Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7L7V1 (DHX32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

EC=3.6.4.13
Alternative name(s):
DEAD/H box 32
DEAD/H helicase-like protein 1
Short name=DHLP1
DEAH box protein 32
HuDDX32
Gene names
Name:DHX32
Synonyms:DDX32
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length743 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Nucleus. Mitochondrion Ref.10.

Tissue specificity

Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes. Ref.1 Ref.9

Induction

Up-regulated by ionomycin in T-lymphocytes. Down-regulated in acute lymphoblastic leukemia. Ref.1 Ref.8

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence AAH37925.1 differs from that shown. Reason: Frameshift at position 439.

The sequence BAB15029.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentMitochondrion
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandATP-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7L7V1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7L7V1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     284-364: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 743743Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
PRO_0000292663

Regions

Domain72 – 238167Helicase ATP-binding
Nucleotide binding85 – 928ATP By similarity
Motif185 – 1884DEAH box

Amino acid modifications

Modified residue11N-acetylmethionine Ref.11

Natural variations

Alternative sequence284 – 36481Missing in isoform 2.
VSP_026427
Natural variant2091P → R in a breast cancer sample; somatic mutation. Ref.12
VAR_035843
Natural variant2711E → D.
Corresponds to variant rs11244674 [ dbSNP | Ensembl ].
VAR_052181
Natural variant3011D → A.
Corresponds to variant rs35772239 [ dbSNP | Ensembl ].
VAR_052182
Natural variant4301V → L.
Corresponds to variant rs17153669 [ dbSNP | Ensembl ].
VAR_052183

Experimental info

Sequence conflict261D → G in BAA91754. Ref.3
Sequence conflict1231V → M in BAA91882. Ref.3
Sequence conflict1711M → V in BAA91754. Ref.3
Sequence conflict2311N → F in AAL26551. Ref.1
Sequence conflict4591L → S in BAA91882. Ref.3
Sequence conflict4961A → G in BAA91882. Ref.3
Sequence conflict4991E → G in AAH37925. Ref.6
Sequence conflict5671V → A in BAB15029. Ref.3
Sequence conflict5901E → G in AAH37925. Ref.6
Sequence conflict6861P → L in BAA91882. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: D6D5C570561C468A

FASTA74384,419
        10         20         30         40         50         60 
MEEEGLECPN SSSEKRYFPE SLDSSDGDEE EVLACEDLEL NPFDGLPYSS RYYKLLKERE 

        70         80         90        100        110        120 
DLPIWKEKYS FMENLLQNQI VIVSGDAKCG KSAQVPQWCA EYCLSIHYQH GGVICTQVHK 

       130        140        150        160        170        180 
QTVVQLALRV ADEMDVNIGH EVGYVIPFEN CCTNETILRY CTDDMLQREM MSNPFLGSYG 

       190        200        210        220        230        240 
VIILDDIHER SIATDVLLGL LKDVLLARPE LKLIINSSPH LISKLNSYYG NVPVIEVKNK 

       250        260        270        280        290        300 
HPVEVVYLSE AQKDSFESIL RLIFEIHHSG EKGDIVVFLA CEQDIEKVCE TVYQGSNLNP 

       310        320        330        340        350        360 
DLGELVVVPL YPKEKCSLFK PLDETEKRCQ VYQRRVVLTT SSGEFLIWSN SVRFVIDVGV 

       370        380        390        400        410        420 
ERRKVYNPRI RANSLVMQPI SQSQAEIRKQ ILGSSSSGKF FCLYTEEFAS KDMTPLKPAE 

       430        440        450        460        470        480 
MQEANLTSMV LFMKRIDIAG LGHCDFMNRP APESLMQALE DLDYLAALDN DGNLSEFGII 

       490        500        510        520        530        540 
MSEFPLDPQL SKSILASCEF DCVDEVLTIA AMVTAPNCFS HVPHGAEEAA LTCWKTFLHP 

       550        560        570        580        590        600 
EGDHFTLISI YKAYQDTTLN SSSEYCVEKW CRDYFLNCSA LRMADVIRAE LLEIIKRIEL 

       610        620        630        640        650        660 
PYAEPAFGSK ENTLNIKKAL LSGYFMQIAR DVDGSGNYLM LTHKQVAQLH PLSGYSITKK 

       670        680        690        700        710        720 
MPEWVLFHKF SISENNYIRI TSEISPELFM QLVPQYYFSN LPPSESKDIL QQVVDHLSPV 

       730        740 
STMNKEQQMC ETCPETEQRC TLQ 

« Hide

Isoform 2 [UniParc].

