Q7L5Y9 (MAEA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Macrophage erythroblast attacher

Alternative name(s):
Cell proliferation-inducing gene 5 protein
Erythroblast macrophage protein
Human lung cancer oncogene 10 protein
Short name=HLC-10

Gene names

Name:	MAEA
Synonyms:	EMP
ORF Names:	HLC10, PIG5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Play a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. Ref.4 Ref.7
Subunit structure	Forms a complex with F-actin. Ref.7
Subcellular location	Nucleus matrix. Cell membrane. Cytoplasm › cytoskeleton. Note: Localized as nuclear speckled-like pattern. Ref.7
Tissue specificity	Ubiquitous. Ref.4 Ref.7
Developmental stage	Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis. Ref.7
Sequence similarities	Contains 1 CTLH domain. Contains 1 LisH domain.
Sequence caution	The sequence AAC67543.1 differs from that shown. Reason: Erroneous termination at position 397. Translated as stop. The sequence AAC67543.1 differs from that shown. Reason: Frameshift at positions 253 and 327. The sequence AAC67543.1 differs from that shown. Reason: Sequence differs at N-terminus. The sequence AAO85220.1 differs from that shown. Reason: Erroneous initiation. The sequence AK128302 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Biological process	Cell cycle Cell division Erythrocyte maturation
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from direct assay Ref.4. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW erythrocyte maturation Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of myeloid cell apoptosis Inferred from direct assay Ref.4. Source: UniProtKB regulation of mitotic cell cycle Non-traceable author statement Ref.7. Source: UniProtKB
Cellular component	actomyosin contractile ring Inferred from direct assay Ref.7. Source: UniProtKB integral to plasma membrane Inferred from direct assay Ref.4. Source: UniProtKB membrane fraction Inferred from direct assay Ref.4. Source: UniProtKB nuclear matrix Inferred from direct assay Ref.7. Source: UniProtKB spindle Inferred from direct assay Ref.7. Source: UniProtKB
Molecular function	actin binding Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7L5Y9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7L5Y9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     153-193: Missing.
     265-265: M → TCTVAD

Isoform 3 (identifier: Q7L5Y9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     153-193: Missing.

Isoform 4 (identifier: Q7L5Y9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     152-232: EDLVNIEMFL...KHFSQAEGSQ → GTCKKALQPS...PAGWPLSHQD
     233-300: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q7L5Y9-5)

