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Protein

Golgi to ER traffic protein 4 homolog

Gene

GET4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.1 Publication

GO - Biological processi

  • tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 4 homolog
Alternative name(s):
Conserved edge-expressed protein
Transmembrane domain recognition complex 35 kDa subunit
Short name:
TRC35
Gene namesi
Name:GET4
Synonyms:C7orf20, CEE, TRC35
ORF Names:CGI-20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:21690. GET4.

Subcellular locationi

GO - Cellular componenti

  • BAT3 complex Source: UniProtKB
  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165618100.

Polymorphism and mutation databases

BioMutaiGET4.
DMDMi74738593.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed4 Publications
Chaini2 – 327326Golgi to ER traffic protein 4 homologPRO_0000228104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine4 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ7L5D6.
PaxDbiQ7L5D6.
PRIDEiQ7L5D6.

PTM databases

PhosphoSiteiQ7L5D6.

Expressioni

Gene expression databases

BgeeiQ7L5D6.
CleanExiHS_C7orf20.
ExpressionAtlasiQ7L5D6. baseline.
GenevestigatoriQ7L5D6.

Organism-specific databases

HPAiHPA019765.

Interactioni

Subunit structurei

Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKAB2O437414EBI-711823,EBI-1053424

Protein-protein interaction databases

BioGridi119636. 34 interactions.
IntActiQ7L5D6. 29 interactions.
MINTiMINT-1852978.

Structurei

3D structure databases

ProteinModelPortaliQ7L5D6.
SMRiQ7L5D6. Positions 23-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GET4 family.Curated

Phylogenomic databases

eggNOGiNOG252493.
GeneTreeiENSGT00390000015750.
HOGENOMiHOG000264232.
HOVERGENiHBG059877.
InParanoidiQ7L5D6.
OMAiIQEMIGW.
OrthoDBiEOG7PZRZ1.
PhylomeDBiQ7L5D6.
TreeFamiTF315163.

Family and domain databases

InterProiIPR007317. UPF0363.
[Graphical view]
PANTHERiPTHR12875. PTHR12875. 1 hit.
PfamiPF04190. DUF410. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7L5D6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAMAEQ ESARNGGRNR GGVQRVEGKL RASVEKGDYY EAHQMYRTLF
60 70 80 90 100
FRYMSQSKHT EARELMYSGA LLFFSHGQQN SAADLSMLVL ESLEKAEVEV
110 120 130 140 150
ADELLENLAK VFSLMDPNSP ERVTFVSRAL KWSSGGSGKL GHPRLHQLLA
160 170 180 190 200
LTLWKEQNYC ESRYHFLHSA DGEGCANMLV EYSTSRGFRS EVDMFVAQAV
210 220 230 240 250
LQFLCLKNKS SASVVFTTYT QKHPSIEDGP PFVEPLLNFI WFLLLAVDGG
260 270 280 290 300
KLTVFTVLCE QYQPSLRRDP MYNEYLDRIG QLFFGVPPKQ TSSYGGLLGN
310 320
LLTSLMGSSE QEDGEESPSD GSPIELD

Note: No experimental confirmation available.

Length:327
Mass (Da):36,504
Last modified:July 5, 2004 - v1
Checksum:i4D6C4233181B0512
GO
Isoform 2 (identifier: Q7L5D6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:274
Mass (Da):30,523
Checksum:iCDCA760B2B2E1473
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 2214EQESA…RNRGG → DRRAPATALQPRA in AAD27729 (PubMed:10810093).CuratedAdd
BLAST
Sequence conflicti177 – 1771N → D in AAD27729 (PubMed:10810093).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5353Missing in isoform 2. 2 PublicationsVSP_017652Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024305 mRNA. Translation: BAG51289.1.
AK097899 mRNA. Translation: BAG53546.1.
AK125863 mRNA. Translation: BAG54258.1.
AL133014 mRNA. Translation: CAB61355.1.
CH236965 Genomic DNA. Translation: EAL23708.1.
BC003550 mRNA. Translation: AAH03550.2.
AF132954 mRNA. Translation: AAD27729.1.
CCDSiCCDS5317.1. [Q7L5D6-1]
PIRiT42648.
RefSeqiNP_057033.2. NM_015949.2. [Q7L5D6-1]
UniGeneiHs.107387.

