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Protein

Golgi to ER traffic protein 4 homolog

Gene

GET4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.1 Publication

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL

GO - Biological processi

  • cytoplasmic sequestering of protein Source: ParkinsonsUK-UCL
  • maintenance of unfolded protein involved in ERAD pathway Source: ParkinsonsUK-UCL
  • tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciZFISH:G66-31522-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 4 homolog
Alternative name(s):
Conserved edge-expressed protein
Transmembrane domain recognition complex 35 kDa subunit
Short name:
TRC35
Gene namesi
Name:GET4
Synonyms:C7orf20, CEE, TRC35
ORF Names:CGI-20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:21690. GET4.

Subcellular locationi

GO - Cellular componenti

  • BAT3 complex Source: UniProtKB
  • cytoplasm Source: ParkinsonsUK-UCL
  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi51608.
OpenTargetsiENSG00000239857.
PharmGKBiPA165618100.

Polymorphism and mutation databases

BioMutaiGET4.
DMDMi74738593.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002281042 – 327Golgi to ER traffic protein 4 homologAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ7L5D6.
MaxQBiQ7L5D6.
PaxDbiQ7L5D6.
PeptideAtlasiQ7L5D6.
PRIDEiQ7L5D6.

PTM databases

iPTMnetiQ7L5D6.
PhosphoSitePlusiQ7L5D6.

Expressioni

Gene expression databases

BgeeiENSG00000239857.
CleanExiHS_C7orf20.
ExpressionAtlasiQ7L5D6. baseline and differential.
GenevisibleiQ7L5D6. HS.

Organism-specific databases

HPAiHPA019765.

Interactioni

Subunit structurei

Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET4/TRC35.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ASNA1O436813EBI-711823,EBI-2515857
PRKAB2O437416EBI-711823,EBI-1053424

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi119636. 48 interactors.
IntActiQ7L5D6. 39 interactors.
MINTiMINT-1852978.
STRINGi9606.ENSP00000265857.

Structurei

3D structure databases

ProteinModelPortaliQ7L5D6.
SMRiQ7L5D6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GET4 family.Curated

Phylogenomic databases

eggNOGiKOG3024. Eukaryota.
ENOG410XRWE. LUCA.
GeneTreeiENSGT00390000015750.
HOGENOMiHOG000264232.
HOVERGENiHBG059877.
InParanoidiQ7L5D6.
OMAiEGQFYEA.
OrthoDBiEOG091G0DTD.
PhylomeDBiQ7L5D6.
TreeFamiTF315163.

Family and domain databases

InterProiIPR007317. UPF0363.
[Graphical view]
PANTHERiPTHR12875. PTHR12875. 1 hit.
PfamiPF04190. DUF410. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7L5D6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAMAEQ ESARNGGRNR GGVQRVEGKL RASVEKGDYY EAHQMYRTLF
60 70 80 90 100
FRYMSQSKHT EARELMYSGA LLFFSHGQQN SAADLSMLVL ESLEKAEVEV
110 120 130 140 150
ADELLENLAK VFSLMDPNSP ERVTFVSRAL KWSSGGSGKL GHPRLHQLLA
160 170 180 190 200
LTLWKEQNYC ESRYHFLHSA DGEGCANMLV EYSTSRGFRS EVDMFVAQAV
210 220 230 240 250
LQFLCLKNKS SASVVFTTYT QKHPSIEDGP PFVEPLLNFI WFLLLAVDGG
260 270 280 290 300
KLTVFTVLCE QYQPSLRRDP MYNEYLDRIG QLFFGVPPKQ TSSYGGLLGN
310 320
LLTSLMGSSE QEDGEESPSD GSPIELD
Note: No experimental confirmation available.
Length:327
Mass (Da):36,504
Last modified:July 5, 2004 - v1
Checksum:i4D6C4233181B0512
GO
Isoform 2 (identifier: Q7L5D6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:274
Mass (Da):30,523
Checksum:iCDCA760B2B2E1473
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 22EQESA…RNRGG → DRRAPATALQPRA in AAD27729 (PubMed:10810093).CuratedAdd BLAST14
Sequence conflicti177N → D in AAD27729 (PubMed:10810093).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0176521 – 53Missing in isoform 2. 2 PublicationsAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024305 mRNA. Translation: BAG51289.1.
AK097899 mRNA. Translation: BAG53546.1.
AK125863 mRNA. Translation: BAG54258.1.
AL133014 mRNA. Translation: CAB61355.1.
CH236965 Genomic DNA. Translation: EAL23708.1.
BC003550 mRNA. Translation: AAH03550.2.
AF132954 mRNA. Translation: AAD27729.1.
CCDSiCCDS5317.1. [Q7L5D6-1]
PIRiT42648.
RefSeqiNP_057033.2. NM_015949.2. [Q7L5D6-1]
UniGeneiHs.107387.
Hs.743756.

Genome annotation databases

EnsembliENST00000265857; ENSP00000265857; ENSG00000239857. [Q7L5D6-1]
ENST00000407192; ENSP00000385646; ENSG00000239857. [Q7L5D6-2]
GeneIDi51608.
KEGGihsa:51608.
UCSCiuc003sjl.2. human. [Q7L5D6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024305 mRNA. Translation: BAG51289.1.
AK097899 mRNA. Translation: BAG53546.1.
AK125863 mRNA. Translation: BAG54258.1.
AL133014 mRNA. Translation: CAB61355.1.
CH236965 Genomic DNA. Translation: EAL23708.1.
BC003550 mRNA. Translation: AAH03550.2.
AF132954 mRNA. Translation: AAD27729.1.
CCDSiCCDS5317.1. [Q7L5D6-1]
PIRiT42648.
RefSeqiNP_057033.2. NM_015949.2. [Q7L5D6-1]
UniGeneiHs.107387.
Hs.743756.

3D structure databases

ProteinModelPortaliQ7L5D6.
SMRiQ7L5D6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119636. 48 interactors.
IntActiQ7L5D6. 39 interactors.
MINTiMINT-1852978.
STRINGi9606.ENSP00000265857.

PTM databases

iPTMnetiQ7L5D6.
PhosphoSitePlusiQ7L5D6.

Polymorphism and mutation databases

BioMutaiGET4.
DMDMi74738593.

Proteomic databases

EPDiQ7L5D6.
MaxQBiQ7L5D6.
PaxDbiQ7L5D6.
PeptideAtlasiQ7L5D6.
PRIDEiQ7L5D6.

Protocols and materials databases

DNASUi51608.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265857; ENSP00000265857; ENSG00000239857. [Q7L5D6-1]
ENST00000407192; ENSP00000385646; ENSG00000239857. [Q7L5D6-2]
GeneIDi51608.
KEGGihsa:51608.
UCSCiuc003sjl.2. human. [Q7L5D6-1]

Organism-specific databases

CTDi51608.
DisGeNETi51608.
GeneCardsiGET4.
H-InvDBHIX0033841.
HIX0201122.
HGNCiHGNC:21690. GET4.
HPAiHPA019765.
MIMi612056. gene.
neXtProtiNX_Q7L5D6.
OpenTargetsiENSG00000239857.
PharmGKBiPA165618100.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3024. Eukaryota.
ENOG410XRWE. LUCA.
GeneTreeiENSGT00390000015750.
HOGENOMiHOG000264232.
HOVERGENiHBG059877.
InParanoidiQ7L5D6.
OMAiEGQFYEA.
OrthoDBiEOG091G0DTD.
PhylomeDBiQ7L5D6.
TreeFamiTF315163.

Enzyme and pathway databases

BioCyciZFISH:G66-31522-MONOMER.

Miscellaneous databases

GeneWikiiC7orf20.
GenomeRNAii51608.
PROiQ7L5D6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000239857.
CleanExiHS_C7orf20.
ExpressionAtlasiQ7L5D6. baseline and differential.
GenevisibleiQ7L5D6. HS.

Family and domain databases

InterProiIPR007317. UPF0363.
[Graphical view]
PANTHERiPTHR12875. PTHR12875. 1 hit.
PfamiPF04190. DUF410. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGET4_HUMAN
AccessioniPrimary (citable) accession number: Q7L5D6
Secondary accession number(s): A4D2Q1
, B3KNC7, Q9UFC9, Q9Y309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.