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Q7L7V1

- DHX32_HUMAN

UniProt

Q7L7V1 - DHX32_HUMAN

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Protein
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
Gene
DHX32, DDX32
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 928ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 (EC:3.6.4.13)
Alternative name(s):
DEAD/H box 32
DEAD/H helicase-like protein 1
Short name:
DHLP1
DEAH box protein 32
HuDDX32
Gene namesi
Name:DHX32
Synonyms:DDX32
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:16717. DHX32.

Subcellular locationi

Nucleus. Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27219.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 743743Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
PRO_0000292663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ7L7V1.
PaxDbiQ7L7V1.
PRIDEiQ7L7V1.

PTM databases

PhosphoSiteiQ7L7V1.

Expressioni

Tissue specificityi

Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes.2 Publications

Inductioni

Up-regulated by ionomycin in T-lymphocytes. Down-regulated in acute lymphoblastic leukemia.2 Publications

Gene expression databases

BgeeiQ7L7V1.
GenevestigatoriQ7L7V1.

Organism-specific databases

HPAiHPA048872.

Interactioni

Protein-protein interaction databases

BioGridi120878. 1 interaction.
IntActiQ7L7V1. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ7L7V1.
SMRiQ7L7V1. Positions 30-713.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 238167Helicase ATP-binding
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi185 – 1884DEAH box

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1643.
HOVERGENiHBG039428.
InParanoidiQ7L7V1.
OMAiLACEQDI.
OrthoDBiEOG715Q3J.
PhylomeDBiQ7L7V1.
TreeFamiTF105735.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00847. HA2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7L7V1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEEEGLECPN SSSEKRYFPE SLDSSDGDEE EVLACEDLEL NPFDGLPYSS    50
RYYKLLKERE DLPIWKEKYS FMENLLQNQI VIVSGDAKCG KSAQVPQWCA 100
EYCLSIHYQH GGVICTQVHK QTVVQLALRV ADEMDVNIGH EVGYVIPFEN 150
CCTNETILRY CTDDMLQREM MSNPFLGSYG VIILDDIHER SIATDVLLGL 200
LKDVLLARPE LKLIINSSPH LISKLNSYYG NVPVIEVKNK HPVEVVYLSE 250
AQKDSFESIL RLIFEIHHSG EKGDIVVFLA CEQDIEKVCE TVYQGSNLNP 300
DLGELVVVPL YPKEKCSLFK PLDETEKRCQ VYQRRVVLTT SSGEFLIWSN 350
SVRFVIDVGV ERRKVYNPRI RANSLVMQPI SQSQAEIRKQ ILGSSSSGKF 400
FCLYTEEFAS KDMTPLKPAE MQEANLTSMV LFMKRIDIAG LGHCDFMNRP 450
APESLMQALE DLDYLAALDN DGNLSEFGII MSEFPLDPQL SKSILASCEF 500
DCVDEVLTIA AMVTAPNCFS HVPHGAEEAA LTCWKTFLHP EGDHFTLISI 550
YKAYQDTTLN SSSEYCVEKW CRDYFLNCSA LRMADVIRAE LLEIIKRIEL 600
PYAEPAFGSK ENTLNIKKAL LSGYFMQIAR DVDGSGNYLM LTHKQVAQLH 650
PLSGYSITKK MPEWVLFHKF SISENNYIRI TSEISPELFM QLVPQYYFSN 700
LPPSESKDIL QQVVDHLSPV STMNKEQQMC ETCPETEQRC TLQ 743
Length:743
Mass (Da):84,419
Last modified:July 5, 2004 - v1
Checksum:iD6D5C570561C468A
GO
Isoform 2 (identifier: Q7L7V1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-364: Missing.

Show »
Length:662
Mass (Da):75,088
Checksum:i32A872984FC00460
GO

Sequence cautioni

The sequence AAH37925.1 differs from that shown. Reason: Frameshift at position 439.
The sequence BAB15029.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti209 – 2091P → R in a breast cancer sample; somatic mutation. 1 Publication
VAR_035843
Natural varianti271 – 2711E → D.
Corresponds to variant rs11244674 [ dbSNP | Ensembl ].
VAR_052181
Natural varianti301 – 3011D → A.
Corresponds to variant rs35772239 [ dbSNP | Ensembl ].
VAR_052182
Natural varianti430 – 4301V → L.
Corresponds to variant rs17153669 [ dbSNP | Ensembl ].
VAR_052183

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei284 – 36481Missing in isoform 2.
VSP_026427Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261D → G in BAA91754. 1 Publication
Sequence conflicti123 – 1231V → M in BAA91882. 1 Publication
Sequence conflicti171 – 1711M → V in BAA91754. 1 Publication
Sequence conflicti231 – 2311N → F in AAL26551. 1 Publication
Sequence conflicti459 – 4591L → S in BAA91882. 1 Publication
Sequence conflicti496 – 4961A → G in BAA91882. 1 Publication
Sequence conflicti499 – 4991E → G in AAH37925. 1 Publication
Sequence conflicti567 – 5671V → A in BAB15029. 1 Publication
Sequence conflicti590 – 5901E → G in AAH37925. 1 Publication
Sequence conflicti686 – 6861P → L in BAA91882. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF427340 mRNA. Translation: AAL26550.1.
AF427341 mRNA. Translation: AAL26551.1.
AY064247 Genomic DNA. Translation: AAL55437.1.
AY064250 mRNA. Translation: AAL55441.1.
AK001556 mRNA. Translation: BAA91754.1.
AK001751 mRNA. Translation: BAA91882.1.
AK024869 mRNA. Translation: BAB15029.1. Different initiation.
AL360176 Genomic DNA. Translation: CAI12094.1.
AL360176 Genomic DNA. Translation: CAI12095.1.
AL360176 Genomic DNA. Translation: CAI12096.1.
CH471066 Genomic DNA. Translation: EAW49216.1.
CH471066 Genomic DNA. Translation: EAW49217.1.
BC002473 mRNA. Translation: AAH02473.3.
BC037925 mRNA. Translation: AAH37925.1. Frameshift.
BC068471 mRNA. Translation: AAH68471.1.
AL162051 mRNA. Translation: CAB82394.1.
CCDSiCCDS7652.1. [Q7L7V1-1]
PIRiT47184.
RefSeqiNP_060650.2. NM_018180.2. [Q7L7V1-1]
UniGeneiHs.370292.