Checksum: 32A872984FC00460
Show »

FASTA66275,088

References

« Hide 'large scale' references
[1]"The novel helicase homologue DDX32 is down-regulated in acute lymphoblastic leukemia."
Abdelhaleem M.
Leuk. Res. 26:945-954(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 230-414 (ISOFORM 2), INDUCTION, TISSUE SPECIFICITY.
Tissue: Myeloid leukemia cell.
[2]"Genomic structure of the human BCCIP gene and its expression in cancer."
Meng X., Liu J., Shen Z.
Gene 302:139-146(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Colon and Teratocarcinoma.
[4]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain, Kidney and Placenta.
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-743 (ISOFORM 1).
Tissue: Testis.
[8]"The activation-induced expression of DHX32 in Jurkat T cells is specific and involves calcium and nuclear factor of activated T cells."
Alli Z., Nam E.H., Beimnet K., Abdelhaleem M.
Cell. Immunol. 237:141-146(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[9]"Expression of DHX32 in lymphoid tissues."
Alli Z., Ho M., Abdelhaleem M.
Exp. Mol. Pathol. 79:219-223(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Nuclear and mitochondrial localization of the putative RNA helicase DHX32."
Alli Z., Ackerley C., Chen Y., Al-Saud B., Abdelhaleem M.
Exp. Mol. Pathol. 81:245-248(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-209.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF427340 mRNA. Translation: AAL26550.1.
AF427341 mRNA. Translation: AAL26551.1.
AY064247 Genomic DNA. Translation: AAL55437.1.
AY064250 mRNA. Translation: AAL55441.1.
AK001556 mRNA. Translation: BAA91754.1.
AK001751 mRNA. Translation: BAA91882.1.
AK024869 mRNA. Translation: BAB15029.1. Different initiation.
AL360176 Genomic DNA. Translation: CAI12094.1.
AL360176 Genomic DNA. Translation: CAI12095.1.
AL360176 Genomic DNA. Translation: CAI12096.1.
CH471066 Genomic DNA. Translation: EAW49216.1.
CH471066 Genomic DNA. Translation: EAW49217.1.
BC002473 mRNA. Translation: AAH02473.3.
BC037925 mRNA. Translation: AAH37925.1. Frameshift.
BC068471 mRNA. Translation: AAH68471.1.
AL162051 mRNA. Translation: CAB82394.1.
PIRT47184.
RefSeqNP_060650.2. NM_018180.2.
UniGeneHs.370292.

3D structure databases

ProteinModelPortalQ7L7V1.
SMRQ7L7V1. Positions 30-713.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120878. 1 interaction.
IntActQ7L7V1. 1 interaction.

PTM databases

PhosphoSiteQ7L7V1.

Polymorphism databases

DMDM74759011.

Proteomic databases

PaxDbQ7L7V1.
PRIDEQ7L7V1.

Protocols and materials databases

DNASU55760.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000284688; ENSP00000284688; ENSG00000089876. [Q7L7V1-2]
ENST00000284690; ENSP00000284690; ENSG00000089876. [Q7L7V1-1]
GeneID55760.
KEGGhsa:55760.
UCSCuc001lje.1. human. [Q7L7V1-2]
uc001ljf.1. human. [Q7L7V1-1]

Organism-specific databases

CTD55760.
GeneCardsGC10M127515.
HGNCHGNC:16717. DHX32.
HPAHPA048872.
MIM607960. gene.
neXtProtNX_Q7L7V1.
PharmGKBPA27219.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1643.
HOVERGENHBG039428.
InParanoidQ7L7V1.
OMALACEQDI.
OrthoDBEOG715Q3J.
PhylomeDBQ7L7V1.
TreeFamTF105735.

Gene expression databases

BgeeQ7L7V1.
GenevestigatorQ7L7V1.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF04408. HA2. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTSM00847. HA2. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDHX32. human.
GeneWikiDHX32.
GenomeRNAi55760.
NextBio60779.
PROQ7L7V1.
SOURCESearch...

Entry information

Entry nameDHX32_HUMAN
AccessionPrimary (citable) accession number: Q7L7V1
Secondary accession number(s): A8MSV2 expand/collapse secondary AC list , D3DRF9, Q49AG5, Q5T3L0, Q5T3L5, Q96NY1, Q9BUN0, Q9H769, Q9NSL5, Q9NV74, Q9NVJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM