.
    CleanExiHS_VASH1.
    GenevestigatoriQ7L8A9.

    Organism-specific databases

    HPAiHPA000653.

    Interactioni

    Protein-protein interaction databases

    BioGridi116518. 2 interactions.
    STRINGi9606.ENSP00000167106.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7L8A9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni319 – 36547Involved in heparin-binding and antiangiogenic activityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the vasohibin family.Curated

    Phylogenomic databases

    eggNOGiNOG41266.
    HOGENOMiHOG000007477.
    HOVERGENiHBG079337.
    InParanoidiQ7L8A9.
    OMAiWERMWRH.
    OrthoDBiEOG7DC24N.
    PhylomeDBiQ7L8A9.
    TreeFamiTF329370.

    Family and domain databases

    InterProiIPR028131. Vasohibin.
    IPR028132. Vasohibin-1.
    [Graphical view]
    PANTHERiPTHR15750:SF3. PTHR15750:SF3. 1 hit.
    PfamiPF14822. Vasohibin. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q7L8A9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPGGKKVAGG GSSGATPTSA AATAPSGVRR LETSEGTSAQ RDEEPEEEGE    50
    EDLRDGGVPF FVNRGGLPVD EATWERMWKH VAKIHPDGEK VAQRIRGATD 100
    LPKIPIPSVP TFQPSTPVPE RLEAVQRYIR ELQYNHTGTQ FFEIKKSRPL 150
    TGLMDLAKEM TKEALPIKCL EAVILGIYLT NSMPTLERFP ISFKTYFSGN 200
    YFRHIVLGVN FAGRYGALGM SRREDLMYKP PAFRTLSELV LDFEAAYGRC 250
    WHVLKKVKLG QSVSHDPHSV EQIEWKHSVL DVERLGRDDF RKELERHARD 300
    MRLKIGKGTG PPSPTKDRKK DVSSPQRAQS SPHRRNSRSE RRPSGDKKTS 350
    EPKAMPDLNG YQIRV 365
    Length:365
    Mass (Da):40,957
    Last modified:July 5, 2004 - v1
    Checksum:iCEE39875FA9DA70B
    GO
    Isoform 2 (identifier: Q7L8A9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         177-204: IYLTNSMPTLERFPISFKTYFSGNYFRH → MYPSSPEGEGSGLLWASASCSESEGGVG
         205-365: Missing.

    Show »
    Length:204
    Mass (Da):21,845
    Checksum:iCE4652CF401BBA85
    GO

    Sequence cautioni

    The sequence BAA82988.2 differs from that shown. Reason: Erroneous initiation.
    The sequence AAD44361.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei177 – 20428IYLTN…NYFRH → MYPSSPEGEGSGLLWASASC SESEGGVG in isoform 2. 1 PublicationVSP_013324Add
    BLAST
    Alternative sequencei205 – 365161Missing in isoform 2. 1 PublicationVSP_013325Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028959 mRNA. Translation: BAA82988.2. Different initiation.
    AL832588 mRNA. Translation: CAD89941.1.
    AC007376 Genomic DNA. Translation: AAF02829.1.
    AF111169 Genomic DNA. Translation: AAD44361.1. Sequence problems.
    BC009031 mRNA. Translation: AAH09031.1.
    BC051896 mRNA. Translation: AAH51896.1.
    CCDSiCCDS9851.1. [Q7L8A9-1]
    RefSeqiNP_055724.1. NM_014909.4. [Q7L8A9-1]
    UniGeneiHs.525479.

    Genome annotation databases

    EnsembliENST00000167106; ENSP00000167106; ENSG00000071246. [Q7L8A9-1]
    ENST00000554237; ENSP00000451613; ENSG00000071246. [Q7L8A9-2]
    GeneIDi22846.
    KEGGihsa:22846.
    UCSCiuc001xss.3. human. [Q7L8A9-2]
    uc001xst.2. human. [Q7L8A9-1]

    Polymorphism databases

    DMDMi62511163.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028959 mRNA. Translation: BAA82988.2 . Different initiation.
    AL832588 mRNA. Translation: CAD89941.1 .
    AC007376 Genomic DNA. Translation: AAF02829.1 .
    AF111169 Genomic DNA. Translation: AAD44361.1 . Sequence problems.
    BC009031 mRNA. Translation: AAH09031.1 .
    BC051896 mRNA. Translation: AAH51896.1 .
    CCDSi CCDS9851.1. [Q7L8A9-1 ]
    RefSeqi NP_055724.1. NM_014909.4. [Q7L8A9-1 ]
    UniGenei Hs.525479.