Genome annotation databases

EnsembliENST00000284688; ENSP00000284688; ENSG00000089876. [Q7L7V1-2]
ENST00000284690; ENSP00000284690; ENSG00000089876. [Q7L7V1-1]
GeneIDi55760.
KEGGihsa:55760.
UCSCiuc001lje.1. human. [Q7L7V1-2]
uc001ljf.1. human. [Q7L7V1-1]

Polymorphism databases

DMDMi74759011.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF427340 mRNA. Translation: AAL26550.1 .
AF427341 mRNA. Translation: AAL26551.1 .
AY064247 Genomic DNA. Translation: AAL55437.1 .
AY064250 mRNA. Translation: AAL55441.1 .
AK001556 mRNA. Translation: BAA91754.1 .
AK001751 mRNA. Translation: BAA91882.1 .
AK024869 mRNA. Translation: BAB15029.1 . Different initiation.
AL360176 Genomic DNA. Translation: CAI12094.1 .
AL360176 Genomic DNA. Translation: CAI12095.1 .
AL360176 Genomic DNA. Translation: CAI12096.1 .
CH471066 Genomic DNA. Translation: EAW49216.1 .
CH471066 Genomic DNA. Translation: EAW49217.1 .
BC002473 mRNA. Translation: AAH02473.3 .
BC037925 mRNA. Translation: AAH37925.1 . Frameshift.
BC068471 mRNA. Translation: AAH68471.1 .
AL162051 mRNA. Translation: CAB82394.1 .
CCDSi CCDS7652.1. [Q7L7V1-1 ]
PIRi T47184.
RefSeqi NP_060650.2. NM_018180.2. [Q7L7V1-1 ]
UniGenei Hs.370292.

3D structure databases

ProteinModelPortali Q7L7V1.
SMRi Q7L7V1. Positions 30-713.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120878. 1 interaction.
IntActi Q7L7V1. 1 interaction.

PTM databases

PhosphoSitei Q7L7V1.

Polymorphism databases

DMDMi 74759011.

Proteomic databases

MaxQBi Q7L7V1.
PaxDbi Q7L7V1.
PRIDEi Q7L7V1.

Protocols and materials databases

DNASUi 55760.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000284688 ; ENSP00000284688 ; ENSG00000089876 . [Q7L7V1-2 ]
ENST00000284690 ; ENSP00000284690 ; ENSG00000089876 . [Q7L7V1-1 ]
GeneIDi 55760.
KEGGi hsa:55760.
UCSCi uc001lje.1. human. [Q7L7V1-2 ]
uc001ljf.1. human. [Q7L7V1-1 ]

Organism-specific databases

CTDi 55760.
GeneCardsi GC10M127515.
HGNCi HGNC:16717. DHX32.
HPAi HPA048872.
MIMi 607960. gene.
neXtProti NX_Q7L7V1.
PharmGKBi PA27219.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1643.
HOVERGENi HBG039428.
InParanoidi Q7L7V1.
OMAi LACEQDI.
OrthoDBi EOG715Q3J.
PhylomeDBi Q7L7V1.
TreeFami TF105735.

Miscellaneous databases

ChiTaRSi DHX32. human.
GeneWikii DHX32.
GenomeRNAii 55760.
NextBioi 60779.
PROi Q7L7V1.
SOURCEi Search...

Gene expression databases

Bgeei Q7L7V1.
Genevestigatori Q7L7V1.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF04408. HA2. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view ]
SMARTi SM00847. HA2. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The novel helicase homologue DDX32 is down-regulated in acute lymphoblastic leukemia."
    Abdelhaleem M.
    Leuk. Res. 26:945-954(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 230-414 (ISOFORM 2), INDUCTION, TISSUE SPECIFICITY.
    Tissue: Myeloid leukemia cell.
  2. "Genomic structure of the human BCCIP gene and its expression in cancer."
    Meng X., Liu J., Shen Z.
    Gene 302:139-146(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon and Teratocarcinoma.
  4. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain, Kidney and Placenta.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 238-743 (ISOFORM 1).
    Tissue: Testis.
  8. "The activation-induced expression of DHX32 in Jurkat T cells is specific and involves calcium and nuclear factor of activated T cells."
    Alli Z., Nam E.H., Beimnet K., Abdelhaleem M.
    Cell. Immunol. 237:141-146(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. Cited for: TISSUE SPECIFICITY.
  10. "Nuclear and mitochondrial localization of the putative RNA helicase DHX32."
    Alli Z., Ackerley C., Chen Y., Al-Saud B., Abdelhaleem M.
    Exp. Mol. Pathol. 81:245-248(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-209.

Entry informationi

Entry nameiDHX32_HUMAN
AccessioniPrimary (citable) accession number: Q7L7V1
Secondary accession number(s): A8MSV2
, D3DRF9, Q49AG5, Q5T3L0, Q5T3L5, Q96NY1, Q9BUN0, Q9H769, Q9NSL5, Q9NV74, Q9NVJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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