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Q7L622 (G2E3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G2/M phase-specific E3 ubiquitin-protein ligase

EC=6.3.2.-
Gene names
Name:G2E3
Synonyms:KIAA1333
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death. Ref.6

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Nucleusnucleolus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm. In the nucleus, delocalizes from the nucleolus to the nucleoplasm in response to DNA damage. Ref.1

Tissue specificity

Predominantly expressed in brain, liver, kidney, testes and ovary. Ref.2

Induction

Up-regulated approximately 4-fold in G2 when compared to S phase. Down-regulated approximately 3-fold by gamma-irradiation. Ref.5

Domain

Ubiquitin ligase activity is mediated by two distinct domains, PHD-type zinc fingers 2 and 3. The use of these distinct domains may allow ubiquitination of different targets by each domain. The HECT domain is catalytically inactive and does not contribute to this activity. Ref.6

Sequence similarities

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Contains 3 PHD-type zinc fingers.

Sequence caution

The sequence BAA92571.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB14280.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706G2/M phase-specific E3 ubiquitin-protein ligase
PRO_0000248343

Regions

Domain371 – 698328HECT
Zinc finger80 – 12849PHD-type 1
Zinc finger143 – 19351PHD-type 2; degenerate
Zinc finger237 – 28650PHD-type 3

Natural variations

Natural variant2321R → H.
Corresponds to variant rs17096934 [ dbSNP | Ensembl ].
VAR_027273

Experimental info

Mutagenesis30 – 312KK → AA: Loss of nucleolar localization. No effect on nuclear localization.
Mutagenesis841C → A: Strong activity; when associated with A-258; A-261 and A-666. Strong activity; when associated with A-147 and A-666. No activity; when associated with A-147; A-258 and A-261. Ref.6
Mutagenesis1471C → A: Strong activity; when associated with A-84 and A-666. No activity; when associated with A-258; A-261 and A-666. No activity; when associated with A-84; A-258 and A-261. Ref.6
Mutagenesis2581C → A: Strong activity; when associated with A-84; A-261 and A-666. No activity; when associated with A-147; A-261 and A-666. No activity; when associated with A-84; A-147 and A-261. Ref.6
Mutagenesis2611C → A: Strong activity; when associated with A-84; A-258 and A-666. No activity; when associated with A-84; A-147 and A-258. No activity; when associated with A-147; A-258 and A-666. Ref.6
Mutagenesis6661C → A: No effect on subcellular location. Strong activity; when associated with A-84; A-258 and A261. Strong activity; when associated with A-84 and A-147. No activity; when associated with A-147; A-258 and A-261. Ref.1 Ref.6
Sequence conflict1841V → A in BAA91095. Ref.3
Sequence conflict6271T → A in BAB14280. Ref.3
Sequence conflict6651K → E in BAA91095. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q7L622 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 4B46ACF8782F941A

FASTA70680,504
        10         20         30         40         50         60 
MNESKPGDSQ NLACVFCRKH DDCPNKYGEK KTKEKWNLTV HYYCLLMSSG IWQRGKEEEG 

        70         80         90        100        110        120 
VYGFLIEDIR KEVNRASKLK CCVCKKNGAS IGCVAPRCKR SYHFPCGLQR ECIFQFTGNF 

       130        140        150        160        170        180 
ASFCWDHRPV QIITSNNYRE SLPCTICLEF IEPIPSYNIL RSPCCKNAWF HRDCLQVQAI 

       190        200        210        220        230        240 
NAGVFFFRCT ICNNSDIFQK EMLRMGIHIP EKDASWELEE NAYQELLQHY ERCDVRRCRC 

       250        260        270        280        290        300 
KEGRDYNAPD SKWEIKRCQC CGSSGTHLAC SSLRSWEQNW ECLECRGIIY NSGEFQKAKK 

       310        320        330        340        350        360 
HVLPNSNNVG ITDCLLEESS PKLPRQSPGS QSKDLLRQGS KFRRNVSTLL IELGFQIKKK 

