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Protein

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Gene

DHX32

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 (EC:3.6.4.13)
Alternative name(s):
DEAD/H box 32
DEAD/H helicase-like protein 1
Short name:
DHLP1
DEAH box protein 32
HuDDX32
Gene namesi
Name:DHX32
Synonyms:DDX32
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:16717. DHX32.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55760.
OpenTargetsiENSG00000089876.
PharmGKBiPA27219.

Polymorphism and mutation databases

BioMutaiDHX32.
DMDMi74759011.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002926631 – 743Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32Add BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ7L7V1.
MaxQBiQ7L7V1.
PaxDbiQ7L7V1.
PeptideAtlasiQ7L7V1.
PRIDEiQ7L7V1.

PTM databases

iPTMnetiQ7L7V1.
PhosphoSitePlusiQ7L7V1.

Expressioni

Tissue specificityi

Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes.2 Publications

Inductioni

Up-regulated by ionomycin in T-lymphocytes. Down-regulated in acute lymphoblastic leukemia.2 Publications

Gene expression databases

BgeeiENSG00000089876.
ExpressionAtlasiQ7L7V1. baseline and differential.
GenevisibleiQ7L7V1. HS.

Organism-specific databases

HPAiHPA048872.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
FAM161AQ3B8207EBI-2807297,EBI-719941

Protein-protein interaction databases

BioGridi120878. 8 interactors.
IntActiQ7L7V1. 2 interactors.
STRINGi9606.ENSP00000284690.

Structurei

3D structure databases

ProteinModelPortaliQ7L7V1.
SMRiQ7L7V1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 238Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST167

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi185 – 188DEAH box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00860000133783.
HOVERGENiHBG039428.
InParanoidiQ7L7V1.
KOiK18994.
OMAiSGDAKCG.
OrthoDBiEOG091G025L.
PhylomeDBiQ7L7V1.
TreeFamiTF105735.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00847. HA2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7L7V1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEEGLECPN SSSEKRYFPE SLDSSDGDEE EVLACEDLEL NPFDGLPYSS
60 70 80 90 100
RYYKLLKERE DLPIWKEKYS FMENLLQNQI VIVSGDAKCG KSAQVPQWCA
110 120 130 140 150
EYCLSIHYQH GGVICTQVHK QTVVQLALRV ADEMDVNIGH EVGYVIPFEN
160 170 180 190 200
CCTNETILRY CTDDMLQREM MSNPFLGSYG VIILDDIHER SIATDVLLGL
210 220 230 240 250
LKDVLLARPE LKLIINSSPH LISKLNSYYG NVPVIEVKNK HPVEVVYLSE
260 270 280 290 300
AQKDSFESIL RLIFEIHHSG EKGDIVVFLA CEQDIEKVCE TVYQGSNLNP
310 320 330 340 350
DLGELVVVPL YPKEKCSLFK PLDETEKRCQ VYQRRVVLTT SSGEFLIWSN
360 370 380 390 400
SVRFVIDVGV ERRKVYNPRI RANSLVMQPI SQSQAEIRKQ ILGSSSSGKF
410 420 430 440 450
FCLYTEEFAS KDMTPLKPAE MQEANLTSMV LFMKRIDIAG LGHCDFMNRP
460 470 480 490 500
APESLMQALE DLDYLAALDN DGNLSEFGII MSEFPLDPQL SKSILASCEF
510 520 530 540 550
DCVDEVLTIA AMVTAPNCFS HVPHGAEEAA LTCWKTFLHP EGDHFTLISI
560 570 580 590 600
YKAYQDTTLN SSSEYCVEKW CRDYFLNCSA LRMADVIRAE LLEIIKRIEL
610 620 630 640 650
PYAEPAFGSK ENTLNIKKAL LSGYFMQIAR DVDGSGNYLM LTHKQVAQLH
660 670 680 690 700
PLSGYSITKK MPEWVLFHKF SISENNYIRI TSEISPELFM QLVPQYYFSN
710 720 730 740
LPPSESKDIL QQVVDHLSPV STMNKEQQMC ETCPETEQRC TLQ
Length:743
Mass (Da):84,419
Last modified:July 5, 2004 - v1
Checksum:iD6D5C570561C468A
GO
Isoform 2 (identifier: Q7L7V1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-364: Missing.