Genome annotation databases

EnsembliENST00000265857; ENSP00000265857; ENSG00000239857. [Q7L5D6-1]
ENST00000407192; ENSP00000385646; ENSG00000239857. [Q7L5D6-2]
GeneIDi51608.
KEGGihsa:51608.
UCSCiuc003sjl.1. human. [Q7L5D6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024305 mRNA. Translation: BAG51289.1.
AK097899 mRNA. Translation: BAG53546.1.
AK125863 mRNA. Translation: BAG54258.1.
AL133014 mRNA. Translation: CAB61355.1.
CH236965 Genomic DNA. Translation: EAL23708.1.
BC003550 mRNA. Translation: AAH03550.2.
AF132954 mRNA. Translation: AAD27729.1.
CCDSiCCDS5317.1. [Q7L5D6-1]
PIRiT42648.
RefSeqiNP_057033.2. NM_015949.2. [Q7L5D6-1]
UniGeneiHs.107387.

3D structure databases

ProteinModelPortaliQ7L5D6.
SMRiQ7L5D6. Positions 23-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119636. 34 interactions.
IntActiQ7L5D6. 29 interactions.
MINTiMINT-1852978.

PTM databases

PhosphoSiteiQ7L5D6.

Polymorphism and mutation databases

BioMutaiGET4.
DMDMi74738593.

Proteomic databases

MaxQBiQ7L5D6.
PaxDbiQ7L5D6.
PRIDEiQ7L5D6.

Protocols and materials databases

DNASUi51608.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265857; ENSP00000265857; ENSG00000239857. [Q7L5D6-1]
ENST00000407192; ENSP00000385646; ENSG00000239857. [Q7L5D6-2]
GeneIDi51608.
KEGGihsa:51608.
UCSCiuc003sjl.1. human. [Q7L5D6-1]

Organism-specific databases

CTDi51608.
GeneCardsiGC07P000916.
H-InvDBHIX0033841.
HIX0201122.
HGNCiHGNC:21690. GET4.
HPAiHPA019765.
MIMi612056. gene.
neXtProtiNX_Q7L5D6.
PharmGKBiPA165618100.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG252493.
GeneTreeiENSGT00390000015750.
HOGENOMiHOG000264232.
HOVERGENiHBG059877.
InParanoidiQ7L5D6.
OMAiIQEMIGW.
OrthoDBiEOG7PZRZ1.
PhylomeDBiQ7L5D6.
TreeFamiTF315163.

Miscellaneous databases

GeneWikiiC7orf20.
GenomeRNAii51608.
NextBioi55506.
PROiQ7L5D6.
SOURCEiSearch...

Gene expression databases

BgeeiQ7L5D6.
CleanExiHS_C7orf20.
ExpressionAtlasiQ7L5D6. baseline.
GenevestigatoriQ7L5D6.

Family and domain databases

InterProiIPR007317. UPF0363.
[Graphical view]
PANTHERiPTHR12875. PTHR12875. 1 hit.
PfamiPF04190. DUF410. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Ovary, Testis and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  5. Bienvenut W.V., Vousden K.H., Lukashchuk N.
    Submitted (MAR-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-14 AND 111-122, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Lung carcinoma.
  6. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-327 (ISOFORM 1).
  7. "Genomic, evolutionary, and expression analyses of cee, an ancient gene involved in normal growth and development."
    Fernandes J.M.O., Macqueen D.J., Lee H.-T., Johnston I.A.
    Genomics 91:315-325(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  9. "A ribosome-associating factor chaperones tail-anchored membrane proteins."
    Mariappan M., Li X., Stefanovic S., Sharma A., Mateja A., Keenan R.J., Hegde R.S.
    Nature 466:1120-1124(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE BAG6/BAT3 COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGET4_HUMAN
AccessioniPrimary (citable) accession number: Q7L5D6
Secondary accession number(s): A4D2Q1
, B3KNC7, Q9UFC9, Q9Y309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: May 27, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.