The sequence of this isoform differs from the canonical sequence as follows:
     153-245: DLVNIEMFLT...VRQAMGMLAF → TCKKALQPSR...LQGGRQLQEP
     246-396: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Macrophage erythroblast attacher		PRO_0000284936
Regions
	Domain	121 – 153	33	LisH
	Domain	159 – 216	58	CTLH
	Region	1 – 124	124	Extracellular and involved in cell to cell contact
Natural variations
	Alternative sequence	152 – 232	81	EDLVN…AEGSQ → GTCKKALQPSRREPAGRGAP GHGHAGLPARHAHLPVQGPS GPCTVADADPAVPVRQLPTT PAGKQFCVHPHPAGWPLSHQ D in isoform 4.		VSP_024784
	Alternative sequence	153 – 245	93	DLVNI…GMLAF → TCKKALQPSRREPAGRGAPG HGHAGLPARHAHLPVQGPSG PCTVADADPAVPVRQLPTTP AGKQFCVHPHPAGRPLSHQD TTVLQGGRQLQEP in isoform 5.		VSP_024785
	Alternative sequence	153 – 193	41	Missing in isoform 2 and isoform 3.		VSP_024786
	Alternative sequence	233 – 300	68	Missing in isoform 4.		VSP_024788
	Alternative sequence	246 – 396	151	Missing in isoform 5.		VSP_024789
	Alternative sequence	265	1	M → TCTVAD in isoform 2.		VSP_024790
	Natural variant	34	1	R → C. Corresponds to variant rs34082974 [ dbSNP | Ensembl ].		VAR_051150
Experimental info
	Sequence conflict	32	1	R → C in AAP74806. Ref.1
	Sequence conflict	56	1	K → R in AAC67543. Ref.4
	Sequence conflict	101	1	R → L in AAC67543. Ref.4
	Sequence conflict	123	1	K → R in BAA91499. Ref.2
	Sequence conflict	313	1	D → V in AAC67543. Ref.4
	Sequence conflict	327	1	P → R in AAC67543. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 361FB82BE0240C21	FASTA	396	45,287
	10 20 30 40 50 60 MAVQESAAQL SMTLKVQEYP TLKVPYETLN KRFRAAQKNI DRETSHVTMV VAELEKTLSG 70 80 90 100 110 120 CPAVDSVVSL LDGVVEKLSV LKRKAVESIQ AEDESAKLCK RRIEHLKEHS SDQPAAASVW 130 140 150 160 170 180 KRKRMDRMMV EHLLRCGYYN TAVKLARQSG IEDLVNIEMF LTAKEVEESL ERRETATCLA 190 200 210 220 230 240 WCHDNKSRLR KMKSCLEFSL RIQEFIELIR QNKRLDAVRH ARKHFSQAEG SQLDEVRQAM 250 260 270 280 290 300 GMLAFPPDTH ISPYKDLLDP ARWRMLIQQF RYDNYRLHQL GNNSVFTLTL QAGLSAIKTP 310 320 330 340 350 360 QCYKEDGSSK SPDCPVCSRS LNKLAQPLPM AHCANSRLVC KISGDVMNEN NPPMMLPNGY 370 380 390 VYGYNSLLSI RQDDKVVCPR TKEVFHFSQA EKVYIM
	Isoform 2 [UniParc]. Checksum: 7EDD1E1771B311F0	FASTA	360	40,869
	10 20 30 40 50 60 MAVQESAAQL SMTLKVQEYP TLKVPYETLN KRFRAAQKNI DRETSHVTMV VAELEKTLSG 70 80 90 100 110 120 CPAVDSVVSL LDGVVEKLSV LKRKAVESIQ AEDESAKLCK RRIEHLKEHS SDQPAAASVW 130 140 150 160 170 180 KRKRMDRMMV EHLLRCGYYN TAVKLARQSG IESCLEFSLR IQEFIELIRQ NKRLDAVRHA 190 200 210 220 230 240 RKHFSQAEGS QLDEVRQAMG MLAFPPDTHI SPYKDLLDPA RWRTCTVADL IQQFRYDNYR 250 260 270 280 290 300 LHQLGNNSVF TLTLQAGLSA IKTPQCYKED GSSKSPDCPV CSRSLNKLAQ PLPMAHCANS 310 320 330 340 350 360 RLVCKISGDV MNENNPPMML PNGYVYGYNS LLSIRQDDKV VCPRTKEVFH FSQAEKVYIM
	Isoform 3 [UniParc]. Checksum: C949C3631DCD33E1	FASTA	355	40,410
	10 20 30 40 50 60 MAVQESAAQL SMTLKVQEYP TLKVPYETLN KRFRAAQKNI DRETSHVTMV VAELEKTLSG 70 80 90 100 110 120 CPAVDSVVSL LDGVVEKLSV LKRKAVESIQ AEDESAKLCK RRIEHLKEHS SDQPAAASVW 130 140 150 160 170 180 KRKRMDRMMV EHLLRCGYYN TAVKLARQSG IESCLEFSLR IQEFIELIRQ NKRLDAVRHA 190 200 210 220 230 240 RKHFSQAEGS QLDEVRQAMG MLAFPPDTHI SPYKDLLDPA RWRMLIQQFR YDNYRLHQLG 250 260 270 280 290 300 NNSVFTLTLQ AGLSAIKTPQ CYKEDGSSKS PDCPVCSRSL NKLAQPLPMA HCANSRLVCK 310 320 330 340 350 ISGDVMNENN PPMMLPNGYV YGYNSLLSIR QDDKVVCPRT KEVFHFSQAE KVYIM
	Isoform 4 [UniParc]. Checksum: D7E3935AB82BE82D	FASTA	328	36,122
	10 20 30 40 50 60 MAVQESAAQL SMTLKVQEYP TLKVPYETLN KRFRAAQKNI DRETSHVTMV VAELEKTLSG 70 80 90 100 110 120 CPAVDSVVSL LDGVVEKLSV LKRKAVESIQ AEDESAKLCK RRIEHLKEHS SDQPAAASVW 130 140 150 160 170 180 KRKRMDRMMV EHLLRCGYYN TAVKLARQSG IGTCKKALQP SRREPAGRGA PGHGHAGLPA 190 200 210 220 230 240 RHAHLPVQGP SGPCTVADAD PAVPVRQLPT TPAGKQFCVH PHPAGWPLSH QDQCYKEDGS 250 260 270 280 290 300 SKSPDCPVCS RSLNKLAQPL PMAHCANSRL VCKISGDVMN ENNPPMMLPN GYVYGYNSLL 310 320 SIRQDDKVVC PRTKEVFHFS QAEKVYIM
