Q7L7V1 (DHX32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
EC=3.6.4.13

Alternative name(s):
DEAD/H box 32
DEAD/H helicase-like protein 1
Short name=DHLP1
DEAH box protein 32
HuDDX32

Gene names

Name:	DHX32
Synonyms:	DDX32

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus. Mitochondrion Ref.10.
Tissue specificity	Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes. Ref.1 Ref.9
Induction	Up-regulated by ionomycin in T-lymphocytes. Down-regulated in acute lymphoblastic leukemia. Ref.1 Ref.8
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence AAH37925.1 differs from that shown. Reason: Frameshift at position 439. The sequence BAB15029.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7L7V1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7L7V1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     284-364: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32		PRO_0000292663
Regions
	Domain	72 – 238	167	Helicase ATP-binding
	Nucleotide binding	85 – 92	8	ATP By similarity
	Motif	185 – 188	4	DEAH box
Natural variations
	Alternative sequence	284 – 364	81	Missing in isoform 2.		VSP_026427
	Natural variant	209	1	P → R in a breast cancer sample; somatic mutation. Ref.11		VAR_035843
	Natural variant	271	1	E → D. Corresponds to variant rs11244674 [ dbSNP | Ensembl ].		VAR_052181
	Natural variant	301	1	D → A. Corresponds to variant rs35772239 [ dbSNP | Ensembl ].		VAR_052182
	Natural variant	430	1	V → L. Corresponds to variant rs17153669 [ dbSNP | Ensembl ].		VAR_052183
Experimental info
	Sequence conflict	26	1	D → G in BAA91754. Ref.3
	Sequence conflict	123	1	V → M in BAA91882. Ref.3
	Sequence conflict	171	1	M → V in BAA91754. Ref.3
	Sequence conflict	231	1	N → F in AAL26551. Ref.1
	Sequence conflict	459	1	L → S in BAA91882. Ref.3
	Sequence conflict	496	1	A → G in BAA91882. Ref.3
	Sequence conflict	499	1	E → G in AAH37925. Ref.6
	Sequence conflict	567	1	V → A in BAB15029. Ref.3
	Sequence conflict	590	1	E → G in AAH37925. Ref.6
	Sequence conflict	686	1	P → L in BAA91882. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D6D5C570561C468A	FASTA	743	84,419
	10 20 30 40 50 60 MEEEGLECPN SSSEKRYFPE SLDSSDGDEE EVLACEDLEL NPFDGLPYSS RYYKLLKERE 70 80 90 100 110 120 DLPIWKEKYS FMENLLQNQI VIVSGDAKCG KSAQVPQWCA EYCLSIHYQH GGVICTQVHK 130 140 150 160 170 180 QTVVQLALRV ADEMDVNIGH EVGYVIPFEN CCTNETILRY CTDDMLQREM MSNPFLGSYG 190 200 210 220 230 240 VIILDDIHER SIATDVLLGL LKDVLLARPE LKLIINSSPH LISKLNSYYG NVPVIEVKNK 250 260 270 280 290 300 HPVEVVYLSE AQKDSFESIL RLIFEIHHSG EKGDIVVFLA CEQDIEKVCE TVYQGSNLNP 310 320 330 340 350 360 DLGELVVVPL YPKEKCSLFK PLDETEKRCQ VYQRRVVLTT SSGEFLIWSN SVRFVIDVGV 370 380 390 400 410 420 ERRKVYNPRI RANSLVMQPI SQSQAEIRKQ ILGSSSSGKF FCLYTEEFAS KDMTPLKPAE 430 440 450 460 470 480 MQEANLTSMV LFMKRIDIAG LGHCDFMNRP APESLMQALE DLDYLAALDN DGNLSEFGII 490 500 510 520 530 540 MSEFPLDPQL SKSILASCEF DCVDEVLTIA AMVTAPNCFS HVPHGAEEAA LTCWKTFLHP 550 560 570 580 590 600 EGDHFTLISI YKAYQDTTLN SSSEYCVEKW CRDYFLNCSA LRMADVIRAE LLEIIKRIEL 610 620 630 640 650 660 PYAEPAFGSK ENTLNIKKAL LSGYFMQIAR DVDGSGNYLM LTHKQVAQLH PLSGYSITKK 670 680 690 700 710 720 MPEWVLFHKF SISENNYIRI TSEISPELFM QLVPQYYFSN LPPSESKDIL QQVVDHLSPV 730 740 STMNKEQQMC ETCPETEQRC TLQ
	Isoform 2 [UniParc]. Checksum: 32A872984FC00460	FASTA	662	75,088
	10 20 30 40 50 60 MEEEGLECPN SSSEKRYFPE SLDSSDGDEE EVLACEDLEL NPFDGLPYSS RYYKLLKERE 70 80 90 100 110 120 DLPIWKEKYS FMENLLQNQI VIVSGDAKCG KSAQVPQWCA EYCLSIHYQH GGVICTQVHK 130 140 150 160 170 180 QTVVQLALRV ADEMDVNIGH EVGYVIPFEN CCTNETILRY CTDDMLQREM MSNPFLGSYG 190 200 210 220 230 240 VIILDDIHER SIATDVLLGL LKDVLLARPE LKLIINSSPH LISKLNSYYG NVPVIEVKNK 250 260 270 280 290 300 HPVEVVYLSE AQKDSFESIL RLIFEIHHSG EKGDIVVFLA CEQVYNPRIR ANSLVMQPIS 310 320 330 340 350 360 QSQAEIRKQI LGSSSSGKFF CLYTEEFASK DMTPLKPAEM QEANLTSMVL FMKRIDIAGL 370 380 390 400 410 420 GHCDFMNRPA PESLMQALED LDYLAALDND GNLSEFGIIM SEFPLDPQLS KSILASCEFD 430 440 450 460 470 480 CVDEVLTIAA MVTAPNCFSH VPHGAEEAAL TCWKTFLHPE GDHFTLISIY KAYQDTTLNS 490 500 510 520 530 540 SSEYCVEKWC RDYFLNCSAL RMADVIRAEL LEIIKRIELP YAEPAFGSKE NTLNIKKALL 550 560 570 580 590 600 SGYFMQIARD VDGSGNYLML THKQVAQLHP LSGYSITKKM PEWVLFHKFS ISENNYIRIT 610 620 630 640 650 660 SEISPELFMQ LVPQYYFSNL PPSESKDILQ QVVDHLSPVS TMNKEQQMCE TCPETEQRCT LQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The novel helicase homologue DDX32 is down-regulated in acute lymphoblastic leukemia." Abdelhaleem M. Leuk. Res. 26:945-954(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 230-414 (ISOFORM 2), INDUCTION, TISSUE SPECIFICITY. Tissue: Myeloid leukemia cell.
