Q7L5Y1 (ENOF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial enolase superfamily member 1
EC=5.-.-.-

Alternative name(s):
Antisense RNA to thymidylate synthase
Short name=rTS

Gene names

Name:	ENOSF1
Synonyms:	RTS, TYMSAS

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate thymidylate synthase activity. Ref.6 Ref.7
Cofactor	Divalent metal ions. Magnesium seems to be the preferred ion By similarity.
Subcellular location	Mitochondrion Probable Ref.8.
Post-translational modification	Could be sumoylated. Ref.9
Sequence similarities	Belongs to the mandelate racemase/muconate lactonizing enzyme family. ENOSF1 subfamily.
Caution	Was originally (Ref.1) identified as a gene coding for an antisense RNA to thymidylate synthase.
Sequence caution	The sequence AAG29537.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA47471.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular amino acid catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7L5Y1-1)

Also known as: rTSgamma;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7L5Y1-2)

Also known as: rTSalpha;

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.
     103-103: W → MQKMESRGVELPSLWEKALKL

Isoform 3 (identifier: Q7L5Y1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     65-117: VVCAVNALAH...KGVVHLATAA → DWSRKGRGAP...GILHRFQVHH
     118-443: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 443

443

Mitochondrial enolase superfamily member 1

PRO_0000331652

Sites

Active site

222

1

By similarity

Active site

355

1

By similarity

Metal binding

250

1

Magnesium By similarity

Metal binding

276

1

Magnesium By similarity

Metal binding

305

1

Magnesium By similarity

Amino acid modifications

Modified residue

148

1

Phosphoserine Ref.9

Natural variations

Alternative sequence

1 – 102

102

Missing in isoform 2.

VSP_033311

Alternative sequence

65 – 117

53

VVCAV…LATAA → DWSRKGRGAPGDSGRPKRGV GLVGQAGGKACLEVTCGHGS QDAGILHRFQVHH in isoform 3.

VSP_033312

Alternative sequence

103

1

W → MQKMESRGVELPSLWEKALK L in isoform 2.

VSP_033313

Alternative sequence

118 – 443

326

Missing in isoform 3.

VSP_033314

Natural variant

31

1

D → E.
Corresponds to variant rs34724061 [ dbSNP | Ensembl ].

VAR_042933

Natural variant

145

1

M → T. Ref.1 Ref.2 Ref.3 Ref.4
Corresponds to variant rs2612086 [ dbSNP | Ensembl ].

VAR_042934

Natural variant

428

1

Y → S.
Corresponds to variant rs2847620 [ dbSNP | Ensembl ].

VAR_042935

Secondary structure

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443

Helix Strand Turn

Details...

Beta strand

5 – 15

11

Helix

18 – 20

3

Beta strand

35 – 45

11

Beta strand

51 – 57

7

Helix

62 – 72

11

Helix

73 – 75

3

Turn

76 – 78

3

Helix

81 – 85

5

Helix

88 – 96

9

Helix

101 – 104

4

Beta strand

106 – 108

3

Helix

109 – 130

22

Helix

134 – 140

7

Helix

143 – 147

5

Turn

153 – 158

6

Helix

161 – 170

10

Turn

171 – 174

4

Helix

175 – 185

11

Beta strand

187 – 191

5

Helix

201 – 213

13

Beta strand

218 – 222

5

Helix

227 – 241

15

Beta strand

245 – 250

6

Helix

257 – 267

11

Helix

268 – 270

3

Beta strand

273 – 276

4

Helix

284 – 294

11

Helix

295 – 297

3

Beta strand

300 – 303

4

Helix

310 – 318

9

Beta strand

323 – 325

3

Turn

329 – 331

3

Helix

334 – 347

14

Turn

358 – 360

3

Helix

361 – 375

15

Beta strand

385 – 387

3

Helix

392 – 394

3

Beta strand

395 – 397

3

Beta strand

400 – 407

8

Beta strand

411 – 413

3

Helix

420 – 426

7

Turn

428 – 430

3

Helix

432 – 437

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (rTSgamma) [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 82BEDAC229D1A729	FASTA	443	49,786
	10 20 30 40 50 60 MVRGRISRLS VRDVRFPTSL GGHGADAMHT DPDYSAAYVV IETDAEDGIK GCGITFTLGK 70 80 90 100 110 120 GTEVVVCAVN ALAHHVLNKD LKDIVGDFRG FYRQLTSDGQ LRWIGPEKGV VHLATAAVLN 130 140 150 160 170 180 AVWDLWAKQE GKPVWKLLVD MDPRMLVSCI DFRYITDVLT EEDALEILQK GQIGKKEREK 190 200 210 220 230 240 QMLAQGYPAY TTSCAWLGYS DDTLKQLCAQ ALKDGWTRFK VKVGADLQDD MRRCQIIRDM 250 260 270 280 290 300 IGPEKTLMMD ANQRWDVPEA VEWMSKLAKF KPLWIEEPTS PDDILGHATI SKALVPLGIG 310 320 330 340 350 360 IATGEQCHNR VIFKQLLQAK ALQFLQIDSC RLGSVNENLS VLLMAKKFEI PVCPHAGGVG 370 380 390 400 410 420 LCELVQHLII FDYISVSASL ENRVCEYVDH LHEHFKYPVM IQRASYMPPK DPGYSTEMKE 430 440 ESVKKHQYPD GEVWKKLLPA QEN
	Isoform 2 (rTSalpha) [UniParc]. Checksum: 73B469F1C9C7E144	FASTA	361	41,024
	10 20 30 40 50 60 MQKMESRGVE LPSLWEKALK LIGPEKGVVH LATAAVLNAV WDLWAKQEGK PVWKLLVDMD 70 80 90 100 110 120 PRMLVSCIDF RYITDVLTEE DALEILQKGQ IGKKEREKQM LAQGYPAYTT SCAWLGYSDD 130 140 150 160 170 180 TLKQLCAQAL KDGWTRFKVK VGADLQDDMR RCQIIRDMIG PEKTLMMDAN QRWDVPEAVE 190 200 210 220 230 240 WMSKLAKFKP LWIEEPTSPD DILGHATISK ALVPLGIGIA TGEQCHNRVI FKQLLQAKAL 250 260 270 280 290 300 QFLQIDSCRL GSVNENLSVL LMAKKFEIPV CPHAGGVGLC ELVQHLIIFD YISVSASLEN 310 320 330 340 350 360 RVCEYVDHLH EHFKYPVMIQ RASYMPPKDP GYSTEMKEES VKKHQYPDGE VWKKLLPAQE N
	Isoform 3 [UniParc]. Checksum: B277BFA534E7DAF2	FASTA	117	12,286
	10 20 30 40 50 60 MVRGRISRLS VRDVRFPTSL GGHGADAMHT DPDYSAAYVV IETDAEDGIK GCGITFTLGK 70 80 90 100 110 GTEVDWSRKG RGAPGDSGRP KRGVGLVGQA GGKACLEVTC GHGSQDAGIL HRFQVHH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a naturally occurring antisense RNA to human thymidylate synthase mRNA." Dolnick B.J. Nucleic Acids Res. 21:1747-1752(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), VARIANT THR-145. Tissue: Cervix carcinoma.
[2]	"Alternate splicing of the rTS gene product and its overexpression in a 5-fluorouracil-resistant cell line." Dolnick B.J., Black A.R. Cancer Res. 56:3207-3210(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 23-443 (ISOFORM 3), VARIANT THR-145. Tissue: Cervix carcinoma.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-145. Tissue: Trachea.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-145.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	"rTS gene expression is associated with altered cell sensitivity to thymidylate synthase inhibitors." Dolnick B.J., Black A.R., Winkler P.M., Schindler K., Hsueh C.-T. Adv. Enzyme Regul. 36:165-180(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"A novel function for the rTS gene." Dolnick B.J., Angelino N.J., Dolnick R., Sufrin J.R. Cancer Biol. Ther. 2:364-369(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Comparative genomic analysis reveals a novel mitochondrial isoform of human rTS protein and unusual phylogenetic distribution of the rTS gene." Liang P., Nair J.R., Song L., McGuire J.J., Dolnick B.J. BMC Genomics 6:125-125(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Enhancement of 5-fluorouracil sensitivity by an rTS signaling mimic in H630 colon cancer cells." Dolnick R., Wu Q., Angelino N.J., Stephanie L.V., Chow K.-C., Sufrin J.R., Dolnick B.J. Cancer Res. 65:5917-5924(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-148, SUMOYLATION.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF305057 Genomic DNA. Translation: AAG29536.1.
X67098 mRNA. Translation: CAA47472.1.
AF305057 Genomic DNA. Translation: AAG29537.1. Different initiation.
X67098 mRNA. Translation: CAA47471.1. Different initiation.
X89602 mRNA. Translation: CAA61761.1.
AK292780 mRNA. Translation: BAF85469.1.
CH471113 Genomic DNA. Translation: EAX01713.1.
CH471113 Genomic DNA. Translation: EAX01714.1.
CH471113 Genomic DNA. Translation: EAX01715.1.
BC001285 mRNA. Translation: AAH01285.2.

IPI

IPI00184190.
IPI00300601.
IPI00890831.

RefSeq

NP_001119595.1. NM_001126123.3.
NP_059982.2. NM_017512.5.
NP_974487.1. NM_202758.3.

UniGene

Hs.658550.
Hs.731510.
Hs.732707.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4A35	X-ray	1.74	A	1-440	[»]

ProteinModelPortal

Q7L5Y1.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q7L5Y1. 1 interaction.

STRING

9606.ENSP00000345974.

PTM databases

PhosphoSite

Q7L5Y1.

Polymorphism databases

DMDM

74739173.

Proteomic databases

PaxDb

Q7L5Y1.

PRIDE

Q7L5Y1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000251101; ENSP00000251101; ENSG00000132199.
ENST00000383578; ENSP00000373072; ENSG00000132199.
ENST00000585128; ENSP00000462925; ENSG00000132199.

GeneID

55556.

KEGG

hsa:55556.

UCSC

uc002kkt.4. human.
uc002kku.4. human.

Organism-specific databases

CTD

55556.

GeneCards

GC18M000664.

HGNC

HGNC:30365. ENOSF1.

HPA

HPA047829.

MIM

607427. gene.

neXtProt

NX_Q7L5Y1.

PharmGKB

PA134897613.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG4948.

HOVERGEN

HBG053904.

OrthoDB

EOG498V0X.

Gene expression databases

ArrayExpress

Q7L5Y1.

Bgee

Q7L5Y1.

CleanEx

HS_ENOSF1.

Genevestigator

Q7L5Y1.

Family and domain databases

InterPro

IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]

PANTHER

PTHR13794. PTHR13794. 1 hit.

Pfam

PF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]

SMART

SM00922. MR_MLE. 1 hit.
[Graphical view]

PROSITE

PS00908. MR_MLE_1. False negative.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

ENOSF1. human.

GenomeRNAi

55556.

NextBio

60026.

SOURCE

Search...

Entry information

Entry name

ENOF1_HUMAN

Accession

Primary (citable) accession number: Q7L5Y1
Secondary accession number(s): A8K9R5 , D3DUH0, Q15407, Q15594, Q15595, Q9HAS5, Q9HAS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.