Show »
Length:662
Mass (Da):75,088
Checksum:i32A872984FC00460
GO

Sequence cautioni

The sequence AAH37925 differs from that shown. Reason: Frameshift at position 439.Curated
The sequence BAB15029 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26D → G in BAA91754 (PubMed:14702039).Curated1
Sequence conflicti123V → M in BAA91882 (PubMed:14702039).Curated1
Sequence conflicti171M → V in BAA91754 (PubMed:14702039).Curated1
Sequence conflicti231N → F in AAL26551 (PubMed:12163057).Curated1
Sequence conflicti459L → S in BAA91882 (PubMed:14702039).Curated1
Sequence conflicti496A → G in BAA91882 (PubMed:14702039).Curated1
Sequence conflicti499E → G in AAH37925 (PubMed:15489334).Curated1
Sequence conflicti567V → A in BAB15029 (PubMed:14702039).Curated1
Sequence conflicti590E → G in AAH37925 (PubMed:15489334).Curated1
Sequence conflicti686P → L in BAA91882 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035843209P → R in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_052181271E → D.Corresponds to variant rs11244674dbSNPEnsembl.1
Natural variantiVAR_052182301D → A.Corresponds to variant rs35772239dbSNPEnsembl.1
Natural variantiVAR_052183430V → L.Corresponds to variant rs17153669dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026427284 – 364Missing in isoform 2. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF427340 mRNA. Translation: AAL26550.1.
AF427341 mRNA. Translation: AAL26551.1.
AY064247 Genomic DNA. Translation: AAL55437.1.
AY064250 mRNA. Translation: AAL55441.1.
AK001556 mRNA. Translation: BAA91754.1.
AK001751 mRNA. Translation: BAA91882.1.
AK024869 mRNA. Translation: BAB15029.1. Different initiation.
AL360176 Genomic DNA. Translation: CAI12094.1.
AL360176 Genomic DNA. Translation: CAI12095.1.
AL360176 Genomic DNA. Translation: CAI12096.1.
CH471066 Genomic DNA. Translation: EAW49216.1.
CH471066 Genomic DNA. Translation: EAW49217.1.
BC002473 mRNA. Translation: AAH02473.3.
BC037925 mRNA. Translation: AAH37925.1. Frameshift.
BC068471 mRNA. Translation: AAH68471.1.
AL162051 mRNA. Translation: CAB82394.1.
CCDSiCCDS7652.1. [Q7L7V1-1]
PIRiT47184.
RefSeqiNP_060650.2. NM_018180.2. [Q7L7V1-1]
XP_016871893.1. XM_017016404.1. [Q7L7V1-1]
XP_016871894.1. XM_017016405.1. [Q7L7V1-1]
UniGeneiHs.370292.

Genome annotation databases

EnsembliENST00000284690; ENSP00000284690; ENSG00000089876. [Q7L7V1-1]
GeneIDi55760.
KEGGihsa:55760.
UCSCiuc001ljf.1. human. [Q7L7V1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF427340 mRNA. Translation: AAL26550.1.
AF427341 mRNA. Translation: AAL26551.1.
AY064247 Genomic DNA. Translation: AAL55437.1.
AY064250 mRNA. Translation: AAL55441.1.
AK001556 mRNA. Translation: BAA91754.1.
AK001751 mRNA. Translation: BAA91882.1.
AK024869 mRNA. Translation: BAB15029.1. Different initiation.
AL360176 Genomic DNA. Translation: CAI12094.1.
AL360176 Genomic DNA. Translation: CAI12095.1.
AL360176 Genomic DNA. Translation: CAI12096.1.
CH471066 Genomic DNA. Translation: EAW49216.1.
CH471066 Genomic DNA. Translation: EAW49217.1.
BC002473 mRNA. Translation: AAH02473.3.
BC037925 mRNA. Translation: AAH37925.1. Frameshift.
BC068471 mRNA. Translation: AAH68471.1.
AL162051 mRNA. Translation: CAB82394.1.
CCDSiCCDS7652.1. [Q7L7V1-1]
PIRiT47184.
RefSeqiNP_060650.2. NM_018180.2. [Q7L7V1-1]
XP_016871893.1. XM_017016404.1. [Q7L7V1-1]
XP_016871894.1. XM_017016405.1. [Q7L7V1-1]
UniGeneiHs.370292.

3D structure databases

ProteinModelPortaliQ7L7V1.
SMRiQ7L7V1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120878. 8 interactors.
IntActiQ7L7V1. 2 interactors.
STRINGi9606.ENSP00000284690.

PTM databases

iPTMnetiQ7L7V1.
PhosphoSitePlusiQ7L7V1.

Polymorphism and mutation databases

BioMutaiDHX32.
DMDMi74759011.

Proteomic databases

EPDiQ7L7V1.
MaxQBiQ7L7V1.
PaxDbiQ7L7V1.
PeptideAtlasiQ7L7V1.
PRIDEiQ7L7V1.

Protocols and materials databases

DNASUi55760.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284690; ENSP00000284690; ENSG00000089876. [Q7L7V1-1]
GeneIDi55760.
KEGGihsa:55760.
UCSCiuc001ljf.1. human. [Q7L7V1-1]

Organism-specific databases

CTDi55760.
DisGeNETi55760.
GeneCardsiDHX32.
HGNCiHGNC:16717. DHX32.
HPAiHPA048872.
MIMi607960. gene.
neXtProtiNX_Q7L7V1.
OpenTargetsiENSG00000089876.
PharmGKBiPA27219.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00860000133783.
HOVERGENiHBG039428.
InParanoidiQ7L7V1.
KOiK18994.
OMAiSGDAKCG.
OrthoDBiEOG091G025L.
PhylomeDBiQ7L7V1.
TreeFamiTF105735.

Miscellaneous databases

ChiTaRSiDHX32. human.
GeneWikiiDHX32.
GenomeRNAii55760.
PROiQ7L7V1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000089876.
ExpressionAtlasiQ7L7V1. baseline and differential.
GenevisibleiQ7L7V1. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00847. HA2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHX32_HUMAN
AccessioniPrimary (citable) accession number: Q7L7V1
Secondary accession number(s): A8MSV2
, D3DRF9, Q49AG5, Q5T3L0, Q5T3L5, Q96NY1, Q9BUN0, Q9H769, Q9NSL5, Q9NV74, Q9NVJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.