[2]	"Genomic structure of the human BCCIP gene and its expression in cancer." Meng X., Liu J., Shen Z. Gene 302:139-146(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon and Teratocarcinoma.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain, Kidney and Placenta.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-743 (ISOFORM 1). Tissue: Testis.
[8]	"The activation-induced expression of DHX32 in Jurkat T cells is specific and involves calcium and nuclear factor of activated T cells." Alli Z., Nam E.H., Beimnet K., Abdelhaleem M. Cell. Immunol. 237:141-146(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[9]	"Expression of DHX32 in lymphoid tissues." Alli Z., Ho M., Abdelhaleem M. Exp. Mol. Pathol. 79:219-223(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"Nuclear and mitochondrial localization of the putative RNA helicase DHX32." Alli Z., Ackerley C., Chen Y., Al-Saud B., Abdelhaleem M. Exp. Mol. Pathol. 81:245-248(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[11]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-209.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF427340 mRNA. Translation: AAL26550.1. AF427341 mRNA. Translation: AAL26551.1. AY064247 Genomic DNA. Translation: AAL55437.1. AY064250 mRNA. Translation: AAL55441.1. AK001556 mRNA. Translation: BAA91754.1. AK001751 mRNA. Translation: BAA91882.1. AK024869 mRNA. Translation: BAB15029.1. Different initiation. AL360176 Genomic DNA. Translation: CAI12094.1. AL360176 Genomic DNA. Translation: CAI12095.1. AL360176 Genomic DNA. Translation: CAI12096.1. CH471066 Genomic DNA. Translation: EAW49216.1. CH471066 Genomic DNA. Translation: EAW49217.1. BC002473 mRNA. Translation: AAH02473.3. BC037925 mRNA. Translation: AAH37925.1. Frameshift. BC068471 mRNA. Translation: AAH68471.1. AL162051 mRNA. Translation: CAB82394.1.
IPI	IPI00334601. IPI00644447.
PIR	T47184.
RefSeq	NP_060650.2. NM_018180.2.
UniGene	Hs.370292.
3D structure databases
ProteinModelPortal	Q7L7V1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7L7V1. 1 interaction.
PTM databases
PhosphoSite	Q7L7V1.
Polymorphism databases
DMDM	74759011.
Proteomic databases
PaxDb	Q7L7V1.
PRIDE	Q7L7V1.
Protocols and materials databases
DNASU	55760.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000284688; ENSP00000284688; ENSG00000089876. ENST00000284690; ENSP00000284690; ENSG00000089876. ENST00000368721; ENSP00000357710; ENSG00000089876. ENST00000415732; ENSP00000406781; ENSG00000089876.
GeneID	55760.
KEGG	hsa:55760.
UCSC	uc001lje.1. human. uc001ljf.1. human.
Organism-specific databases
CTD	55760.
GeneCards	GC10M127515.
HGNC	HGNC:16717. DHX32.
HPA	HPA048872.
MIM	607960. gene.
neXtProt	NX_Q7L7V1.
PharmGKB	PA27219.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1643.
HOVERGEN	HBG039428.
InParanoid	Q7L7V1.
OMA	LACEQDI.
OrthoDB	EOG4N30NF.
PhylomeDB	Q7L7V1.
Gene expression databases
Bgee	Q7L7V1.
Genevestigator	Q7L7V1.
Family and domain databases
InterPro	IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR014001. Helicase_ATP-bd. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF04408. HA2. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view]
SMART	SM00847. HA2. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DHX32. human.
GenomeRNAi	55760.
NextBio	60779.
SOURCE	Search...

Entry information

Entry name

DHX32_HUMAN

Accession

Primary (citable) accession number: Q7L7V1
Secondary accession number(s): A8MSV2 , D3DRF9, Q49AG5, Q5T3L0, Q5T3L5, Q96NY1, Q9BUN0, Q9H769, Q9NSL5, Q9NV74, Q9NVJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.