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Protein

DET1 homolog

Gene

DET1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation.1 Publication

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
DET1 homolog
Alternative name(s):
De-etiolated-1 homolog
Gene namesi
Name:DET1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:25477. DET1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134943899.

Polymorphism and mutation databases

BioMutaiDET1.
DMDMi55976471.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 550550DET1 homologPRO_0000129026Add
BLAST

Proteomic databases

EPDiQ7L5Y6.
MaxQBiQ7L5Y6.
PaxDbiQ7L5Y6.
PeptideAtlasiQ7L5Y6.
PRIDEiQ7L5Y6.

PTM databases

iPTMnetiQ7L5Y6.
PhosphoSiteiQ7L5Y6.

Expressioni

Tissue specificityi

Highly expressed in the ovary, some lymphoid organs and resting leukocytes.1 Publication

Gene expression databases

BgeeiQ7L5Y6.
ExpressionAtlasiQ7L5Y6. baseline and differential.
GenevisibleiQ7L5Y6. HS.

Organism-specific databases

HPAiHPA048873.

Interactioni

Subunit structurei

Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1.1 Publication

Protein-protein interaction databases

BioGridi120387. 30 interactions.
IntActiQ7L5Y6. 13 interactions.
STRINGi9606.ENSP00000408994.

Structurei

3D structure databases

ProteinModelPortaliQ7L5Y6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DET1 family.Curated

Phylogenomic databases

eggNOGiKOG2558. Eukaryota.
ENOG410XSK5. LUCA.
GeneTreeiENSGT00390000005224.
HOGENOMiHOG000007709.
HOVERGENiHBG051339.
InParanoidiQ7L5Y6.
KOiK10571.
OMAiKFKIQAG.
OrthoDBiEOG7PS1F0.
PhylomeDBiQ7L5Y6.
TreeFamiTF324729.

Family and domain databases

InterProiIPR019138. De-etiolated_protein_1_Det1.
[Graphical view]
PfamiPF09737. Det1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7L5Y6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDHHVSTIKP RRIQNQNVIH RLERRRISSG KAGTHWHQVR VFHQNVFPNF
60 70 80 90 100
TVVNVEKPPC FLRKFSPDGR YFIAFSSDQT SLEIYEYQGC QAAEDLLQGY
110 120 130 140 150
EGEILSNGND QRSVNIRGRL FERFFVLLHI TNVAANGEHL NRECSLFTDD
160 170 180 190 200
CRCVIVGSAA YLPDEPHPPF FEVYRNSESV TPNPRSPLED YSLHIIDLHT
210 220 230 240 250
GRLCDTRTFK CDKVVLSHNQ GLYLYKNILA ILSVQQQTIH VFQVTPEGTF
260 270 280 290 300
IDVRTIGRFC YEDDLLTVSA VFPEVQRDSQ TGMANPFRDP FINSLKHRLL
310 320 330 340 350
VYLWRRAEQD GSAMAKRRFF QYFDQLRQLR MWKMQLLDEN HLFIKYTSED
360 370 380 390 400
VVTLRVTDPS QASFFVVYNM VTTEVIAVFE NTSDELLELF ENFCDLFRNA
410 420 430 440 450
TLHSEVQFPC SASSNNFARQ IQRRFKDTII NAKYGGHTEA VRRLLGQLPI
460 470 480 490 500
SAQSYSGSPY LDLSLFSYDD KWVSVMERPK TCGDHPIRFY ARDSGLLKFE
510 520 530 540 550
IQAGLLGRPI NHTVRRLVAF TFHPFEPFAI SVQRTNAEYV VNFHMRHCCT
Length:550
Mass (Da):63,848
Last modified:November 23, 2004 - v2
Checksum:iA164AC844F897DF7
GO
Isoform 2 (identifier: Q7L5Y6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVGQILKRDVIM

Note: No experimental confirmation available.
Show »
Length:561
Mass (Da):65,102
Checksum:i733F7156B8561988
GO

Sequence cautioni

The sequence BAA91447.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MVGQILKRDVIM in isoform 2. 1 PublicationVSP_043012

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000965 mRNA. Translation: BAA91447.1. Different initiation.
AK054603 mRNA. Translation: BAG51398.1.
BC001242 mRNA. Translation: AAH01242.2.
BC109060 mRNA. Translation: AAI09061.1.
BC109061 mRNA. Translation: AAI09062.1.
CCDSiCCDS45343.1. [Q7L5Y6-2]
CCDS45344.1. [Q7L5Y6-1]
RefSeqiNP_001137546.1. NM_001144074.2. [Q7L5Y6-1]
NP_001308525.1. NM_001321596.1. [Q7L5Y6-1]
NP_060466.2. NM_017996.4. [Q7L5Y6-2]
XP_011520049.1. XM_011521747.1. [Q7L5Y6-2]
XP_011520050.1. XM_011521748.1. [Q7L5Y6-1]
UniGeneiHs.655064.
Hs.690871.

Genome annotation databases

EnsembliENST00000268148; ENSP00000268148; ENSG00000140543. [Q7L5Y6-1]
ENST00000444300; ENSP00000408994; ENSG00000140543. [Q7L5Y6-2]
ENST00000564406; ENSP00000456340; ENSG00000140543. [Q7L5Y6-2]
GeneIDi55070.
KEGGihsa:55070.
UCSCiuc002bmq.3. human. [Q7L5Y6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000965 mRNA. Translation: BAA91447.1. Different initiation.
AK054603 mRNA. Translation: BAG51398.1.
BC001242 mRNA. Translation: AAH01242.2.
BC109060 mRNA. Translation: AAI09061.1.
BC109061 mRNA. Translation: AAI09062.1.
CCDSiCCDS45343.1. [Q7L5Y6-2]
CCDS45344.1. [Q7L5Y6-1]
RefSeqiNP_001137546.1. NM_001144074.2. [Q7L5Y6-1]
NP_001308525.1. NM_001321596.1. [Q7L5Y6-1]
NP_060466.2. NM_017996.4. [Q7L5Y6-2]
XP_011520049.1. XM_011521747.1. [Q7L5Y6-2]
XP_011520050.1. XM_011521748.1. [Q7L5Y6-1]
UniGeneiHs.655064.
Hs.690871.

3D structure databases

ProteinModelPortaliQ7L5Y6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120387. 30 interactions.
IntActiQ7L5Y6. 13 interactions.
STRINGi9606.ENSP00000408994.

PTM databases

iPTMnetiQ7L5Y6.
PhosphoSiteiQ7L5Y6.

Polymorphism and mutation databases

BioMutaiDET1.
DMDMi55976471.

Proteomic databases

EPDiQ7L5Y6.
MaxQBiQ7L5Y6.
PaxDbiQ7L5Y6.
PeptideAtlasiQ7L5Y6.
PRIDEiQ7L5Y6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268148; ENSP00000268148; ENSG00000140543. [Q7L5Y6-1]
ENST00000444300; ENSP00000408994; ENSG00000140543. [Q7L5Y6-2]
ENST00000564406; ENSP00000456340; ENSG00000140543. [Q7L5Y6-2]
GeneIDi55070.
KEGGihsa:55070.
UCSCiuc002bmq.3. human. [Q7L5Y6-1]

Organism-specific databases

CTDi55070.
GeneCardsiDET1.
HGNCiHGNC:25477. DET1.
HPAiHPA048873.
MIMi608727. gene.
neXtProtiNX_Q7L5Y6.
PharmGKBiPA134943899.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2558. Eukaryota.
ENOG410XSK5. LUCA.
GeneTreeiENSGT00390000005224.
HOGENOMiHOG000007709.
HOVERGENiHBG051339.
InParanoidiQ7L5Y6.
KOiK10571.
OMAiKFKIQAG.
OrthoDBiEOG7PS1F0.
PhylomeDBiQ7L5Y6.
TreeFamiTF324729.

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii55070.
PROiQ7L5Y6.
SOURCEiSearch...

Gene expression databases

BgeeiQ7L5Y6.
ExpressionAtlasiQ7L5Y6. baseline and differential.
GenevisibleiQ7L5Y6. HS.

Family and domain databases

InterProiIPR019138. De-etiolated_protein_1_Det1.
[Graphical view]
PfamiPF09737. Det1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryo and Neuroblastoma.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. "Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase."
    Wertz I.E., O'Rourke K.M., Zhang Z., Dornan D., Arnott D., Deshaies R.J., Dixit V.M.
    Science 303:1371-1374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, IDENTIFICATION IN THE DCX DET1-COP1 COMPLEX WITH RBX1; CUL4A; COP1 AND DDB1.

Entry informationi

Entry nameiDET1_HUMAN
AccessioniPrimary (citable) accession number: Q7L5Y6
Secondary accession number(s): B3KNN6, Q2VPC0, Q9NWD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: July 6, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.