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Q7L8A9 (VASH1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vasohibin-1
Gene names
Name:VASH1
Synonyms:KIAA1036, VASH
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity. Ref.5 Ref.7 Ref.8

Subcellular location

Secreted Ref.5 Ref.8.

Tissue specificity

Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions. Ref.5 Ref.8

Induction

By VEGF. Ref.5 Ref.6

Post-translational modification

2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.

Sequence similarities

Belongs to the vasohibin family.

Caution

Although probably secreted, it lacks a canonical signal sequence.

Sequence caution

The sequence AAD44361.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAA82988.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7L8A9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7L8A9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     177-204: IYLTNSMPTLERFPISFKTYFSGNYFRH → MYPSSPEGEGSGLLWASASCSESEGGVG
     205-365: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Vasohibin-1
PRO_0000189980

Regions

Region319 – 36547Involved in heparin-binding and antiangiogenic activity

Sites

Site29 – 302Cleavage Probable
Site76 – 772Cleavage Probable

Natural variations

Alternative sequence177 – 20428IYLTN…NYFRH → MYPSSPEGEGSGLLWASASC SESEGGVG in isoform 2.
VSP_013324
Alternative sequence205 – 365161Missing in isoform 2.
VSP_013325

Experimental info

Mutagenesis291R → A: Disappearance of 42 kDa processed form. Ref.7
Mutagenesis761R → A: Disappearance of 36, 32 and 27 kDa processed forms. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: CEE39875FA9DA70B

FASTA36540,957
        10         20         30         40         50         60 
MPGGKKVAGG GSSGATPTSA AATAPSGVRR LETSEGTSAQ RDEEPEEEGE EDLRDGGVPF 

        70         80         90        100        110        120 
FVNRGGLPVD EATWERMWKH VAKIHPDGEK VAQRIRGATD LPKIPIPSVP TFQPSTPVPE 

       130        140        150        160        170        180 
RLEAVQRYIR ELQYNHTGTQ FFEIKKSRPL TGLMDLAKEM TKEALPIKCL EAVILGIYLT 

       190        200        210        220        230        240 
NSMPTLERFP ISFKTYFSGN YFRHIVLGVN FAGRYGALGM SRREDLMYKP PAFRTLSELV 

       250        260        270        280        290        300 
LDFEAAYGRC WHVLKKVKLG QSVSHDPHSV EQIEWKHSVL DVERLGRDDF RKELERHARD 

       310        320        330        340        350        360 
MRLKIGKGTG PPSPTKDRKK DVSSPQRAQS SPHRRNSRSE RRPSGDKKTS EPKAMPDLNG 


YQIRV 

« Hide

Isoform 2 [UniParc].

Checksum: CE4652CF401BBA85
Show »

FASTA20421,845

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Spinal cord.
[3]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[5]"Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis."
Watanabe K., Hasegawa Y., Yamashita H., Shimizu K., Ding Y., Abe M., Ohta H., Imagawa K., Hojo K., Maki H., Sonoda H., Sato Y.
J. Clin. Invest. 114:898-907(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[6]"Gene regulation of a novel angiogenesis inhibitor, vasohibin, in endothelial cells."
Shimizu K., Watanabe K., Yamashita H., Abe M., Yoshimatsu H., Ohta H., Sonoda H., Sato Y.
Biochem. Biophys. Res. Commun. 327:700-706(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, INDUCTION.
[7]"Multiple processing forms and their biological activities of a novel angiogenesis inhibitor vasohibin."
Sonoda H., Ohta H., Watanabe K., Yamashita H., Kimura H., Sato Y.
Biochem. Biophys. Res. Commun. 342:640-646(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CLEAVAGE SITE, REGION, MUTAGENESIS OF ARG-29 AND ARG-76.
[8]"Vasohibin prevents arterial neointimal formation through angiogenesis inhibition."
Yamashita H., Abe M., Watanabe K., Shimizu K., Moriya T., Sato A., Satomi S., Ohta H., Sonoda H., Sato Y.
Biochem. Biophys. Res. Commun. 345:919-925(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB028959 mRNA. Translation: BAA82988.2. Different initiation.
AL832588 mRNA. Translation: CAD89941.1.
AC007376 Genomic DNA. Translation: AAF02829.1.
AF111169 Genomic DNA. Translation: AAD44361.1. Sequence problems.
BC009031 mRNA. Translation: AAH09031.1.
BC051896 mRNA. Translation: AAH51896.1.
RefSeqNP_055724.1. NM_014909.4.
UniGeneHs.525479.

3D structure databases

ProteinModelPortalQ7L8A9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116518. 2 interactions.
STRING9606.ENSP00000167106.

PTM databases

PhosphoSiteQ7L8A9.

Polymorphism databases

DMDM62511163.

Proteomic databases

PaxDbQ7L8A9.
PRIDEQ7L8A9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000167106; ENSP00000167106; ENSG00000071246. [Q7L8A9-1]
ENST00000554237; ENSP00000451613; ENSG00000071246. [Q7L8A9-2]
GeneID22846.
KEGGhsa:22846.
UCSCuc001xss.3. human. [Q7L8A9-2]
uc001xst.2. human. [Q7L8A9-1]

Organism-specific databases

CTD22846.
GeneCardsGC14P077228.
HGNCHGNC:19964. VASH1.
HPAHPA000653.
MIM609011. gene.
neXtProtNX_Q7L8A9.
PharmGKBPA134941450.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG41266.
HOGENOMHOG000007477.
HOVERGENHBG079337.
InParanoidQ7L8A9.
OMAWERMWRH.
OrthoDBEOG7DC24N.
PhylomeDBQ7L8A9.
TreeFamTF329370.

Gene expression databases

ArrayExpressQ7L8A9.
BgeeQ7L8A9.
CleanExHS_VASH1.
GenevestigatorQ7L8A9.

Family and domain databases

InterProIPR028131. Vasohibin.
IPR028132. Vasohibin-1.
[Graphical view]
PANTHERPTHR32391. PTHR32391. 1 hit.
PTHR32391:SF1. PTHR32391:SF1. 1 hit.
PfamPF14822. Vasohibin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSVASH1. human.
GeneWikiVASH1.
GenomeRNAi22846.
NextBio43305.
PROQ7L8A9.
SOURCESearch...

Entry information

Entry nameVASH1_HUMAN
AccessionPrimary (citable) accession number: Q7L8A9
Secondary accession number(s): Q96H02, Q9UBF4, Q9Y629
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM