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Protein

Mitochondrial enolase superfamily member 1

Gene

ENOSF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion (PubMed:24697329).1 Publication

Catalytic activityi

L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

kcat is 0.5 sec(-1) for L-fuconate. kcat is 0.3 sec(-1) for L-galactonate. kcat is 0.3 sec(-1) for L-arabinonate. kcat is 0.04 sec(-1) for D-arabinonate. kcat is 0.002 sec(-1) for D-ribonate.

  1. KM=0.2 mM for L-fuconate1 Publication
  2. KM=3.0 mM for L-galactonate1 Publication
  3. KM=2.0 mM for D-arabinonate1 Publication
  4. KM=4.0 mM for L-arabinonate1 Publication
  5. KM=0.4 mM for D-ribonate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341SubstrateBy similarity
    Binding sitei220 – 2201SubstrateBy similarity
    Active sitei222 – 2221Proton donor/acceptorBy similarity
    Metal bindingi250 – 2501Magnesium1 Publication
    Binding sitei252 – 2521SubstrateBy similarity
    Metal bindingi276 – 2761Magnesium1 Publication
    Binding sitei276 – 2761SubstrateBy similarity
    Metal bindingi305 – 3051Magnesium1 Publication
    Binding sitei305 – 3051SubstrateBy similarity
    Active sitei355 – 3551Sequence Analysis
    Binding sitei386 – 3861SubstrateBy similarity

    GO - Molecular functioni

    • isomerase activity Source: UniProtKB-KW
    • L-fuconate dehydratase activity Source: UniProtKB
    • magnesium ion binding Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase, Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial enolase superfamily member 1 (EC:4.2.1.681 Publication)
    Alternative name(s):
    Antisense RNA to thymidylate synthase
    Short name:
    rTS
    L-fuconate dehydratase
    Gene namesi
    Name:ENOSF1
    Synonyms:RTS, TYMSAS
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:30365. ENOSF1.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1 – 2727Missing : Impairs protein solubility. Abolishes catalytic activity. 1 PublicationAdd
    BLAST

    Organism-specific databases

    PharmGKBiPA134897613.

    Polymorphism and mutation databases

    BioMutaiENOSF1.
    DMDMi74739173.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Mitochondrial enolase superfamily member 1PRO_0000331652Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei148 – 1481Phosphoserine1 Publication

    Post-translational modificationi

    Could be sumoylated.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ7L5Y1.
    PaxDbiQ7L5Y1.
    PRIDEiQ7L5Y1.

    PTM databases

    PhosphoSiteiQ7L5Y1.

    Expressioni

    Gene expression databases

    BgeeiQ7L5Y1.
    CleanExiHS_ENOSF1.
    ExpressionAtlasiQ7L5Y1. baseline and differential.
    GenevisibleiQ7L5Y1. HS.

    Organism-specific databases

    HPAiHPA047829.

    Interactioni

    Protein-protein interaction databases

    IntActiQ7L5Y1. 1 interaction.
    STRINGi9606.ENSP00000345974.

    Structurei

    Secondary structure

    1
    443
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 1511Combined sources
    Helixi18 – 203Combined sources
    Beta strandi35 – 4511Combined sources
    Beta strandi51 – 577Combined sources
    Helixi62 – 7211Combined sources
    Helixi73 – 753Combined sources
    Turni76 – 783Combined sources
    Helixi81 – 855Combined sources
    Helixi88 – 969Combined sources
    Helixi101 – 1044Combined sources
    Beta strandi106 – 1083Combined sources
    Helixi109 – 13022Combined sources
    Helixi134 – 1407Combined sources
    Helixi143 – 1475Combined sources
    Turni153 – 1586Combined sources
    Helixi161 – 17010Combined sources
    Turni171 – 1744Combined sources
    Helixi175 – 18511Combined sources
    Beta strandi187 – 1915Combined sources
    Helixi201 – 21313Combined sources
    Beta strandi218 – 2225Combined sources
    Helixi227 – 24115Combined sources
    Beta strandi245 – 2506Combined sources
    Helixi257 – 26711Combined sources
    Helixi268 – 2703Combined sources
    Beta strandi273 – 2764Combined sources
    Helixi284 – 29411Combined sources
    Helixi295 – 2973Combined sources
    Beta strandi300 – 3034Combined sources
    Helixi310 – 3189Combined sources
    Beta strandi323 – 3253Combined sources
    Turni329 – 3313Combined sources
    Helixi334 – 34714Combined sources
    Turni358 – 3603Combined sources
    Helixi361 – 37515Combined sources
    Beta strandi385 – 3873Combined sources
    Helixi392 – 3943Combined sources
    Beta strandi395 – 3973Combined sources
    Beta strandi400 – 4078Combined sources
    Beta strandi411 – 4133Combined sources
    Helixi420 – 4267Combined sources
    Turni428 – 4303Combined sources
    Helixi432 – 4376Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4A35X-ray1.74A1-440[»]
    ProteinModelPortaliQ7L5Y1.
    SMRiQ7L5Y1. Positions 1-440.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 263Substrate bindingBy similarity
    Regioni355 – 3573Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG4948.
    GeneTreeiENSGT00390000014290.
    HOGENOMiHOG000113757.
    HOVERGENiHBG053904.
    InParanoidiQ7L5Y1.
    KOiK18334.
    OMAiSAAYCIL.
    OrthoDBiEOG77DJ5S.
    PhylomeDBiQ7L5Y1.
    TreeFamiTF300529.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N-like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N_dom.
    IPR001354. MR/MLE/MAL.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    PROSITEiPS00909. MR_MLE_2. 1 hit.
    [Graphical view]

    Sequences (7)i

    Sequence statusi: Complete.

    This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q7L5Y1-1) [UniParc]FASTAAdd to basket

    Also known as: rTSgamma

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MVRGRISRLS VRDVRFPTSL GGHGADAMHT DPDYSAAYVV IETDAEDGIK
    60 70 80 90 100
    GCGITFTLGK GTEVVVCAVN ALAHHVLNKD LKDIVGDFRG FYRQLTSDGQ
    110 120 130 140 150
    LRWIGPEKGV VHLATAAVLN AVWDLWAKQE GKPVWKLLVD MDPRMLVSCI
    160 170 180 190 200
    DFRYITDVLT EEDALEILQK GQIGKKEREK QMLAQGYPAY TTSCAWLGYS
    210 220 230 240 250
    DDTLKQLCAQ ALKDGWTRFK VKVGADLQDD MRRCQIIRDM IGPEKTLMMD
    260 270 280 290 300
    ANQRWDVPEA VEWMSKLAKF KPLWIEEPTS PDDILGHATI SKALVPLGIG
    310 320 330 340 350
    IATGEQCHNR VIFKQLLQAK ALQFLQIDSC RLGSVNENLS VLLMAKKFEI
    360 370 380 390 400
    PVCPHAGGVG LCELVQHLII FDYISVSASL ENRVCEYVDH LHEHFKYPVM
    410 420 430 440
    IQRASYMPPK DPGYSTEMKE ESVKKHQYPD GEVWKKLLPA QEN
    Length:443
    Mass (Da):49,786
    Last modified:May 24, 2005 - v1
    Checksum:i82BEDAC229D1A729
    GO
    Isoform 2 (identifier: Q7L5Y1-2) [UniParc]FASTAAdd to basket

    Also known as: rTSalpha

    The sequence of this isoform differs from the canonical sequence as follows:
         1-102: Missing.
         103-103: W → MQKMESRGVELPSLWEKALKL

    Show »
    Length:361
    Mass (Da):41,024
    Checksum:i73B469F1C9C7E144
    GO
    Isoform 3 (identifier: Q7L5Y1-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         65-117: VVCAVNALAH...KGVVHLATAA → DWSRKGRGAP...GILHRFQVHH
         118-443: Missing.