       370        380        390        400        410        420 
TKRLYINKAN IWNSALDAFR NRNFNPSYAI EVAYVIENDN FGSEHPGSKQ EFLSLLMQHL 

       430        440        450        460        470        480 
ENSSLFEGSL SKNLSLNSQA LKENLYYEAG KMLAISLVHG GPSPGFFSKT LFNCLVYGPE 

       490        500        510        520        530        540 
NTQPILDDVS DFDVAQIIIR INTATTVADL KSIINECYNY LELIGCLRLI TTLSDKYMLV 

       550        560        570        580        590        600 
KDILGYHVIQ RVHTPFESFK QGLKTLGVLE KIQAYPEAFC SILCHKPESL SAKILSELFT 

       610        620        630        640        650        660 
VHTLPDVKAL GFWNSYLQAV EDGKSTTTME DILIFATGCS SIPPAGFKPT PSIECLHVDF 

       670        680        690        700 
PVGNKCNNCL AIPITNTYKE FQENMDFTIR NTLRLEKEES SHYIGH 

« Hide

References

« Hide 'large scale' references
[1]"G2E3 is a nucleo-cytoplasmic shuttling protein with DNA damage responsive localization."
Brooks W.S., Banerjee S., Crawford D.F.
Exp. Cell Res. 313:665-676(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF 30-LYS-LYS-31 AND CYS-666.
[2]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], TISSUE SPECIFICITY.
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hepatoma and Teratocarcinoma.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[5]"The G(2) DNA damage checkpoint delays expression of genes encoding mitotic regulators."
Crawford D.F., Piwnica-Worms H.
J. Biol. Chem. 276:37166-37177(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"G2E3 is a dual function ubiquitin ligase required for early embryonic development."
Brooks W.S., Helton E.S., Banerjee S., Venable M., Johnson L., Schoeb T.R., Kesterson R.A., Crawford D.F.
J. Biol. Chem. 283:22304-22315(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DOMAIN, MUTAGENESIS OF CYS-84; CYS-147; CYS-258; CYS-261 AND CYS-666.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB037754 mRNA. Translation: BAA92571.1. Different initiation.
AK000340 mRNA. Translation: BAA91095.1.
AK022867 mRNA. Translation: BAB14280.1. Different initiation.
BC000973 mRNA. Translation: AAH00973.2.
RefSeqNP_060239.2. NM_017769.3.
UniGeneHs.509008.
Hs.605081.

3D structure databases

ProteinModelPortalQ7L622.
SMRQ7L622. Positions 77-130, 214-285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120771. 3 interactions.
IntActQ7L622. 4 interactions.
MINTMINT-1441199.
STRING9606.ENSP00000206595.

PTM databases

PhosphoSiteQ7L622.

Polymorphism databases

DMDM74738611.

Proteomic databases

PaxDbQ7L622.
PRIDEQ7L622.

Protocols and materials databases

DNASU55632.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000206595; ENSP00000206595; ENSG00000092140.
GeneID55632.
KEGGhsa:55632.
UCSCuc001wqk.2. human.

Organism-specific databases

CTD55632.
GeneCardsGC14P031028.
HGNCHGNC:20338. G2E3.
HPAHPA001601.
MIM611299. gene.
neXtProtNX_Q7L622.
PharmGKBPA164720127.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG304942.
HOGENOMHOG000112684.
HOVERGENHBG055656.
InParanoidQ7L622.
OrthoDBEOG78WKSC.
PhylomeDBQ7L622.
TreeFamTF325426.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ7L622.
BgeeQ7L622.
CleanExHS_KIAA1333.
GenevestigatorQ7L622.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR000569. HECT.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF00632. HECT. 1 hit.
[Graphical view]
SMARTSM00249. PHD. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF56204. SSF56204. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEPS50237. HECT. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKIAA1333.
GenomeRNAi55632.
NextBio60275.
PROQ7L622.
SOURCESearch...

Entry information

Entry nameG2E3_HUMAN
AccessionPrimary (citable) accession number: Q7L622
Secondary accession number(s): Q9BVR2 expand/collapse secondary AC list , Q9H9E9, Q9NXC0, Q9P2L3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM