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Q7L5D6

- GET4_HUMAN

UniProt

Q7L5D6 - GET4_HUMAN

Protein

Golgi to ER traffic protein 4 homolog

Gene

GET4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.1 Publication

    GO - Biological processi

    1. tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
    2. transport Source: UniProtKB-KW

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgi to ER traffic protein 4 homolog
    Alternative name(s):
    Conserved edge-expressed protein
    Transmembrane domain recognition complex 35 kDa subunit
    Short name:
    TRC35
    Gene namesi
    Name:GET4
    Synonyms:C7orf20, CEE, TRC35
    ORF Names:CGI-20
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:21690. GET4.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. BAT3 complex Source: UniProtKB
    2. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA165618100.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed4 Publications
    Chaini2 – 327326Golgi to ER traffic protein 4 homologPRO_0000228104Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine4 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ7L5D6.
    PaxDbiQ7L5D6.
    PRIDEiQ7L5D6.

    PTM databases

    PhosphoSiteiQ7L5D6.

    Expressioni

    Gene expression databases

    BgeeiQ7L5D6.
    CleanExiHS_C7orf20.
    GenevestigatoriQ7L5D6.

    Organism-specific databases

    HPAiHPA019765.

    Interactioni

    Subunit structurei

    Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35.1 Publication

    Protein-protein interaction databases

    BioGridi119636. 25 interactions.
    IntActiQ7L5D6. 28 interactions.
    MINTiMINT-1852978.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7L5D6.
    SMRiQ7L5D6. Positions 23-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GET4 family.Curated

    Phylogenomic databases

    eggNOGiNOG252493.
    HOGENOMiHOG000264232.
    HOVERGENiHBG059877.
    InParanoidiQ7L5D6.
    OMAiLLNFISM.
    OrthoDBiEOG7PZRZ1.
    PhylomeDBiQ7L5D6.
    TreeFamiTF315163.

    Family and domain databases

    InterProiIPR007317. UPF0363.
    [Graphical view]
    PANTHERiPTHR12875. PTHR12875. 1 hit.
    PfamiPF04190. DUF410. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q7L5D6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAAAAMAEQ ESARNGGRNR GGVQRVEGKL RASVEKGDYY EAHQMYRTLF    50
    FRYMSQSKHT EARELMYSGA LLFFSHGQQN SAADLSMLVL ESLEKAEVEV 100
    ADELLENLAK VFSLMDPNSP ERVTFVSRAL KWSSGGSGKL GHPRLHQLLA 150
    LTLWKEQNYC ESRYHFLHSA DGEGCANMLV EYSTSRGFRS EVDMFVAQAV 200
    LQFLCLKNKS SASVVFTTYT QKHPSIEDGP PFVEPLLNFI WFLLLAVDGG 250
    KLTVFTVLCE QYQPSLRRDP MYNEYLDRIG QLFFGVPPKQ TSSYGGLLGN 300
    LLTSLMGSSE QEDGEESPSD GSPIELD 327

    Note: No experimental confirmation available.

    Length:327
    Mass (Da):36,504
    Last modified:July 5, 2004 - v1
    Checksum:i4D6C4233181B0512
    GO
    Isoform 2 (identifier: Q7L5D6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-53: Missing.

    Show »
    Length:274
    Mass (Da):30,523
    Checksum:iCDCA760B2B2E1473
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 2214EQESA…RNRGG → DRRAPATALQPRA in AAD27729. (PubMed:10810093)CuratedAdd
    BLAST
    Sequence conflicti177 – 1771N → D in AAD27729. (PubMed:10810093)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5353Missing in isoform 2. 2 PublicationsVSP_017652Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK024305 mRNA. Translation: BAG51289.1.
    AK097899 mRNA. Translation: BAG53546.1.
    AK125863 mRNA. Translation: BAG54258.1.
    AL133014 mRNA. Translation: CAB61355.1.
    CH236965 Genomic DNA. Translation: EAL23708.1.
    BC003550 mRNA. Translation: AAH03550.2.
    AF132954 mRNA. Translation: AAD27729.1.
    CCDSiCCDS5317.1. [Q7L5D6-1]
    PIRiT42648.
    RefSeqiNP_057033.2. NM_015949.2. [Q7L5D6-1]
    UniGeneiHs.107387.

    Genome annotation databases

    EnsembliENST00000265857; ENSP00000265857; ENSG00000239857. [Q7L5D6-1]
    ENST00000407192; ENSP00000385646; ENSG00000239857. [Q7L5D6-2]
    GeneIDi51608.
    KEGGihsa:51608.
    UCSCiuc003sjl.1. human. [Q7L5D6-1]

    Polymorphism databases

    DMDMi74738593.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK024305 mRNA. Translation: BAG51289.1 .
    AK097899 mRNA. Translation: BAG53546.1 .
    AK125863 mRNA. Translation: BAG54258.1 .
    AL133014 mRNA. Translation: CAB61355.1 .
    CH236965 Genomic DNA. Translation: EAL23708.1 .
    BC003550 mRNA. Translation: AAH03550.2 .
    AF132954 mRNA. Translation: AAD27729.1 .
    CCDSi CCDS5317.1. [Q7L5D6-1 ]
    PIRi T42648.
    RefSeqi NP_057033.2. NM_015949.2. [Q7L5D6-1 ]
    UniGenei Hs.107387.

    3D structure databases

    ProteinModelPortali Q7L5D6.
    SMRi Q7L5D6. Positions 23-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119636. 25 interactions.
    IntActi Q7L5D6. 28 interactions.
    MINTi MINT-1852978.

    PTM databases

    PhosphoSitei Q7L5D6.

    Polymorphism databases

    DMDMi 74738593.

    Proteomic databases

    MaxQBi Q7L5D6.
    PaxDbi Q7L5D6.
    PRIDEi Q7L5D6.

    Protocols and materials databases

    DNASUi 51608.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000265857 ; ENSP00000265857 ; ENSG00000239857 . [Q7L5D6-1 ]
    ENST00000407192 ; ENSP00000385646 ; ENSG00000239857 . [Q7L5D6-2 ]
    GeneIDi 51608.
    KEGGi hsa:51608.
    UCSCi uc003sjl.1. human. [Q7L5D6-1 ]

    Organism-specific databases

    CTDi 51608.
    GeneCardsi GC07P000916.
    H-InvDB HIX0033841.
    HIX0201122.
    HGNCi HGNC:21690. GET4.
    HPAi HPA019765.
    MIMi 612056. gene.
    neXtProti NX_Q7L5D6.
    PharmGKBi PA165618100.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG252493.
    HOGENOMi HOG000264232.
    HOVERGENi HBG059877.
    InParanoidi Q7L5D6.
    OMAi LLNFISM.
    OrthoDBi EOG7PZRZ1.
    PhylomeDBi Q7L5D6.
    TreeFami TF315163.

    Miscellaneous databases

    GeneWikii C7orf20.
    GenomeRNAii 51608.
    NextBioi 55506.
    PROi Q7L5D6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q7L5D6.
    CleanExi HS_C7orf20.
    Genevestigatori Q7L5D6.

    Family and domain databases

    InterProi IPR007317. UPF0363.
    [Graphical view ]
    PANTHERi PTHR12875. PTHR12875. 1 hit.
    Pfami PF04190. DUF410. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Ovary, Testis and Thymus.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    3. "Human chromosome 7: DNA sequence and biology."
      Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
      , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
      Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    5. Bienvenut W.V., Vousden K.H., Lukashchuk N.
      Submitted (MAR-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-14 AND 111-122, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Lung carcinoma.
    6. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
      Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
      Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-327 (ISOFORM 1).
    7. "Genomic, evolutionary, and expression analyses of cee, an ancient gene involved in normal growth and development."
      Fernandes J.M.O., Macqueen D.J., Lee H.-T., Johnston I.A.
      Genomics 91:315-325(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A ribosome-associating factor chaperones tail-anchored membrane proteins."
      Mariappan M., Li X., Stefanovic S., Sharma A., Mateja A., Keenan R.J., Hegde R.S.
      Nature 466:1120-1124(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE BAG6/BAT3 COMPLEX.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGET4_HUMAN
    AccessioniPrimary (citable) accession number: Q7L5D6
    Secondary accession number(s): A4D2Q1
    , B3KNC7, Q9UFC9, Q9Y309
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3