    3D structure databases

    ProteinModelPortali Q7L8A9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116518. 2 interactions.
    STRINGi 9606.ENSP00000167106.

    PTM databases

    PhosphoSitei Q7L8A9.

    Polymorphism databases

    DMDMi 62511163.

    Proteomic databases

    PaxDbi Q7L8A9.
    PRIDEi Q7L8A9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000167106 ; ENSP00000167106 ; ENSG00000071246 . [Q7L8A9-1 ]
    ENST00000554237 ; ENSP00000451613 ; ENSG00000071246 . [Q7L8A9-2 ]
    GeneIDi 22846.
    KEGGi hsa:22846.
    UCSCi uc001xss.3. human. [Q7L8A9-2 ]
    uc001xst.2. human. [Q7L8A9-1 ]

    Organism-specific databases

    CTDi 22846.
    GeneCardsi GC14P077228.
    HGNCi HGNC:19964. VASH1.
    HPAi HPA000653.
    MIMi 609011. gene.
    neXtProti NX_Q7L8A9.
    PharmGKBi PA134941450.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG41266.
    HOGENOMi HOG000007477.
    HOVERGENi HBG079337.
    InParanoidi Q7L8A9.
    OMAi WERMWRH.
    OrthoDBi EOG7DC24N.
    PhylomeDBi Q7L8A9.
    TreeFami TF329370.

    Miscellaneous databases

    ChiTaRSi VASH1. human.
    GeneWikii VASH1.
    GenomeRNAii 22846.
    NextBioi 43305.
    PROi Q7L8A9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q7L8A9.
    Bgeei Q7L8A9.
    CleanExi HS_VASH1.
    Genevestigatori Q7L8A9.

    Family and domain databases

    InterProi IPR028131. Vasohibin.
    IPR028132. Vasohibin-1.
    [Graphical view ]
    PANTHERi PTHR15750:SF3. PTHR15750:SF3. 1 hit.
    Pfami PF14822. Vasohibin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Spinal cord.
    3. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain.
    5. "Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis."
      Watanabe K., Hasegawa Y., Yamashita H., Shimizu K., Ding Y., Abe M., Ohta H., Imagawa K., Hojo K., Maki H., Sonoda H., Sato Y.
      J. Clin. Invest. 114:898-907(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    6. "Gene regulation of a novel angiogenesis inhibitor, vasohibin, in endothelial cells."
      Shimizu K., Watanabe K., Yamashita H., Abe M., Yoshimatsu H., Ohta H., Sonoda H., Sato Y.
      Biochem. Biophys. Res. Commun. 327:700-706(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING, INDUCTION.
    7. "Multiple processing forms and their biological activities of a novel angiogenesis inhibitor vasohibin."
      Sonoda H., Ohta H., Watanabe K., Yamashita H., Kimura H., Sato Y.
      Biochem. Biophys. Res. Commun. 342:640-646(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CLEAVAGE SITE, REGION, MUTAGENESIS OF ARG-29 AND ARG-76.
    8. "Vasohibin prevents arterial neointimal formation through angiogenesis inhibition."
      Yamashita H., Abe M., Watanabe K., Shimizu K., Moriya T., Sato A., Satomi S., Ohta H., Sonoda H., Sato Y.
      Biochem. Biophys. Res. Commun. 345:919-925(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiVASH1_HUMAN
    AccessioniPrimary (citable) accession number: Q7L8A9
    Secondary accession number(s): Q96H02, Q9UBF4, Q9Y629
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Although probably secreted, it lacks a canonical signal sequence.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3