	Isoform 5 [UniParc]. Checksum: 937AAE595F6C83BF	FASTA	245	26,802
	10 20 30 40 50 60 MAVQESAAQL SMTLKVQEYP TLKVPYETLN KRFRAAQKNI DRETSHVTMV VAELEKTLSG 70 80 90 100 110 120 CPAVDSVVSL LDGVVEKLSV LKRKAVESIQ AEDESAKLCK RRIEHLKEHS SDQPAAASVW 130 140 150 160 170 180 KRKRMDRMMV EHLLRCGYYN TAVKLARQSG IETCKKALQP SRREPAGRGA PGHGHAGLPA 190 200 210 220 230 240 RHAHLPVQGP SGPCTVADAD PAVPVRQLPT TPAGKQFCVH PHPAGRPLSH QDTTVLQGGR QLQEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a human cell proliferation gene 5." Kim J.W. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4). Tissue: Embryo and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[4]	"Molecular identification and functional characterization of a novel protein that mediates the attachment of erythroblasts to macrophages." Hanspal M., Smockova Y., Uong Q. Blood 92:2940-2950(1998) [PubMed: 9763581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-396 (ISOFORM 2), REGION, FUNCTION, TISSUE SPECIFICITY.
[5]	"Identification of new human cancer-related gene (HLC-10)." Kim J.W. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-396 (ISOFORM 1).
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 12-396 (ISOFORM 1).
[7]	"Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division." Bala S., Kumar A., Soni S., Sinha S., Hanspal M. Biochem. Biophys. Res. Commun. 342:1040-1048(2006) [PubMed: 16510120] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBUNIT, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY236486 mRNA. Translation: AAP74806.1. AK001088 mRNA. Translation: BAA91499.1. AK128302 mRNA. No translation available. AK022515 mRNA. Translation: BAB14072.1. AK022586 mRNA. Translation: BAB14113.1. BC001225 mRNA. Translation: AAH01225.2. BC006470 mRNA. Translation: AAH06470.2. AF084928 mRNA. Translation: AAC67543.1. Sequence problems. AY189687 mRNA. Translation: AAO85220.1. Different initiation. BT006957 mRNA. Translation: AAP35603.1.
IPI	IPI00419963. IPI00556323. IPI00607605. IPI00845303. IPI00845323.
RefSeq	NP_001017405.1. NM_001017405.1. NP_005873.2. NM_005882.3.
UniGene	Hs.139896.
3D structure databases
ProteinModelPortal	Q7L5Y9.
SMR	Q7L5Y9. Positions 335-391.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7L5Y9.
PTM databases
PhosphoSite	Q7L5Y9.
Polymorphism databases
DMDM	74754297.
Proteomic databases
PRIDE	Q7L5Y9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000303400; ENSP00000302830; ENSG00000090316.
GeneID	10296.
KEGG	hsa:10296.
NMPDR	fig|9606.3.peg.23794.
UCSC	uc003gda.1. human. uc003gdb.1. human. uc003gdc.1. human.
Organism-specific databases
CTD	10296.
GeneCards	GC04P001273.
H-InvDB	HIX0004018.
HGNC	HGNC:13731. MAEA.
MIM	606801. gene.
neXtProt	NX_Q7L5Y9.
PharmGKB	PA30533.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12004.
GeneTree	ENSGT00550000074989.
HOVERGEN	HBG053270.
InParanoid	Q7L5Y9.
OMA	ENYRLFQ.
OrthoDB	EOG4MPHQ8.
PhylomeDB	Q7L5Y9.
Gene expression databases
ArrayExpress	Q7L5Y9.
Bgee	Q7L5Y9.
CleanEx	HS_MAEA.
Genevestigator	Q7L5Y9.
Family and domain databases
InterPro	IPR006595. CTLH_C. IPR006594. LisH_dimerisation. IPR013144. Ran-bd_prot-like_CRA_domain. [Graphical view]
SMART	SM00757. CRA. 1 hit. SM00668. CTLH. 1 hit. SM00667. LisH. 1 hit. [Graphical view]
PROSITE	PS50897. CTLH. 1 hit. PS50896. LISH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39018.
SOURCE	Search...

Entry information

Entry name

MAEA_HUMAN

Accession

Primary (citable) accession number: Q7L5Y9
Secondary accession number(s): O95285 , Q5JB54, Q6ZRD6, Q9BQ11, Q9H9V6, Q9H9Z4, Q9NW84

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 5, 2005
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.