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Protein

Mitochondrial enolase superfamily member 1

Gene

ENOSF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion (PubMed:24697329).1 Publication

Caution

Was originally (PubMed:8493092) identified as a gene coding for an antisense RNA to thymidylate synthase, and was proposed to down-regulate TYMS activity (PubMed:8869746), possibly by promoting the degradation of TYMS mRNA via an antisense RNA-based mechanism (PubMed:12084460).3 Publications

Catalytic activityi

L-fuconate = 2-dehydro-3-deoxy-L-fuconate + H2O.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

kcat is 0.5 sec(-1) for L-fuconate. kcat is 0.3 sec(-1) for L-galactonate. kcat is 0.3 sec(-1) for L-arabinonate. kcat is 0.04 sec(-1) for D-arabinonate. kcat is 0.002 sec(-1) for D-ribonate.
  1. KM=0.2 mM for L-fuconate1 Publication
  2. KM=3.0 mM for L-galactonate1 Publication
  3. KM=2.0 mM for D-arabinonate1 Publication
  4. KM=4.0 mM for L-arabinonate1 Publication
  5. KM=0.4 mM for D-ribonate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei34SubstrateBy similarity1
    Binding sitei220SubstrateBy similarity1
    Active sitei222Proton donor/acceptorBy similarity1
    Metal bindingi250Magnesium1 Publication1
    Binding sitei252SubstrateBy similarity1
    Metal bindingi276Magnesium1 Publication1
    Binding sitei276SubstrateBy similarity1
    Metal bindingi305Magnesium1 Publication1
    Binding sitei305SubstrateBy similarity1
    Active sitei355Sequence analysis1
    Binding sitei386SubstrateBy similarity1

    GO - Molecular functioni

    • isomerase activity Source: UniProtKB-KW
    • L-fuconate dehydratase activity Source: UniProtKB
    • magnesium ion binding Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase, Lyase
    LigandMagnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial enolase superfamily member 1 (EC:4.2.1.681 Publication)
    Alternative name(s):
    Antisense RNA to thymidylate synthase
    Short name:
    rTS
    L-fuconate dehydratase
    Gene namesi
    Name:ENOSF1
    Synonyms:RTS, TYMSAS
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 18

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000132199.18
    HGNCiHGNC:30365 ENOSF1
    MIMi607427 gene
    neXtProtiNX_Q7L5Y1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi1 – 27Missing : Impairs protein solubility. Abolishes catalytic activity. 1 PublicationAdd BLAST27

    Organism-specific databases

    DisGeNETi55556
    OpenTargetsiENSG00000132199
    PharmGKBiPA134897613

    Polymorphism and mutation databases

    BioMutaiENOSF1
    DMDMi74739173

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003316521 – 443Mitochondrial enolase superfamily member 1Add BLAST443

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei148Phosphoserine1 Publication1

    Post-translational modificationi

    Could be sumoylated.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    EPDiQ7L5Y1
    MaxQBiQ7L5Y1
    PaxDbiQ7L5Y1
    PeptideAtlasiQ7L5Y1
    PRIDEiQ7L5Y1

    PTM databases

    iPTMnetiQ7L5Y1
    PhosphoSitePlusiQ7L5Y1

    Expressioni

    Gene expression databases

    BgeeiENSG00000132199
    CleanExiHS_ENOSF1
    ExpressionAtlasiQ7L5Y1 baseline and differential
    GenevisibleiQ7L5Y1 HS

    Organism-specific databases

    HPAiHPA047829

    Interactioni

    Protein-protein interaction databases

    BioGridi120716, 7 interactors
    IntActiQ7L5Y1, 1 interactor
    STRINGi9606.ENSP00000345974

    Structurei

    Secondary structure

    1443
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 15Combined sources11
    Helixi18 – 20Combined sources3
    Beta strandi35 – 45Combined sources11
    Beta strandi51 – 57Combined sources7
    Helixi62 – 72Combined sources11
    Helixi73 – 75Combined sources3
    Turni76 – 78Combined sources3
    Helixi81 – 85Combined sources5
    Helixi88 – 96Combined sources9
    Helixi101 – 104Combined sources4
    Beta strandi106 – 108Combined sources3
    Helixi109 – 130Combined sources22
    Helixi134 – 140Combined sources7
    Helixi143 – 147Combined sources5
    Turni153 – 158Combined sources6
    Helixi161 – 170Combined sources10
    Turni171 – 174Combined sources4
    Helixi175 – 185Combined sources11
    Beta strandi187 – 191Combined sources5
    Helixi201 – 213Combined sources13
    Beta strandi218 – 222Combined sources5
    Helixi227 – 241Combined sources15
    Beta strandi245 – 250Combined sources6
    Helixi257 – 267Combined sources11
    Helixi268 – 270Combined sources3
    Beta strandi273 – 276Combined sources4
    Helixi284 – 294Combined sources11
    Helixi295 – 297Combined sources3
    Beta strandi300 – 303Combined sources4
    Helixi310 – 318Combined sources9
    Beta strandi323 – 325Combined sources3
    Turni329 – 331Combined sources3
    Helixi334 – 347Combined sources14
    Turni358 – 360Combined sources3
    Helixi361 – 375Combined sources15
    Beta strandi385 – 387Combined sources3
    Helixi392 – 394Combined sources3
    Beta strandi395 – 397Combined sources3
    Beta strandi400 – 407Combined sources8
    Beta strandi411 – 413Combined sources3
    Helixi420 – 426Combined sources7
    Turni428 – 430Combined sources3
    Helixi432 – 437Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4A35X-ray1.74A1-440[»]
    ProteinModelPortaliQ7L5Y1
    SMRiQ7L5Y1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni24 – 26Substrate bindingBy similarity3
    Regioni355 – 357Substrate bindingBy similarity3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410IET8 Eukaryota
    COG4948 LUCA
    GeneTreeiENSGT00390000014290
    HOGENOMiHOG000113757
    HOVERGENiHBG053904
    InParanoidiQ7L5Y1
    KOiK18334
    OMAiLVSCIDF
    OrthoDBiEOG091G027L
    PhylomeDBiQ7L5Y1
    TreeFamiTF300529

    Family and domain databases

    CDDicd03324 rTSbeta_L-fuconate_dehydratase, 1 hit
    Gene3Di3.20.20.120, 1 hit
    3.30.390.10, 1 hit
    InterProiView protein in InterPro
    IPR036849 Enolase-like_C_sf
    IPR029017 Enolase-like_N
    IPR029065 Enolase_C-like
    IPR034610 L-fuconate_dehydratase
    IPR018110 Mandel_Rmase/mucon_lact_enz_CS
    IPR034593 Mandelate_racemase-like
    IPR013342 Mandelate_racemase_C
    IPR013341 Mandelate_racemase_N_dom
    PANTHERiPTHR13794:SF58 PTHR13794:SF58, 1 hit
    PfamiView protein in Pfam
    PF13378 MR_MLE_C, 1 hit
    PF02746 MR_MLE_N, 1 hit
    SFLDiSFLDF00111 L-fuconate_dehydratase, 1 hit
    SFLDG00179 mandelate_racemase, 1 hit
    SMARTiView protein in SMART
    SM00922 MR_MLE, 1 hit
    SUPFAMiSSF51604 SSF51604, 1 hit
    PROSITEiView protein in PROSITE
    PS00909 MR_MLE_2, 1 hit

    Sequences (7)i

    Sequence statusi: Complete.

    This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q7L5Y1-1) [UniParc]FASTAAdd to basket
    Also known as: rTSgamma

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MVRGRISRLS VRDVRFPTSL GGHGADAMHT DPDYSAAYVV IETDAEDGIK
    60 70 80 90 100
    GCGITFTLGK GTEVVVCAVN ALAHHVLNKD LKDIVGDFRG FYRQLTSDGQ
    110 120 130 140 150
    LRWIGPEKGV VHLATAAVLN AVWDLWAKQE GKPVWKLLVD MDPRMLVSCI
    160 170 180 190 200
    DFRYITDVLT EEDALEILQK GQIGKKEREK QMLAQGYPAY TTSCAWLGYS
    210 220 230 240 250
    DDTLKQLCAQ ALKDGWTRFK VKVGADLQDD MRRCQIIRDM IGPEKTLMMD
    260 270 280 290 300
    ANQRWDVPEA VEWMSKLAKF KPLWIEEPTS PDDILGHATI SKALVPLGIG
    310 320 330 340 350
    IATGEQCHNR VIFKQLLQAK ALQFLQIDSC RLGSVNENLS VLLMAKKFEI
    360 370 380 390 400
    PVCPHAGGVG LCELVQHLII FDYISVSASL ENRVCEYVDH LHEHFKYPVM
    410 420 430 440
    IQRASYMPPK DPGYSTEMKE ESVKKHQYPD GEVWKKLLPA QEN
    Length:443
    Mass (Da):49,786
    Last modified:May 24, 2005 - v1
    Checksum:i82BEDAC229D1A729
    GO
    Isoform 2 (identifier: Q7L5Y1-2) [UniParc]FASTAAdd to basket
    Also known as: rTSalpha

    The sequence of this isoform differs from the canonical sequence as follows:
         1-102: Missing.
         103-103: W → MQKMESRGVELPSLWEKALKL

    Show »
    Length:361
    Mass (Da):41,024
    Checksum:i73B469F1C9C7E144
    GO
    Isoform 3 (identifier: Q7L5Y1-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         65-117: VVCAVNALAH...KGVVHLATAA → DWSRKGRGAP...GILHRFQVHH
         118-443: Missing.

    Show »
    Length:117
    Mass (Da):12,286
    Checksum:iB277BFA534E7DAF2
    GO
    Isoform 4 (identifier: Q7L5Y1-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-24: MVRGRISRLSVRDVRFPTSLGGHG → MVSADAMVSADAMVSADAMVSADAMVSADAMVSADAMVSADAMVS
         293-306: Missing.

    Note: Gene prediction based on EST data.
    Show »
    Length:450
    Mass (Da):50,170
    Checksum:i7D6BD13DF0F12AD0
    GO
    Isoform 5 (identifier: Q7L5Y1-5) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-24: MVRGRISRLSVRDVRFPTSLGGHG → MVSADAMVSA...AMVSADAMVS
         293-306: Missing.

    Show »
    Length:474
    Mass (Da):52,468
    Checksum:i97FA6018CAADF8CB
    GO
    Isoform 6 (identifier: Q7L5Y1-6) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-181: Missing.

    Show »
    Length:262
    Mass (Da):29,710
    Checksum:i5296CB2DCA806273
    GO
    Isoform 7 (identifier: Q7L5Y1-7) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-230: Missing.

    Show »
    Length:213
    Mass (Da):24,284
    Checksum:iF2CF0580E05E10BA
    GO

    Sequence cautioni

    The sequence AAG29537 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence CAA47471 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_04293331D → E. Corresponds to variant dbSNP:rs34724061Ensembl.1
    Natural variantiVAR_042934145M → T4 PublicationsCorresponds to variant dbSNP:rs2612086Ensembl.1
    Natural variantiVAR_042935428Y → S. Corresponds to variant dbSNP:rs2847620Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0552411 – 230Missing in isoform 7. 1 PublicationAdd BLAST230
    Alternative sequenceiVSP_0552421 – 181Missing in isoform 6. 1 PublicationAdd BLAST181
    Alternative sequenceiVSP_0333111 – 102Missing in isoform 2. 1 PublicationAdd BLAST102
    Alternative sequenceiVSP_0471531 – 24MVRGR…LGGHG → MVSADAMVSADAMVSADAMV SADAMVSADAMVSADAMVSA DAMVS in isoform 4. CuratedAdd BLAST24
    Alternative sequenceiVSP_0552431 – 24MVRGR…LGGHG → MVSADAMVSADAMVSADAMV SADAMVSADAMVSADAMVSA DAMVSADAMVSADAMVSADA MVSADAMVS in isoform 5. 1 PublicationAdd BLAST24
    Alternative sequenceiVSP_03331265 – 117VVCAV…LATAA → DWSRKGRGAPGDSGRPKRGV GLVGQAGGKACLEVTCGHGS QDAGILHRFQVHH in isoform 3. 1 PublicationAdd BLAST53
    Alternative sequenceiVSP_033313103W → MQKMESRGVELPSLWEKALK L in isoform 2. 1 Publication1
    Alternative sequenceiVSP_033314118 – 443Missing in isoform 3. 1 PublicationAdd BLAST326
    Alternative sequenceiVSP_047154293 – 306Missing in isoform 4 and isoform 5. 1 PublicationAdd BLAST14

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF305057 Genomic DNA Translation: AAG29536.1
    X67098 mRNA Translation: CAA47472.1
    AF305057 Genomic DNA Translation: AAG29537.1 Different initiation.
    X67098 mRNA Translation: CAA47471.1 Different initiation.
    X89602 mRNA Translation: CAA61761.1
    AK127818 mRNA Translation: BAC87148.1
    AK093873 mRNA Translation: BAG52778.1
    AK127219 mRNA Translation: BAG54456.1
    AK292780 mRNA Translation: BAF85469.1
    AP001178 Genomic DNA No translation available.
    CH471113 Genomic DNA Translation: EAX01713.1
    CH471113 Genomic DNA Translation: EAX01714.1
    CH471113 Genomic DNA Translation: EAX01715.1
    BC001285 mRNA Translation: AAH01285.2
    CCDSiCCDS11822.1 [Q7L5Y1-1]
    CCDS45821.1 [Q7L5Y1-2]
    RefSeqiNP_001119595.1, NM_001126123.3
    NP_001305688.1, NM_001318759.1
    NP_001305689.1, NM_001318760.1 [Q7L5Y1-6]
    NP_059982.2, NM_017512.5 [Q7L5Y1-1]
    NP_974487.1, NM_202758.3
    XP_016881324.1, XM_017025835.1
    XP_016881325.1, XM_017025836.1 [Q7L5Y1-6]
    UniGeneiHs.658550
    Hs.731510
    Hs.732707

    Genome annotation databases

    EnsembliENST00000251101; ENSP00000251101; ENSG00000132199 [Q7L5Y1-1]
    ENST00000340116; ENSP00000345974; ENSG00000132199 [Q7L5Y1-4]
    ENST00000383578; ENSP00000373072; ENSG00000132199 [Q7L5Y1-2]
    GeneIDi55556
    KEGGihsa:55556
    UCSCiuc002kkt.4 human [Q7L5Y1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiENOF1_HUMAN
    AccessioniPrimary (citable) accession number: Q7L5Y1
    Secondary accession number(s): A6NMP3
    , A8K9R5, B3KSL6, B3KXE4, D3DUH0, Q15407, Q15594, Q15595, Q6ZS08, Q9HAS5, Q9HAS6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 24, 2005
    Last modified: March 28, 2018
    This is version 112 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health