    Show »
    Length:117
    Mass (Da):12,286
    Checksum:iB277BFA534E7DAF2
    GO
    Isoform 4 (identifier: Q7L5Y1-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-24: MVRGRISRLSVRDVRFPTSLGGHG → MVSADAMVSADAMVSADAMVSADAMVSADAMVSADAMVSADAMVS
         293-306: Missing.

    Note: Gene prediction based on EST data.
    Show »
    Length:450
    Mass (Da):50,170
    Checksum:i7D6BD13DF0F12AD0
    GO
    Isoform 5 (identifier: Q7L5Y1-5) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-24: MVRGRISRLSVRDVRFPTSLGGHG → MVSADAMVSA...AMVSADAMVS
         293-306: Missing.

    Show »
    Length:474
    Mass (Da):52,468
    Checksum:i97FA6018CAADF8CB
    GO
    Isoform 6 (identifier: Q7L5Y1-6) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-181: Missing.

    Show »
    Length:262
    Mass (Da):29,710
    Checksum:i5296CB2DCA806273
    GO
    Isoform 7 (identifier: Q7L5Y1-7) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-230: Missing.

    Show »
    Length:213
    Mass (Da):24,284
    Checksum:iF2CF0580E05E10BA
    GO

    Sequence cautioni

    The sequence AAG29537.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence CAA47471.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311D → E.
    Corresponds to variant rs34724061 [ dbSNP | Ensembl ].
    VAR_042933
    Natural varianti145 – 1451M → T.4 Publications
    Corresponds to variant rs2612086 [ dbSNP | Ensembl ].
    VAR_042934
    Natural varianti428 – 4281Y → S.
    Corresponds to variant rs2847620 [ dbSNP | Ensembl ].
    VAR_042935

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 230230Missing in isoform 7. 1 PublicationVSP_055241Add
    BLAST
    Alternative sequencei1 – 181181Missing in isoform 6. 1 PublicationVSP_055242Add
    BLAST
    Alternative sequencei1 – 102102Missing in isoform 2. 1 PublicationVSP_033311Add
    BLAST
    Alternative sequencei1 – 2424MVRGR…LGGHG → MVSADAMVSADAMVSADAMV SADAMVSADAMVSADAMVSA DAMVS in isoform 4. CuratedVSP_047153Add
    BLAST
    Alternative sequencei1 – 2424MVRGR…LGGHG → MVSADAMVSADAMVSADAMV SADAMVSADAMVSADAMVSA DAMVSADAMVSADAMVSADA MVSADAMVS in isoform 5. 1 PublicationVSP_055243Add
    BLAST
    Alternative sequencei65 – 11753VVCAV…LATAA → DWSRKGRGAPGDSGRPKRGV GLVGQAGGKACLEVTCGHGS QDAGILHRFQVHH in isoform 3. 1 PublicationVSP_033312Add
    BLAST
    Alternative sequencei103 – 1031W → MQKMESRGVELPSLWEKALK L in isoform 2. 1 PublicationVSP_033313
    Alternative sequencei118 – 443326Missing in isoform 3. 1 PublicationVSP_033314Add
    BLAST
    Alternative sequencei293 – 30614Missing in isoform 4 and isoform 5. 1 PublicationVSP_047154Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF305057 Genomic DNA. Translation: AAG29536.1.
    X67098 mRNA. Translation: CAA47472.1.
    AF305057 Genomic DNA. Translation: AAG29537.1. Different initiation.
    X67098 mRNA. Translation: CAA47471.1. Different initiation.
    X89602 mRNA. Translation: CAA61761.1.
    AK127818 mRNA. Translation: BAC87148.1.
    AK093873 mRNA. Translation: BAG52778.1.
    AK127219 mRNA. Translation: BAG54456.1.
    AK292780 mRNA. Translation: BAF85469.1.
    AP001178 Genomic DNA. No translation available.
    CH471113 Genomic DNA. Translation: EAX01713.1.
    CH471113 Genomic DNA. Translation: EAX01714.1.
    CH471113 Genomic DNA. Translation: EAX01715.1.
    BC001285 mRNA. Translation: AAH01285.2.
    CCDSiCCDS11822.1. [Q7L5Y1-1]
    CCDS11823.1. [Q7L5Y1-4]
    CCDS45821.1. [Q7L5Y1-2]
    RefSeqiNP_001119595.1. NM_001126123.3. [Q7L5Y1-2]
    NP_059982.2. NM_017512.5. [Q7L5Y1-1]
    NP_974487.1. NM_202758.3. [Q7L5Y1-4]
    XP_005258175.1. XM_005258118.2. [Q7L5Y1-6]
    UniGeneiHs.658550.
    Hs.731510.
    Hs.732707.

    Genome annotation databases

    EnsembliENST00000251101; ENSP00000251101; ENSG00000132199.
    ENST00000340116; ENSP00000345974; ENSG00000132199. [Q7L5Y1-4]
    ENST00000383578; ENSP00000373072; ENSG00000132199. [Q7L5Y1-2]
    GeneIDi55556.
    KEGGihsa:55556.
    UCSCiuc002kkt.4. human. [Q7L5Y1-2]
    uc002kku.4. human. [Q7L5Y1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF305057 Genomic DNA. Translation: AAG29536.1.
    X67098 mRNA. Translation: CAA47472.1.
    AF305057 Genomic DNA. Translation: AAG29537.1. Different initiation.
    X67098 mRNA. Translation: CAA47471.1. Different initiation.
    X89602 mRNA. Translation: CAA61761.1.
    AK127818 mRNA. Translation: BAC87148.1.
    AK093873 mRNA. Translation: BAG52778.1.
    AK127219 mRNA. Translation: BAG54456.1.
    AK292780 mRNA. Translation: BAF85469.1.
    AP001178 Genomic DNA. No translation available.
    CH471113 Genomic DNA. Translation: EAX01713.1.
    CH471113 Genomic DNA. Translation: EAX01714.1.
    CH471113 Genomic DNA. Translation: EAX01715.1.
    BC001285 mRNA. Translation: AAH01285.2.
    CCDSiCCDS11822.1. [Q7L5Y1-1]
    CCDS11823.1. [Q7L5Y1-4]
    CCDS45821.1. [Q7L5Y1-2]
    RefSeqiNP_001119595.1. NM_001126123.3. [Q7L5Y1-2]
    NP_059982.2. NM_017512.5. [Q7L5Y1-1]
    NP_974487.1. NM_202758.3. [Q7L5Y1-4]
    XP_005258175.1. XM_005258118.2. [Q7L5Y1-6]
    UniGeneiHs.658550.
    Hs.731510.
    Hs.732707.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4A35X-ray1.74A1-440[»]
    ProteinModelPortaliQ7L5Y1.
    SMRiQ7L5Y1. Positions 1-440.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ7L5Y1. 1 interaction.
    STRINGi9606.ENSP00000345974.

    PTM databases

    PhosphoSiteiQ7L5Y1.

    Polymorphism and mutation databases

    BioMutaiENOSF1.
    DMDMi74739173.

    Proteomic databases

    MaxQBiQ7L5Y1.
    PaxDbiQ7L5Y1.
    PRIDEiQ7L5Y1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000251101; ENSP00000251101; ENSG00000132199.
    ENST00000340116; ENSP00000345974; ENSG00000132199. [Q7L5Y1-4]
    ENST00000383578; ENSP00000373072; ENSG00000132199. [Q7L5Y1-2]
    GeneIDi55556.
    KEGGihsa:55556.
    UCSCiuc002kkt.4. human. [Q7L5Y1-2]
    uc002kku.4. human. [Q7L5Y1-1]

    Organism-specific databases

    CTDi55556.
    GeneCardsiGC18M000664.
    HGNCiHGNC:30365. ENOSF1.
    HPAiHPA047829.
    MIMi607427. gene.
    neXtProtiNX_Q7L5Y1.
    PharmGKBiPA134897613.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiCOG4948.
    GeneTreeiENSGT00390000014290.
    HOGENOMiHOG000113757.
    HOVERGENiHBG053904.
    InParanoidiQ7L5Y1.
    KOiK18334.
    OMAiSAAYCIL.
    OrthoDBiEOG77DJ5S.
    PhylomeDBiQ7L5Y1.
    TreeFamiTF300529.

    Miscellaneous databases

    ChiTaRSiENOSF1. human.
    GenomeRNAii55556.
    NextBioi13632971.
    PROiQ7L5Y1.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ7L5Y1.
    CleanExiHS_ENOSF1.
    ExpressionAtlasiQ7L5Y1. baseline and differential.
    GenevisibleiQ7L5Y1. HS.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N-like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N_dom.
    IPR001354. MR/MLE/MAL.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    PROSITEiPS00909. MR_MLE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and characterization of a naturally occurring antisense RNA to human thymidylate synthase mRNA."
      Dolnick B.J.
      Nucleic Acids Res. 21:1747-1752(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), VARIANT THR-145.
      Tissue: Cervix carcinoma.
    2. "Alternate splicing of the rTS gene product and its overexpression in a 5-fluorouracil-resistant cell line."
      Dolnick B.J., Black A.R.
      Cancer Res. 56:3207-3210(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 23-443 (ISOFORM 3), VARIANT THR-145.
      Tissue: Cervix carcinoma.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 5; 6 AND 7), VARIANT THR-145.
      Tissue: Trachea.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-145.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    7. "rTS gene expression is associated with altered cell sensitivity to thymidylate synthase inhibitors."
      Dolnick B.J., Black A.R., Winkler P.M., Schindler K., Hsueh C.-T.
      Adv. Enzyme Regul. 36:165-180(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: ORIGINALLY PROPOSED FUNCTION.
    8. "Natural antisense (rTSalpha) RNA induces site-specific cleavage of thymidylate synthase mRNA."
      Chu J., Dolnick B.J.
      Biochim. Biophys. Acta 1587:183-193(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ORIGINALLY PROPOSED FUNCTION.
    9. "Comparative genomic analysis reveals a novel mitochondrial isoform of human rTS protein and unusual phylogenetic distribution of the rTS gene."
      Liang P., Nair J.R., Song L., McGuire J.J., Dolnick B.J.
      BMC Genomics 6:125-125(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "Enhancement of 5-fluorouracil sensitivity by an rTS signaling mimic in H630 colon cancer cells."
      Dolnick R., Wu Q., Angelino N.J., Stephanie L.V., Chow K.-C., Sufrin J.R., Dolnick B.J.
      Cancer Res. 65:5917-5924(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-148, SUMOYLATION.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
      Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
      J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    13. "Enzymatic and structural characterization of rTSgamma provides insights into the function of rTSbeta."
      Wichelecki D.J., Froese D.S., Kopec J., Muniz J.R., Yue W.W., Gerlt J.A.
      Biochemistry 53:2732-2738(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF 1-440 IN COMPLEX WITH MAGNESIUM, CATALYTIC ACTIVITY, FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF 1-MET--HIS-27.

    Entry informationi

    Entry nameiENOF1_HUMAN
    AccessioniPrimary (citable) accession number: Q7L5Y1
    Secondary accession number(s): A6NMP3
    , A8K9R5, B3KSL6, B3KXE4, D3DUH0, Q15407, Q15594, Q15595, Q6ZS08, Q9HAS5, Q9HAS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 24, 2005
    Last modified: July 22, 2015
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Was originally (PubMed:8493092) identified as a gene coding for an antisense RNA to thymidylate synthase, and was proposed to down-regulate TYMS activity (PubMed:8869746), possibly by promoting the degradation of TYMS mRNA via an antisense RNA-based mechanism (PubMed:12084460).3 Publications

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.